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| Registros recuperados: 29 | |
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| Vezilier, Julien; Nicot, Antoine; De Lorgeril, Julien; Gandon, Sylvain; Rivero, Ana. |
| Because of their role as vectors of diseases, the evolution of insecticide resistance in mosquitoes has been intensively investigated. Insecticide resistance is associated to a wide range of pleiotropic effects on several key life-history traits of mosquitoes such as longevity and behavior. However, despite its potential implications in pathogen transmission, the effects of insecticide resistance on mosquito immunity have received little, if any, attention. Here, we investigate the impact of insecticide resistance in Culex pipiens, an epidemiologically important vector of a wide array of pathogens. Using both isogenic laboratory strains and field-caught mosquitoes, we investigate the impact of two main insecticide resistance mechanisms (metabolic... |
| Tipo: Text |
Palavras-chave: Acetylcholinesterase; Anopheles; Antimicrobial peptide; Carboxylesterases; Nitric oxide; Transferrin. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00136/24773/22845.pdf |
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| Durieux, Eric; Farver, Thomas B.; Fitzgerald, Patrick S.; Eder, Kai J.; Ostrach, David J.. |
| Acetylcholinesterase (AChE) activity is one of the most common biomarkers of neurotoxicity used in aquatic organisms. However, compared to its extensive use as biomarker, the effects of natural factors onAChE activity remain unclear especially in estuarine fishes. The aim of this study was to evaluate the effects of natural factors on AChE activity of striped bass (Morone saxatilis) juveniles. Brain AChE activity was measured in YOY (Young-Of-Year) individuals collected monthly from August 2007 to January 2008 at 12 different sites in the San Francisco Estuary system. The spatio-temporal variability of AChE was analyzed relative to water temperature and salinity as well as fish size. AChE activity was highly positively correlated with water temperature and... |
| Tipo: Text |
Palavras-chave: Fish; Biomarker; Acetylcholinesterase; Brain; Temperature; Salinity; Size. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00008/11939/8642.pdf |
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| Janardhan,Bhavya; Shrikanth,Vineetha M; Mirajkar,Kiran K; More,Sunil S. |
| Background : Snakebites are considered a neglected tropical disease that affects thousands of people worldwide. Although antivenom is the only treatment available, it is associated with several side effects. As an alternative, plants have been extensively studied in order to obtain an alternative treatment. In folk medicine, Azima tetracanthaLam. is usually used to treat snakebites. The present study aims to provide a scientific explanation for the use of this plant against snakebite. The extracts of shade dried leaves of A. tetracanthawere tested for in vitroinhibitory activity on toxic venom enzymes like phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase etc. from Bungarus caeruleusandVipera russellivenoms.Results : The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Azima tetracantha; Antivenom; Krait; Viper; In vitro. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200318 |
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| Ozkan,O.; Adiguzel,S.; Kar,S.; Kurt,M.; Yakistiran,S.; Cesaretli,Y.; Orman,M.; Karaer,Z.. |
| Scorpions can be considered living fossils because they have changed so little during the last 400 million years. They are venomous arthropods of the Arachnida class and regarded as relatives of spiders, ticks and mites. The aim of the present study was to evaluate the toxicity of Androctonus crassicauda (Olivier, 1807) venom and its effects on the acetylcholinesterase (AchE) activity and on electrolytes levels in rats. Animals were divided into seven groups of five rats each. Test groups received 250µg/kg of venom solution while control group was treated with 200µl of physiological saline solution (PSS). Blood samples were collected from the animals on the 1st, 2nd 4th, 8th, 12th, and 24th hours after subcutaneous injection of venom. Animals were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpion; Androctonus crassicauda; Venom; Acetylcholinesterase; Electrolytes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100005 |
