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Registros recuperados: 14
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Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp. BABT
Coelho,Irene da Silva; Queiroz,Marisa Vieira de; Costa,Maurício Dutra; Kasuya,Maria Catarina Megumi; Araújo,Elza Fernandes de.
The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regeneration was achieved (8.5 %); 80.0 % of protoplasts were nucleated, and 20.0 % anucleated. For Ceratorhiza sp., the largest number of protoplasts (4.0 × 10(7) protoplasts/mL) was achieved in 0.6 M NaCl, when 15 mg/mL of Lysing Enzymes and 15mg/mL of Glucanex, with 2-day-old fungal mycelium were used. The highest frequency of regeneration was 6.7 % using 0.5 M sucrose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ceratorhiza; Epulorhiza; Molecular biology; Protoplast; Regeneration.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100019
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Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao BABT
Lopes,Francis Julio Fagundes; Queiroz,Marisa Vieira de; Lima,Juliana Oliveira; Silva,Viviane Aline Oliveira; Araújo,Elza Fernandes de.
The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the influence of the promoter used to express the gene marker were also analyzed. The addition of BamHI or NotI increased the number of transformants by 3-10-fold and 3-fold, respectively, over the control without added enzyme. The use of pre-linearized plasmid did not increase the transformation efficiency in comparison with the circular plasmid. However, the frequency of multi-copy transformants increased significantly. The transformation procedure here reported resulted in better production of protoplasts...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Moniliophthora perniciosa; Transformation; Restriction enzyme.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100004
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Partial purification and characterization of xylanase produced by Penicillium expansum BABT
Querido,André Luiz de Souza; Coelho,Jorge Luiz Cavalcante; Araújo,Elza Fernandes de; Chaves-Alves,Virgínia Maria.
An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. The enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. The protein eluation profile showed only one form of xylanase that was partially characterized. The activity of purified xylanase was optimal at pH 5.5 and 40 ºC. The enzyme was stable at pH between 5.5 and 6.5 and temperatures between 20-40 ºC. The enzyme showed a Km of 3.03 mM and Vmax of 0.027 mumol min-1 mug -1 of protein. The enzymatic activity was increased 31 % by Mg2+ and 28 % by Al3+.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Partial purification; Xylan/xylanases; Penicillium expansum.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400016
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Development of a transformation system for Penicillium brevicompactum based on the Fusarium oxysporum nitrate reductase gene BJM
Varavallo,Maurílio Antônio; Queiroz,Marisa Vieira de; Pereira,Jorge Fernando; Ribeiro,Ronney Adriano; Soares,Marcos Antônio; Ribeiro,João Batista; Araújo,Elza Fernandes de.
Penicillium brevicompactum is a filamentous fungus that presents a potential for industrial use due its efficient pectinase production. A heterologous transformation system was developed for P. brevicompactum based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. One mutant named 4457-18X was chosen for the transformation experiments with the pNH24 vector containing de Fusarium oxysporum nitrate reductase gene. A frequency of approximately 3 transformants/µg DNA was obtained using the circular vector pNH24. This frequency was multiplied about 10 fold using the linearized vector with the Xba I...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitrate reductase; Penicillium brevicompactum; Heterologous transformation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200015
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Fermentation of sweet whey by recombinant Escherichia coli K011 BJM
Leite,Amarildo Ricardo; Guimarães,Walter Vieira; Araújo,Elza Fernandes de; Silva,Daison Olzany.
The production of ethanol from sweet whey using the recombinant Escherichia coli KO11, in batch fermentation, was tested. The maximum ethanol yield was reached after 96h, representing only 38% of the theoretical yield. The supplementation of whey with components of LB broth increased the maximum yield to 96% in 72h. The addition of 0.5% yeast extract to whey resulted in maximum yield of 74% at 36h and it increased to over 100% when yeast extract and trace metals solution (Fe++, Mn++ and Zn++) were added.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Sweet whey; Ethanol.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300011
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Morphological and molecular differentiation of the pectinase producing fungi Penicillium expansum and Penicillium griseoroseum BJM
Cardoso,Patrícia Gomes; Queiroz,Marisa Vieira de; Pereira,Olinto Liparini; Araújo,Elza Fernandes de.
