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| Registros recuperados: 42 | |
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| Gomes,Cristiano C.; Moreira,Leonardo M.; Santos,Vanessa J.S.V.; Ramos,Alfeu S.; Lyon,Juliana P.; Soares,Cristina P.; Santos,Fabio V.. |
| The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern. In this work, we used the MTT assay and micronucleus assay to examine the cytotoxicity and genotoxicity, respectively, of an extract obtained from this alloy. The MTT assay indicated that the mitochondrial activity and cell viability of CHO-K1 cells were unaffected by exposure to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biomaterial; Cytotoxicity; Micronucleus; Mutagenicity; Ti-6Al-4V. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100020 |
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| Grisolia,Cesar K.; Rivero,Carla L. G.; Starling,Fernando L. R. M.; Silva,Izabel C. R. da; Barbosa,Antonio C.; Dorea,Jose G.. |
| Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis , Cichla temensis , Hoplias malabaricus , Astyanax bimaculatus lacustres , Oreochromis niloticus , Cyprinus carpio and Steindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in Cichla temensis and Hoplias... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Fish biomonitoring; Eutrophic lake; Micronucleus; Comet assay; Cytotoxicity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100021 |
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| Roll,Mariana Matos; Miranda-Vilela,Ana Luisa; Longo,João Paulo Figueiró; Agostini-Costa,Tania da Silveira; Grisolia,Cesar Koppe. |
| Abstract Continued exposure to reactive oxygen species and inflammation are the rationale behind aging theories and associated diseases. Scientific evidence corroborates the ethnomedicinal use of the oil of pequi (Caryocar brasiliense Camb.), a typical Brazilian Cerrado fruit, against oxidative damage to biomolecules and inflammation. We aimed to investigate in vivo the antioxidant and anti-inflammatory effects of pequi oil on hemogram and DNA damage in healthy young adult and older middle-aged Swiss mice of both genders. Animals, aged 6-7 and 11-12 months, were orally treated for 15 days with pequi oil at 30 mg/day. Blood samples were used for hemogram and comet assay, and bone marrow for micronucleus test. Female controls of 11-12 months had... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aging-related inflammation and oxidative stress; Comet assay; Micronucleus; Antioxidant; Folk medicine. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500858 |
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| Matsumoto,Silvia Tamie; Mantovani,Mário Sérgio; Malaguttii,Mirtis Irene Ariza; Dias,Ana Lúcia; Fonseca,Inês Cristina; Marin-Morales,Maria Aparecida. |
| Cytotoxicity of metals is important because some metals are potential mutagens able to induce tumors in humans and experimental animals. Chromium can damage DNA in several ways, including DNA double strand breaks (DSBs) which generate chromosomal aberrations, micronucleus formation, sister chromatid exchange, formation of DNA adducts and alterations in DNA replication and transcription. In our study, water samples from three sites in the Córrego dos Bagres stream in the Franca municipality of the Brazilian state of São Paulo were subjected to the comet assay and micronucleus test using erythrocytes from the fish Oreochromis niloticus. Nuclear abnormalities of the erythrocytes included blebbed, notched and lobed nuclei, probably due to genotoxic chromium... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa; Chromium; Chromosomes aberrations; Comet assay; Micronucleus; Oreochromis niloticus. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100028 |
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| Ananthi,Rajagopal; Chandra,Nallathambi; Santhiya,Sathiyavedu T.. |
| The aqueous extract of Hemidesmus indicus roots was investigated for its in vivo antigenotoxic effect against cisplatin-induced cytogenetic damage. Swiss albino mice were administered with various doses of the extract either singly (50, 100 and 200 mg/kg body weight) or as split doses (10, 20 and 40 mg/kg bw/day) for five consecutive days by oral gavage. As endpoints, chromosome aberrations, micronuclei in polychromatic erythrocytes, mitotic index and PCE/NCE ratio were estimated. The extract protected the bone marrow cells from cisplatin-induced genotoxicity in an inverse dose-dependent manner. However, the extract was cytotoxic at all doses. But, under split dose regime it conferred a higher level of genoprotection and was not cytotoxic at the lower two... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genoprotective; Cytotoxic; Chromosomal aberrations; Micronucleus; Mitotic index. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100030 |
