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| Registros recuperados: 59 | |
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| Thouard, Emmanuel; Buchet, Vincent; Cahu, Chantal; Coeurdacier, Jean-luc; Elie, Pierre; Gerard, André; Hussenot, Jerome; Legendre, Marc; Prou, Jean; Robert, Rene. |
| Les missions réalisées fin 2004 et début 2005 ont permis de faire le point sur les coopérations potentielles entre l'INSTM et l'IFREMER. Un certain nombre de domaines de coopération et de sujets de collaboration ont été identifiés et se sont traduits (ou se traduiront) par des actions de coopération (missions, stages, formations) soutenues financièrement par le SCAC de l'Ambassade de France à Tunis. La coopération entre l'IFREMER et l'INSTM dans le domaine de l'aquaculture a été très active dans le cadre du programme« Aquaculture 2001 » (1997-2001). Depuis cette période les relations se sont limitées à la préparation du programme DORAD qui était la suite d' «Aquaculture 2001 » . En l'absence du financement FSP sollicité pour DORAD, les deux organismes ont... |
| Tipo: Text |
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| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00305/41648/40857.pdf |
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| Villeneuve, Laure; Gisbert, Enric; Moriceau, Jacques; Cahu, Chantal; Zambonino, Jose-luis. |
| The effect of the feeding period on larval development was investigated in European sea bass larvae by considering the expression level of some genes involved in morphogenesis. Larvae were fed a control diet except during three different periods (period A: from 8 to 13 d post-hatching (dph); period B: from 13 to 18 dph; period C: from 18 to 23 dph) with two compound diets containing high levels of vitamin A or PUFA. European sea bass morphogenesis was affected by these two dietary nutrients during the early stages of development. The genes involved in morphogenesis could be modulated between 8 and 13 dph, and our results indicated that retinoids and fatty acids influenced two different molecular pathways that in turn implicated two different gene cascades,... |
| Tipo: Text |
Palavras-chave: Retinoid pathway; Morphogenesis; Vitamin A; PUFA; Sea bass larvae. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1215.pdf |
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| Tovar-ramirez, D.; Mazurais, David; Gatesoupe, J. F.; Quazuguel, Patrick; Cahu, Chantal; Zambonino-infante, J. L.. |
| The main goal of this work was to determine the effect of dietary live yeast Debaryomyces hansenii on the enzymatic antioxidative status of sea bass Dicentrarchus labrax larvae. Growth, activity and expression of the main antioxidative enzymes: catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD), and heat shock protein (HSP70) were measured in sea bass larvae at 23 and 48 days after hatching. Larvae were fed on two microdiets: group one, fed microdiet containing live yeast and the control group fed microdiet without yeast. Heat shock protein 70 showed the same expression levels in both fish larvae fed yeast and the control diet. The group fed D. hansenii showed highest growth and lower activity and expression levels of GPX and SOD... |
| Tipo: Text |
Palavras-chave: Antioxidant enzymes; Probiotics; Debaryomyces hansenii; Dicentrarchus labrax. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11299/7881.pdf |
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| Cahu, Chantal; Zambonino, Jose-luis; Corraze, Genevieve; Coves, Denis. |
| Triplicate groups of juvenile seabass (initial weight of 241 g) were fed during 13 weeks three isonitrogenous experimental diets containing different lipid levels, 12% (LL group), 21% (ML group) and 30% (HL group). At the end of the experiment, fish weight gain was similar among the 3 dietary groups. Intestinal brush border membranes were purified for each dietary group; one part of the brush border fraction was dedicated to enzyme assays, the remaining fraction being used for lipid extraction followed by fatty acid analysis. The fatty acid composition of the brush border membrane differed among the 3 groups, although the 3 experimental diets had the same fatty acid composition. The increase in dietary lipid level resulted in a lowering in (n-3)... |
| Tipo: Text |
Palavras-chave: Membrane lipid; Intestine; Fish; Fatty acids; Dietary lipids; Brush border hydrolases. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-901.pdf |
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| Gamboa Delgado, Julián; Molina Poveda, César; Cahu, Chantal. |
| A study was conducted to evaluate variations of digestive enzyme activities in Litopenaeus vannamei (Boone) reared in commercial ponds under semi-intensive conditions. Shrimp were collected at each body weight increase of 2 g. As the shrimp grew (2-12 g), significant increases in the activities of lipase and chymotrypsin were observed. The total protease activity decreased from 6 g onwards. Trypsin activity showed a peak at 6 g and amylase activity increased two-fold after 2 g. Additionally, the stomach contents were analysed microscopically for shrimp between 2 and 10 g. Plant matter contributed above 30% of the total stomach content in 6-, 8- and 10-g shrimp. Detritus represented 58% and 62% of the total stomach content in 2- and 4-g shrimp,... |