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| Ahmed,M; Latif,N; Khan,RA; Ahmad,A; Rocha,JBT; Mazzanti,CM; Bagatini,MD; Morsch,VM; Schetinger,MRC. |
| This study analyses venom from the elapid krait snake Bungarus sindanus, which contains a high level of acetylcholinesterase (AChE) activity. The enzyme showed optimum activity at alkaline pH (8.5) and 45ºC. Krait venom AChE was inhibited by substrate. Inhibition was significantly reduced by using a high ionic strength buffer; low ionic strength buffer (10 mM PO4 pH 7.5) inhibited the enzyme by 1. 5mM AcSCh, while high ionic strength buffer (62 mM PO4 pH 7.5) inhibited it by 1 mM AcSCh. Venom acetylcholinesterase was also found to be thermally stable at 45ºC; it only lost 5% of its activity after incubation at 45ºC for 40 minutes. The Michaelis-Menten constant (Km) for acetylthiocholine iodide hydrolysis was found to be 0.068 mM. Krait venom... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Inhibition; Krait; Ionic strength; Acetylthiocholine iodide; Bungarus sindanus; Snake venom. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200014 |
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| Rebechi,Débora; Richardi,Vinicius S.; Vicentini,Maiara; Guiloski,Izonete C.; Assis,Helena C. Silva de; Navarro-Silva,Mário A.. |
| Low malathion concentrations influence metabolism in Chironomus sancticaroli (Diptera, Chironomidae) in acute and chronic toxicity tests. Organophosphate compounds are used in agro-systems, and in programs to control pathogen vectors. Because they are continuously applied, organophosphates often reach water sources and may have an impact on aquatic life. The effects of acute and chronic exposure to the organophosphate insecticide malathion on the midge Chironomus sancticaroli are evaluated. To that end, three biochemical biomarkers, acetylcholinesterase (AChE), alpha (EST-α) and beta (EST-β) esterase were used. Acute bioassays with five concentrations of malathion, and chronic bioassays with two concentrations of malathion were carried out. In the acute... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Bioindicator; Esterases; Organophosphate; Toxicity. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262014000300012 |
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| CHAVES,Valesca de Souza; MARCON,Jaydione Luiz; DUNCAN,Wallice Paxiúba; ALVES-GOMES,José Antônio. |
| ABSTRACT Deltamethrin is a pyrethroid insecticide widely used in pest control in Brazilian agriculture. The intensive and disordered use of this pesticide in the Amazon region can drive it into aquatic ecosystems in several ways, but mainly by runoff and leaching. The present study was conceived to determine the acute toxicity (LC50) of a deltamethrin based pesticide (DBP) and to characterize its effects on two biochemical biomarkers, glutathione-S-transferase (GST) and acetylcholinesterase (AChE), in tissues of the electric fish Microsternarchus cf. bilineatus. Fishes were exposed to concentrations of 1, 2, 3, 4 and 5 μg L-1 of DBP for up to 96 hours. For each treatment, the absolute activity of the enzymes GST (muscle and liver) and AChE (muscle and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pyrethroid insecticide; Biomarkers; Median LC50; Glutathione-S-transferase; Acetylcholinesterase. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672020000400355 |
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| Morais, Daniel Vieira de; Moreira, Manuela Maria; Silva, Fabiane de Lima; Costa, Maria Angélica Pereira de Carvalho; Delerue-Mato, Cristina; Carvalho, Carlos Alfredo Lopes de; Estevinho, Maria Leticia Miranda. |
| For the first time, the anti-hemolytic activity and the enzyme inhibitory activities of Dalbergia ecastaphyllum leaves extracts were tested against α-amylase, α-glucosidase, lipase, acetylcholinesterase, butyrylcholinesterase, tyrosinase and hyaluronidase. The phenolic profile of the obtained extracts was also investigated by high-performance liquid chromatography with photodiode array detection (HPLC-PAD). The extracts showed inhibitory activity against all enzymes evaluated, with the highest inhibitory activity reported for the enzyme hyaluronidase (28.28 ± 2.43 to 72.19 ± 1.40 μg mL-1). The obtained extracts also demonstrate anti-hemolytic activity (52.22 ± 1.62 to 71.17 ± 1.82%). Among the phenolic compounds identified, protocatechuic, vanillic and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Α-glucosidase; Hyaluronidase; Preclinical research.. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46622 |
