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| Registros recuperados: 38 | |
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| Derolez, Valerie; Soudant, Dominique; Malet, Nathalie; Chiantella, Claude; Richard, Marion; Abadie, Eric; Aliaume, Catherine; Bec, Beatrice. |
| Mediterranean costal lagoons have been exposed to anthropic eutrophication for decades. Thau lagoon is one of the largest among them and provides many ecosystem services including shellfish farming. Considerable efforts were made between the 1970s and the late 2000s to improve the wastewater treatment systems in the lagoon watershed. A decrease in nutrient inputs to Thau lagoon was subsequently observed, evidence for the start of a recovery process in Thau lagoon, following oligotrophication. This decrease has significant consequences for ecosystem communities, above all primary producers. In our study, we characterised and quantified long-term changes by analysing long monitoring time-series (1998-2016) of phytoplankton biomass, abundance and composition... |
| Tipo: Text |
Palavras-chave: Eutrophication; Oligotrophication; Nutrients; Recovery; Phytoplankton community shift; Mediterranean coastal lagoon. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74063/73514.pdf |
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| Hadjadji, Imen; Laabir, Mohamed; Frihi, Hocine; Collos, Yves; Shao, Zhao Jun; Berrebi, Patrick; Abadie, Eric; Amzil, Zouher; Chomérat, Nicolas; Rolland, Jean-luc; Rieuvilleneuve, Fabien; Masseret, Estelle. |
| Physiological plasticity gives HABs species the ability to respond to variations in the surrounding environment. The aim of this study was to examine morphological and physiological variability in Alexandrium pacificum R.W. Litaker (Group IV) (former Alexandrium catenella) blooming in Annaba bay, Algeria. Monoclonal cultures of up to 30 strains of this neurotoxic dinoflagellate were established by the germination of single resting cysts from the surface sediment of this southern Mediterranean marine ecosystem. Ribotyping confirmed formally for the first time that A. pacificum is developing in Eastern Algerian waters. Toxin analyses of A. pacificum strains revealed substantial intraspecific variability in both the profile and toxin amount. However, the... |
| Tipo: Text |
Palavras-chave: Alexandrium pacificum; Mediterranean; Toxin profile; Growth; Intraspecific variability. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00624/73620/73598.pdf |
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| Zendong, Zita; Herrenknecht, Christine; Abadie, Eric; Brissard, Charline; Tixier, Celine; Mondeguer, Florence; Sechet, Veronique; Amzil, Zouher; Hess, Philipp. |
| Marine biotoxins are algal metabolites that can accumulate in fish or shellfish and render these foodstuffs unfit for human consumption. These toxins, released into seawater during algal occurrences, can be monitored through passive sampling. Acetone, methanol and isopropanol were evaluated for their efficiency in extracting toxins from algal biomass. Isopropanol was chosen for further experiments thanks to a slightly higher recovery and no artifact formation. Comparison of Oasis HLB, Strata-X, BondElut C18 and HP-20 sorbent materials in SPE-mode led to the choice of Oasis HLB, HP-20 and Strata-X. These three sorbents were separately exposed as passive samplers for 24 h to seawater spiked with algal extracts containing known amounts of okadaic acid (OA),... |
| Tipo: Text |
Palavras-chave: Passive sampling; Oasis HLB; Strata-X; LOPE; PDMS; SPATT. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00186/29756/28187.pdf |
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| Delcourt, Nicolas; Lagrange, Emmeline; Abadie, Eric; Fessard, Valérie; Frémy, Jean-marc; Vernoux, Jean-paul; Peyrat, Marie-bénédicte; Maignien, Thomas; Arnich, Nathalie; Molgó, Jordi; Mattei, César. |
| Pinnatoxins (PnTXs) are emerging neurotoxins that were discovered about 30 years ago. They are solely produced by the marine dinoflagellate Vulcanodinium rugosum, and may be transferred into the food chain, as they have been found in various marine invertebrates, including bivalves. No human intoxication has been reported to date although acute toxicity was induced by PnTxs in rodents. LD50 values have been estimated for the different PnTXs through the oral route. At sublethal doses, all symptoms are reversible, and no neurological sequelae are visible. These symptoms are consistent with impairment of central and peripheral cholinergic network functions. In fact, PnTXs are high-affinity competitive antagonists of nicotinic acetylcholine receptors (nAChRs).... |
| Tipo: Text |
Palavras-chave: Pinnatoxins; Cyclic imines; Vulcanodinium rugosum; Nicotinic acetylcholine receptors; Acute neurotoxicity; Human intoxication; Myasthenia gravis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00507/61883/65958.pdf |
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| Rolland, Jean-luc; Medhioub, Walid; Vergnes, Agnes; Abi-khalil, Celina; Savar, Veronique; Abadie, Eric; Masseret, Estelle; Amzil, Zouher; Laabir, Mohamed. |