| Tipo: Text |
Palavras-chave: Semi intensive culture; Stomach content; Digestive enzymes; Litopenaeus vannamei; Shrimp. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-902.pdf |
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| Coves, Denis; De Vogue, Benoist; Desbruyeres, Elisabeth; Dhormes, Benoit; Fievet, Julie; Huelvan, Christine; Lallement, Stephane; Le Gall, Marie-madeleine; Ruelle, Francois; Vidal, Marie-odile; Castex, M.; Mazurais, David; Cahu, Chantal; Gatesoupe, François-joel. |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16189/13702.pdf |
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| Cahu, Chantal. |
| The aim of this paper is to evaluate to what extent, the study of feeding regimes of fish could be valuable to indicate the possibilities and the constraints of their domestication. Though the natural feeding regime of fish seems to be disadvantageous for their domestication, as protein requirement is high, different species could be considered as domesticated. These species are herbivorous, detritivorous, omnivorous as well as carnivorous. In the same way, there are large differences in gut anatomy, but domestication concerns species with or without stomach, and species with short or very long intestine. Nutritional requirements do not allow predicting species that could be domesticated. Some species seem to be able to use vegetable protein in... |
| Tipo: Text |
Palavras-chave: TROUT ONCORHYNCHUS MYKISS; TURBOT PSETTA MAXIMA; RAINBOW TROUT; TOTAL REPLACEMENT; FLESH QUALITY; LIVE FOOD; DIET; PROTEIN; GROWTH; LARVAE. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00000/11021/7571.pdf |
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| Cahu, Chantal; Ronnestad, I; Grangier, V; Zambonino, Jose-luis. |
| In order to assess the influence of dietary protein on digestive enzyme expression and cholecystokinin (CCK) content in sea bass larvae, four groups of larvae were fed experimental diets from mouth opening until day 42: three isonitrogenous diets with increasing protein hydrolysate levels (0%, 14% and 46% of crude matter) and one diet incorporating starch. The groups fed high starch or high protein hydrolysate level exhibited the lowest growth. The final weight in these groups was 9.5 and 5.6 mg, respectively, whereas it reached approximately 20.0 mg in the groups fed 0% or 14% protein hydrolysate level. The highest levels of trypsin secretion were observed in larvae fed the lowest protein hydrolysate level. Paradoxically, the groups fed diet containing... |
| Tipo: Text |
Palavras-chave: Starch; Sea bass larvae; Protein hydrolysate; Pancreatic enzyme secretion; Cholecystokinin. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-442.pdf |
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| Santigosa, Ester; Geay, Florian; Tonon, Thierry; Le Delliou, Herve; Kuhl, Heiner; Reinhardt, Richard; Corcos, Laurent; Cahu, Chantal; Zambonino, Jose-luis; Mazurais, David. |
| Fish are the main source of the n-3 highly unsaturated fatty acids, which are crucial for human health. Their synthesis from C18 precursors is mediated by desaturases and elongases, but the activity of these enzymes has not been conclusively established in marine fish species. This study reports the cloning, tissue expression, and functional characterization of a sea bass (Dicentrarchus labrax L.) Δ6-desaturase and one of its splicing variants. Two cDNAs with open reading frames of 1,346 and 1,354 bp were cloned and named D6D and D6D-V, respectively. Both deduced protein sequences (445 and 387 amino acids, respectively) contained two transmembrane regions and the N-terminal cytochrome b5 domain with the HPGG motif characteristic of microsomal desaturases.... |
| Tipo: Text |
Palavras-chave: Sea bass (Dicentrarchus labrax); Desaturase; HUFA biosynthesis; Fish; EPA. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00023/13470/10477.pdf |
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| Castro-ruiz, Diana; Andree, Karl B.; Solovyev, Mikhail M.; Fernández-méndez, Christian; García-dávila, Carmen; Cahu, Chantal; Gisbert, Enric; Darias, Maria J.. |
| Pseudoplatystoma punctifer is an Amazonian catfish highly appreciated for its high flesh quality, size, and commercial value. Its aquaculture is pursued to satisfy the demands of an increasing population in the region. However, knowledge of the nutritional needs during the early life stages is necessary for improving growth and reducing the incidence of cannibalism, factors that limit the success of its commercial farming. This study aimed at evaluating the influence of four diets containing different protein and lipid levels (30:15, 30:10, 45:15, or 45:10 in %) in the digestive physiology and performance of early juveniles. The results showed that the dietary protein:lipid as well as carbohydrate levels and ratios influenced differently the whole-body... |
