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| Registros recuperados: 28 | |
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| Bai,Xi; Yuan,Xianjun; Wen,Aiyou; Li,Junfeng; Bai,Yunfeng; Shao,Tao. |
| Background: Cold-active endo-1, 4-β-glucanase (EglC) can decrease energy costs and prevent product denaturation in biotechnological processes. However, the nature EglC from C. farmeri A1 showed very low activity (800 U/L). In an attempt to increase its expression level, C. farmeri EglC was expressed in Escherichia coli as an N-terminal fusion to protein S (ProS) from Myxococcus xanthus. Results: A novel expression vector, pET(ProS-EglC), was successfully constructed for the expression of C. farmeri EglC in E. coli. SDS-PAGE showed that the recombinant protein (ProS-EglC) was approximately 60 kDa. The activity of ProS-EglC was 12,400 U/L, which was considerably higher than that of the nature EglC (800 U/L). ProS-EglC was active at pH 6.5-pH 8.0,... |
| Tipo: Journal article |
Palavras-chave: Cellulose degradation; Cellulose; Cold-active enzyme; Endoglucanases; Enzymatic properties; Escherichia coli; Expression; Novel expression vector; N-terminal fusion; Protein S-tag; Recombinant protein. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600012 |
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| Rasmussen-Poblete,Susana; Valdes,Jorge; Gamboa,Maria Cecilia; Valenzuela,Pablo D.T; Krauskopf,Erwin. |
| Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that... |
| Tipo: Journal article |
Palavras-chave: Cellulose; Cold-temperature; EST database; Forest biotechnology; Lignin. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008 |
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| Valenzuela,Eduardo; Hipp,Joseline; Alonso,Claudia; Godoy,Roberto; Alberdi,Miren; Alvarez,Maricel; Saavedra,Isabel. |
| At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC.... |
| Tipo: Journal article |
Palavras-chave: Cellulose; Ectomycorrhizal fungi; Nothofagus; PH; Phosphatase; Temperature. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200012 |
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| Wang,Nengfei; Zang,Jiaye; Ming,Kaili; Liu,Yu; Wu,Zuohao; Ding,Hui. |
| Background: Cellulose can be converted to ethanol by simultaneous saccharification and fermentation (SSF). The difference between the optimal temperature of cellulase and microbial fermentation, however, has been identified as the critical problem with SSF. In this study, one fungal strain (AnsX1) with high cellulase activity at low temperature was isolated from Antarctic soils and identified as Verticillium sp. by morphological and molecular analyses. Results: The biochemical properties of crude AnsX1 cellulase samples were studied by filter paper cellulase assay. The maximum cellulase activity was achieved at low temperature in an acidic environment with addition of metal ions. Furthermore, AnsX1 cellulase demonstrated 54-63% enzymatic activity at... |
| Tipo: Journal article |
Palavras-chave: Antarctic soil; Cellulose; Filter paper cellulase assay; Psychrotrophic fungi; Verticillium sp. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400010 |
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| SALLES, H. O.; EGITO, A. S. do; CARNEIRO, J. da C.; XIMENES, L. V.; RIBEIRO, M. T.; RIBEIRO, R. P.; BARBOSA, I. C.; SOUSA, A. M. P.. |
| A criação do banco de extratos composto por enzimas fibrolíticas isoladas de conteúdo ruminal de bovinos, caprinos e ovinos, foi iniciativa da Embrapa Caprinos e Ovinos e da Embrapa Gado de Leite, através de projetos de pesquisa em parceria, e tem como objetivo salvaguardar extratos enzimáticos de nossa biodiversidade para utilização biotecnológica, visando caracterizar, avaliar e identificar possíveis aplicabilidades científicas e/ou industriais das enzimas contidas nesses extratos. |
| Tipo: Comunicado Técnico (INFOTECA-E) |
Palavras-chave: Bioetanol; Enzimas fibrolíticas; Sugar byproducts.; Cellulolysis; Etanol; Cana de açúcar; Bagaço; Celulase; Enzima celulolítica; Cellulose; Biodegradation; Sugarcane; Ethanol.. |
| Ano: 2017 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1087003 |
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| MELO, C. F. M. de; SOUZA, H. B. de; LOUREIRO, M. R. C.; WISNIEWSKI, A.. |
| Reunindo excelentes características, a madeira de Ucuúba (Virola surínamensis (Rol) Warb) tem sido extensivamente empregada na fabricação de laminados. Como consequêncía do processo produtivo, a madeira que não pode ser integralmente aproveitada, deixa um apreciável resíduo que, a falta de melhor emprêgo, não tem tido outra utilizaçãc senão como combustível na própria Usina. Estudando-se a ucuúba como fonte de celulose para papel, conclui-se que se trata, na verdade, de excelente matéria: prima, mesmo os resíduos de laminação que se constituem de restos de madeira de várias idades; Podem ser produzidos papéis Kraft de bôa qualidade, em ambos os casos, embora quando se trate da utilização dos resíduos de laminação as características de resistência ao... |
| Tipo: Séries anteriores (INFOTECA-E) |
