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Registros recuperados: 36
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Effects of a native and a non-native macrophyte species of Hydrocharitaceae on Chironomidae and Oligochaeta assemblages structure doi: 10.4025/actascibiolsci.v35i3.10855 Biological Sciences
Behrend, Rômulo Diego de Lima; Universidade Estadual de Maringá; Teixeira, Mariana Carolina; Universidade Estadual de Maringá; Fernandes, Sue Ellen Prata; Universidade Estadual de Maringá; Camargo, Janielly Carvalho; Universidade Estadual de Maringá; Rosin, Gisele Cristina; Universidade Estadual de Maringá; Takeda, Alice Michiyo; Universidade Estadual de Maringá.
This study investigated the structure of the Oligochaeta and Chironomidae assemblages associated with monospecific stands of two submerged macrophyte species: Egeria najas and Hydrilla verticillata. Samplings were carried out in Leopoldo Backwater and Paraná river, in August 2008. To assess the structure of Oligochaeta and Chironomidae assemblage in each macrophyte we calculated: species density, richness, diversity, and evenness. A detrended correspondence analysis (DCA) was used to summarize Oligochaeta and Chironomidae assemblages composition. The highest values of species density and richness for the two groups were recorded in H. verticillata. Although there were no significant differences in density, richness and Shannon diversity, the assemblage...
Tipo: Distribuição espacial Palavras-chave: Ecology upper Paraná river; Submerged macrophytes; Hydrilla verticillata; Egeria najas; Invasion; Naididae Ecology.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10855
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Apoptosis in HEp-2 cells infected with Ureaplasma diversum Biol. Res.
Amorim,Aline Teixeira; Marques,Lucas Miranda; Santos,Angelita Maria Oliveira Gusmão; Martins,Hellen Braga; Barbosa,Maysa Santos; Rezende,Izadora Souza; Andrade,Ewerton Ferraz; Campos,Guilherme Barreto; Lobão,Tássia Neves; Cortez,Beatriz Araujo; Monezi,Telma Alvez; Machado-Santelli,Glaucia Maria; Timenetsky,Jorge.
BACKGROUND: Bacterial pathogens have many strategies for infecting and persisting in host cells. Adhesion, invasion and intracellular life are important features in the biology of mollicutes. The intracellular location ofUreaplasma diversum may trigger disturbances in the host cell. This includes activation or inhibition of pro and anti-apoptotic factors, which facilitate the development of host damage. The aim of the present study was to associate U. diversum infection in HEp-2 cells and apoptosis induction. Cells were infected for 72hs with four U. diversum clinical isolates and an ATCC strain. The U. diversuminvasion was analyzed by Confocal Laser Scanning Microscopy and gentamicin invasion assay. The apoptosis was evaluated using pro-apoptotic and...
Tipo: Journal article Palavras-chave: Ureaplasma diversum; Invasion; Apoptosis; HEp-2 cells.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100038
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Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line Biol. Res.
Buchegger,Kurt; Ili,Carmen; Riquelme,Ismael; Letelier,Pablo; Corvalán,Alejandro H.; Brebi,Priscilla; Huang,Tim Hui-Ming; Roa,Juan Carlos.
BACKGROUND: Reprimo (RPRM), a highly glycosylated protein, is a new downstream effector of p53-induced cell cycle arrest at the G2/M checkpoint, and a putative tumor suppressor gene frequently silenced via methylation of its promoter region in several malignances. The aim of this study was to characterize the epigenetic inactivation and its biological function in BC cell lines. METHODS: The correlation between RPRM methylation and loss of mRNA expression was assessed in six breast cancer cell lines by methylation specific PCR (MSP), 5'-Aza-2'-deoxycytidine treatment and RT-PCR assays. MDA-MB-231 cells were chosen to investigate the phenotypic effect of RPRM in cell proliferation, cell cycle, cell death, cell migration and invasion. RESULTS: In the cancer...