| To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Biomarker; Expression; Phytoplankton. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00225/33606/32003.pdf |
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| Hadjadji, Imene; Frehi, Hocine; Ayada, Lembarek; Abadie, Eric; Collos, Yves. |
| Environmental conditions ultimately leading to blooms of the toxic dinoflagellate Alexandrium catenella/tamarense were investigated at two Mediterranean sites (Annaba Bay, Algeria and Thau lagoon, France). Three years were examined in details: 1992 (a pre-Alexandrium period), 2002 (a year with the first bloom in Annaba) and 2010 (a year with a major bloom in Annaba). Most conditions were similar, but ammonium concentrations were much higher in Annaba (up to 100 μM) than in Thau (up to 10 μM). First records of A. catenella/tamarense were in 1995 for Thau and 2002 for Annaba, and coincided with soluble reactive phosphorus (SRP) decreasing below a concentration of about 1 μM. No other environmental variable could be related to those blooms. Thus, it is likely... |
| Tipo: Text |
Palavras-chave: Alexandrium catenella/tamarense; Blooms; Algeria; France; Mediterranean. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00174/28574/26995.pdf |
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| Serais, Ophelie; Abadie, Eric; Hubert, Clarisse; Bellamy, Elise; Fiandrino, Annie; Lemoine, Maud; Neaud-masson, Nadine. |
| Ce rapport a été réalisé à la demande de la Direction Départementale de la Protection des Populations de l’Hérault (DDPP34). Les résultats en toxines lipophiles réglementées (Acide Okadaïque, Dinophysis toxines et Pectenotoxines uniquement) dans les moules et les huîtres prélevées au point « Parc Leucate 2 » dans le cadre du REPHYTOX, ainsi que les dénombrements du phytoplancton toxique du genre Dinophysis réalisés dans le cadre du REPHY dans la lagune de Leucate, sont présentés sur des graphiques permettant une visualisation temporelle des épisodes de contamination entre janvier 2014 et décembre 2020. |
| Tipo: Text |
Palavras-chave: REPHYTOX; REPHY; Leucate; Point Parc Leucate 2; Moules; Huîtres; Toxins lipophiles; Dinophysis; Graphiques; Période 2014-2020. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77444/79099.pdf |
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| Amzil, Zouher; Sibat, Manoella; Royer, Florence; Masson, Nadine; Abadie, Eric. |
| In the context of the French Phytoplankton and Phycotoxins Monitoring Network (REPHY) programme, shellfish samples were harvested from different locations where harmful algae blooms were known to have occurred. For all shellfish samples found positive by the mouse bioassay for diarrhetic shellfish poisoning (DSP) toxins, liquid chromatography (LC) coupled with mass spectrometry (MS) was used to search for the following lipophilic toxins: okadaic acid (OA), dinophysistoxins (DTXs), pectenotoxins (PTXs), azaspiracids (AZAs), yessotoxins (YTXs), spirolides (SPXs) and gymnodimines (GYMs). In order to investigate the presence of acyl-OAs and/or acyl-DTX-1,-2 (DTX-3), alkaline hydrolysis was performed on all samples, and LC/ MS analyses were carried out on the... |
| Tipo: Text |
Palavras-chave: LC/MS analysis; Pectenotoxins; Spirolides; DSP toxins. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-6206.pdf |
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| Collos, Y; Vaquer, A; Laabir, M; Abadie, Eric; Laugier, Thierry; Pastoureaud, Annie; Souchu, Philippe. |
| A monitoring program with a weekly sampling frequency over a 15-month period indicates that urea concentrations above a certain threshold level may trigger the blooms of Alexandrium catenella in Thau lagoon. However, urea concentrations were also sometimes related to ammonium and dissolved organic nitrogen concentrations, indicating that the role of urea may not be a direct one. An original approach is used to assess the relative contribution of several nitrogen sources (nitrate, nitrite, ammonium, urea) to growth of A. catenella by comparing nitrogen uptake rates to nitrogen-based growth rates estimated from dilution experiments during four blooms over a 4-year period (2001-2004) in Thau lagoon. Nitrate and nitrite contributed 0.1-14% and 0.1-5%... |
| Tipo: Text |
Palavras-chave: Uptake kinetics; Ammonium; Urea; Growth; Nitrogen; HAB; Alexandrium catenella. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-6454.pdf |
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| Arnich, Nathalie; Abadie, Eric; Delcourt, Nicolas; Fessard, Valérie; Fremy, Jean-marc; Hort, Vincent; Lagrange, Emmeline; Maignien, Thomas; Molgó, Jordi; Peyrat, Marie-bénédicte; Vernoux, Jean-paul; Mattei, César. |
| Pinnatoxins (PnTXs) are a group of emerging marine biotoxins produced by the benthic dinoflagellate Vulcanodinium rugosum, currently not regulated in Europe or in any other country in the world. In France, PnTXs were detected for the first time in 2011, in mussels from the Ingril lagoon (South of France, Mediterranean coast). Since then, analyses carried out in mussels from this lagoon have shown high concentrations of PnTXs for several months each year. PnTXs have also been detected, to a lesser extent, in mussels from other Mediterranean lagoons and on the Atlantic and Corsican coasts. In the French data, the main analog is PnTX G (low levels of PnTX A are also present in some samples). No cases of PnTXs poisoning in humans have been reported so far in... |
