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First report of Hop stunt viroid infecting Vitis gigas, V. flexuosa and Ampelopsis heterophylla. Repositório Alice
FAJARDO, T. V. M.; EIRAS, M.; NICKEL, O..
Hop stunt viroid (HSVd) is one of the most common viroids that infect grapevine ( Vitis spp.) worldwide. Sixteen sequences of the HSVd genome were obtained from infected grapevines in Brazil by next generation sequencing (NGS). Multiple alignments of the sequences showed nucleotide identities ranging from 94.6% to 100%. This is the first report of HSVd infecting two wild grape species and Ampelopsis heterophylla . These HSVd isolates along with others from V. vinifera and V. labrusca were phylogenetically analyzed. Keywords: Next generation sequencing . HSVd . Incidence . Genetic variability . Vitis
Tipo: Artigo de periódico Palavras-chave: Next generation sequencing; HSVd; Grapevine; Genetic variability; Vitis Labrusca; Vitis Vinifera; Incidence; Vitis; Hop stunt viroid; Viroids; Brazil.
Ano: 2018 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1088430
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Detection and molecular characterization of Grapevine yellow speckle viroid 1 isolates infecting grapevines in Brazil. Repositório Alice
FAJARDO, T. V. M.; EIRAS, M.; NICKEL, O..
Presently, Hop stunt viroid(HSVd) and Citrus exocortis viroid (CEVd) are the only viroids reported to infect grapevines (Vitis spp.) in Brazil, among the seven viroid species already reported infecting this host in other countries. All grapevine viroid diseases are graft-transmissible and can induce losses especially when associated with viruses. The aim of this work was to confirm infection by Grapevine yellow speckle viroid 1(GYSVd-1) in grapevine samples exhibiting yellow speckle symptoms in the leaves and in asymptomatic samples sequenced by next generation sequencing (NGS). The occurrence of this viroid in Brazil was further investigated in a second study. Total RNAs and dsRNAs were extracted from five symptomatic plants and 16 asymptomatic samples,...
Tipo: Artigo de periódico Palavras-chave: Next generation; Secondary structure; GYSVd-1; Vine.; Vinho; Incidence; Hop stunt viroid; Citrus exocortis viroid..
Ano: 2016 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1051941
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Non-2NS blast resistant wheat genotypes evaluated in the Brazilian Cerrado. Repositório Alice
WEBBER, N. W.; COELHO, M. A. de O; TORRES, G. A. M.; CECON, P. R.; CONSOLI, L.; DEUNER, C. C..
Abstract: Searching for novel sources of resistance to head blast is essential to strengthen wheat production in the Cerrado´s biome. The objective of this work was to evaluate disease intensity measures and yield for 2NS and non-2NS carriers wheat genotypes with varying heading times in Minas Gerais, Brazil. A total of fourteen wheat genotypes, two susceptible and twelve resistant to head blast, were sown in 2014, 2015, and 2017 at the Sertãozinho Experimental Station of Empresa de Pesquisa Agropecuária de Minas Gerais (Epamig) during three sowing dates without fungicide applications. The experimental design was a randomized complete block with three replicates. Blast incidence, severity, disease index and grain yield were influenced by the cycle of wheat...
Tipo: Artigo de periódico Palavras-chave: Productivity; Trigo; Genótipo; Doença de Planta; Fungo; Resistência; Produtividade; Triticum Aestivum; Magnaporthe oryzae; Incidence.
Ano: 2023 URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1155994
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Transmission of visceral leishmaniasis in dogs in a risk area of the metropolitan region of Belo Horizonte, Minas Gerais, Brazil Arq. Bras. Med. Vet. Zootec.
Lopes,E.G.P.; Oviedo-Pastrana,M.E.; Borges,L.F.N.M.; Freitas,A.C.P.; Dias,E.S.; Silva,S.R.; Haddad,J.P.A.; França-Silva,J.C.; Soares,D.F.M..
ABSTRACT Visceral leishmaniasis (VL) has spread rapidly across cities in the metropolitan region of Belo Horizonte. The aim of this study was to investigate VL dynamics in a prospective cohort study of dogs in Juatuba, between 2010 and 2011, to confirm the incidence of Leishmania infantum, and to assess possible risk factors associated with infection. An observational and prospective closed cohort study was performed using serology testing in dogs, randomly selected from the whole municipality. All seronegative dogs, or dogs with inconclusive results were monitored using indirect immunofluorescence (IIF) at 6-month intervals. The dog's owners completed a semi-structured questionnaire to assess possible causal factors of seroconversion, and the responses...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leishmaniasis; Incidence; Seroconversion; Risk factors; Cluster.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601403
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Epidemiology of Clostridium difficile: a hospital-based descriptive study in Argentina and Mexico BJID
Lopardo,Gustavo; Morfin-Otero,Rayo; Moran-Vazquez,Iliana Isabel; Noriega,Fernando; Zambrano,Betzana; Luxemburger,Christine; Foglia,Ginamarie; Rivas,Enrique Eduardo.
