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Population-specific variations of the genetic architecture of sex determination in wild European sea bass Dicentrarchus labrax L. ArchiMer
Faggion, Sara; Vandeputte, Marc; Chatain, Beatrice; Gagnaire, Pierre-alexandre; Allal, Francois.
Polygenic sex determination (PSD) may show variations in terms of genetic and environmental components between populations of fish species exposed/adapted to different environments. The European sea bass (Dicentrarchus labrax) is an interesting model, combining both a PSD system and a genetic subdivision into an Atlantic and a Mediterranean lineage, with genetic substructures within the Mediterranean Sea. Here, we produced experimental progeny crosses (N = 927) from broodstock sampled in four wild populations (North Atlantic, NAT; Western Mediterranean, WEM; North-Eastern Mediterranean, NEM; South-Eastern Mediterranean, SEM). We found less females than males in the progeny, both in the global dataset (32.5%) and within each paternal group (from 25.1% for...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00464/57609/59864.pdf
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Response to selection for growth in an interspecific hybrid between Oreochromis mossambicus and O. niloticus in two distinct environments ArchiMer
De Verdal, Hugues; Rosario, Westly; Vandeputte, Marc; Muyalde, Nerafe; Morissens, Pierre; Baroiller, Jean-francois; Chevassus, Bernard.
The development of a saline tolerant tilapia strain able to grow fast is of importance in the Philippines, where 240 000 ha of brackish water ponds are available. To this end, founder hybridization between Oreochromis niloticus (with favorable growth traits) and O. mossambicus (with favorable salinity tolerance traits) was performed and followed by backcrossing with O. mossambicus to develop a strain highly tolerant to saline environments. Genetic selection for growth performance was subsequently conducted. The aim of the present study was to estimate growth performance and survival of the hybrid in two rearing environments following four generations of genetic selection. A comparison between the hybrid and an internal reference line was performed in two...
Tipo: Text Palavras-chave: Salinity tolerance; Selection; Hybridization; Genetic gain; Heritability; Survival rate.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00187/29836/28287.pdf
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How to genetically increase fillet yield in fish: Relevant genetic parameters and methods to predict genetic gain ArchiMer
Vandeputte, Marc; Fraslin, Clemence; Haffray, Pierrick; Bestin, Anastasia; Allal, Francois; Kocour, Martin; Prchal, Martin; Dupont-nivet, Mathilde.
Fillet yield (i.e. the proportion of edible muscle in a fish) is a key economic trait for species sold as fillets. Its genetic improvement is complicated by several of its characteristics 1) it is a ratio trait, 2) its numerator (fillet weight) and denominator (body weight) are strongly correlated (correlations in the range 0.89-0.99), 3) it offers little phenotypic variation and 4) it cannot be measured on alive breeding candidates. In a former study, we showed that it could be improved by selection, especially with three selection indices, fillet yield, residual fillet weight and a ratio-specific linear index. However, it is well known that the heritability of ratio traits does not permit a reliable prediction of genetic gains. As predictability of...
Tipo: Text Palavras-chave: Aquaculture; Genetics; Heritability; Selection; Linear index; Residual fillet weight; Selection response; Simulation; Processing yields.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00612/72378/71390.pdf
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Agonistic behaviour and feed efficiency in juvenile Nile tilapia Oreochromis niloticus ArchiMer
De Verdal, Hugues; O'Connell, Catherine Maeve; Mekkawy, Wagdy; Vandeputte, Marc; Chatain, Beatrice; Bégout, Marie-laure; Benzie, John A.h..
Given the strong effects of behavioural hierarchies on growth in many cultured species and the key role of feed efficiency in aquaculture economics, understanding the nature of the interaction of these variables is important for the sustainability of aquaculture. The relationship between agonistic behaviour, growth and feed efficiency in Nile tilapia, Oreochromis niloticus, was studied by rearing 120 fish in eight aquaria. Fish were video-recorded to estimate the occurrence of agonistic behaviour during a fasting and a refeeding period. Growth, feed intake and feed conversion efficiency (FCE - calculated as the inverse of feed conversion ratio (FCR)) were subsequently measured individually for each fish. Fish showed 58% less agonistic traits during the...