Two species from the genus Penicillium, Penicillium expansum and P. griseoroseum (Brasilian isolates) were characterized morphologic and molecularlly. Morphological variability was detected among isolates in regard to colony morphology and to conidia coloration. The molecular characterization was based on the RAPD markers, telomeric fingerprinting and ITS sequencing. A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. The only internal transcribed spacer region variation detected between the two species was the additional six initial nucleotides. The analysis by telomeric...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ribossomal DNA; Internal Transcribed Spacer; Subtelomeric Region; Penicillium spp.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100015
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Genetic diversity of indigenous common bean (Phaseolus vulgaris L.) rhizobia from the state of Minas Gerais, Brazil BJM
Torres,Adalgisa Ribeiro; Cursino,Luciana; Muro-Abad,Júpiter Israel; Gomes,Eliane Aparecida; Araújo,Elza Fernandes de; Hungria,Mariangela; Cassini,Sérvio Túlio Alves.
We characterized indigenous common bean rhizobia from five districts of the state of Minas Gerais, Brazil. The isolates were trapped by two common bean varieties, the Mineiro Precoce (Andean origin) and Ouro Negro (Mesoamerican origin). Analysis by BOX-PCR of selected isolates detected a high level of genetic diversity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: BOX-PCR; Phaseolus vulgaris; Rhizobium.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400016
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Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression BJM
Lima,Juliana Oliveira; Pereira,Jorge Fernando; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de.
Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay. Although PL production higher than the wild type has been...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-Deoxy-d-glucose; Ultraviolet mutagenesis; Pectinolytic complex; Spontaneous mutant.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300602
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Isolation and characterization of endophytic bacteria isolated from the leaves of the common bean (Phaseolus vulgaris) BJM
Costa,Leonardo Emanuel de Oliveira; Queiroz,Marisa Vieira de; Borges,Arnaldo Chaer; Moraes,Celia Alencar de; Araújo,Elza Fernandes de.
The common bean is one of the most important legumes in the human diet, but little is known about the endophytic bacteria associated with the leaves of this plant. The objective of this study was to characterize the culturable endophytic bacteria of common bean (Phaseolus vulgaris. leaves from three different cultivars (Vermelhinho, Talismã, and Ouro Negro) grown under the same field conditions. The density of endophytic populations varied from 4.5 x 10² to 2.8 x 10³ CFU g-1 of fresh weight. Of the 158 total isolates, 36.7% belonged to the Proteobacteria, 32.9% to Firmicutes, 29.7% to Actinobacteria, and 0.6% to Bacteroidetes. The three P. vulgaris cultivars showed class distribution differences among Actinobacteria, Alphaproteobacteria and Bacilli. Based...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Common bean; Phaseolus vulgaris; 16S rDNA; Diversity indices.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400041
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Isolation of recombinant strains with enhanced pectinase production by protoplast fusion between Penicillium expansum and Penicillium griseoroseum BJM
Varavallo,Maurilio Antonio; Queiroz,Marisa Vieira de; Lana,Taís Guimarães; Brito,Admilson Toscano Ribeiro de; Gonçalves,Daniel Bonoto; Araújo,Elza Fernandes de.
Protoplast fusion between complementary auxotrophic and morphological mutant strains of Penicillium griseoroseum and P. expansum was induced by polyethylene glycol and calcium ions (Ca2+). Fusant strains were obtained in minimal medium and a prototrophic strain, possibly diploid, was chosen for haplodization with the fungicide benomyl. Different recombinant strains were isolated and characterized for occurrence of auxotrophic mutations and pectinolytic enzyme production. The fusant prototrophic did not present higher pectinase production than the parental strains, but among 29 recombinants analyzed, four presented enhanced enzyme activities. The recombinant RGE27, which possesses the same auxotrophic and morphologic mutations as the P. griseoroseum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protoplast fusion; Pectinases; Penicillium.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100011
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Método para mapeamento de locos controladores de características oligogênicas Ciência Rural
Rocha,Rodrigo Barros; Barros,Willian Silva; Muro-Abad,Júpiter Israel; Tomaz,Rafael Simões; Cruz,Cosme Damião; Barros,Everaldo Gonçalves de; Araújo,Elza Fernandes de.
