|
|
|
| Registros recuperados: 47 | |
|
| |
|
| |
|
|
| Yang,Yanhua; Dai,Li; Xia,Hengchuan; Zhu,Keming; Liu,Haijun; Chen,Keping. |
| Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4-7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mass spectrometry; Proteomic analysis; Rice seed; Two-dimensional electrophoresis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100012 |
| |
|
| |
|
| |
|
|
| BARIZON, R. R. M.; FERRACINI, V. L.; QUEIROZ, S. C. do N. de; SOUZA, M. D. de; SPADOTTO, C. A.; SOUZA, D. R. C. de. |
| Resumo - Este trabalho teve como objetivo realizar testes de campo e de laboratório, utilizando os princípios das boas práticas de laboratório (BPL) e boas práticas agrícolas (BPA). Esses princípios foram criados para harmonizar procedimentos e garantir a aceitação dos resultados em diferentes países e regiões da Organização para Cooperação e Desenvolvimento Econômico (OCDE), além de serem obrigatórios para registro de agrotóxicos. Para adequar à instalação de teste desses princípios foi realizado um estudo de campo com aplicação de permetrina no cultivo de pepino, que é considerado uma cultura com suporte fitossanitário insuficiente (ou minor crops). Este pesticida foi selecionado por duas razões: (a) vem sendo detectado nos programas de monitoramento, e... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Boas práticas de laboratório; Boas práticas agrícolas; Permetrina; GC-MS/MS; Espectrometria de massas; Agrotóxico; Espectrometria; Análise Química; Pepino; Cucumis; Pesticides; Good laboratory practices; Good agricultural practices; Mass spectrometry. |
| Ano: 2019 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1111785 |
| |
|
|
| ROSA, J. S. da; FREITAS-SILVA, O.; CAMPOS, R. da S.; CASTRO, I. M. de; TEIXEIRA, A. da S.. |
| O café é a segunda maior commodity do mundo e tamanha importância faz do café uma matriz alimentar muito estudada. A necessidade de incremento na produtividade dessa cultura, porém, pode aumentar o uso de alguns agrotóxicos em sistemas de produção de café. Da mesma forma, as práticas de colheita e processamento pós-colheita do café estão diretamente relacionadas à contaminação dos grãos por fungos micotoxigênicos. Rastrear a presença destes dois tipos de contaminantes em amostras de café Arábica comum (com diferentes teores de grãos defeituosos) e submetidos a diferentes níveis de torra foi o objetivo deste trabalho. Os agrotóxicos foram analisados pelo método multirresíduo, por espectrometria de massas sequencial, e a ocratoxina A, por cromatografia... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Contaminante; Cromatografia líquida de alta eficiência; Micotoxina; Coffea Arábica; Tecnologia de Alimento; Espectrometria; Cromatografia; Food technology; Vegetable products; High performance liquid chromatography; Mycotoxins; Mass spectrometry; Poisoning; Agrochemicals. |
| Ano: 2019 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1112112 |
| |
|
|
| Dubois, Nolwenn; Barthomeuf, Chantal; Berge, Jean-pascal. |
| It is essential to have simple rapid methods for the determination of fatty acid structures. Traditionally, fatty acids are analysed by gas chromatography using their methyl ester derivatives (FAME). However, their corresponding mass spectra exhibit molecular ions but are usually devoid of ions indicative of structural features and, notably, the position of double bounds on the aliphatic chains [1]. In the most useful approach to structure determination, the carboxyl group is derivatised with a reagent containing a nitrogen atom. Recently, a convenient method for preparing picolinyl esters from intact lipids has been published [2]. However, some problems occurred in our laboratory when this method was used, leading to some modifications and optimisation.... |
| Tipo: Text |
Palavras-chave: Sensitivity; Sterol esters; Fatty acid methyl esters; Gas chromatography; Mass spectrometry; Picolinyl esters. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2125.pdf |
| |
|
|
| Ferreira Araujo, Daniel; Machado, Wilson; Weiss, Dominik; Mulholland, Daniel S.; Garnier, Jeremie; Souto-oliveira, Carlos E.; Babinski, Marly. |