Tipo: Text Palavras-chave: Agonstic behaviour; Feed efficiency; Nile tilapia; Performances; Correlation.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00483/59495/62363.pdf
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Morphological predictors of slaughter yields using 3D digitizer and their use in a common carp breeding program ArchiMer
Prchal, Martin; Kocour, Martin; Vandeputte, Marc; Kause, Antti; Vergnet, Alain; Zhao, Jinfeng; Gela, David; Kašpar, Vojtěch; Genestout, Lucie; Bestin, Anastasia; Haffray, Pierrick; Bugeon, Jérôme.
Slaughter yields are traits of high interest especially for fish species sold in processed form like headless carcass or fillets, as well as with regard to increasing consumer preference for easy-to-prepare fish products. However, slaughter yields cannot be measured on live fish and thus their genetic improvement through mass selection is impossible. The usual alternatives are sib selection and/or indirect selection on correlated traits or morphological predictors of slaughter yields. In the present study, we assessed the possibility of using a combination of 3D digitized landmarks and ultrasound measurements in genetic improvement of slaughter yields in common carp. DNA – pedigreed market-size carp (n = 1553 fish) were produced from a partial factorial...
Tipo: Text Palavras-chave: Fillet yields; 3D predictors; Genetic parameters; Indirect selection; Breeding program.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00604/71625/70070.pdf
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Genetic improvement of common carp (Cyprinus carpio L.) ArchiMer
Vandeputte, Marc.
Common carp was the first domesticated fish species and is now a major species in worldwide aquaculture. Although research has generated a considerable body of knowledge about its genetics, genetic improvement programmes are virtually non-existent in this species and only crossbreeding is used in practice. The main reasons are the inefficiency of methods usually effective in other aquaculture species (monosexing, triploidy), and the bad image of selective breeding which is difficult to implement in ponds, and for which reliable genetic parameters were missing until recently. These parameters are now becoming available through the use of parentage assignment with microsatellite markers. However, the question of selective breeding for growth remains far from...
Tipo: Text Palavras-chave: Selection; Population genetics; Cyprinus carpio; Breeding; Aquaculture.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6563.pdf
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Response to domestication and selection for growth in the European sea bass (Dicentrarchus labrax) in separate and mixed tanks ArchiMer
Vandeputte, Marc; Dupont Nivet, M; Haffray, P; Chavanne, H; Cenadelli, S; Parati, K; Vidal, Marie-odile; Vergnet, Alain; Chatain, Beatrice.
Selective breeding of European sea bass (Dicentrarchus labrax) receives a growing interest, as the estimated heritability of growth is medium to high. In this study, we compared the offspring of four groups of sea bass sires, mated with the same wild dams: wild (W), first generation of domestication (D), first generation of mass selection for length (M), first generation of PROSPER-like selection for length (P). The comparison was done both in replicated tanks (separate rearing) and in mixed tanks (mixed rearing) where sire origins were recovered by genotyping of eight microsatellite markers. Weight, length and growth rate were measured from day 238 post-fertilization (69 g mean weight) to day 611 post-fertilization (390 g mean weight). Both in mixed and...
Tipo: Text Palavras-chave: Nephrocalcinosis; Competition; Microsatellites; Parentage assignment; Realized heritability; Selection response; Growth; Selective breeding; Dicentrarchus labrax; European sea bass.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-5303.pdf
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Genetic relationship between koi herpesvirus disease resistance and production traits inferred from sibling performance in Amur mirror carp ArchiMer
Zhao, Jinfeng; Prchal, Martin; Palaiokostas, Christos; Houston, Ross D.; Kause, Antti; Vandeputte, Marc; Vergnet, Alain; Bugeon, Jérôme; Bestin, Anastasia; Veselý, Tomáš; Pokorová, Dagmar; Piačková, Veronika; Pojezdal, Lubomír; Genestout, Lucie; Gela, David; Kroupová, Hana K.; Kocour, Martin.
