|
|
|
| Registros recuperados: 67 | |
|
| |
|
| |
|
|
| Brincas,Heloisa Magagnin; Augusto,Danillo G.; Mathias,Carolina; Cavalli,Iglenir João; Lima,Rubens Silveira de; Kuroda,Flávia; Urban,Cícero de Andrade; Gradia,Daniela Fiori; de Oliveira,Jaqueline; de Almeida,Rodrigo Coutinho; Ribeiro,Enilze Maria de Souza Fonseca. |
| Abstract MicroRNAs (miRNAs) play an essential role in gene expression and affect the development of tumours, including breast cancer (BC). Polymorphisms in miRNA genes can affect the interaction of miRNAs with their target messenger RNA by interfering, creating or disrupting target sites. The single nucleotide polymorphism (SNP) rs2910164, located in the seed region of miR146a, was shown to be associated with BC among different populations. In the present study, we investigated whether rs2910164 is associated with BC in 326 patients and 411 controls from a Brazilian population of predominantly European ancestry. The presence of the allele rs2910164*C was associated with an increased risk of BC (OR=1.4, 95% CI=1.03-1.85, p = 0.03). We also analysed publicly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MiRNA polymorphism; MiR146a; Rs2910164; Breast cancer; Case-control study. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500103 |
| |
|
|
| Santos,Raquel A.; Teixeira,Ana Claudia; Mayorano,Monica B.; Carrara,Helio H.A.; Andrade,Jurandyr M.; Takahashi,Catarina S.. |
| Breast cancer (BC) is the most prevalent type worldwide, besides being one of the most common causes of death among women. It has been suggested that sporadic BC is most likely caused by low-penetrance genes, including those involved in DNA repair mechanisms. Furthermore, the accumulation of DNA damage may contribute to breast carcinogenesis. In the present study, the relationship between two DNA repair genes, viz., XRCC1 (Arg399Gln) and XRCC3 (Thr241Met) polymorphisms, and the levels of chromosome damage detected in 65 untreated BC women and 85 healthy controls, was investigated. Chromosome damage was evaluated through micronucleus assaying, and genotypes determined by PCR-RFLP methodology. The results showed no alteration in the risk of BC and DNA damage... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA repair polymorphisms; Breast cancer; Micronucleus assay. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400007 |
| |
|
|
| Palmero,Edenir I.; Caleffi,Maira; Schüler-Faccini,Lavínia; Roth,Fernanda L.; Kalakun,Luciane; Netto,Cristina Brinkmann Oliveira; Skonieski,Giovana; Giacomazzi,Juliana; Weber,Bernadete; Giugliani,Roberto; Camey,Suzi A.; Ashton-Prolla,Patricia. |
| In 2004, a population-based cohort (the Núcleo Mama Porto Alegre - NMPOA Cohort) was started in Porto Alegre, southern Brazil and within that cohort, a hereditary breast cancer study was initiated, aiming to determine the prevalence of hereditary breast cancer phenotypes and evaluate acceptance of a genetic cancer risk assessment (GCRA) program. Women from that cohort who reported a positive family history of cancer were referred to GCRA. Of the 9218 women enrolled, 1286 (13.9%) reported a family history of cancer. Of the 902 women who attended GCRA, 55 (8%) had an estimated lifetime risk of breast cancer ³ 20% and 214 (23.7%) had pedigrees suggestive of a breast cancer predisposition syndrome; an unexpectedly high number of these fulfilled criteria for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; Genetic counseling; Hereditary cancer syndromes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300004 |
| |
|
|
| Andrade,Kelvin C.; Santiago,Karina M.; Fortes,Fernanda P.; Mambelli,Lisley I.; Nóbrega,Amanda F.; Achatz,Maria I.. |