| Tipo: Text |
Palavras-chave: Pinnatoxins; Shellfish; Emerging marine biotoxins; Risk assessment. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00619/73105/72236.pdf |
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| Abadie, Eric. |
| Harmful and / or toxic phytoplankton blooms impact for a long time marine ecosystems worldwide. These massive developments have an adverse effect on ecosystems and their exploitation. The lagoons of the region Languedoc-Roussillon are affected for decades. In addition to recurring health risks from Dinophysis (DSP toxins) and Alexandrium (PSP toxins), Vulcanodinium rugosum was identified in 2011 in the lagoon Ingril. This new species product pinnatoxins (neurotoxins). Through this thesis work, the biology of this new species (life cycle, growth condition, toxin production), its shellfish contamination capacity and geographic distribution in neighboring lagoons were studied on cultures in the laboratory and via environmental monitoring. The results of this... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00473/58425/61002.pdf |
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| Crottier, Anais; Abadie, Eric; Brun, Melanie; Piquet, Jean-come; Fiandrino, Annie. |
| Après un rappel des objectifs, du fonctionnement et de la méthode d’interprétation des résultats des réseaux de contrôle microbiologique (REMI) et de surveillance chimique (ROCCH), ce rapport inclut un bilan national et décrit le programme annuel des départements de l’Hérault, du Gard et de l’Aude. Il présente l’ensemble des résultats obtenus, en particulier l’estimation de la qualité microbiologique et chimique des zones de production de coquillages classées pour la période 2015-2017. Sur les 29 zones de production conchylicole classées et suivies en Occitanie, la qualité a pu être estimée pour 23 d’entre elles. Selon les critères réglementaires pris en compte (règlement CE n°854/2004, modifié par le règlement n°2285/2015), dans les départements de... |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Classement sanitaire des zones de production; Département de l’Hérault; Départemen tdu Gard; Département de l’Aude. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00445/55635/57280.pdf |
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| Marzin, Anahita; Crottier, Anais; Piquet, Jean-come; Abadie, Eric; Roque D'Orbcastel, Emmanuelle; Fiandrino, Annie. |
| L’étude de zone des lotissements conchylicoles de la lagune de Thau (34.39) a été menée, à la demande du Comité Régional de la Conchyliculture en Méditerranée et du préfet de l’Hérault, pour étudier la possibilité de sectoriser la zone de production en sous-zones, au regard du risque microbiologiques (REMI). Une étude de dossier a été réalisée afin de recenser les principales sources de contamination. Celles-ci sont essentiellement d’origine anthropique et impactent différemment les secteurs envisagés. Des prélèvements de coquillages du groupe 3 ont été réalisés de mai 2017 à mai 2018 avec une fréquence bimensuelle. Au regard des critères du règlement (CE) n° 854/2004, les résultats des analyses microbiologiques (indicateur Escherichia coli) ont permis... |
| Tipo: Text |
Palavras-chave: REMI; E. coli; Contamination bactériologique des coquillages; ROCCH; Contaminants chimiques; Milieu marin; Étude de zone; Lagune de Thau; Département de l’Hérault. |
| Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00459/57056/58965.pdf |
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| Abadie, Eric; Chiantella, Claude; Crottier, Anais; Rhodes, Lesley; Masseret, Estelle; Berteaux, Tom; Laabir, Mohamed. |
| Vulcanodinium rugosum, a dinoflagellate developing in Ingril Lagoon (Mediterranean, France) is responsible for shellfish intoxications due to the neurotoxin pinnatoxin G. A one year survey (March 2012–April 2013) was conducted in this oligotrophic shallow lagoon and key environmental parameters were recorded (temperature, salinity and nutrients). The spatio-temporal distribution of V. rugosum in water column and on macrophytes was also determined. Planktonic cells of V. rugosum were observed at all sampling stations, but in relatively low concentrations (maximum of 1000 cell/L). The highest abundances were observed from June to September 2012. There was a positive correlation between cell densities and both temperature and salinity. Non-motile cells were... |
| Tipo: Text |
Palavras-chave: Vulcanodinium rugosum; Neurotoxins; Ingril lagoon; Environmental factors; Development dynamic. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00437/54862/56478.pdf |
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| Laabir, Mohamed; Collos, Yves; Masseret, Estelle; Grzebyk, Daniel; Abadie, Eric; Savar, Veronique; Sibat, Manoella; Amzil, Zouher. |