Koi herpesvirus disease (KHVD) is currently the most serious threat to global carp farming. Prevention is a sensible strategy for tackling this disease and improved genetic resistance of carp strains is a desirable breeding goal. To study the potential for multitrait selection, the objective of the current study was to estimate the genetic correlations between KHVD resistance and production traits in Amur mirror carp. A total of 1500 fingerlings from four factorial crosses of five dams and ten sires were challenged with Koi herpesvirus (KHV). Juvenile growth-related traits were collected on the same individuals before the challenge test. Production traits were measured on siblings of the challenged population at different life stages (yearling to market...
Tipo: Text Palavras-chave: Common carp; KHVD; Body shape traits; Slaughter yields; Heritability; Genetic correlation.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00604/71623/70066.pdf
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In vitro study on sperm competition in common carp (Cyprinus carpio L.) ArchiMer
Kaspar, Vojtech; Hulak, Martin; Kohlmann, Klaus; Vandeputte, Marc; Rodina, Marek; Gela, David; Linhart, Otomar.
Sperm competition occurs when sperm from different males compete for fertilization. The aim of this in vitro fertilization study was to compare the competitive success of five males using heterosperm with an equal number of sperm from each male and four different sperm/egg ratios (5,000, 10,000, 20,000 and 100,000 spermatozoa per egg) in order to better understand variations of competitive fertilization. The roles of sperm motility and velocity were studied. Fertilization and hatching rates of the 5 males studied, with 100,000 spermatozoa per egg, were between 23.7-94.8% and 23.7-92.2%, respectively. Sperm velocity and percentage of motile sperm ranged from 85.0 to 137.6 mu m s(-1) and 2.0 to 93.5% at 15 see post sperm activation, respectively. The...
Tipo: Text Palavras-chave: Paternity assignment; Artificial reproduction; Aquaculture.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6551.pdf
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Can individual feed conversion ratio at commercial size be predicted from juvenile performance in individually reared Nile tilapia Oreochromis niloticus? ArchiMer
Rodde, Charles; Chatain, Beatrice; Vandeputte, Marc; Quoc Trinh, Trong; Benzie, John A.h.; De Verdal, Hugues.
Feed conversion ratio (FCR), the ratio between feed intake and body weight gain, is of major interest for improving aquaculture sustainability through reduced feed costs and environmental impacts. Demonstrating whether FCR measured in juvenile fish is an accurate predictor of their performance during the whole rearing period is critical to developing genetic improvement programs for this trait. This is especially true for estimates obtained in individually reared fish, for which this has high implications regarding the size of the necessary rearing structures. We obtained individual FCR from 30 male Nile tilapia Oreochromis niloticus from the GIFT strain individually reared in a recirculating system, from 36 to 260 g mean weight. They were fed twice a day...
Tipo: Text Palavras-chave: Feed efficiency; GIFT tilapia; Individual rearing; Selective breeding.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00630/74175/73757.pdf
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The Effects of Feed Restriction and Isolated or Group Rearing on the Measurement of Individual Feed Intake and Estimation of Feed Conversion Ratio in Juvenile Nile Tilapia (Oreochromis niloticus) for Selective Breeding Purposes ArchiMer
Rodde, Charles; Vandeputte, Marc; Quoc Trinh, Trong; Douchet, Vincent; Canonne, Marc; Benzie, John A. H.; De Verdal, Hugues.
Accurately measuring the phenotype at the individual level is critical to the success of selective breeding programs. Feed efficiency is a key sustainability trait and is typically approached through feed conversion ratio (FCR). This requires measurements of body weight gain (BWG) and feed intake (FI), the latter being technically challenging in fish. We assessed two of the principal methods for measuring feed intake in fish over consecutive days: (1) group rearing 10 fish per group and video recording the meals and (2) rearing fish individually on a restricted ration. Juvenile Nile tilapia (Oreochromis niloticus) from the Genetically Improved Farmed Tilapia (GIFT) strain and the Cirad strain were entered into the study (128 GIFT and 109 Cirad). The GIFT...