| Recent work has shown that variations in zinc (Zn) isotope ratios enable us to identify contamination sources in the terrestrial environment and uptake processes in higher plants. Here in this study, we demonstrate that this also holds true for mangrove forests, which play an important role in the biogeochemical cycling of metals in tropical coastal ecosystems and that are seriously threatened by anthropogenic pollution. To this end, we determined zinc concentration and isotope composition (expressed using the δ66Zn notation relative to the JMC 3-0749-L standard) in sediments and tree leaves collected from a mangrove close to Rio de Janeiro in Brazil. The δ66ZnJMC values of sediments vary between +0.36 and + 0.84‰ and fall on a mixing line between detrital... |
| Tipo: Text |
Palavras-chave: Metal transition isotopes; Metal contamination; Mass spectrometry; Coastal ecosystems; Mangrove leaves. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00440/55196/56660.pdf |
| |
|
| |
|
|
| Cacas, Jean-luc; Bure, Corinne; Furt, Fabienne; Maalouf, Jean-paul; Badoc, Alain; Cluzet, Stéphanie; Schmitter, Jean-marie; Antajan, Elvire; Mongrand, Sébastien. |
| Although Glycosyl-Inositol-Phospho-Ceramides (GIPCs) are the main sphingolipids of plant tissues, they remain poorly characterized in term of structures. This lack of information, notably with regard to polar heads, currently hampers the understanding of GIPC functions in biological systems. This situation prompted us to undertake a large scale-analysis of plant GIPCs: 23 plant species chosen in various phylogenetic groups were surveyed for their total GIPC content. GIPCs were extracted and their polar heads were characterized by negative ion MALDI and ESI mass spectrometry. Our data shed light on an unexpected broad diversity of GIPC distributions within Plantae, and the occurrence of yet-unreported GIPC structures in green and red algae. In monocots,... |
| Tipo: Text |
Palavras-chave: Glycosphingolipid; Glycosyl-Inositol-Phospho-Ceramide; GIPC; Mass spectrometry; MALDI-TOF; Polar head; Roots; Photosynthetic tissues; Berries. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00166/27689/25884.pdf |
| |
|
|
| Gonthier, Isabelle; Rager, Marie-noëlle; Metzger, Pierre; Guezennec, Jean; Largeau, Claude. |
| The lipids of a deep-sea hydrothermal vent arch ae on, Thermococcus S 557, were isolated, purified and structurally determined. Based on acid methanolysis and spectroscopic studies, the polar lipids were shown to comprise diphytanyl glycerol and dibiphytanyl diglycerol, typical membrane lipids of Arch aea. From the neutral lipids, 2,3-di-O-dihydro-14,15-geranylgeranyl glycerol was isolated. This novel ether lipid is, very likely, a close intermediate in the biosynthesis of diphytanyl glycerol diether in Arch aea. |
| Tipo: Text |
Palavras-chave: Mass spectrometry; NMR; Novel glycerol diether lipid; Thermococcus sp; Archaea. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-736.pdf |
| |
|
| |
|
|
| Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier. |
| In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with... |
| Tipo: Text |
Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf |
| |
|
|
| Kilcoyne, Jane; Twiner, Michael J.; Mccarron, Pearse; Crain, Sheila; Giddings, Sabrina D.; Foley, Barry; Rise, Frode; Hess, Philipp; Willdns, Alistair L.; Miles, Christopher O.. |
| Azaspiracids (AZAs) are marine biotoxins produced by dinoflagellates that can accumulate in shellfish, which if consumed can lead to poisoning events. AZA7–10, 7–10, were isolated from shellfish and their structures, previously proposed on the basis of only LC–MS/MS data, were confirmed by NMR spectroscopy. Purified AZA4–6, 4–6, and 7–10 were accurately quantitated by qNMR and used to assay cytotoxicity with Jurkat T lymphocyte cells for the first time. LC–MS(MS) molar response studies performed using isocratic and gradient elution in both selected ion monitoring and selected reaction monitoring modes showed that responses for the analogues ranged from 0.3 to 1.2 relative to AZA1, 1. All AZA analogues tested were cytotoxic to Jurkat T lymphocyte cells in a... |
| Tipo: Text |
Palavras-chave: Azaspiracid; Structure confirmation; LC-MS molar response; NMR; Mass spectrometry; Purification; Jurkat T; Toxicity. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/38059/36190.pdf |
| |
|
|
| Mann, Florian; Szczerbowski, Daiane; De Silva, Lisa; Mcclure, Melanie; Elias, Marianne; Schulz, Stefan. |