| Tipo: Text |
Palavras-chave: Neotropical fish; Aquaculture; Digestive enzymes; Gene expression; Nutrition; Juveniles; Histology; Development; Macronutrients; Diet. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00678/79019/81420.pdf |
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| Zambonino, Jose-luis; Cahu, Chantal. |
| During these last 20 years, many studies have focussed on the development of the digestive tract in marine fish larvae. Most of the studies aimed at acquiring knowledge on the optimal form of dietary supply for different nutrients, in order to formulate a compound diet able to totally replace live preys in the fish larvae feeding sequence. Consequently, most of the studies aimed at describing the effect of dietary adaptation on digestive enzymes profile, the morphology of the main organs, while others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport...) were poorly investigated. This review reports the more recent data on dietary modulation of digestive enzymes with a particular emphasis, when possible, on the... |
| Tipo: Text |
Palavras-chave: Nuclear receptors; Digestive enzymes; Fatty acids; Vitamins; Dietary proteins and lipids; Fish larvae. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3045.pdf |
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| Cahu, Chantal; Gisbert, Enric; Villeneuve, Laure A. N.; Morais, Sofia; Hamza, Neila; Wold, Per-avid; Zambonino, Jose-luis. |
| The aim of this paper is to provide explanations of how dietary phospholipid (PL) globally improves fish larval development, including growth and survival, digestive functions and skeletal development, and to propose optimal PL levels and sources in fish larval diets. Dietary incorporation of 8-12% PL related to dry matter (d.m.) promotes growth and enhanced survival in various species. Marine source PL, incorporating highly unsaturated fatty acids, was most efficient than soybean lecithin. This beneficial effect was explained by an enhancement in digestive functions, assessed by digestive enzyme activities and histomorphology. Nevertheless, 1.5-2.5% highly unsaturated fatty acids related to diet d.m. supplied by PL improved growth, survival and skeletal... |
| Tipo: Text |
Palavras-chave: Gene regulation; Skeletal development; Digestive functions; Fish larvae; Phospholipid. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6460.pdf |
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| Zouiten, Dora; Ben Khemis, Ines; Masmoudi, Ahmed Slaheddin; Huelvan, Christine; Cahu, Chantal. |
| The quality of development in intensive or mesocosm hatchery-reared Dicentrarchus labrax larvae was investigated using physiological indicators assessing ontogeny. Larvae were reared in intensive (120 L tanks) and in mesocosm systems (20 m(3) enclosures) with the same feeding sequence, excluding the wild zooplankton naturally available in mesocosms. Faster growth was recorded since early development [16 day after hatching (DAH)] in the mesocosm. Maturation of the digestive system also occurred earlier as indicated by the higher amylase secretion ratios, the intestinal maturation index (alkaline phosphatase/leucine-alanine peptidase and aminopeptidase-N/leucine-alanine peptidase ratios) and the more developed intestinal epithelium at 23 DAH. Nevertheless,... |
| Tipo: Text |
Palavras-chave: Fish larvae; Hatchery; Mesocosm; Ontogenesis; Enzyme; Skeletogenesis. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16225/14064.pdf |
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| Castro-ruiz, Diana; Mozanzadeh, Mansour Torfi; Fernández-méndez, Christian; Andree, Karl B.; García-dávila, Carmen; Cahu, Chantal; Gisbert, Enric; Darias, Maria J.. |
| The aim of the study was to evaluate the functional ontogeny of the digestive system of Pseudoplatystoma punctifer through the analysis of the activity of the main intestinal (alkaline phosphatase, aminopeptidase N, maltase and leucine-alanine peptidase), pancreatic (trypsin, chymotrypsin, total alkaline proteases, bile-salt activated lipase and amylase) and gastric (pepsin) enzymes. Larvae were raised in triplicate in a recirculation system from 4 to 27 days post fertilization (dpf) at an initial density of 90 larvae L−1, 27.8 ± 0.7 °C and 0 L: 24D photoperiod. Larvae were fed from 4 to 17 dpf with Artemia nauplii and weaned onto an experimentally formulated feed (crude protein content ~ 45%; crude fat content ~ 10%; crude carbohydrate ~ 8%) within... |
| Tipo: Text |
Palavras-chave: Pseudoplatystoma punctifer; Ontogeny; Enzymatic activity; Digestive system; Brush border; Pancreatic enzymes; Gastric enzymes. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00479/59082/61723.pdf |
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| Ma, Hongming; Cahu, Chantal; Zambonino, Jose-luis; Yu, Hairui; Duan, Qingyuan; Le Gall, Marie-madeleine; Mai, Kangsen. |