Tipo: Text Palavras-chave: Feed efficiency; Individual rearing; Group rearing; Fish; Video analyses; Feed intake.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00677/78924/81292.pdf
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Measuring individual feed efficiency and its correlations with performance traits in Nile tilapia, Oreochromis niloticus ArchiMer
De Verdal, Hugues; Mekkawy, Wagdy; Lind, Curtis E.; Vandeputte, Marc; Chatain, Beatrice; Benzie, John A. H..
Estimating individual feed intake of fish held in groups has long been a challenge precluding precise knowledge of the individual variation of feed efficiency (FE) in fish. In this study, counts of the number of feed pellets (1.63 mg on average) eaten by individual tilapia (Oreochromis niloticus) held in 8 mixed sex groups of 15 fish were measured from video recordings made over a period of 10 days where fish were fed twice daily to achieve compensatory growth after 10 days of fasting. The initial body weight of the fish was 9.77 ± 2.03 g. Accumulated measures of feed intake (FI) over 11 meals were found to achieve 95% repeatability and a high accuracy of estimation of FI. During the FI measurement period, the average fish growth was 12.0 ± 3.6 g, feed...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46832/46707.pdf
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Effect of production quotas on economic and environmental values of growth rate and feed efficiency in sea cage fish farming ArchiMer
Besson, Mathieu; De Boer, I. J. M.; Vandeputte, Marc; Van Arendonk, J. A. M.; Quillet, E.; Komen, H.; Aubin, J..
In sea cage fish farming, production quotas aim to constrain the impact of fish farming on the surrounding ecosystem. It is unknown how these quotas affect economic profitability and environmental impact of genetic improvement. We combined bioeconomic modelling with life cycle assessment (LCA) to calculate the economic (EV) and environmental (ENV) values of thermal growth coefficient (TGC) and feed conversion ratio (FCR) of sea bass reared in sea cages, given four types of quota commonly used in Europe: annual production (Qprod), annual feed distributed (Qannual_ feed), standing stock (Qstock), and daily feed distributed (Qdaily_ feed). ENV were calculated for LCA impact categories climate change, eutrophication and acidification. ENV were expressed per...
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Ano: 2017 URL: http://archimer.ifremer.fr/doc/00377/48778/49176.pdf
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Genotype by diet interactions in European sea bass (Dicentrarchus labrax L.): Nutritional challenge with totally plant-based diets ArchiMer
Le Boucher, Richard; Vandeputte, Marc; Dupont-nivet, M.; Quillet, E.; Ruelle, Francois; Vergnet, Alain; Kaushik, Sadasivam; Allamellou, J. M.; Medale, F.; Chatain, Beatrice.
Aquaculture of carnivorous species has strongly relied on fish meal and fish oil for feed formulation; however, greater replacement by terrestrial plant-based products is occurring now. This rapid change in dietary environment has been a major revolution and has to be taken into consideration in breeding programs. The present study analyzes potential consequences of this nutritional tendency for selective breeding by estimating genetic parameters of BW and growth rates estimated by the thermal growth coefficient (TGC) over different periods with extremely different diets. European sea bass (Dicentrarchus labrax L.) from a factorial cross (1,526 fish) between 25 sires and 9 dams were used to estimate heritabilities and genotype by diet interaction. Starting...
Tipo: Text Palavras-chave: European sea bass; Genetic correlation; Genotype by diet interaction; Genotype by environment interaction; Plant-based diet.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00146/25711/26299.pdf
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Estimates of genetic variability and inbreeding in experimentally selected populations of European sea bass ArchiMer
Hillen, J. E. J.; Coscia, I.; Vandeputte, Marc; Herten, K.; Hellemans, B.; Maroso, F.; Vergnet, Alain; Allal, Francois; Maes, G. E.; Volckaert, F. A. M..
The aquaculture industry has increasingly aimed at improving economically important traits like growth, feed efficiency and resistance to infections. Artificial selection represents an important window of opportunity to significantly improve production. However, the pitfall is that selection will reduce genetic diversity and increase inbreeding in the farmed stocks. Genetic tools are very useful in this context as they provide accurate measures of genetic diversity together with many additional insights in the stock status and the selection process. In this study we assessed the level of genetic variability and relatedness over several generations of two lines of experimentally selected European sea bass (Dicentrarchus labrax L.). The first line was...
