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Van Wambeke, France; Pulido, Elvira; Catala, Philippe; Dinasquet, Julie; Djaoudi, Kahina; Engel, Anja; Garel, Marc; Guasco, Sophie; Barbara, Marie; Nunige, Sandra; Taillandier, Vincent; Zancker, Birthe; Tamburini, Christian. |
Ectoenzymatic activity, prokaryotic heterotrophic abundances and production were determined in the Mediterranean Sea. Sampling was carried out in the sub-surface, the deep chlorophyll maximum layer (DCM), the core of the Levantine intermediate waters and in the deeper part of the mesopelagic layers. Michaelis–Menten kinetics were assessed using a large range of concentrations of fluorogenic substrates (0.025 to 50 µM). As a consequence, Km (Michaelis–Menten half-saturation constant) and Vm (maximum hydrolysis velocity) parameters were determined for both low- and high-affinity enzymes for alkaline phosphatase, aminopeptidase (LAP) and β-glucosidase (βGLU). Based on the constant derived from the high-LAP-affinity enzyme (0.025–1 µM substrate concentration... |
Tipo: Text |
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Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00642/75452/76268.pdf |
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Djaoudi, Kahina; Van Wambeke, France; Barani, Aude; Bhairy, Nagib; Chevaillier, Servanne; Desboeufs, Karine; Nunige, Sandra; Labiadh, Mohamed; Des Tureaux, Thierry Henry; Lefevre, Dominique; Nouara, Amel; Panagiotopoulos, Christos; Tedetti, Marc; Pulido-villena, Elvira. |
The surface ocean receives important amounts of organic carbon from atmospheric deposition. The degree of bioavailability of this source of organic carbon will determine its impact on the marine carbon cycle. In this study, the potential availability of dissolved organic carbon (DOC) leached from both desert dust and anthropogenic aerosols to marine heterotrophic bacteria was investigated. The experimental design was based on 16 d incubations, in the dark, of a marine bacterial inoculum into artificial seawater amended with water-soluble Saharan dust (D treatment) and anthropogenic (A treatment) aerosols, so that the initial DOC concentration was similar between treatments. Glucose-amended (G) and non-amended (control) treatments were run in parallel. Over... |
Tipo: Text |
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Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00677/78941/81326.pdf |
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Gazeau, Frédéric; Ridame, Céline; Van Wambeke, France; Alliouane, Samir; Stolpe, Christian; Irisson, Jean-olivier; Marro, Sophie; Grisoni, Jean-michel; De Liège, Guillaume; Nunige, Sandra; Djaoudi, Kahina; Pulido-villena, Elvira; Dinasquet, Julie; Obernosterer, Ingrid; Catala, Philippe; Guieu, Cécile. |
In Low Nutrient Low Chlorophyll areas, such as the Mediterranean Sea, atmospheric fluxes represent a considerable external source of nutrients likely supporting primary production especially during stratification periods. These areas are expected to expand in the future due to lower nutrient supply from sub-surface waters caused by enhanced stratification, likely further increasing the role of atmospheric deposition as a source of new nutrients to surface waters. Yet, whether plankton communities will react differently to dust deposition in a warmer and acidified environment remains an open question. The impact of dust deposition both in present and future climate conditions was assessed through three perturbation experiments in the open Mediterranean Sea.... |
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Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00688/80015/82994.pdf |
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