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| GUIMARÃES,CARINE A.; BIELLA,MAIRIS S.; LOPES,ABIGAIL; DEROZA,PEDRO F.; OLIVEIRA,MARIANA B.; MACAN,TAMIRES P.; STRECK,EMILIO L.; FERREIRA,GUSTAVO C.; ZUGNO,ALEXANDRA I.; SCHUCK,PATRÍCIA F.. |
| Increased fructose concentrations are the biochemical hallmark of fructosemia, a group of inherited disorders on the metabolic pathway of this sugar. The main clinical findings observed in patients affected by fructosemia include neurological abnormalities with developmental delay, whose pathophysiology is still undefined. In the present work we investigated the in vitro and in vivo effects of fructose on acetylcholinesterase (AchE) activity in brain structures of developing rats. For the in vitro experiments, fructose was added at increasing concentrations to the incubation medium. It was observed that fructose provoked an inhibition of acetylcholinesterase activity in cerebral cortex of 30-day-old-rats, even at low concentrations (0.1 mM). For the in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Brain; Fructosel hereditary fructose intolerance. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000401919 |
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| MÜLLER,LUCIELI K.F.; SILVA,ALEKSANDRO S. DA; BOTTARI,NATHIELI B.; SANTURIO,JANIO M.; MORSCH,VERA M.; PIVA,MANOELA M.; MENDES,RICARDO E.; GLORIA,EDUARDO M.; PAIANO,DIOVANI. |
| Abstract: The aim of this study was to evaluate the effect of spray-dried porcine plasma (SDPP) supplementation on cholinesterase enzymes and its relationship with animal behavior of weaning piglets exposed to mycotoxin contaminated diets. To achieve these objectives, two experimental design approaches were used. Male piglets (7.15±0.61kg) were allocated in four groups: CTL group received a regular diet; SDPP group received a regular diet and 6% SDPP; MYC group received a diet containing desired contamination of 210 µg/kg aflatoxins and 6.690 µg/kg fumonisins; group MYC+SDPP received 253 µg/kg aflatoxins, 6930 µg/kg fumonisins and 6% SDPP. The animals treated with mycotoxin co-contaminated diets showed an increase in AChE and BChE activities in peripheral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycotoxin; Acetylcholinesterase; Brain; Spray-dried porcine plasma; Animal health. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300807 |
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| TEODORAK,BRENA P.; FERREIRA,GABRIELA K.; SCAINI,GISELLI; WESSLER,LETÍCIA B.; HEYLMANN,ALEXANDRA S.; DEROZA,PEDRO; VALVASSORI,SAMIRA S.; ZUGNO,ALEXANDRA I.; QUEVEDO,JOÃO; STRECK,EMILIO L.. |
| Fenproporex is the second most commonly amphetamine-based anorectic consumed worldwide; this drug is rapidly converted into amphetamine, in vivo, and acts by increasing dopamine levels in the synaptic cleft. Considering that fenproporex effects on the central nervous system are still poorly known and that acetylcholinesterase is a regulatory enzyme which is involved in cholinergic synapses and may indirectly modulate the release of dopamine, the present study investigated the effects of acute administration of fenproporex on acetylcholinesterase activity in brain of young rats. Young male Wistar rats received a single injection of fenproporex (6.25, 12.5 or 25mg/kg i.p.) or vehicle (2% Tween 80). Two hours after the injection, the rats were killed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acetylcholinesterase; Amphetamine; Dopamine; Fenproporex. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000301389 |
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| DAMAZIO,LOUYSE S.; SILVEIRA,FLÁVIA R.; CANEVER,LARA; CASTRO,ADALBERTO A. DE; ESTRELA,JADNE M.; BUDNI,JOSIANE; ZUGNO,ALEXANDRA I.. |