Tipo: Journal article Palavras-chave: Reprimo; MDA-MB-231; Migration; Invasion.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100005
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Role of regenerating gene IA expression on local invasion and survival in nasopharyngeal carcinoma Biol. Res.
Xing,Haijie; Chen,Xiangdong; Han,Yaofeng.
Abstract Background Regenerating gene IA (REGIA) plays an important role in tissue regeneration and tumors prognosis of epithelium origin. However, the role of REGIA in nasopharyngeal carcinoma (NPC) is unclear. This study aims to investigate the expression and function of REG1A in NPC. Results We have found that there was 63 patients with REGIA positive expression of 155 patients in this study (40.65%). The positive expression rate of REGIA was 30.50, 44.44 and 47.83% in stage T2, T3 and T4 patients, respectively. The REGIA expression was significantly difference in T2 and T4 stage tumors or T2 and T3–T4 stage. The positive expression rate of REGIA was found to be higher in patients with cervical lymph node persistence than those with cervical...
Tipo: Journal article Palavras-chave: Nasopharyngeal carcinoma; Regenerating gene IA; Invasion; Survival.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100227
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Ichthyofauna of headwater streams from the rio Ribeira de Iguape basin, at the boundaries of the Ponta Grossa Arch, Paraná, Brazil Biota Neotropica
Frota,Augusto; Message,Hugo José; Oliveira,Rachel Calil de; Benedito,Evanilde; Graça,Weferson Júnio da.
Abstract: The rio Ribeira de Iguape basin (Paraná State) is highly relevant for the preservation of remnants of the Atlantic Forest. Its high levels of diversity and fish endemism explain the river dynamics promoted by the Ponta Grossa Arch, a geological structure that promotes headwater capture and isolation between the upper rio Paraná drainage, the rio Iguaçu and the rio Ribeira de Iguape. Here, our objective was to provide an unprecedented inventory for headwater streams of the rio Ribeira de Iguape basin at the boundaries of the Ponta Grossa Arch. We found 29 species of fish representing four orders and nine families. Siluriformes was the richest order followed by Characiformes. Nine species presented high abundance of sampled individuals,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Altitudinal gradient; Biogeography; Coastal drainages; Ichthyology; Invasion.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032019000100307
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Cantharidin suppressed breast cancer MDA-MB-231 cell growth and migration by inhibiting MAPK signaling pathway BJMBR
Gu,X.-D.; Xu,L.-L; Zhao,H.; Gu,J.-Z; Xie,X.-H.
As an active constituent of the beetle Mylabris used in traditional Chinese medicine, cantharidin is a potent and selective inhibitor of protein phosphatase 2A (PP2A) that plays a crucial role in cell cycle progression, apoptosis, and cell fate. The role and possible mechanisms exerted by cantharidin in cell growth and metastasis of breast cancer were investigated in this study. Cantharidin was found to inhibit cell viability and clonogenic potential in a time- and dose-dependent manner. Cell cycle analysis revealed that cell percentage in G2/M phase decreased, whereas cells in S and G1 phases progressively accumulated with the increasing doses of cantharidin treatment. In a xenograft model of breast cancer, cantharidin inhibited tumor growth in a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cantharidin; Breast cancer; ERK/MAPK pathway; Growth; Migration; Invasion.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000700608
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Relationship between matrix metalloproteinases and the occurrence and development of ovarian cancer BJMBR
Zhang,Y.; Chen,Q..
