| Registro completo |
| Provedor de dados: |
Rev. Bras. Bot.
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| País: |
Brazil
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| Título: |
Comparative uptake and metabolism of 2-[14C]-2,4-dichlorophenoxyacetic acid in callus cultures of monocot (Dioscorea spp.) and dicot (Nicotiana tabacum L.) plants
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| Autores: |
Viana,Ana Maria
Mantell,Sinclair Hugh
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| Data: |
1998-04-01
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| Ano: |
1998
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| Palavras-chave: |
Dioscorea spp. Nicotiana tabacum plant tissue culture 2
4-dichlorophenoxyacetic acid (2
4-D)-uptake auxin metabolism radioactively labelled 2
4-D
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| Resumo: |
(Comparative uptake and metabolism of 2-[14C]-2,4-dichlorophenoxyacetic acid in callus cultures of monocot (Dioscorea spp.) and dicot (Nicotiana tabacum L.) plants). The uptake and metabolism of 2-[14C]-2,4-dichlorophenoxyacetic acid (2,4-D) were investigated in leaf calluses of Nicotiana tabacum, tuber calluses of Dioscorea opposita and calluses derived from zygotic embryos, leaves and petioles of Dioscorea composita. Striking similarities were evident in the patterns of 2,4-D metabolites and their chemical characteristics in the three callus types of D. composita compared, but significant differences were detected among the patterns of rnetabolites in the three species studied. Preliminary investigations on the stability of various metabolites (separated using TLC) by hydrolysis showed that sugar esters appeared to be the major metabolites in tobacco whilst in yams (D. opposita) glycosides were shown to be the main ones, which indicated a similarity between plants of Gramineae and Dioscoreaceae in terms of 2,4-D metabolism. Release of 2,4-D from tobacco callus cells upon their transfer to 2,4-D-free medium was detected and the implications of this are discussed in relation to the cultural conditions necessary to induce morphogenesis in vitro.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041998000100009
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| Editor: |
Sociedade Botânica de São Paulo
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| Relação: |
10.1590/S0100-84041998000100009
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Botany v.21 n.1 1998
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| Direitos: |
info:eu-repo/semantics/openAccess
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