| ABSTRACT Studies have shown that schizophrenic patients seem to have nutritional deficiencies. Ascorbic acid (AA) has an important antioxidant effect and neuromodulatory properties. The aim of this study was to evaluate the effects of AA on locomotor activity and the acetylcholinesterase activity (AChE) in an animal model of schizophrenia (SZ). Rats were supplemented with AA (0.1, 1, or 10 mg/kg), or water for 14 days (gavage). Between the 9th and 15th days, the animals received Ketamine (Ket) (25 mg/kg) or saline (i.p). After the last administration (30 min) rats were subjected to the behavioral test. Brain structures were dissected for biochemical analysis. There was a significant increase in the locomotor activity in Ket treated. AA prevented the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Schizophrenia; Ascorbic acid; Ketamine; Hyperlocomotion; Acetylcholinesterase. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301133 |
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| Ahmad,Sajjad; Ullah,Farhat; Ayaz,Muhammad; Sadiq,Abdul; Imran,Muhammad. |
| BACKGROUND: Rumex species are traditionally used for the treatment of neurological disorders including headache, migraine, depression, paralysis etc. Several species have been scientifically validated for antioxidant and anticholinestrase potentials. This study aims to investigate Rumex hastatus D. Don crude methanolic extract, subsequent fractions, saponins and flavonoids for acetylcholinestrase, butyrylcholinestrase inhibition and diverse antioxidant activities to validate its folkloric uses in neurological disorders. Rumexhastatus crude methanolic extract (Rh. Cr), subsequent fractions; n-hexane (Rh. Hex), chloroform (Rh. Chf), ethyl acetate (Rh. EtAc), aqueous fraction (Rh. Aq), crude saponins (Rh. Sp) and flavonoids (Rh. Fl) were investigated against... |
| Tipo: Journal article |
Palavras-chave: Ethnomedicinal; Acetylcholinesterase; Butyrycholinesterase; DPPH; ABTS. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100020 |
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| Costa Marques,Thiago H; Santos De Melo,Cassio H; Fonseca De Carvalho,Rusbene B; Costa,Luciana M; De Souza,Alexandre A; David,Jorge M; De Lima David,Juceni P; De Freitas,Rivelilson M. |
| This study describes the isolation and identification of apigenin-7-O-ghicopyranoside, a flavonoid isolated from the flowers of Bellis perennis L., Asteraceae, an species with a broad spectrum of biological activities. The in vitro antioxidant activity and the inhibition of the enzyme acetylcholinesterase were evaluated. The flavonoid showed strong in vitro antioxidant potential, because of the capacity of removal of hydroxyl radicals and nitric oxide, and also prevented the formation of thiobarbituric acid-reactive substances. These parameters were inhibited at the highest concentration of ApG at rates of 77.7%, 72% and 73.4%, respectively, in addition to inhibiting acetylcholinesterase, suggesting potential use in the treatment of neurodegenerative... |
| Tipo: Journal article |
Palavras-chave: Acetylcholinesterase; Antioxidant; Bellis perennis; Flavonoid. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000300002 |
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| Dzoyem,Jean P.; Tsamo,Armelle T.; Melong,Raduis; Mkounga,Pierre; Nkengfack,Augustin E.; McGaw,Lyndy J.; Eloff,Jacobus N.. |
| BACKGROUND: Limonoids are highly oxygenated compounds with a prototypical structure. Their occurrence in the plant kingdom is mainly confined to plant families of Meliaceae and Rutaceae. Owing to their wide range of pharmacological and therapeutic properties, this study was aimed at investigating the potential nitric oxide (NO) and acetylcholinesterase (AChE) inhibitory activity and the cytotoxicity of three limonoids: trichilia lactone D5 (1), rohituka 3 (2) and dregeanin DM4 (3), isolated from Trichilia welwitschii C.DC RESULTS: Results indicated that the three limonoids had low cytotoxicity towards Vero cells with LC50 values ranging from 89.17 to 75.82 (μg/L. Compounds (2) and (3) had lower cytotoxicity compared to puromycin and doxorubicin... |
| Tipo: Journal article |
Palavras-chave: Trichilia welwitschii; Cytotoxicity; Acetylcholinesterase; Nitric oxide; Limonoids. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100057 |
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| Registros recuperados: 29 | |
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