A prospective study was conducted in four tertiary hospitals in Argentina and Mexico in order to describe the occurrence of Clostridium difficileinfection (CDI) in these settings. The objective was to evaluate the incidence of CDI in at-risk populations in Argentina (one center) and Mexico (three centers) and to further explore potential study sites for vaccine development in this region. A prospective, descriptive, CDI surveillance study was conducted among hospitalized patients aged ≥40 years who had received ≥48 h of antibiotic treatment. Stool samples were collected from those with diarrhea within 30 days after starting antibiotics and analyzed for toxins A and B by ELISA, and positive samples were further tested by toxinogenic culture and restriction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium infections; Epidemiology; Latin America; Incidence.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100008
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A prospective observational cohort study to assess the incidence of acute otitis media among children 0-5 years of age in Southern Brazil BJID
Lanzieri,Tatiana M.; Cunha,Clóvis Arns da; Cunha,Rejane B.; Arguello,D. Fermin; Devadiga,Raghavendra; Sanchez,Nervo; Barria,Eduardo Ortega.
Abstract Objectives: To estimate acute otitis media incidence among young children and impact on quality of life of parents/caregivers in a southern Brazilian city. Methods: Prospective cohort study including children 0-5 years of age registered at a private pediatric practice. Acute otitis media episodes diagnosed by a pediatrician and impact on quality of life of parents/caregivers were assessed during a 12-month follow-up. Results: During September 2008-March 2010, of 1,136 children enrolled in the study, 1074 (95%) were followed: 55.0% were ≤2 years of age, 52.3% males, 94.7% white, and 69.2% had previously received pneumococcal vaccine in private clinics. Acute otitis media incidence per 1000 person-years was 95.7 (95% confidence interval:...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Acute otitis media; Children; Incidence; Quality of life.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000400468
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Disease burden of chronic hepatitis C in Brazil BJID
Ferreira,Paulo Roberto Abrão; Brandão-Mello,Carlos Eduardo; Estes,Chris; Gonçales Júnior,Fernando Lopes; Coelho,Henrique Sérgio Moraes; Razavi,Homie; Cheinquer,Hugo; Wolff,Fernando Herz; Ferraz,Maria Lúcia Gomes; Pessoa,Mário Guimarães; Mendes-Correa,Maria Cássia.
Background: Hepatitis C virus infection is a major cause of cirrhosis; hepatocellular carcinoma; and liver transplantation. The aim of this study was to estimate hepatitis C virus disease progression and the burden of disease from a nationwide perspective.Methods: Using a model developed to forecast hepatitis C virus disease progression and the number of cases at each stage of liver disease; hepatitis C virus-infected population and associated disease progression in Brazil were quantified. The impact of two different strategies was compared: higher sustained virological response and treatment eligibility rates (1) or higher diagnosis and treatment rates associated with increased sustained virological response rates (2).Results: The number of infected...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HCV; Disease burden; Epidemiology; Incidence; Brazil.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400363
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Hybrid capture as a tool for cervical lesions screening in HIV-infected women: insights from a Brazilian cohort BJID
Jalil,Emilia Moreira; Luz,Paula M.; Quintana,Marcel; Friedman,Ruth Khalili; Madeira,Rosa M. Domingues S.; Andrade,Angela Cristina; Chicarino,Janice; Moreira,Ronaldo Ismerio; Derrico,Monica; Levi,José Eduardo; Russomano,Fabio; Veloso,Valdilea Gonçalves; Grinsztejn,Beatriz.
ABSTRACT Introduction: Cervical cancer remains an important burden for HIV-infected women in the era of combination antiretroviral therapy. Recommendations for cervical screening in these women diverge and may include high-risk HPV (HRHPV) testing. We aimed to evaluate the clinical usefulness of a single HRHPV testing for cervical screening of HIV-infected women. Methods: 723 HIV-infected women from a Brazilian prospective cohort were included between 1996 and 2012. Inclusion criteria were: normal cervical cytology at baseline and having a HRHPV-test at baseline. We calculated incidence rates of any squamous intraepithelial lesion (SIL) and high grade SIL+ (HSIL+) and negative predictive values (NPV) within 12 and 36 months. Hazard Ratios were obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPV; HIV; Women; Cohort; Incidence.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100016
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The epidemiology of sepsis in a Brazilian teaching hospital BJID
Kauss,IAM; Grion,Cintia MC; Cardoso,LTQ; Anami,EHT; Nunes,LB; Ferreira,GL; Matsuo,T; Bonametti,AM.