| Abstract Germline TP53 mutations are associated with Li-Fraumeni syndrome (LFS), a disease that predisposes carriers to a wide variety of early onset tumors. In southern and southeastern Brazil, a high frequency of a germline TP53 mutation, p.R337H, was diagnosed in 0,3% of the population due to a founder effect. Carriers are at risk for developing cancer but the penetrance is lower than in typical DNA binding domain mutations. To date, only a few families were detected and diagnosis of carriers remains a challenge. Therefore, the inclusion of additional criteria to detect p.R337H carriers is necessary for the Brazilian population. We assessed the A.C. Camargo Cancer Center Oncogenetics Department database in search of common characteristics associated... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Breast cancer; Li-Fraumeni syndrome; P.R337H; TP53. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200199 |
| |
|
|
| Wu,Xiang-mei; Liu,Xing; Bu,You-quan; Sengupta,Joyeeta; Cui,Hong-juan; Yi,Fa-ping; Liu,Tao; Yuan,Chen-fu; Shi,Yan-yan; Song,Fang-zhou. |
| The oncogene Bmi-1 is a member of the Polycomb group gene family. Its expression is found to be greatly increased in a number of malignant tumors including breast cancer. This could suggest Bmi-1 as a potent therapeutic target. In this study, RNAi was introduced to down-regulate the expression of Bmi-1 in a highly malignant breast adenocarcinoma cell line, MCF-7. A thorough study of the biological behavior and chemosensitivity changes of the MCF-7 cells was carried out in context to the therapeutic potential of Bmi-1. The results obtained indicated that siRNA targeting of Bmi-1 could lead to an efficient and specific inhibition of endogenous Bmi-1 activity. The mRNA and protein expression of Bmi-1 were determined by RT-PCR and Western blot, respectively.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNA interference; Bmi-1; Retrovirus vector; Doxorubicin; Breast cancer. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400004 |
| |
|
|
| Campanella,Nathália Cristina; Demartini,Mariana da Silva; Torres,Claudia; Almeida,Eduardo Tonon de; Gouvêa,Cibele Marli Cação Paiva. |
| The antitumorigenic potential of two palladium(II) complexes, [Pd(ca2-o-phen)Cl2 ] - C1 and [Pd(dmba)(dppp)Cl] - C2, was evaluated, using MDA-MB-435 cells, a human breast adenocarcinoma cell-line that does not express the estrogen receptor α (ER-). Growth inhibition and induced alterations in cell-morphology were analyzed. The sulforhodamine B test showed that, compared to control cells, both C1 and C2 significantly inhibited (p < 0.5) cell growth. The maximum effect with both was achieved with 1 µM complexes, after 24 h of treatment. No further cell-growth inhibition was achieved by increasing concentration or incubation time. Cell morphology was analyzed after staining with hematoxylin-eosin (HE). The morphological changes noted in the treated cells... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adenocarcinoma cells; Antitumor; Breast cancer; Chemotherapy; Coordination complexes. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100023 |
| |
|
|
| Fazza,Ana Cristina; Sabino,Flavia Cal; Setta,Nathalia de; Bordin Jr.,Newton Antonio; Silva,Eloiza Helena Tajara da; Carareto,Claudia Marcia Aparecida. |
| Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alu-PCR; Breast cancer; Gene insertions; Gene deletions; Invasive ductal carcinoma; Recombination. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100004 |
| |
|
|
| Lourenço,Juliano Javert; Vargas,Fernando R.; Bines,José; Santos,Elizete M.; Lasmar,Cezar A. P.; Costa,Célia H.; Teixeira,Eliane M. B.; Maia,Maria C. M.; Coura,Fátima; Silva,Carlos H. D.; Moreira,Miguel A. M.. |
| BRCA1 mutations are known to be responsible for the majority of hereditary breast and ovarian cancers in women with early onset and a family history of the disease. In this paper we present a mutational survey conducted in 47 Brazilian patients with breast/ovarian cancer, selected based on age at diagnosis, family history, tumor laterality, and presence of breast cancer in male patients. All 22 coding exons and intron-exon junctions were sequenced. Constitutional mutations were found in seven families, consisting of one insertion (insC5382) in exon 20 (four patients), one four base-pair deletion (3450-3453delCAAG) in exon 11 resulting in a premature stop codon (one patient), one transition (IVS17+2T> C) in intron 17 affecting a mRNA splicing site (one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BRCA1; Breast cancer; Ovarian cancer. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400006 |
