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| Registros recuperados: 41 | |
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| Diniz,Michely C.; Costa,Marcília P.; Pacheco,Ana C.L.; Kamimura,Michel T.; Silva,Samara C.; Carneiro,Laura D.G.; Sousa,Ana P.L.; Soares,Carlos E.A.; Souza,Celeste S.F.; Oliveira,Diana Magalhães de. |
| Several motile processes are responsible for the movement of proteins into and within the flagellar membrane, but little is known about the process by which specific proteins (either actin-associated or not) are targeted to protozoan flagellar membranes. Actin is a major cytoskeleton protein, while polymerization and depolymerization of parasite actin and actin-interacting proteins (AIPs) during both processes of motility and host cell entry might be key events for successful infection. For a better understanding the eukaryotic flagellar dynamics, we have surveyed genomes, transcriptomes and proteomes of pathogenic Leishmania spp. to identify pertinent genes/proteins and to build in silico models to properly address their putative roles in trypanosomatid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Actin-interacting proteins (AIPs); Flagellar proteins; Leishmania; Coronin and Arp2/3; Phagosome. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300033 |
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| Pinto,Erika G.; Tempone,Andre G.. |
| Abstract Background: Considering the high toxicity and limited therapies available for treating visceral leishmaniasis (VL), the drug repositioning approach represents a faster way to deliver new therapies to the market. Methods: In this study, we described for the first time the activity of a potent antiarrhythmic, amiodarone (AMD), against L. (L.)infantum and its in vitro and in vivo activity. Results: The evaluation against promastigotes has shown that amiodarone presents leishmanicidal effect against the extracellular form, with an IC50 value of 10 μM. The activity was even greater against amastigotes in comparison with promastigotes with an IC50 value of 0.5 μM. The selectivity index in relation to the intracellular form demonstrated that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Visceral leishmaniasis; Amiodarone; In vitro; In vivo. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100320 |
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| Soares,M. J. V.; Moraes,J. R. E.; Palmeira Borges,V.; Miyazato,L. G.; Moraes,F. R.. |
| Visceral leishmaniasis (VL) is a zoonosis that affects both animals and man. Dogs are the etiological agents main reservoir. The aim of this study was to evaluate the clinical laboratory aspects and renal histopathology of VL dogs. Thirty-four symptomatic (case) and 17 asymptomatic (control) VL seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Diagnosis was confirmed by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Clinical and laboratory tests included blood cell count and renal function analysis (urea and creatinine). Animals were subjected to euthanasia and necropsy. Renal fragments were prepared by the usual histological techniques and stained with hematoxylin-eosin and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dogs; Kala-azar; Leishmania; Renal lesion. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400014 |
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| Camargo,L. B; Langoni,H.. |
| Leishmaniasis is a parasitic zoonosis caused by protozoans of the genus Leishmania transmitted by insects known as phlebotomines, which are found in wild or urban environments. It affects domestic and wild animals and transmission to man happens by accident. The disease occurs in tropical and sub-tropical areas, mainly in Asia, Europe, Africa, and the Americas. There are two forms that affect man: American cutaneous leishmaniasis (ACL) and American visceral leishmaniasis (AVL). The latter is caused by three species of Leishmania: Leishmania (Leishmania) donovani, Leishmania (Leishmania) infantum, and Leishmania (Leishmania) chagasi, which are grouped in the Leishmania (Leishmania) donovani complex. Wild reservoir hosts of L. chagasi known so far are foxes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Cutaneous leishmaniasis; Visceral leishmaniasis; Leishmania. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400002 |
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| Soares,M. J. V.; Moraes,J. R. E.; Roselino,A. M. F.. |
| In human and canine renal histological studies of visceral leishmaniasis (VL), the etiological agent is rarely found in situ. The objective of this study was to evaluate PCR in identifying the etiological agent in spleen, liver, lymph node, and kidneys of VL-seropositive dogs. Twenty-five symptomatic (case group) and 15 asymptomatic (control group) VL-seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Serologic diagnosis was made by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Animals were subjected to euthanasia and necropsy. Renal fragments were immersed in buffered formaldehyde solution. Spleen, liver, lymph node, and kidney samples were collected and frozen at -70ºC until DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dogs; Leishmania; PCR; Kidney. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400011 |
