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Provedor de dados:  Planta Daninha
País:  Brazil
Título:  Resistance detection of blackjack to ALS inhibitors by in vitro plant growth method
Autores:  Tessaro,Daniela
T. Perboni,Lais
R. Garcia,Jéssica
M. Teixeira,Marlon
Data:  2020-01-01
Ano:  2020
Palavras-chave:  Bidens Pilosa
Resistance test
Acetolactate synthase
Resumo:  Abstract Background: The resistance of weeds to herbicides is a problem that has been increasingly studied because of its great importance in agriculture and rapid growth of this phenomenon worldwide. The quick detection of resistant plants is extremely important for resistance management. Weed resistance to herbicides can be detected through field, greenhouse and laboratory tests. A fast way of detecting resistance is using tissue culture method, where is possible to use resistant plant clones and obtain results faster than whole-plant method in greenhouse. Objective: The aims of the research were to detect the resistance of blackjack to herbicides inhibitors of the acetolactate synthase (ALS), addition in vitro cultivation; determine the herbicide concentration of imazethapyr to 95% control of the susceptible plant population; evaluate explants growth; and, determine the herbicide concentration added to the culture medium where is possible to distinguish a resistant from a susceptible plant. Methods: Experiments were carried out in greenhouse and tissue culture laboratory. Screening was performed to select resistant and susceptible biotypes, tests for specie establishment and dose response curves in vitro were made. Results: The herbicide concentration added to the culture medium that provided 95% susceptible biotype control and efficiently differentiated susceptible biotype from resistant one was 0.6μM of imazethapyr. Conclusions: This method helps recommendations of weed management and provides a quick decision to alternative control of this specie, thus avoiding major damage to the crops.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Editor:  Sociedade Brasileira da Ciência das Plantas Daninhas
Relação:  10.1590/s0100-83582020380100065
Formato:  text/html
Fonte:  Planta Daninha v.38 2020
Direitos:  info:eu-repo/semantics/openAccess

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