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Acute toxicity of essential oils of Aloysia triphylla (L'Hér.) Britton, Lippia gracilis Schauer, and Piper aduncum L. in Colossoma macropomum (Cuvier, 1818). Repositório Alice
SANTOS, P. R.; ANDRADE-PORTO, S. M.; OLIVEIRA, M. I. B.; BRANDÃO, F. R.; MATOS, L. V.; VELÁSQUEZ, J. G. R.; FARIAS, C. F. S.; CARPIO, K. C. R.; CHAVES, F. C. M.; CHAGAS, E. C..
Abstract: The aim of this study was to determine the acute toxicity of the essential oils (EOs) of Aloysia triphylla, Lippia gracilis and Piper aduncum in juvenile tambaqui (Colossoma macropomum), and evaluate the possible histopathological alterations in their gills. Resumo: O objetivo deste estudo foi determinar a toxicidade aguda dos óleos essenciais (OEs) de Aloysia triphylla, Lippia gracilis e Piper aduncum em juvenis de tambaqui (Colossoma macropomum), e avaliar as possíveis alterações histopatológicas em suas brânquias.
Tipo: Artigo de periódico Palavras-chave: Lethal concentration; Natural products; Produtos naturais; Tambaqui; Colossoma Macropomum; Óleo Essencial; Histologia; Histology.
Ano: 2023 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1157825
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Análise histológica do grão de pólen do jenipapeiro. Repositório Alice
FREIRE, g. DA s.; MACHADO, C. de A.; SILVA, A. V. C. da; LEDO, A. da S..
Tipo: Resumo em anais e proceedings Palavras-chave: Jenipapo; Fruta Tropical; Histologia Vegetal; Genética Vegetal; Tropical and subtropical fruits; Histology; Plant genetics.
Ano: 2023 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160030
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Oocyte development in pirarucu Arapaima gigas (Schinz, 1822). Repositório Alice
TORATI, L. S.; KIRSCHNIK, L. N. G.; LIMA, A. F.; MIGAUD, H..
This work aimed to describe the oocyte development in pirarucu, Arapaima gigas, from primary growth to ovulation stages for the first time.
Tipo: Anais e Proceedings de eventos Palavras-chave: Peixe; Pirarucu; Reprodução; Histologia Animal; Fish; Arapaima gigas; Histology; Reproduction; Oocytes.
Ano: 2021 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1134955
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The role of BAP in somatic embriogenesis induction from seed explants of Arachis species from Sections Erectoides and Procumbentes. Repositório Alice
PACHECO, G.; GAGLIARDI, R. F.; CARNEIRO, L. A.; CALLADO, C. H.; VALLS, J. F. M.; MANSUR, E..
Tipo: Separatas Palavras-chave: Groundnut; Somati embriogenesis; Microprogagação; Erectoides; Procumbentes; Amendoim; Histologia; Arachis; Histology; Micropropagation.
Ano: 2007 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/189262
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Cryopreservation of Coffea arabica L. zygotic embryos by vitrification. Repositório Alice
FREITAS, R. T. da; PAIVA, R.; SALES, T. S.; SILVA, D. P. C. da; REIS, M. V. dos; SOUZA, A. C. de; ROSA, S. D. V. F. da.
As a consequence of the difficulty in conventional coffee seed storage, biotechnological alternatives such as cryopreservation have been investigated. The objective of this study was to develop a protocol for the cryopreservation of Coffea arabica L. (cv. ?Catuaí Vermelho? - IAC 144) zygotic embryos by vitrification. For the cryopreservation study, the embryos were immersed in Plant Vitrification Solution 2 at different times (0, 10, 25, 50, 100, and 250 min) and two temperatures (0 and 25 °C). Subsequently, the best thawing time was determined in a water bath (1, 3, 5 minutes or directly in Recovery Solution). An anatomical study was conducted on non-stored and stored embryos, with or without the use of Plant Vitrification Solution 2. The immersion in...
Tipo: Artigo de periódico Palavras-chave: Liquid nitrogen; PVS2; TTC test; Ex situ conservation; Histology; Thawing.