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| Voltarelli,EM; Arraes,SMAA; Perles,; Lonardoni,MVC; Teodoro,U; Silveira,TGV. |
| Leishmania sp. infection was investigated in wild animals from the Ingá Park, in the municipality of Maringá, Paraná state, Brazil, where American cutaneous leishmaniasis (ACL) is an endemic disease. Sixty-five mammals, comprising Didelphis albiventris, Cerdocyon thous, Lycalopex vetulus, Cebus apella, Dasyprocta azarae, Dasypus novemcinctus, Procyon cancrivorus and Nasua nasua, were captured. Blood samples were collected for parasite cultivation. Antibodies were investigated by direct agglutination test (DAT) using Leishmania (Viannia) braziliensis as antigen. Flagellates were observed in blood cultures of 14 (35.9%) Didelphis albiventris. Anti-Leishmania antibodies were detected in 31 (51.6%) specimens of Cerdocyon thous, Lycalopex vetulus, Cebus apella,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Wild animals; Reservoir; Agglutination tests. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400011 |
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| Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C.. |
| Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR). |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012 |
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| Faiões,Viviane dos Santos; Frota,Lívia C. R. M. da; Cunha-Junior,Edézio Ferreira; Barcellos,Julio C. F.; Silva,Thayssa Da; Daher Netto,Chaquip; Da-Silva,Silvia Amaral Gonçalves; Silva,Alcides J. M. da; Costa,Paulo R. R.; Torres-Santos,Eduardo Caio. |
| Abstract Background: Despite the development of new therapies for leishmaniasis, among the 200 countries or territories reporting to the WHO, 87 were identified as endemic for Tegumentary Leishmaniasis and 75 as endemic for Visceral Leishmaniasis. The identification of antileishmanial drug candidates is essential to fill the drug discovery pipeline for leishmaniasis. In the hit molecule LQB-118 selected, the first generation of pterocarpanquinones was effective and safe against experimental visceral and cutaneous leishmaniasis via oral delivery. In this paper, we report the synthesis and antileishmanial activity of the second generation of pterocarpanoquinones. Methods: The second generation of pterocarpanquinones 2a-f was prepared through a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Pterocarpanquinone; LQ-118; Phenotypic assay; Leishmaniasis; Drug discovery; Neglected diseases. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100325 |
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| Pereira,Andreia Vieira; Barros,Gustavo de; Pinto,Erika Gracielle; Tempone,Andre Gustavo; Orsi,Ricardo de Oliveira; Santos,Lucilene Delazari dos; Calvi,Sueli; Ferreira Jr,Rui Seabra; Pimenta,Daniel Carvalho; Barraviera,Benedito. |
| Abstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results Promastigotes and intracellular amastigotes were susceptible to melittin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Melittin; Apis mellifera; Leishmania; Leishmaniasis; Peptides; Toxins; Antiparasitic; Cytokines. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301 |
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| COMANDOLLI-WYREPKOWSKI,Claudia Dantas; GRAFOVA,Iryna; NAIFF,Maricleide de Farias; AVELLA,Maurizio; GENTILE,Gennaro; GRAFOV,Andriy; FRANCO,Antonia Maria Ramos. |
| ABSTRACT Current treatment of cutaneous leishmaniasis (CL) relies mainly on pentavalent antimonials salts and second-line drugs include pentamidine and amphotericin B, but these therapies have side effects and require parenteral administration. The aim of this work was to evaluate the topical formulations containing pentamidine isethionate (PI) in the experimental treatment of cutaneous leishmaniasis (CL). Golden hamsters (Mesocricetus auratus) were infected in the nose with Leishmania (Leishmania) amazonensis. Six treatment groups received different topical treatments of anhydrous or hydrating emulsions, for a maximum of 10 days, with an application of 50 mg day-1. After treatment tissue samples of lesions were evaluated by histology, transmission... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Topical formulations; Experimental treatment; Pentamidine isethionate; Animal model; Leishmania. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000100039 |
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| Reis,Sônia Rolim; Gomes,Luis Henrique Monteiro; Ferreira,Natália Mota; Nery,Liliane da Rocha; Pinheiro,Francimeire Gomes; Figueira,Luanda de Paula; Soares,Fabiane Veloso; Franco,Antonia Maria Ramos. |