| Laboratory experiments were designed to study the toxin content and profile of the Alexandrium catenella strain ACT03 (isolated from Thau Lagoon, French Mediterranean) in response to abiotic environmental factors under nutrient-replete conditions. This dinoflagellate can produce various paralytic shellfish toxins with concentrations ranging from 2.9 to 50.3 fmol/cell. The toxin profile was characterized by carbamate toxins (GTX3, GTX4 and GTX5) and N-sulfocarbamoyl toxins (C1, C2, C3 and C4). C2 dominated at 12-18 degrees C, but only for salinities ranging from 10 to 25 psu, whereas GTX5 became dominant at temperatures ranging from 21 to 30 degrees C at almost all salinities. There was no significant variation in the cellular toxin amount from 18 degrees C... |
| Tipo: Text |
Palavras-chave: Alexandrium catenella; PSP toxins; Temperature; Salinity; Light. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25969/24056.pdf |
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| Abadie, Eric; Zouher, Amzil; Belin, Catherine; Comps, Marie-annick; Elziere-papayanni, Panayota; Lassus, Patrick; Le Bec, Claude; Marcaillou-le Baut, Claire; Nezan, Elizabeth; Poggi, Robert. |
| A bloom of the toxic dinoflagellate Alexandrium tamarense was observed for the first time in Thau iagoon between November and December 1998. Maximum cell concentrations detected in shellfish farming areas (85 000 Cells/1) were high enough to make mussels and clams unsuitable for human consumption (max. 850 ug. STX eq.100 g'1) while toxic levels in oysters did not exceed, or even reach, the international public health threshold established for PSP toxins. This event, new to French Mediterranean waters, was investigated by both local and national veterinary services and by the Ifremer teams involved in the National Monitoring Network for Phytoplankton and Phycotoxines (Réphy). The chronological and operational description of the event was compared with data... |
| Tipo: Text |
Palavras-chave: Alexandrium; Monitoring; Toxic dinoflagellates; Thau lagoon; Alexandrium; Surveillance; Dinoflagellés toxiques; Etang de Thau. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/rapport-884.pdf |
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| Masseret, Estelle; Banack, Sandra; Boumediene, Farid; Abadie, Eric; Brient, Luc; Pernet, Fabrice; Juntas-morales, Raoul; Pageot, Nicolas; Metcalf, James; Cox, Paul; Camu, William. |
| Background Dietary exposure to the cyanotoxin BMAA is suspected to be the cause of amyotrophic lateral sclerosis in the Western Pacific Islands. In Europe and North America, this toxin has been identified in the marine environment of amyotrophic lateral sclerosis clusters but, to date, only few dietary exposures have been described. Objectives We aimed at identifying cluster(s) of amyotrophic lateral sclerosis in the Hérault district, a coastal district from Southern France, and to search, in the identified area(s), for the existence of a potential dietary source of BMAA. Methods A spatio-temporal cluster analysis was performed in the district, considering all incident amyotrophic lateral sclerosis cases identified from 1994 to 2009 by our expert... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00168/27941/26234.pdf |
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| Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine. |
| Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French... |
| Tipo: Text |
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf |
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| Delzor, Aurelie; Couratier, Philippe; Boumediene, Farid; Nicol, Marie; Druet-cabanac, Michel; Paraf, Francois; Mejean, Annick; Ploux, Olivier; Leleu, Jean-philippe; Brient, Luc; Lengronne, Marion; Pichon, Valerie; Combes, Audrey; El Abdellaoui, Saida; Bonneterre, Vincent; Lagrange, Emmeline; Besson, Gerard; Bicout, Dominique J.; Boutonnat, Jean; Camu, William; Pageot, Nicolas; Juntas-morales, Raul; Rigau, Valerie; Masseret, Estelle; Abadie, Eric; Preux, Pierre-marie; Marin, Benoit. |
| Introduction: Amyotrophic lateral sclerosis (ALS) is the most common motor neurone disease. It occurs in two forms: (1) familial cases, for which several genes have been identified and (2) sporadic cases, for which various hypotheses have been formulated. Notably, the beta-N-methylamino-L-alanine (L-BMAA) toxin has been postulated to be involved in the occurrence of sporadic ALS. The objective of the French BMAALS programme is to study the putative link between L-BMAA and ALS. Methods and analysis: The programme covers the period from 1 January 2003 to 31 December 2011. Using multiple sources of ascertainment, all the incident ALS cases diagnosed during this period in the area under study (10 counties spread over three French regions) were collected.... |
| Tipo: Text |
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| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00443/55431/56952.pdf |
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| Registros recuperados: 38 | |
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