OBJECTIVES: The objective of this study was to estimate disease incidence and mortality rate of sepsis in a tertiary public hospital. METHODS: Patients admitted to the Intensive Care Unit (ICU) in 2004 and 2005 were monitored for sepsis using an observational longitudinal study design. Patients were monitored daily for diagnostic criteria of sepsis, according to ACCP/SCCM Consensus Conference criteria, until either death or hospital discharge. RESULTS: During the study, we analyzed 1,179 patients. Systemic Inflammatory Response Syndrome (SIRS) was present in 1,048 (88.9%) patients on admission, and was associated with infection in 554 (47.0%) patients. Of these, sepsis was diagnosed in 30 (2.5%) patients, while severe sepsis was diagnosed in 269 (22.8%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sepsis; Incidence; Mortality; Epidemiology.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300011
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Trends in overall opportunistic illnesses, Pneumocystis carinii pneumonia, cerebral toxoplasmosis andMycobacterium avium complex incidence rates over the 30 years of the HIV epidemic: a systematic review BJID
Coelho,Lara; Veloso,Valdiléa Gonçalves; Grinsztejn,Beatriz; Luz,Paula Mendes.
BACKGROUND:The natural history of HIV infection has changed dramatically after the introduction of highly active antiretroviral therapy. Currently, opportunistic illnesses still represent a major cause of death and hospitalization in this population. In this study, we review the trends in opportunistic illnesses incidence rates and compare the results observed in high-income settings with that for low/middle-income settings, with special attention given to studies from Brazil.METHODS:We systematically searched Pubmed, Web of Science, Lilacs and Google scholar for publications on HIV associated opportunistic illness. Studies reporting rates based on person-time for all opportunistic illnesses and/or the three opportunistic infections of interest,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AIDS-related opportunistic infections; Opportunistic infection; Acquired immunodeficiency syndrome; Incidence.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200196
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Breast cancer in a cohort of human immunodeficiency virus (HIV)-infected women from Rio de Janeiro, Brazil: a cases series report and an incidence rate estimate BJID
Andrade,Angela Cristina Vasconcelos de; Luz,Paula Mendes; Veloso,Valdiléa Gonçalves; Cardoso,Sandra Wagner; Moreira,Ronaldo Ismerio; Grinsztejn,Beatriz; Friedman,Ruth Khalili.
Tipo: Info:eu-repo/semantics/report Palavras-chave: HIV; Breast neoplasms; Case reports; Incidence.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400016
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Transitioning through AIDS epidemics - gender and temporality BJID
Pinto,Clarice Souza; Fernandes,Carlos Eurico dos Santos; Oliveira,Roberto Dias de; Matos,Vanessa Terezinha Gubert de; Castro,Ana Rita Coimbra Motta de.
ABSTRACT OBJECTIVE: To understand the evolution of AIDS over time in Mato Grosso do Sul, Brazil, using incidence, lethality, and mortality coefficients. METHODS: A descriptive epidemiological study based on time series analysis of secondary data from the Notifiable Diseases Information System reported between 1985 and 2012. RESULTS: The prevalence of AIDS was higher among men, with evidence of feminization during the first 14 years of the epidemic. There was no statistically significant sex difference in AIDS lethality. Women were 1.3 times more likely to survive than men. CONCLUSIONS: Gender differences must be considered when designing new HIV/AIDS prevention strategies.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Aids; Mortality; Lethality; Incidence.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600657
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Occurrence and molecular characterization of Cucumber green mottle mosaic virus in cucurbit crops of KPK, Pakistan BJM
Ali,Asad; Hussain,Adil; Ahmad,Musharaf.
Field survey of the cucurbit crops revealed a high incidence of Cucumber green mottle mosaic virus (CGMMV) in Khyber Pakhtunkhwa Province (KPK), Pakistan. Among the seven districts surveyed, average percent incidence of CGMMV was recorded up to 58.1% in district Nowshera, followed by 51.1% in district Charsada, 40.5% in district Swabi and 37.3% in district Mardan. In Swat and Dir districts average incidence CGMMV was recorded upto 31.2% and 29.4%, respectively. Among the different crops highest incidence in plain areas of KPK was recorded in bottle gourd (59.3%) followed by 56.3% in Squash, 54.5% in Pumpkin, 45.5% in Melon, 41.7% in Cucumber and 29.9% in Sponge gourd. In Northern hilly areas highest incidence of CGMMV (52.9%) was observed in pumpkin,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cucurbit; CGMMV; Incidence; Tobamovirus; Pakistan.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400015
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Ethylene inhibitor aminoethoxyvinilglycine on glomerella leaf spot in apple cultivar 'Royal Gala' Ciência Rural
Bogo,Amauri; Casa,Ricardo Trezzi; Rufato,Leo; Kuhnem Júnior,Paulo Roberto; Gonçalves,Mayra Juline.