| Large yellow croaker is an important marine fish species for aquaculture in China. Larval rearing of this species is still based on live prey feeding. The aim of this study was to describe the onset and development of the main digestive enzymes during larval development of this species, in order to obtain essential data for the formulation of a compound diet adapted to larvae. Enzymatic assays were conducted from day I post hatching to day 40 in larvae fed the following live prey sequence: rotifers, Artemia nauplii, copepods, and then frozen copepods. Assays were done on whole larvae until day 19, then on dissected larvae and on purified brush border membranes of intestine. Pancreatic enzymes, amylase, and trypsin, showed high activities from early stages,... |
| Tipo: Text |
Palavras-chave: Trypsin; Pseudosciaena crocea; Fish larval development; Amylase; Aminopeptidase N; Alkaline phosphatase. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-444.pdf |
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| Morais, Sofia; Conceicao, L; Ronnestad, I; Koven, W; Cahu, Chantal; Zambonino, Jose-luis; Dinis, M. |
| A growth depressing effect of high dietary neutral lipid levels in marine fish larvae has been reported. This may be a result of a decrease in the efficiency or activity of digestive enzymes, a reduction in absorption efficiency and/or a decrease in food intake. The present work reviews recent studies carried out on commercially valuable species (Atlantic herring, Senegalese sole, European seabass and gilthead seabream) that investigated the effects of neutral lipid level and lipid source (fatty acid composition) on some of these key factors influencing larval growth. The results seem to collectively indicate that lipid transport from the enterocytes into the body may be more problematic in larval stages dealing with high neutral lipid diets than lipolytic... |
| Tipo: Text |
Palavras-chave: Ingestion; Lipid droplets; Digestion; Absorption; Fatty acid composition; Lipid quantity. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3029.pdf |
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| Zambonino, Jose-luis; Gisbert, E; Sarasquete, C; Navarro, I; Gutiérrez, J; Cahu, Chantal. |
| The ontogeny of the digestive tract of marine fish larvae has been the subject of many studies these last twenty years. Beyond the scientific interest for such animal species, particularly for developmental aspects, most of these studies aimed to come up with the expectations of commercial hatcheries by reducing the bottlenecks in larvae culture and weaning processes (switch from live preys to compound diet feeding sequence). Consequently, the profile and dietary adaptation of digestive enzymes, the description and histology of the main organs have been extensively studied, when others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport…) were poorly investigated and this imbalanced knowledge is showed through this... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/00086/19684/17307.pdf |
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| Ducasse Cabanot, S; Zambonino, Jose-luis; Richard, N; Medale, Francoise; Corraze, Genevieve; Mambrini, M; Robin, Jean; Cahu, Chantal; Kaushik, Sadasivam; Panserat, S. |
| For sustainable aquaculture, the removal of marine resource ingredients in fish diets is an important objective. While most studies focus on the replacement of fish oil by vegetable oil, little is known on the nutritional effects of presence (which corresponds to the control diet) or absence of dietary fish oil. We studied fatty acid composition of brush-border membranes and digestive enzyme activities of the intestine and measured the expression and activities of several enzymes involved in the hepatic intermediary metabolism of rainbow trout (Oncorhynchus mykiss) fed for 7 weeks with or without fish oil. The diets were pair-fed to ensure that fish fed either diet had comparable carbohydrate and protein intakes. Absence of fish oil significantly reduced... |
| Tipo: Text |
Palavras-chave: Rainbow trout; Metabolism; Fish oil; Digestion; Aquaculture. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3506.pdf |
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| Darias, M. J.; Wing, O. Lan Chow; Cahu, Chantal; Zambonino, Jose-luis; Mazurais, David. |
| P>The alcian blue-alizarin red technique was successfully adjusted to stain developing European sea bass (Dicentrarchus labrax) larvae. For an optimal staining protocol design both larval size and their morphological characteristics at each developmental stage were considered, since such parameters notably influence the staining of tissues. The incubation times of the different solutions were adjusted to allow the stain penetration for revealing the integrity of cartilaginous and bony tissues without significant tissue degradation. Three developmental windows were determined for an optimal staining procedure: (i) 4.5-6.4 mm, (ii) 6.7-8.7 mm, and (iii) 12.8-15.5 mm total length (TL). In order to validate the continuity of staining along the larval... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11371/8364.pdf |
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| Registros recuperados: 59 | |
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