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| Bueno,Caroline Tanski; Silva,Cláudia Maria Dornelles da; Barcellos,Regina Bones; Silva,Juliana da; Santos,Carla Rossana dos; Menezes,João Evangelista Sampaio; Menezes,Honório Sampaio; Rossetti,Maria Lucia Rosa. |
| The aim of this study was to evaluate the association between the frequency of micronuclei (MN) and the cellular changes detected in the conventional Papanicolaou test. One hundred and seventy-four Papanicolaou test smears with cellular changes were examined. MN screening was done in cytopathological smears by counting 1,000 cervical cells in a light microscope. MN frequencies were significantly higher in the group with cellular changes compared to the control group (p < 0.001). The mean MN frequencies were 0.95 ± 1.12 (mean ± SD) in the control group (n = 223), 2.98 ± 1.20 in individuals with atypical squamous cells of undetermined significance (ASC-US) (n = 50), 4.04 ± 1.45 in cervical intraepithelial neoplasia (CIN) I (n = 52), 5.97 ± 1.83 in CIN II... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cervical cancer; Cervical lesions; Micronucleus; Papanicolaou test. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400004 |
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| Khouri,Juliana; Resck,Inês S.; Poças-Fonseca,Marcio; Sousa,Thiago M.M.; Pereira,Luciana O.; Oliveira,Adriano B.B.; Grisolia,Cesar K.. |
| The effectiveness of an aqueous extract of Caryocar brasiliense (Caryocaraceae) Camb pulp, popularly known in Brazil as pequi, against clastogenicity induced by cyclophosphamide and bleomycin was evaluated using an in vivo mouse bone marrow cell micronuclei test, an in vitro Chinese hamster ovary cell (CHO-K1) chromosome aberration test and an in vitro antioxidant assay based on the oxidative damage to 2-deoxy-D-ribose (2-DR) induced by hydroxyl radicals (OH) generated by the reaction between ascorbic acid and (Fe III)-EDTA. In mouse bone marrow cells the extract showed a protective effect against micronuclei induced by cyclophosphamide and bleomycin but did not interfere with polychromatic bone marrow erythrocyte proliferation, except when the mice had... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caryocar brasiliense; Anticlastogenicity; Antioxidant; Micronucleus. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300024 |
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| Santos,Fabio V.; Mesquita,Suzana F.P.; Faria,Maria José S.S.; Poersh,Aline; Maciel,Maria Aparecida M.; Pinto,Angelo C.; Morimoto,Helena K.; Cólus,Ilce Mara S.. |
| The plant Croton cajucara Benth. (Euphorbiaceae) is a medicinal plant from the Brazilian Amazon where it is commonly known as sacaca. The principal compound isolated from C. cajucara stem-bark extracts is the clerodane-type diterpene trans-dehydrocrotonin (DCTN) which presents several biological activities, including antiulcerogenic, anti-inflammatory, hypoglycemic, antimutagenic and antitumoral activity. However, few studies have been carried out to evaluate the therapeutic potential of raw C. cajucara extracts. We studied mutagenicity and antimutagenicity effects of C. cajucara methanol extract using the micronucleus assay in bone marrow cells and the dominant lethal assay in mice submitted to subchronic treatments. The blood testosterone levels of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimutagenicity; Mutagenicity; Dominant lethal; Micronucleus; Croton cajucara; Medicinal plant. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100029 |
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| Rodrigues,Fernando Postalli; Angeli,José Pedro Friedmann; Mantovani,Mário Sérgio; Guedes,Carmen Luisa Barbosa; Jordão,Berenice Quinzani. |
| Polycyclic aromatic hydrocarbons (PAHs) are genotoxic chemicals commonly found in effluents from oil refineries. Bioassays using plants and cells cultures can be employed for assessing environmental safety and potential genotoxicity. In this study, the genotoxic potential of an oil refinery effluent was analyzed by means of micronucleus (MN) testing of Alium cepa, which revealed no effect after 24 h of treatment. On the other hand, primary lesions in the DNA of rat (Rattus norvegicus) hepatoma cells (HTC) were observed through comet assaying after only 2 h of exposure. On considering the capacity to detect DNA damage of a different nature and of these cells to metabolize xenobiotics, we suggest the association of the two bioassays with these cell types,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allium cepa; HTC cells; Micronucleus; Comet assay; Petroleum waste disposal. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100028 |
