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| RITTERBUSCH, G. A.; ESTEVES, P. A.; TREVISOL, I. M.; OKINO, C. H.; JAENISCH, F. R. F.; MORES, M. A. Z.; CARON, L.; SILVA, A. D.; FINGER, P. F.; HUBNER, S. O.. |
| A vacinação é a forma mais utilizada para prevenir a bronquite infecciosa causada pelo vírus da bronquite infecciosa das galinhas (IBV). Contudo, as vacinas convencionais são incapazes de diferenciar aves infectadas de vacinadas. No presente trabalho foi construído, caracterizado, e avaliado como candidato vacinal, um adenovírus recombinante expressando o gene N do IBV. O gene N foi clonado em um adenovírus humano tipo 5 defectivo e transfectado para as células HEK-293A para gerar rAd5_N. Após o vetor ser obtido como esperado e a confirmação da expressão da proteína N em HEK-293ª, foi realizada inoculação pela via oculo-nasal na dose de 107 TCID50/0,1mL para imunização de galinhas livres de patógenos específicos (SPF). A resposta imunológica do Ad5_N e a... |
| Tipo: Artigo de periódico |
Palavras-chave: Nucleoproteína; Bronquite; Adenovírus; Imunização; Vacinação; Adenoviridae; Infectious bronchitis; Nucleocapsid proteins; Immune response; Vaccination. |
| Ano: 2019 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114269 |
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| OKINO, C. H.; MORES, M. A. Z.; TREVISOL, I. M.; COLDEBELLA, A.; MONTASSIER, H. J.; BRENTANO, L.. |
| Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related... |
| Tipo: Artigo de periódico |
Palavras-chave: Bronquite; Frango; Bronchitis; Infectious bronchitis; Infectious bronchitis virus; Chickens. |
| Ano: 2017 |
URL: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609 |
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| Souza,C.M.; Rocha,F.R.T.; Martins,N.R.S.; Resende,J.S.; Jorge,M.A.; Rampinelli,A.P.. |
| Murine hybridomas producing IgG1 monoclonal antibodies (Mabs) against N and S2 proteins (53KDa and 82KDa, respectively) from avian infection bronchitis virus (IBV) strain M41 were generated by the fusion of a myeloma cell line (Sp2/0-Ag14) with spleen cells from Balb/c mice previously immunized with whole virus IBV M41. Post-fusion screening criterion was by ELISA and 36 positive hybrids were generated after fusions. Two hybrids specific to N (N3F10) and S2 (S12B2) proteins from M41 (serotype Massachusetts) were selected by western blotting. These Mabs recognized the Ark-99 (serotype Arkansas) and A5968 (serotype Connecticut) IBV strains in addition to M41. By ELISA, the Mab against the S2 (S12B2) recognized all reference and Brazilian strains (M41, SE-17,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; Monoclonal antibody; Infectious bronchitis; Nucleoprotein; Spike glicoprotein S2. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500002 |
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| Jones,RC. |
| The emergence and nature of different strains of infectious bronchitis virus (IBV) in Europe are described. Infectious bronchitis (IB) is the most important endemic viral respiratory disease where highly pathogenic Newcastle disease and avian influenza are not present. IB was first described in the UK in 1948 and identified as Massachusetts type. In the 1970s and 80s new serotypes were reported in Holland and elsewhere and new vaccines were developed. The 1990s saw the emergence of the major variant commonly called 793B, again needing a new vaccine. Two novel types have been recognised since 2000, Italy 02 and QX. Italy 02 appears to be well controlled by the use of two different live vaccines (H120 and the 793B-related 4/91) while for QX, associated with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Control; Chickens; Europe; Infectious bronchitis; Vaccine. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200009 |
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| Rauber,RH; Flôres,ML; Pereira,CE; Grigulo,M. |
| The present work was carried out to compare Infectious Bronchitis Virus (IBV) antibody titers in serum and egg yolk samples from laying hens. Sixty paired blood and egg samples were collected from laying hens of two farms. Serum samples were frozen, while egg yolk samples were diluted (1:500) before freezing. Serum and yolk samples were tested for the presence of IBV antibodies by indirect ELISA (commercial kit) and titers were compared by a correlation test (alpha=0.05). There was a high correlation (r=0.62) between the two kinds of samples, which means that titers of IBV antibodies in the egg yolk and in serum samples are quite the same. Considering that blood collection causes deep stress that leads to economic losses, and since eggs are collected daily... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibody; Egg yolk; ELISA; Infectious bronchitis; Serology. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000200008 |
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