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José,Anderson C.; Ligterink,Wilco; Davide,Antonio Claudio; Silva,Edvaldo A. Amaral da; Hilhorst,Henk W.M.. |
Seeds of Magnolia ovata were dried to different water contents to assess the viability and transcript abundance of genes related to seed development, cell cycle, cytoskeleton and desiccation tolerance.The expression of development, cell cycle and cytoskeleton relative genes (ABI3, CDC2-like and ACT2) alone could not explain the germination behaviour of M. ovata seeds in relation to drying damage. Irrespective of their initial water content, the seeds performed in the same way during the initial period of germination and the deleterious effects of desiccation only occurred in later stages. Expression of PKABA1, sHSP17.5 and 2-Cys-PRX did not show a relationship with desiccation. However, the expression patterns of PKABA1 and sHSP17.5 suggested the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Desiccation tolerance; Drying; Gene expression; Magnolia ovata. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222009000100030 |
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Faria,José M.R.; Davide,Lisete C.; Silva,Edvaldo A. A. da; Davide,Antonio C.; Pereira,Roselaine C.; van Lammeren,André A.M.; Hilhorst,Henk W.M.. |
The remarkably short storability of recalcitrant seeds has prevented their inclusion in programs of ex situ conservation. The causes of their desiccation sensitivity and rapid decline in viability during storage are not fully elucidated yet. In this study the highly recalcitrant, fully viable embryos of the tree species Inga vera subsp. affinis were stored under various conditions and analyzed physiologically and cytologically at intervals, in order to obtain more insights in their loss of viability during storage. Embryos stored fully hydrated at 5ºC fully lost viability in 18 d. Sealed storage of partially dehydrated embryos slightly prolonged their viability, with 95% germination being attained after 14 d of storage. However, after 30 d of storage,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abscisic acid; Polyethylene glycol; Recalcitrant seeds; Seed conservation. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000400008 |
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