Tipo: Text Palavras-chave: Artificial selection; DdRAD; Fish; Genetic diversity; Genomics; Inbreeding.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00392/50314/50993.pdf
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Gene-centromere mapping in meiotic gynogenetic European seabass ArchiMer
Oral, Munevver; Colleter, Julie; Bekaert, Michhttps://w3.ifremer.fr/archimer-admin/author.jsp#ael; Taggart, John B.; Palaiokostas, Christos; Mcandrew, Brendan J.; Vandeputte, Marc; Chatain, Beatrice; Kuhl, Heiner; Reinhardt, Richard; Peruzzi, Stefano; Penman, David J..
Background Fully isogenic lines in fish can be developed using “mitotic” gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families. Results From a single meiotic gynogenetic family consisting of 79 progeny, 42...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Meiotic gynogenesis; Isogenic lines; DdRAD seq; Genetic map; Gene-Centromere map; Aquaculture.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49901/50462.pdf
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Combining vitassign and colony: an efficient practical procedure for parental assignment with missing parents ArchiMer
Doan, Q.k.; Vandeputte, Marc; Chatain, Beatrice; Vergnet, Alain; Allal, Francois.
Tipo: Text
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00281/39196/37750.pdf
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Heritability of Boldness and Hypoxia Avoidance in European Seabass, Dicentrarchus labrax ArchiMer
Ferrari, Sebastien; Horri, Khaled; Allal, Francois; Vergnet, Alain; Benhaim, David; Vandeputte, Marc; Chatain, Beatrice; Begout, Marie-laure.
To understand the genetic basis of coping style in European seabass, fish from a full factorial mating (10 females x 50 males) were reared in common garden and individually tagged. Individuals coping style was characterized through behavior tests at four different ages, categorizing fish into proactive or reactive: a hypoxia avoidance test (at 255 days post hatching, dph) and 3 risk-taking tests (at 276, 286 and 304 dph). We observed significant heritability of the coping style, higher for the average of risk-taking scores (h2 = 0.45 ± 0.14) than for the hypoxia avoidance test (h2 = 0.19 ± 0.10). The genetic correlations between the three risk-taking scores were very high (rA = 0.96–0.99) showing that although their repeatability was moderately high (rP =...
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Ano: 2016 URL: https://archimer.ifremer.fr/doc/00362/47278/47239.pdf
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Negative genetic correlations between production traits and head or bony tissues in large all-female rainbow trout (Oncorhynchus mykiss) ArchiMer
Haffray, Pierrick; Bugeon, Jerome; Pincent, Cedric; Chapuis, Herve; Mazeiraud, Emmanuel; Rossignol, Marie-noelle; Chatain, Beatrice; Vandeputte, Marc; Dupont-nivet, Mathilde.
Genetic parameters of production traits (growth, carcass yield, fillet yield) and bony tissues (head and vertebral axis) were estimated for large all-female rainbow trout Oncorhynchus mykiss reared in freshwater. Genetic parameters were estimated using REML at 16 months of age (1636 g) on 1962 DNA-assigned progenies from a partial factorial mating design with 60 dams and 100 sex-reversed sires. Most traits presented medium to high heritability (0.37 to 0.54). A high genetic correlation (r(A)=0.97) was found between fillet yield and headless gutted carcass yield (or HGCarc%). Due to its higher heritability and high genetic correlation with fillet yield, selection on HGCarc% should result in a 50% increase in selection efficiency on fillet yield by reducing...
Tipo: Text Palavras-chave: Resource allocation; Fillet yield; Head; Salmonids; Aquaculture; Selective breeding.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00109/22042/19801.pdf
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An accurate formula to calculate exclusion power of marker sets in parentage assignment ArchiMer
Vandeputte, Marc.
In studies on parentage assignment with both parents unknown, the exclusion power of a marker set is generally computed under the hypothesis that the potential families tested are independent and unrelated samples. This tends to produce overly optimistic exclusion power estimates. In this work, we have developed a new formula that gives almost unbiased results at the population level.
Tipo: Text
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00117/22804/20610.pdf
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