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065188
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Dynamics of thermolysis and skin microstructure in water buffaloes reared in humid tropical climate: a microscopic and thermographic study. Repositório Alice
VILELA, R. A.; LOURENÇO JUNIOR, J. DE B.; JACINTO, M. A. C.; BARBOSA, A. V. C.; PANTOJA, M. H. DE A.; OLIVEIRA, C. M. C.; GARCIA, A. R..
The thermolytic capacity test is used to assess the adaptability of animals to existing environmental conditions. However, there is insufficient information on the relationship between histomorphometry and adaptability of buffaloes. Thus, this study aimed to assess the use of thermolysis pathways by buffaloes reared in a hot and humid environment so as to understand the relationships between environment, skin morphological characteristics, and heat storage, as well as the intensity and proportionality of use of its ways of dissipating heat to maintain homeothermy. The heat tolerance test, associated with the evaluations via infrared thermography, was applied to 10 female Murrah buffaloes and tegument histomorphometry was carried out. The animals exhibited...
Tipo: Artigo de periódico Palavras-chave: Acclimatization; Infrared thermography; Precision livestock farming; Bubalus Bubalis; Heat stress; Histology; Thermoregulation.
Ano: 2022 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1145185
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HISTOLOGICAL DESCRIPTION OF THE REPRODUCTIVE SYSTEM OF MALE AND FEMALE HATCHLINGS OF THE MAGDALENA RIVER TURTLE (Podocnemis lewyana) Acta biol.Colomb.
SÁNCHEZ-OSPINA,ANDRÉS C; RODRIGUEZ,BERARDO; CEBALLOS,CLAUDIA P..
In this study we described the macroscopic and microscopic histology of the reproductive system of male and female Podocnemis lewyana neonates (3.5 months old). We found macroscopic differences in the morphology of the gonads between the sexes, with ovaries being twice as long as testes, and testes being twice as wide as ovaries. Microscopically, we identified several immature elements, such as the lack of a muscle layer in the oviduct of females, and simple epithelia instead of pseudostratified epithelia in the oviduct and epididymis described in reptile adults. We also found a black pigment observed macroscopically in the mesovarium, and macroscopically and histologically in the epididymis. This pigment is consistent with the center of melano-macrophages...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epididymis; Histology; Ovary; Oviduct; Pigment; Testis.
Ano: 2014 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2014000300009
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POLYPHENOLS DISTRIBUTION AND RESERVE SUBSTANCES ANALYSIS IN CACAO SOMATIC EMBRYOGENESIS Acta biol.Colomb.
GALLEGO RÚA,Adriana María; HENAO RAMÍREZ,Ana María; URREA TRUJILLO,Aura Inés; ATEHORTÚA GARCÉS,Lucía.
In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Histology; Reserve accumulation; Recalcitrance; Somatic embryogenesis.
Ano: 2016 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000200002
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Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique Acta Botanica
Padilha,João Henrique Delfrate; Ribas,Luciana Lopes Fortes; Amano,Érika; Quoirin,Marguerite.
Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clusters were transferred to a conversion medium (plant growth regulator-free medium). Primary callus induction rate was higher in the first three TCLs and in media...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-iP; Callus induction; Histology; Macaw palm; Picloram.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400516
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How to construct and use a simple device to prevent the formation of precipitates when using Sudan Black B for histology Acta Botanica
Oliveira,João Marcelo Santos de.
The present work aims to demonstrate the stages of fabrication and use of a simple device to avoid the formation or fixation of precipitates from Sudan Black B solution on tissues. The device consists of four coverslip fragments attached to a histology slide, which serve as points of support for the histological slide under analysis. To work properly, the histology slide with the sections should be placed with the sections facing downwards the device. A small space between the device and the histology slide is thereby created by the height of the coverslip fragments. When Sudan Black B is applied, the solution is maintained within the edges of the device and evaporation is minimized by the small space, thereby reducing the consequent formation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histochemical tests; Histology; Lipids; Plant anatomy; Sudan Black B.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400489
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In Vitro Propagation of Heliconia bihai (L.) L. from Zygotic Embryos Acta Botanica
Ulisses,Cláudia; Melo-de-Pinna,Gladys Flávia; Willadino,Lilia; Albuquerque,Cynthia Cavalcanti de; Camara,Terezinha Rangel.