Ovarian cancer is one of the most malignant genital cancers, with a high mortality rate. Many researchers have suggested that matrix metalloproteinases (MMPs) have remarkably high expression in ovarian cancer tissues. MMPs are considered to be related to the occurrence, development, invasion and metastasis of ovarian cancer. Moreover, some studies have discovered that the unbalance between MMPs and tissue inhibitor of metalloproteinases (TIMPs) are associated with the malignant phenotype of tumors. This review summarizes the latest research progress of MMPs in ovarian cancer. The investigation of MMP mechanism in ovarian cancer will facilitate the development of effective anti-tumor drugs, and thereby improve the survival rate of patients with ovarian...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovarian cancer; Matrix metalloproteinases (MMPs); Tissue inhibitor of metalloproteinases (TIMPs); Invasion; Metastasis.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000600301
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Interleukin-27 augments the inhibitory effects of sorafenib on bladder cancer cells BJMBR
Cao,J.Y.; Yin,H.S.; Li,H.S.; Yu,X.Q.; Han,X..
Both sorafenib and interleukin-27 (IL-27) are antineoplastic drugs. This study aimed to investigate the synergistic effect of these two drugs on bladder cancer cells. HTB-9 and T24 cells were stimulated with IL-27 (50 ng/mL), sorafenib (2 μM) or the synergistic action of these two drugs. The cells without treatment acted as control. Cell proliferation, apoptosis and invasion were measured by bromodeoxyuridine assay, flow cytometry and modified Boyden chamber, respectively. Simultaneously, both modified Boyden chamber and scratch assay were used to assess cell migration. Finally, the phosphorylation levels of key kinases in the Akt/mechanistic target of rapamycin (mTOR)/mitogen-activated protein kinase (MAPK) pathway, and expression levels of matrix...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sorafenib; Interleukin 27; Proliferation; Apoptosis; Invasion; Akt/mTOR/MAPK.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000800610
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Downregulation of survivin by siRNA inhibits invasion and promotes apoptosis in neuroblastoma SH-SY5Y cells BJMBR
Zhang,L.; Liang,H.; Cao,W.; Xu,R.; Ju,X.L..
Neuroblastoma is a solid tumor that occurs mainly in children. Malignant neuroblastomas have a poor prognosis because conventional chemotherapeutic agents are not very effective. Survivin, a member of the inhibitor of the apoptosis protein family, plays a significant role in cell division, inhibition of apoptosis, and promotion of cell proliferation and invasion. Previous studies found that survivin is highly expressed in some malignant neuroblastomas and is correlated with poor prognosis. The aim of this study was to investigate whether survivin could serve as a potential therapeutic target of human neuroblastoma. We employed RNA interference to reduce survivin expression in the human neuroblastoma SH-SY5Y cell line and analyzed the effect of RNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Neuroblastoma; Survivin; Apoptosis; Invasion; RNA interference.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000700548
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Propofol inhibits invasion and growth of ovarian cancer cells via regulating miR-9/NF-κB signal BJMBR
Huang,X.; Teng,Y.; Yang,H.; Ma,J..
Propofol is one of the most commonly used intravenous anesthetic agents during cancer resection surgery. A previous study has found that propofol can inhibit invasion and induce apoptosis of ovarian cancer cells. However, the underlying mechanisms are not known. miR-9 has been reported to be little expressed in ovarian cancer cells, which has been related to a poor prognosis in patients with ovarian cancer. Studies have also demonstrated that propofol could induce microRNAs expression and suppress NF-κB activation in some situations. In the present study, we assessed whether propofol inhibits invasion and induces apoptosis of ovarian cancer cells by miR-9/NF-κB signaling. Ovarian cancer ES-2 cells were transfected with anti-miR-9 or p65 cDNA or p65 siRNA...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Propofol; Ovarian cancer; MiR-9; NF-κB; Apoptosis; Invasion.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016001200605
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Adipose tissue-derived stem cells promote pancreatic cancer cell proliferation and invasion BJMBR
Ji,S.Q.; Cao,J.; Zhang,Q.Y.; Li,Y.Y.; Yan,Y.Q.; Yu,F.X..