| |
|
|
| Dillenburg,Crisle Vignol; Bandeira,Isabel Cristina; Tubino,Taiana Valente; Rossato,Luciana Grazziotin; Dias,Eleonora Souza; Bittelbrunn,Ana Cristina; Leistner-Segal,Sandra. |
| Certain mutations in BRCA1 and BRCA2 genes are frequent in the Ashkenazi Jewish population. Several factors contribute to this increased frequency, including consanguineous marriages and an event known as a "bottleneck', which occurred in the past and caused a drastic reduction in the genetic variability of this population. Several studies were performed over the years in an attempt to elucidate the role of BRCA1 and BRCA2 genes in susceptibility to breast cancer. The aim of this study was to estimate the carrier frequency of certain common mutations in the BRCA1 (185delAG and 5382insC) and BRCA2 (6174delT) genes in an Ashkenazi Jewish population from Porto Alegre, Brazil. Molecular analyses were done by PCR followed by RFLP (ACRS). The carrier frequencies... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ashkenazi Jews; Breast cancer; BRCA1; BRCA2; Mutation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400009 |
| |
|
|
| Ewald,Ingrid Petroni; Cossio,Silvia Liliana; Palmero,Edenir Inez; Pinheiro,Manuela; Nascimento,Ivana Lucia de Oliveira; Machado,Taisa Manuela Bonfim; Sandes,Kiyoko Abe; Toralles,Betânia; Garicochea,Bernardo; Izetti,Patricia; Pereira,Maria Luiza Saraiva; Bock,Hugo; Vargas,Fernando Regla; Moreira,Miguel Ângelo Martins; Peixoto,Ana; Teixeira,Manuel R.; Ashton-Prolla,Patricia. |
| Abstract Approximately 5-10% of breast cancers are caused by germline mutations in high penetrance predisposition genes. Among these, BRCA1 and BRCA2, which are associated with the Hereditary Breast and Ovarian Cancer (HBOC) syndrome, are the most frequently affected genes. Recent studies confirm that gene rearrangements, especially in BRCA1, are responsible for a significant proportion of mutations in certain populations. In this study we determined the prevalence of BRCA rearrangements in 145 unrelated Brazilian individuals at risk for HBOC syndrome who had not been previously tested for BRCA mutations. Using Multiplex Ligation-dependent Probe Amplification (MLPA) and a specific PCR-based protocol to identify a Portuguese founder BRCA2 mutation, we... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; Hereditary Breast and Ovarian Cancer syndrome; Gene rearrangements; BRCA gene. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200223 |
| |
|
|
| Güllü,Gökçe; Peker,Irem; Haholu,Aptullah; Eren,Fatih; Küçükodaci,Zafer; Güleç,Bülent; Baloglu,Hüseyin; Erzik,Can; Özer,Ayse; Akkiprik,Mustafa. |
| The functional role of IGFBP5 in breast cancer is complicated. Experimental and bioinformatics studies have shown that IGFBP5 is targeted by miR-140-5p and miR-193b, although this has not yet been proven in clinical samples. The aim of this study was to evaluate the expression of miR-140-5p and miR-193b in breast cancer and adjacent normal tissue and assess its correlation with IGFBP5 and the clinicopathological characteristics of the tumors. IGFBP5 protein expression was analyzed immunohistochemically and IGFBP5, miR-140 and miR-193b mRNA expression levels were analyzed with real-time RT-PCR. Tumor tissue had higher miR-140-5p expression than adjacent normal tissue (p = 0.015). Samples with no immunohistochemical staining for IGFBP5 showed increased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; ER alpha; IGFBP5; Micro RNA; MiR-140; MiR-193b. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100021 |
| |
|
| |
|
| |
|
|
| Saglican,Yesim; Ince,Ümit. |