Aqueous solution of Aminoethoxyvinylglycine (AVG) has been commercialized in Brazil as ReTainTM C, 15% mainly as a potent inhibitor of ethylene biosynthesis by prevention of pre-harvest abscission and ripening of apple fruits. The effect of the product was evaluated during the 2007-08 and 2008-09 crop seasons in edafoclimatic conditions of Santa Catarina upland. Plants of 'Royal Gala' cultivar were sprayed with ReTainTM C, four weeks before the first commercial harvest at doses of 0; 62,5; 125, and 250mg a.i L-1. The incidence and severity were quantified weekly in 100 leaves distributed in four branches with 12 replications and assessed the area under the incidence -I and severity -S disease progress curve (AUIDPC and AUSDPC) of Glomerella leaf spot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Malus domestica; Growth regulator; Colletotrichum gloeoporioides; Incidence; Severity; Area under disease progress curve.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000600001
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Fluctuación poblacional de Brevipalpus phoenicis (Acari: tenuipalpidae), vector de la leprosis de los cítricos en Huimanguillo, Tabasco. Colegio de Postgraduados
Méndez Méndez, Prisciliano.
Se determinó la fluctuación poblacional del ácaro Brevipalpus phoenicis mediante muestreos quincenales de hojas, de octubre de 2009 a septiembre de 2010, en una plantación de 1 ha de naranja Valencia (Citrus sinensis) en la zona citrícola del estado de Tabasco, localizada en el municipio de Huimanguillo (17°42'00" Norte, 93°28'26" Oeste). Asimismo, se aplicó el análisis de correlación de Pearson entre dicha fluctuación y los datos de temperatura y precipitación obtenidos de una estación meteorológica localizada en la misma zona de estudio. El ácaro se presentó durante los 12 meses considerados, con mayor densidad de población en octubre y noviembre de 2009 y de abril a septiembre de 2010. La población presentó un descenso bien marcado que inició en...
Palavras-chave: Citrus sinensis; Acaro; Incidencia; Sureste de México; Mite; Incidence; Southeast of Mexico; Producción Agroalimentaria en el Trópico; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/1694
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Efectividad biológica de insecticidas contra el psílido de la papa (Bactericera cockerelli Sulc.) en Metepec Estado de México y transmisión de bacterias no cultivadas asociadas a enfermedades en papa (Solanum tuberosum L.) Colegio de Postgraduados
López Duran, María Felipa.
Con el propósito de identificar insecticidas efectivos para el manejo del psílido de la papa Bactericera cockerelli Sulc., y reducir la incidencia de la Punta Morada de la Papa (PMP), se realizó un experimento en Metepec, Edo. de México, donde se evaluaron siete insecticidas de diferentes grupos toxicológicos. Los mejores insecticidas para el control de huevos y adultos del psílido fueron extracto de nim y abamectina con 69.8 y 84.8% de efectividad biológica, mientras que para ninfas fueron spiromesifen, bifentrina, abamectina y flufenoxuron con 77.7% de efectividad. Sin embargo, ningún producto fue efectivo para reducir la incidencia de la Punta Morada de la Papa. El objetivo del segundo estudio fue determinar si B. cockerelli Sulc. transmite el...
Tipo: Tesis Palavras-chave: Candidatus Liberibacter; Control químico; Fitoplasmas; Incidencia; Infección; Inoculación; Paratrioza; Psílido; Punta morada de la papa; Severidad; Transmisión; Maestría; Entomología y Acarología; Chemical control; Phytoplasmas; Incidence; Infection; Inoculation; Paratrioza; Potato Purple Top; Psyllid; Severity; Transmission..
Ano: 2009 URL: http://hdl.handle.net/10521/1356
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Identificación, dispersión, histopatología e impacto económico de Armillaria en aguacate en Charapan, Michoacán. Colegio de Postgraduados
Michua Cedillo, Jeny.