To explore the effects of adipose tissue-derived stem cells (ADSCs) on the proliferation and invasion of pancreatic cancer cells in vitroand the possible mechanism involved, ADSCs were cocultured with pancreatic cancer cells, and a cell counting kit (CCK-8) was used to detect the proliferation of pancreatic cancer cells. ELISA was used to determine the concentration of stromal cell-derived factor-1 (SDF-1) in the supernatants. RT-PCR was performed to detect the expression of the chemokine receptor CXCR4 in pancreatic cancer cells and ADSCs. An in vitro invasion assay was used to measure invasion of pancreatic cancer cells. SDF-1 was detected in the supernatants of ADSCs, but not in pancreatic cancer cells. Higher CXCR4 mRNA levels were detected in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adipose tissue-derived stem cells; Pancreatic cancer; Proliferation; Invasion; SDF-1.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900758
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Expression of CHPF modulates cell proliferation and invasion in lung cancer BJMBR
Cao,Chengsong; Liu,Yong; Wang,Qun; Zhao,Jing; Shi,Ming; Zheng,Junnian.
Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CHPF; Lung cancer; Proliferation; Invasion; TCGA; Affymetrix Gene Chip.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500610
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Distribution of virulence genes sefC, pefA and spvC in Salmonella Enteritidis phage type 4 strains isolated in Brazil BJM
Castilla,Karina Salvagni; Ferreira,Claudete Serrano Astolfi; Moreno,Andrea Micke; Nunes,Iolanda Aparecida; Ferreira,Antônio José Piantino.
The distribution of virulence genes, sefC, pefA and spvC, was investigated in 110 Salmonella Enteritidis phage type 4 strains by polymerase chain reaction. Their influence in the caecal colonization and invasion of liver and spleen of one-day-old chickens was studied. Eight isolates were negative for the spvC gene, three for the pefA gene and one, for the sefC gene. These results allowed grouping the strains into four genotypes. Presence of these genes did not influence bacteria invasion in the liver and spleen of the chickens ten days after infection, although the presence of more than one fimbrial gene can be related to caecal colonization.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Virulence genes; Colonization; Invasion.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200007
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Adhesive and invasive capacities of Edwarsiella tarda isolated from South American sea lion BJM
Fernández,Araceli; Paz Villanueva,María; González,Mario; Fernández,Fabiola; Latif,Fadua; Flores,Sandra Nonier; Fernández,Heriberto.
Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Edwarsiella tarda; Adherence; Invasion; Otaria flavescens.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300044
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Adhesion and invasion of Clostridium perfringens type A into epithelial cells BJM
Llanco,Luis A.; Nakano,Viviane; Moraes,Claudia T.P. de; Piazza,Roxane M.F.; Avila-Campos,Mario J..
ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Invasion; Clostridium perfringens type A; Necrotic enteritis; TpeL gene.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400764
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Invasiveness of Cut-Leaf Ground-Cherry (Physalis angulata L.) Populations and Impact of Soil Water and Nutrient Availability Chilean J. Agric. Res.
Travlos,Ilias S.
Biological invasions are a major threat to natural ecosystems and agroecosystems, while weed flora is noticeably changing globally. In this study we evaluated the potential of cut-leaf ground-cherry (Physalis angulata L.), a species native to America, to invade the semi-arid regions of Greece. Greenhouse and laboratory experiments were conducted to evaluate the effects of different environmental resources (nutrient and water availability) on seedling growth, biomass production, fecundity, and seed germination of four populations of cut-leaf ground-cherry. Our results suggest that cut-leaf ground-cherry does not tolerate extreme drought during the first growth stages, while it can survive and produce adequate and rapidly germinated seed (> 85%) under low...
Tipo: Journal article Palavras-chave: Physalis angulata; Invasion; Greece; Water stress.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392012000300009
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A preliminary analysis of death cause, capture-related mortality, and survival of adult red deer in northwestern Patagonia Ecología austral
Flueck,Werner T; Smith-Flueck,Joanne M; Bonino,Never A.