| Male ithomiine butterflies (Nymphalidae: Danainae) have hairpencils on the forewings (i.e. androconia) that disseminate semiochemicals during courtship. While most ithomiines are known to contain derivatives of pyrrolizidine alkaloids, dihydropyrrolizines or g-lactones in these androconia, here we report on a new class of fatty acid esters identified in two subspecies, Ithomia salapia aquinia and I. s. derasa. The major components were identified as isoprenyl (3-methyl-3-butenyl) (Z)-3-acetyloxy-11-octadecenoate, isoprenyl (Z)-3-acetyloxy-13-octadecenoate (12) and isoprenyl 3-acetyloxyoctadecanoate (11) by GC/MS- and GC/IR-analyses, microderivatizations, and synthesis of representative compounds. The absolute configuration of 12 was determined to be R. The... |
| Tipo: Text |
Palavras-chave: Fatty acid esters; Mass spectrometry; Mimicry; Pheromones; Pyrrolizidine alkaloids. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75512/76362.pdf |
| |
|
|
| Zykwinska, Agata; Berre, Ludivine Tripon-le; Sinquin, Corinne; Ropartz, David; Rogniaux, Helene; Colliec-jouault, Sylvia; Delbarre-ladrat, Christine. |
| Polysaccharides have attracted much attention due to their interesting physico-chemical and also biological properties that are explored in food, cosmetic and pharmaceutical industries. GY785 exopolysaccharide (EPS) presenting an unusual structure is secreted by the deep-sea hydrothermal bacterium, Alteromonas infernus. Low-molecular weight (LMW) derivatives obtained by chemical depolymerization of the native high molecular weight (HMW) EPS were previously shown to exhibit biological properties similar to glycosaminoglycans (GAG). In the present study, in order to generate well defined derivatives with a better control of the depolymerization, an enzymatic approach was applied for the first time. Various commercially available enzymes were firstly screened... |
| Tipo: Text |
Palavras-chave: Exopolysaccharide; Enzyme; Depolymerization; Oligosaccharide; Mass spectrometry. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53458/54352.pdf |
| |
|
|
| Bernay, Benoît; Baudy-floc'H, Michele; Zanuttini, Bruno; Zatylny, Céline; Pouvreau, Stephane; Henry, Joël. |
| For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in... |
| Tipo: Text |
Palavras-chave: Mass spectrometry; Myotropin; APGWamide; Egg laying; Molluscan. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1697.pdf |
| |
|
|
| Hess, Philipp. |
| An overview is given of the biological origin of phycotoxins, as well as their chemical characteristics. Major poisoning types are described and examples of poisoning events are given to illustrate the importance of the phenomenon to both shellfish consumers and the shellfish producing industry. The characteristics of phycotoxins as natural products, the lack of predictability of their occurrence, economic drivers and the freshness of shellfish consumed in many countries result in a number of requirements for methods to be used in the efficient detection of these compounds. Subsequently, the performance of mouse bioassays and mass spectrometry as detection tools are compared for examples from Irish and French monitoring programmes to assess the usefulness... |
| Tipo: Text |
Palavras-chave: Bioanalytical methods; Bioassays; Mass spectrometry; Liquid chromatography; Biological samples. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11767/8989.pdf |
| |
|
|
| Ben Amor, Faten; Elleuch, Fatma; Ben Hlima, Hajer; Garnier, Matthieu; Saint-jean, Bruno; Barkallah, Mohamed; Pichon, Chantal; Abdelkafi, Slim; Fendri, Imen. |
| The green microalgae Dunaliella genus is known for the production of high added value molecules. In this study, strain AL-1 was isolated from the Sebkha of Sidi El Hani (Sousse, Tunisia). This isolate was identified both morphologically and genetically via 18S rRNA gene sequence as a member of the genus Dunaliella. Strain AL-1 was found to be closely related to Dunaliella salina, Dunaliella quartolecta and Dunaliella polymorpha with more than 97% similarity. Response surface methodology was used to maximize carotenoid production by strain AL-1 by optimizing its growth conditions. The highest carotenoid content was obtained at salinity: 51, light intensity: 189.89 μmol photons·m−2·s−1, and nitrogen: 60 mg·L−1. Proteomic profiling, using two-dimensional gel... |
| Tipo: Text |
Palavras-chave: Proteome; Dunaliella; 18S rRNA gene; Carotenoids; Bidimensional gel electrophoresis; Mass spectrometry. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00402/51398/51973.pdf |
| |
| Registros recuperados: 47 | |
|
|
|