The internal morphology of embryos from immature and mature fruits of Heliconia bihai (L.) L. cv. Lobster Claw Two was examined. Embryos were inoculated into MS media (full MS and ½ MS) and GA3 (0, 2.5 and 5 mg L-1) with either sucrose or glucose. These plantlets were then replicated and transferred to MS medium (full MS or ½ MS) with 0 or 2.5 mg L-1 BAP and their multiplication was evaluated 30 and 45 days after inoculation. The genetic variability of the multiplied plants was estimated using isoenzyme analyses. The internal morphology of the mature embryos revealed their tissues to be in more advanced stages of differentiation than immature embryos. In the conversion phase, 85% of the inoculated embryos developed into plants in the ½ MS medium with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carbohydrates; Embryo culture; Histology; Isoenzymes; Micropropagation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000100018
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Morpho-anatomical characterization of embryogenic calluses from immature zygotic embryo of peach palm during somatic embryogenesis - DOI: 10.4025/actasciagron.v32i2.3248 Agronomy
Maciel, Simone Alencar; Universidade Federal do Acre; Fermino Junior, Paulo Cesar Poeta; Universidade Federal do Acre; Silva, Ricardo Alexandre; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Scherwinski-Pereira, Jonny Everson; Embrapa Recursos Genéticos e Biotecnologia.
The objective of this study was to morpho-anatomically characterize nodular embryogenic calluses from zygotic embryos of peach palm during the induction of somatic embryogenesis. Immature zygotic embryos were pre-treated in MS medium added to Picloram and 2,4-D (25 µM) and BAP (0, 5, 10 µM). After three months, primary calluses were transferred to MS induction medium added to Picloram and 2,4-D (450 µM). After six months, the embryogenic calluses were then histologically analyzed and cultivated in the maturation medium. The competent tissues of the zygotic embryos differentiated embryogenic calluses under action of both Picloram and 2,4-D auxins (450 µM), where the presence of multi-granular structures were observed....
Palavras-chave: Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos 5.00.00.00-4 Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos Ciências Agrárias.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3248
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Digestible methionine levels for white-egg layer pullets from 7 to 12 weeks of age Animal Sciences
Figueiredo Júnior, Jalceyr Pessoa; Costa, Fernando Guilherme Perazzo; Guerra, Ricardo Romão; Santana, Marcelo Helder Medeiros; Lima, Matheus Ramalho de; Pinheiro, Sarah Gomes.
An experiment was carried out to evaluate the requirement of digestible methionine for growing pullets at growth phase (7 to 12 weeks of age). A completely randomized design was distributed in five treatments, six replicates, and 15 pullets per experimental unit. 450 Dekalb White pullets from the 7th weeks of age, with an average initial weight of 313.14 ± 12.49 g were used. Dietary treatments consisted in five diets supplemented with DL-Methionine which resulted in five levels of digestible methionine (0.266, 0.294, 0.322, 0.350, and 0.378 %). Performance, serological blood, histopathology and histomorphometry data were evaluated. Quadratic responses were observed for final live weight (p < 0.0143), weight gain (p < 0.0073), feed conversion...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzymatic profile Essential amino acid Histology Morphometry. enzymatic profile; Essential amino acid; Histology; Morphometry..
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/47222
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Biochemical and cellularchanges in Oreochromis niloticus related to the water pollution of a degraded river - doi: 10.4025/actascibiolsci.v35i3.13207 Biological Sciences
Cruz, Zilma Maria Almeida; Universidade Vila Velha; Castro, Rodrigo Roque Lesqueves de; Universidade Vila Velha; Canal, Adriana; Azevedo Júnior, Romildo Rocha; Universidade Vila Velha; Silva, Ary Gomes da; Universidade Vila Velha; Ramos, Alessandro; Universidade Vila Velha.