| HER2 amplification or overexpression is considered as disease outcome and a predictive marker of response to treatment in breast cancer. The present study aimed to compare the results of IHC and FISH for determining HER2 and to search the interpretational differences. Samples (n= 169), of which 31 were the paraffin blocks sent from outer centers, that underwent FISH analysis for HER-2 were included. Samples were re-reviewed by IHC in our laboratory. FISH test was negative in 131 (77.5%) and positive in 38 (22.5%). When those with previous IHC 0-1+ were re-reviewed, the results were found again 0-1+ and none of them was FISH positive. Inconsistency between re-reviewed IHC and previous IHC results was 25% for those with 2+ score and 11% for those with 3+... |
| Tipo: Journal article |
Palavras-chave: Breast cancer; HER2; Immunocytochemistry; FISH technique. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000200051 |
| |
|
| |
|
|
| Bezerra,Patrícia Heloise Alves; Ferreira,Isadora Marques; Franceschi,Beatriz Tinoco; Bianchini,Francine; Ambrósio,Luciana; Cintra,Adélia Cristina O.; Sampaio,Suely Vilela; Castro,Fabíola Attié de; Torqueti,Maria Regina. |
| ABSTRACT Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines. Methods: BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solution method, annexin-V-FITC/propidium iodide staining and by apoptotic/autophagic protein expression. Cancer stem cells (CSCs) were quantified by flow cytometry using anti-CD24-FITC and anti-CD44-APC antibodies and propidium iodide. Results: BthTX-I at 102 µg/mL induced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Bothropstoxin; Breast cancer; Cancer stem cells. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100315 |
| |
|
|
| RICHARD,SANDRINE; SELLE,FRÉDÉRIC; LOTZ,JEAN-PIERRE; KHALIL,AHMED; GLIGOROV,JOSEPH; SOARES,DANIELE G.. |
| ABSTRACT It has now been 15 years since the HER2-targeted monoclonal antibody trastuzumab was introduced in clinical and revolutionized the treatment of HER2-positive breast cancer patients. Despite this achievement, most patients with HER2-positive metastatic breast cancer still show progression of their disease, highlighting the need for new therapies. The continuous interest in novel targeted agents led to the development of pertuzumab, the first in a new class of agents, the HER dimerization inhibitors. Pertuzumab is a novel recombinant humanized antibody directed against extracellular domain II of HER2 protein that is required for the heterodimerization of HER2 with other HER receptors, leading to the activation of downstream signalling pathways.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; Dimerization; HER2/3; Monoclonal antibody; Pertuzumab; Trastuzumab. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200565 |
| |
|
|
| COSTA-SILVA,DANYLO R.; BARROS-OLIVEIRA,MARIA DA CONCEIÇÃO; BORGES,RAFAEL S.; TAVARES,CLÉCITON B.; BORGES,UMBELINA S.; ALVES-RIBEIRO,FRANCISCO A.; SILVA,VLADIMIR C.; SILVA,BENEDITO B. DA. |
| ABSTRACT Insulin-like Growth Factor-1 (IGF-1) gene polymorphism has been associated with an increased risk for breast cancer. IGF-1 is a key regulator of proliferation, cell differentiation and apoptosis. It has important mitogenic and anti-apoptotic activities in normal cells and in breast cancer cells, acting synergistically with estrogen to increase neoplastic cell proliferation. This review aims to present the recent finds of IGF-1 gene polymorphism and its relationship with the risk of breast cancer through following the polymorphic dinucleotide repeat cytosine-adenine (CA) and single nucleotide polymorphisms (SNPs) by searching in the PubMed database publications focused studies published from 2010 to 2015 related to IGF-1 gene polymorphism and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breast cancer; Risk factors; IGF-1; IGF-1 gene polymorphism. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602349 |
| |
| Registros recuperados: 67 | |
|
|
|