Palavras-chave: Analysis; Fiber.; Análise; Celulose; Fibra; Madeira; Papel; Ucuuba; Tecnologia; Virola Surinamensis.; Cellulose; Kraft paper; Technology; Wood.. |
| Ano: 1971 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/376076 |
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| Silva,Rafael Lopes da; Araújo,Eric Portilho de; Rocha,Mariucha Karina Honório Ribeiro; Damasceno,Flavia Mota; Freitas,Jakeline Marcela Azambuja de; Sartori,Maria Márcia Pereira; Meirelles,Paulo Roberto de Lima; Barros,Margarida Maria; Pezzato,Luiz Edivaldo. |
| Abstract: The objective of this work was to evaluate the diet components - crude fiber, neutral detergent fiber, acid detergent fiber, hemicellulose, cellulose, and lignin - as internal indicators in the determination of the apparent digestibility coefficients of dry matter, gross energy, and of the nutrients - crude protein, phosphorus, and amino acids - in Nile tilapia (Oreochromis niloticus). Groups of ten juveniles (80.3±1 g) were randomly distributed in six tanks of 250 L and fed two practical diets, either of a plant-origin diet or of a plant- and animal-origin diet. Both diets were supplemented with 0.1% chromium (III) oxide (Cr2O3). Faeces were collected by the modified Guelph system. The apparent digestibility coefficients were determined by the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oreochromis niloticus; Cellulose; Fiber; Nutrients.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2017000600393 |
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| Carvalho,Isabela Pena Carvalho de; Detmann,Edenio; Mantovani,Hilário Cuquetto; Paulino,Mário Fonseca; Valadares Filho,Sebastião de Campos; Costa,Viviane Aparecida Carli; Gomes,Daiany Iris. |
| The objective of this study was to evaluate the effects of supplementation with different sources of energy and nitrogenous compounds on the in vitro growth and production of bacteriocin of lactic acid bacteria. Incubations were performed by using ruminal fluid from a rumen-fistulated Holstein-Zebu steer. The animal was kept on a Brachiaria decumbens pasture receiving 200 g/day of supplemental crude protein. Substrates and inoculum were placed in glass flasks considering eight treatments: cellulose, cellulose and casein, cellulose and soy peptone, cellulose and urea, starch, starch and casein, starch and soy peptone and starch and urea. Successive incubations were conducted to select microorganisms according to the energy sources and nitrogenous compounds.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacteriocin; Cellulose; Protein effect; Rumen microbiology. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000600014 |
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| Orrico,Ana Carolina Amorim; Centurion,Stanley Ribeiro; Farias,Romildo Marques de; Orrico Junior,Marco Antonio Previdelli; Garcia,Rodrigo Garófallo. |
| The objective was to evaluate the differences between distinct types of litter material and their combinations in the dynamics of degradation on the organic matter fractions and the quality of the final compound. The treatments were established according to material used as substrate for broiler litter: treatment 1 - rice husks; 2 - sugar cane bagasse; 3 - wood shavings; 4 - wood shavings + sugar cane bagasse; 5 - rice husks + sugar cane bagasse; and 6 - Napier grass. The following variables were monitored: temperature, levels of total solids (TS), volatile solids (VS), mass and volume of the pile, fibrous fraction, and levels and reductions of N, P and K during the process. Piles formed with Napier grass and sugar cane bagasse presented the highest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler production; Cellulose; Lignin; Temperature; Total solids. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000700028 |
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| Castro, George Meredite Cunha de; Benevides, Norma Maria Barros; Cabral, Maulori Curié; Miranda, Rafael de Souza; Gomes Filho, Enéas; Rocha, Maria Valderez Ponte; Araújo, Marjory Lima Holanda. |
| The seaweeds are bio-resource rich in sulfated and neutral polysaccharides. The tropical seaweed species used in this study (Solieria filiformis), after dried, shows 65.8% (w/w) carbohydrate, 9.6% (w/w) protein, 1.7% (w/w) lipid, 7.0% (w/w) moisture and 15.9% (w/w) ash. The dried seaweed was easily hydrolyzed under mild conditions (0.5 M sulfuric acid, 20 min.), generating fermentable monosaccharides with a maximum hydrolysis efficiency of 63.21%. Galactose and glucose present in the hydrolyzed were simultaneously fermented by Saccharomyces cerevisiae when the yeast was acclimated to galactose and cultivated in broth containing only galactose. The kinetic parameters of the fermentation of the seaweed hydrolyzed were Y(P⁄S) = 0.48 ± 0.02 g.g−1, PP = 0.27 ±... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ciências da Vida Bioquímica iota-carrageenan; Cellulose; Galactose; Kinetic parameters; Fermentation; Acclimation; Sulfuric acid. Biotecnologia. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37227 |
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| Registros recuperados: 28 | |
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