Características oligogênicas de distribuição discreta e expressão governada por poucos genes de maior efeito têm se mostrado importantes na condução dos programas de melhoramento, com destaque para a resposta de resistência das plantas às doenças. Métodos tradicionais de detecção de QTL's, que pressupõem normalidade e herança governada por múltiplos fatores, não deveriam ser utilizados para mapeamento dessas características de distribuição discreta e interação epistática predominante. O objetivo deste trabalho é avaliar os resultados de um método para mapeamento e detecção de locos controladores da expressão de características oligogênicas, OTL's (Oligogenic Trait Loci). Esse método, definido como MMCO (Método de Mapeamento de Características...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mapeamento genético; Locos controladores de características oligênicas (OTL's).
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200012
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Characterization of the omlA gene from different serotypes of Actinobacillus pleuropneumoniae: a new insight into an old approach Genet. Mol. Biol.
Rossi,Ciro César; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de; Bazzolli,Denise Mara Soares.
The OmlA protein is a virulence factor of Actinobacillus pleuropneumoniae, an important pathogen in pigs. The polymorphisms present in the omlA gene sequence of 15 reference serotypes of A. pleuropneumoniae and non-serotypable isolates were assessed to determine the possible evolutionary relationship among them and to validate the importance of this gene as a molecular marker for the characterization of this bacterium. Divergence among the 15 serotypes of A. pleuropneumoniae probably resulted initially from two major evolutionary events that led to subsequent differentiation into nine groups. This differentiation makes it possible to characterize most of the serotypes by using bionformatics, thereby avoiding problems with immunological cross-reactivity. A...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actinobacillus pleuropneumoniae; OmlA gene; Phylogenetic reconstruction; Porcine pleuropneumonia.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200015
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Detection and mapping of a lethal locus in a eucalyptus hybrid population PAB
Rosado,Tatiana Barbosa; Tomaz,Rafael Simões; Rocha,Rodrigo Barros; Rosado,Antônio Marcos; Alves,Alexandre Alonso; Araújo,Elza Fernandes de; Alfenas,Acelino Couto; Cruz,Cosme Damião.
The objective of this work was to verify the existence of a lethal locus in a eucalyptus hybrid population, and to quantify the segregation distortion in the linkage group 3 of the Eucalyptus genome. A E. grandis x E. urophylla hybrid population, which segregates for rust resistance, was genotyped with 19 microsatellite markers belonging to linkage group 3 of the Eucalyptus genome. To quantify the segregation distortion, maximum likelihood (ML) models, specific to outbreeding populations, were used. These models consider the observed marker genotypes and the lethal locus viability as parameters. The ML solutions were obtained using the expectation‑maximization algorithm. A lethal locus in the linkage group 3 was verified and mapped, with high confidence,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eucalyptus; Gametic selection; Genetic mapping; QTL; Rust resistance; Segregation distortion.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000900008
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Morphological and molecular characterization of Pisolithus in soil under eucalyptus plantations in Brazil Rev. Bras. Ciênc. Solo
Kasuya,Maria Catarina Megumi; Coelho,Irene da Silva; Campos,Daniela Tiago da Silva; Araújo,Elza Fernandes de; Tamai,Yutaka; Miyamoto,Toshizumi.
Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained by phylogenetic relationship based on internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of these isolates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ectomycorrhizal fungus; Phylogenetic analysis; Internal transcribed spacer (ITS); Nuclear ribosomal DNA; Taxonomy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000600013
Registros recuperados: 14
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