The red deer (Cervus elaphus), among the world´s 14 most invasive exotic mammals, has recently arrived in Patagonia. Forty-seven deer were captured, marked with radio collars, and monitored in order to determine survival rates and identify causes of death. Net gunning from a helicopter allowed captures to be evenly distributed through the study area and in a timely manner. The absence of capture related mortality in our study agrees with previously reported low rates for this method. Animals were monitored for periods ranging from 5 to 2611 days. Ten animals provided censored values due to radio failures or being shot: they survived on average 811 days (SE = 221) after capture. Ten animals experienced natural deaths with an average time alive after capture...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Invasion; Cervus elaphus; Puma concolor; Predation; Disease; Radio telemetry; Population dynamics; Capture by net gunning.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1667-782X2005000100004
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The Effects of Urban Sprawl on Birds at Multiple Levels of Biological Organization Ecology and Society
Blair, Robert; University of Minnesota; blairrb@umn.edu.
Urban sprawl affects the environment in myriad ways and at multiple levels of biological organization. In this paper I explore the effects of sprawl on native bird communities by comparing the occurrence of birds along gradients of urban land use in southwestern Ohio and northern California and by examining patterns at the individual, species, community, landscape, and continental levels. I do this by assessing the distribution and abundance of all bird species occupying sites of differing land-use intensity in Ohio and California. Additionally, I conducted predation experiments using artificial nests, tracked the nest fate of American Robins and Northern Cardinals, and assessed land cover in these sites. At the individual level, predation on artificial...
Tipo: Peer-Reviewed Reports Palavras-chave: Avian community; California; Cardinalis cardinalis; Extinction; Faunal homogenization; Invasion; Landscape heterogeneity; Nesting success; Ohio; Predation; Turdus migratorius; Urbanization.
Ano: 2004
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Novelty, Adaptive Capacity, and Resilience Ecology and Society
Allen, Craig R; U.S. Geological Survey, Nebraska Cooperative Fish and Wildlife Research Unit; allencr@unl.edu; Holling, C. S.; Department of Zoology, University of Florida; holling@zoo.ufl.edu.
We present a conceptual framework that explores some of the forces creating innovation and novelty in complex systems. Understanding the sources of variability and novelty may help us better understand complex systems. Understanding complex phenomena such as invasions, migration, and nomadism may provide insight into the structure of ecosystems and other complex systems, and aid our attempts to cope with and mitigate these phenomena, in the case of invasions, and better understand and or predict them. Our model is broadly applicable to ecological theory, including community ecology, resilience, restoration, and policy. Characterizing the link between landscape change and the composition of species communities may help policymakers in their decision-making...
Tipo: Peer-Reviewed Insight Palavras-chave: Adaptation; Cross-scale; Extinction; Innovation; Invasion; Speciation.
Ano: 2010
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Weed Control as a Rationale for Restoration: The Example of Tallgrass Prairie Ecology and Society
Blumenthal, Dana M; University of Minnesota; dblumenthal@npa.ars.usda.gov; Jordan, Nicholas R; University of Minnesota; perr0128@tc.umn.edu; Svenson, Elizabeth L; University of Minnesota; esvenso1@swarthmore.edu.
The potential weed control benefits of ecological restoration are rarely cited and largely unstudied. Nevertheless, the nature of many restoration target communities, i.e., diverse, late-successional communities, suggests that restoration may control weeds and that the invasibility of plant communities may decrease with both diversity and successional age. Given the high cost of weed control in nonagricultural land, weed control benefits could be a strong incentive for restoration efforts. We examined the cumulative effects of restoration on weed populations 7 yr after tallgrass prairie restoration on a Minnesota sand plain. The numbers and biomass of volunteer weeds were compared among randomized plots with (1) no restoration, (2) prairie seed addition,...
Tipo: Peer-Reviewed Reports Palavras-chave: Community invasibility; Invasion; Prairie; Restoration; Succession; Weed competition; Weed control; Weeds.
Ano: 2003
Registros recuperados: 36
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