| A leishmaniose é uma antropozoonose com focos naturais, nos quais a infecção é mantida em ecótopos silvestres entre a população de vetores e seus hospedeiros vertebrados. Neste estudo foram identificadas espécies de flebotomíneos envolvidas na transmissão de leishmaniose tegumentar no Bairro Cidade de Deus, onde há focos da doença humana e animal. O bairro está situado à margem da reserva florestal Adolpho Ducke. Foram capturados 122 espécimens em épocas variadas, utilizando-se armadilhas luminosas do tipo CDC. As espécies de flebotomíneos mais encontradas foram Lutzomyia umbratilis, L. rorotaensis e L. olmeca nociva. Nas proximidades de uma das residências onde foram realizadas as coletas de flebotomíneos, verificou-se descarte de resíduos orgânicos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manaus; Leishmania; Vetores. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672013000100016 |
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| GOENAGA-MAFUD,Loraine Carolina; EYES-ESCALANTE,Melisa; FLOREZ-ARRI ETA,Fernando. |
| RESUMEN Dentro del género Lutzomyia algunas especies son vectores de la enfermedad llamada leishmaniasis, la cual tiene implicaciones en la salud pública, desarrollando diferentes manifestaciones clínicas como leishmaniasis cutánea, visceral y mucocutánea que afectan altamente al ser humano. El objetivo de este estudio fue realizar un inventario de las especies del género Lutzomyia presentes en seis zonas del departamento del Atlántico. Se implementaron tres técnicas de capturas tradicionales: trampas CDC entre 18:00-06:00, trampa Shannon entre 19:00-22:00 y búsqueda activa de flebotomíneos con aspiradores bucales en troncos, bases de árboles y otros sitios entre 09:00-11:00 y 16:00-17:30, en seis municipios del departamento del Atlántico, Colombia. Se... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Costa Caribe colombiana; Entomología; Insectos vectores; Leishmania; Phlebotomus. |
| Ano: 2020 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000300284 |
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| Sobreira Dantas Nóbrega de Figuêiredo,Francisco Rodolpho; Gyllyandeson,de Araújo Delmondes; Paulo da Cruz,Luzia; Amaro do Nascimento,Érika; Relison Tintino,Saulo; Santiago Lemos,Izabel Cristina; Bezerra da Cunha,Francisco Assis; Vega Gomez,Maria Celeste; Coronel,Cathia; Martins da Costa,José Galberto; Melo Coutinho,Henrique Douglas; Bezerra Felipe,Cícero Francisco; Alencar de Menezes,Irwin Rose; Kerntopf,Marta Regina. |
| As doenças parasitárias, também chamadas de “doenças negligenciadas”, continuam sendo uma grande dificuldade para o desenvolvimento social e econômico dos países mais pobres. Podemos citar como exemplo dessas doenças, a leishmaniose e a doença de Chagas. A leishmaniose é causada por parasitas do gênero Leishmania e afeta cerca de 12 milhões de pessoas. A doença de Chagas, causada pelo protozoário Trypanosoma cruzi, causa aproximadamente 50.000 mortes por ano. Os fármacos disponíveis para o tratamento dessas doenças são altamente tóxicos, sendo este um dos motivos que leva à busca por drogas eficazes e seguras para seus tratamentos. As folhas da Annona squamosa, espécie da família Annonaceae, já foram descritas na literatura por suas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Annona squamosa; Annonaceae; Leishmania; Trypanosoma cruzi. |
| Ano: 2016 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432016000300001 |
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| MELO NETO,BERNARDO; LEITÃO,JOSEANA M.S.R.; OLIVEIRA,LUCIANO G.C.; SANTOS,SÉRGIO E.M.; CARNEIRO,SABRINA M.P.; RODRIGUES,KLINGER A.F.; CHAVES,MARIANA H.; ARCANJO,DANIEL D.R.; CARVALHO,FERNANDO A.A.. |
| ABSTRACT Zanthoxylum rhoifolium Lam. (Rutaceae) has been traditionally used in the treatment of microbial infections and parasitic diseases. In the present study, the antileishmanial effect induced by the ethanol extract of stem barks from Z. rhoifolium (ZR-EEtOH) and its n-hexane fraction (ZR-FHEX) on infection and infectivity of murine macrophages by promastigote forms of Leishmania amazonensis were investigated. In different set of experiments, macrophages or promastigotes were pretreated with ZR-EEtOH or ZR-FHEX at non-lethal concentrations for 24 hours, and then macrophages were submitted to infection by promastigotes. Moreover, their effects on activation of macrophages, as well as on the DNA content, size and number of promastigotes by flow... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Macrophages; Nitric oxide; Promastigote; Phagocytic activity; Zanthoxylum. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000501851 |
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| Registros recuperados: 41 | |
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