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Provedor de dados:  Plantas Medicinales
País:  Cuba
Título:  Rollinia mucosa (Jacq.) Baill.: establishment of callus culture and lignan production
Autores:  Lua Figueiredo,Solange Faria
Campos Viana,Vera Regina
Simões,Cláudia
Trugo,Luiz Carlos
Coelho Kaplan,Maria Auxiliadora
Data:  2003-12-01
Ano:  2003
Palavras-chave:  ROLLINIA
CULTURE MEDIA
Resumo:  Callus cultures were established from different organs of seedlings and in vitro propagated plants of Rollinia mucosa. The growth rate, type of callus and furofuranic lignan biosynthetic pattern were significantly influenced by the origin of the plant material, the explant type and the growth regulators used: 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, 6-benzyladenine, gibberellic acid, and picloram.The efficiency of callus production and lignan synthesis was significantly higher in foliar blade explants in the majority of media. The best biomass was obtained on media with picloram. In seedlings explants, naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid induced epiyangambin synthesis in calli from foliar blade and magnolin in calli from epicotyl and petiole. In in vitro propagated plants explants, the synthesis of epiyangambin was induced by picloram only in calli from stem. Calli from foliar blade cultured in 10,4, 20,8 and 31,2 µM of picloram presented a two-three fold increase in epiyangambin rates with regard to levels detected in the original plant.
Tipo:  Journal article
Idioma:  Espanhol
Identificador:  http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962003000300007
Editor:  ECIMED
Formato:  text/html
Fonte:  Revista Cubana de Plantas Medicinales v.8 n.3 2003
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