The effects of polluted water at three sites in the Marinho River, Brazil, on Oreochromis niloticus (Nile tilápia) were investigated using histological, hematological and biochemical approaches. Fish exposed to the impacted water demonstrated that histological changes in gills were accompanied by nuclear and micronuclei abnormalities in cells. The activity of liver and plasma biomarkers (alkaline phosphatase (ALP), acid phosphatase (ACP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and liver glutathione S-transferase (GST)) showed an expressive change due to the. The results were also correlated with the highest levels of Cu+2, Zn+2 and Mn+2 in the water. The data of this study evidenced the importance of using a set of biomarkers to...
Palavras-chave: Aminotransferases; Phosphatases; Glutathione-S-transferase; Histology; Cell abnormality; Tilapia.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/13207
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Health State, morphological characterization and biometric parameters in the mangrove crab Ucides cordatus Linnaeus, 1763 (Brachyura: Ocypodidae) Biological Sciences
Valentim-Neto, Pedro Alexandre; Gesteira, Tereza Cristina Vasconcelos.
The crab Ucides cordatus is an important feature of mangroves in the North and Northeast of Brazil. In 2002 a large mortality was reported on this species in the Jaguaribe River Estuary. In order to investigate the possible causes specimens were collected monthly between May 2002 and December 2003 at five sites: Fortim (E1), Fortim (E2), Cumbe (E3) Aracati (E4) and Guajiru (E5). The mangrove structure and environmental parameters (salinity, pH, temperature and DO) were also evaluated. Water was collected for suspended solids analysis, BOD and nutrient levels. Salinity decreased in the rainy season, with no significant variation of pH and temperature. Dissolved oxygen levels had a mean of 5.7 mg L-1. Suspended solids increased at site E4, with BOD varying...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biometric parameters; Crustacea; Histology; Bioassay.
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47430
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Morphological and anatomical characterization of the digestive tract of Centropomus parallelus and Centropomus undecimalis - doi: 10.4025/actascibiolsci.v35i4.14352 Biological Sciences
Machado, Márcia Regina Fragoso; Universidade Nilton Lins; Souza, Helena de Oliveira; Universidade Federal do Rio de Janeiro; Souza, Valderes Lemos de; Fundação Oswaldo Cruz; Azevedo, Alexandre de; Universidade Federal do Rio de Janeiro; Goitein, Roberto; Instituto de Biociências da Univesidade Estadual Paulista (Unesp), Rio Claro – São Paulo; Nobre, André Dias; Universidade Nilton Lins.
Centropomus parallelus and Centropomus undecimalis are the most common species of Centropomidae in Brazil. Some authors attribute their failure to grow under in artificial conditions due to limited knowledge on the morphological and functional development of their digestive system and on nutrition requirements of both species. Current research characterizes histologically and histochemically the digestive tract of Centropomus parallelus and Centropomus undecimalis to provide a histophysiological assessment for studies related to their nutritional needs. In the esophagus, the mucous cells in the epithelium stained positively for PAS and AB. In the stomach, positive reactions to PAS and negative to AB respectively occurred on surface and mucous cells in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Digestive tract; Feeding habits; Histology; Centropomus.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14352
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Histological and immunohistochemical study of the thyroid gland of the broad-snouted caiman (Caiman latirostris) - doi: 10.4025/actascibiolsci.v35i4.18444 Biological Sciences
Machado-Santos, Clarice; Universidade Federal da Bahia.; Teixeira, Monalisa Jales; Médica Veterinária Autônoma; Sales, Armando; Universidade Federal Rural do Rio de Janeiro; Abidu-Figueiredo, Marcelo; Universidade Federal Rural do Rio de Janeiro.