La identificación, dispersión e impacto económico de Armillaria sp. en aguacate Hass se estudió en el municipio de Charapan, Michoacán. Armillaria se aisló de raíces de árboles sintomáticos y muertos y se cultivó en BDS. La identificación de la especie se realizó por compatibilidad somática y amplificación de la región IGS-I del ADN ribosomal de 19 aislamientos. Los apareamientos dieron como resultado un solo grupo de compatibilidad; los confrontamientos se realizaron con A. mellea, A. gallica y A. mexicana, donde se observó la separación de las colonias. Las pruebas de patogenicidad se realizaron inoculando 35 plántulas de aguacate en condiciones de invernadero. Las raíces de las plántulas infectadas y de los árboles infectados naturalmente en campo se...
Palavras-chave: Compatibilidad somática; Dispersión; Fenoles; IGS; Incidencia; Inoculación; Rizomorfos; Somatic compatibility; Dispersion; Phenols; Incidence; Inoculation; Rhizomorphs; Fitopatología; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2218
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Efecto del viroide de la mancha de sol en el rendimiento y calidad de frutos en aguacate de Michoacán. Colegio de Postgraduados
Saucedo Carabez, José Ramón.
Avocado sunblotch viroid (ASBVd) es una enfermedad considerada de importancia económica que se reportó en México en 2009. El objetivo del presente trabajo fue evaluar el efecto del ASBVd en el rendimiento y calidad de frutos en aguacate de Michoacán debido a la importancia de la enfermedad y del cultivo. Cuatro árboles sanos fueron comparados con cuatro asintomáticos y cuatro sintomáticos del cultivar ‘Hass’ y de la selección ‘Méndez’ en 2 huertos con diferente manejo. En campo se registró el rendimiento total de los árboles. En cada árbol se midió el peso, diámetro ecuatorial (DE), diámetro polar (DP) de 100 frutos y la incidencia de frutos con síntomas. Frutos ‘Hass’ de cinco condiciones: Frutos sanos de árboles sanos (C1), frutos sanos de árboles...
Palavras-chave: ASBVd; Efecto; Rendimiento; Calidad; Pérdida; Incidencia; Effect; Yield; Quality; Loss; Incidence; Fitopatología; Maestría.
Ano: 2013 URL: http://hdl.handle.net/10521/1977
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Antracnosis del fruto de litchi (Litchi chinensis Soon.) : etiología y control in vitro. Colegio de Postgraduados
Martínez Bolaños, Misael.
El litchi (Litchi chinensis Soon) es un fruto subtropical. El cultivar Brewster se explota comercialmente en la región del Papaloapan y el cv. Mauritius en los Bajos Mixes, Oaxaca, México. El manchado del pericarpio es uno de los problemas principales en pre y postcosecha del fruto en ambos cultivares. No se han encontrado registros de su causa o importancia en México, por ello los objetivos de esta investigación fueron: determinar la etiología del manchado café del pericarpio e iniciar la exploración del control in vitro de su agente causal. La antracnosis o manchado del pericarpio del litchi se caracterizó externa e internamente por lesiones circulares de color café, con márgenes bien definidos en el pericarpio, pero sin afectar el arilo o la semilla....
Palavras-chave: Brewster; Mauritus; Pathogenicity; Incidence; PCR; Patogenicidad; Incidencia; Antracnosis; Benomil; Procloraz; Hidróxido de cobre; Doctorado; Fitopatología.
Ano: 2012 URL: http://hdl.handle.net/10521/1701
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Biología del tizón suizo y su relación con el silicio y la nutrición de árboles de navidad. Colegio de Postgraduados
Salgado Feregrino, Oscar Javier.
En México, el Tizón Suizo, Phaeocryptopus gaeumannii, reduce la calidad y producción de árboles de navidad, poco se conoce del ciclo biológico. Esta investigación se dividió en tres partes. La primera tuvo como objetivo conocer la biología del hongo a través de un año, para ello se identificó, secuenció, evaluó incidencia, severidad, defoliación, porcentaje de aislamientos, ascosporas en pseudotecios y aerotransportadas. En la segunda parte se estudió in vitro la relación del hongo con el silicio y nutrimentos, se registró peso húmedo y seco. En la tercera parte se estudio in situ la relación con la dosis nutritiva y evaluó porcentaje de defoliación y concentración nutrimental en acículas. En la primera parte se encontró hasta tres periodos de incremento...
Palavras-chave: Forestal; Maestría; Ascosporas aerotransportadas; Incidencia; Phaeocryptopus gaeumannii; Severidad; Pseudotsuga menziesii; Airborne ascospore; Incidence; Severity.
Ano: 2013 URL: http://hdl.handle.net/10521/2075
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