The broad-snouted caiman, Caiman latirostris, is widely distributed in countries of South America. In Brazil it is considered an endangered species because of natural habitat destruction and illegal hunting.  In reptiles, the thyroid gland plays an integral part in ecdysis, reproduction, tail regeneration, growth, endocrine function and metabolism rate. The aim of the present study was to characterize the thyroid gland morphology of C. latirostris, based on histochemical and immunohistochemical techniques. The thyroid glands were fixed in Bouin's fluid and serial cross sections were stained with hematoxylin-eosin, Mallory’s trichrome, periodic acid-Schiff (PAS) and Alcian blue (AB pH 1.5 and 2.5). The immunohistochemical technique for 5-HT-IR cells was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências biológicas Thyroid; Histology; Reptiles; 5-HT Histologia.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18444
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Atrazine and nicosulfuron affect the reproductive fitness of the predator Podisus nigrispinus (Hemiptera: Pentatomidae) Anais da ABC (AABC)
REIS,TATIANE C.; SOARES,MARCUS A.; SANTOS,JOSÉ B. DOS; SANTOS,CONCEIÇÃO A. DOS; SERRÃO,JOSE EDUARDO; ZANUNCIO,JOSÉ C.; FERREIRA,EVANDER A..
Abstract Herbicides can impact non-target metabolic pathways in natural enemies and lead to the reduction of these populations in the field. Behavioral characteristics, morphology and histology of reproductive structures and reproduction of females of Podisus nigrispinus (Dallas) (Hemiptera: Pentatomidae) were evaluated under the effect of the herbicides atrazine, nicosulfuron and the mixture of both. The number of mature oocytes per ovary was lower in females exposed to the herbicides atrazine, nicosulfuron and the mixture of both. Herbicides did not affect the longevity and mortality of P. nigrispinus, therefore, they are selective for this predator. On the other hand, herbicides can cause sublethal effects by affecting the reproduction of predators.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Behavior; Histology; Morphology; Reproduction; Herbicides.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703625
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Reproductive morphology of female Guiana dolphins (Sotalia guianensis) Anais da ABC (AABC)
BECEGATO,ESTELLA Z.; ANDRADE,JANAINA P.; GUIMARÃES,JULIANA P.; VERGARA-PARENTE,JOCIERY E.; MIGLINO,MARIA ANGELICA; SILVA,FERNANDA M. DE OLIVEIRA E.
The reproductive morphology of cetaceans is poorly studied and, despite the large number of strandings, reports on this subject are scarce due to access to carcasses mostly in an advanced state of decomposition. The present study aimed to describe histological characteristics of the female genital tract ofSotalia guianensis, in order to assist in future studies on the reproductive biology of these animals. Females of different ages, from stranding events on beaches in northeastern Brazil, were used. Fragments of all organs were collected and processed for light and scanning electron microscopy. Histological analyses showed that these structures were similar to those found in terrestrial mammals, with some peculiarities, such as the presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Female genital system; Anatomy; Histology; Guiana dolphin.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401727
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Life-long Maternal Cafeteria Diet Promotes Tissue-Specific Morphological Changes in Male Offspring Adult Rats Anais da ABC (AABC)
SANTOS,CAROLYNE D.S.; BALBO,SANDRA L.; GUIMARÃES,ANA T.B.; SAGAE,SARA C.; NEGRETTI,FÁBIO; GRASSIOLLI,SABRINA.
ABSTRACT Here, we evaluated whether the exposure of rats to a cafeteria diet pre- and/or post-weaning, alters histological characteristics in the White Adipose Tissue (WAT), Brown Adipose Tissue (BAT), and liver of adult male offspring. Female Wistar rats were divided into Control (CTL; fed on standard rodent chow) and Cafeteria (CAF; fed with the cafeteria diet throughout life, including pregnancy and lactation). After birth, only male offspring (F1) were maintained and received the CTL or CAF diets; originating four experimental groups: CTL-CTLF1; CTL-CAFF1; CAF-CTLF1; CAF-CAFF1. Data of biometrics, metabolic parameters, liver, BAT and WAT histology were assessed and integrated using the Principal Component Analysis (PCA). According to PCA analysis worse...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Obesity; Dams; Offspring; Histology.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602887
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