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| Schneider,Beatriz Ursinos Catelan; Meza,Alisson; Beatriz,Adilson; Pesarini,João Renato; Carvalho,Pamela Castilho de; Mauro,Mariana de Oliveira; Karaziack,Caroline Bilhar; Cunha-Laura,Andréa Luiza; Monreal,Antônio Carlos Duenhas; Matuo,Renata; Lima,Dênis Pires de; Oliveira,Rodrigo Juliano. |
| Abstract Cardanol is an effective antioxidant and is a compound with antimutagenic and antitumoral activity. Here, we evaluated the genotoxic and mutagenic potential of saturated side chain cardanol and its effects in combination with cyclophosphamide in preventing DNA damage, apoptosis, and immunomodulation. Swiss mice were treated with cardanol (2.5, 5 and 10 mg/kg) alone or in combination with cyclophosphamide (100 mg/kg). The results showed that cardanol is an effective chemopreventive compound, with damage reduction percentages that ranged from 18.9 to 31.76% in the comet assay and from 45 to 97% in the micronucleus assay. Moreover, cardanol has the ability to reduce the frequency of apoptosis induced by cyclophosphamide. The compound did not show... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phenolic lipid; Antimutagenesis; Micronucleus; Comet assay; Apoptosis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200279 |
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| Miranda-Vilela,Ana L.; Resck,Inês S.; Grisolia,Cesar K.. |
| The daily consumption of natural antioxidants protects against oxidative damage caused by reactive oxygen species (ROS), including DNA damage, and can reduce the risk of cancer, atherosclerosis and other degenerative diseases. The pulp of pequi (Caryocar brasiliense Camb.) fruit, a tree native to the Brazilian savannah, contains several compounds with antioxidant properties, including carotenoids, vitamin C, phenolic compounds such as flavonoids, saponins and tannins, and essential oils. In this work, we examined the ability of organic and aqueous extracts of pequi fruit pulp to protect against the genotoxicity induced by two antineoplastic drugs, cyclophosphamide (CP) and bleomycin (BLM). Micronucleus tests with mouse bone marrow cells and single-cell gel... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antigenotoxic; Antioxidant; Caryocar brasiliense; Comet assay; Micronucleus; Pequi pulp extracts; Reactive oxygen species; TBARS assay. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500025 |
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| Thewes,Márcia Regina; Endres Junior,Delio; Droste,Annette. |
| The genotoxicity of untreated and treated sewage from two municipal wastewater treatment plants (WTP BN and WTP SJN) in the municipality of Porto Alegre, in the southern Brazilian state of Rio Grande do Sul, was evaluated over a one-year period using the Tradescantia pallida var. purpurea (Trad-MCN) bioassay. Inflorescences of T. pallida var. purpurea were exposed to sewage samples in February (summer), April (autumn), July (winter) and October (spring) 2009, and the micronuclei (MCN) frequencies were estimated in each period. The high genotoxicity of untreated sewage from WTP BN in February and April was not observed in treated sewage, indicating the efficiency of treatment at this WTP. However, untreated and treated sewage samples from WTP SJN had high... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Effluent; Micronucleus; Mutagenicity; Pollution; Trad-MCN. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400023 |
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| Cassel,Ana Paula Rebelo; Barcellos,Regina Bones; Silva,Cláudia Maria Dornelles da; Almeida,Sabrina Esteves de Matos; Rossetti,Maria Lucia Rosa. |
| The aim of the present study was to investigate the association between HPV-DNA and micronucleus (MN) frequency in women with normal cervical cytology. A total of 158 normal cervical smears were analyzed cytologically. The HPV genome was amplified using the GP5+/bioGP6+ consensus primers. HPV-DNA of high-risk types 16, 18, 31, 33, 39, 45 and 59 were also investigated. Of the 158 samples, 20 (12.7%) and 47 (29.7%) were positive for HPV-DNA and MN, respectively. Evidence for MN was found in 11 out of 20 (55%) HPV-DNA positive samples and in 36 out of 138 (26.1%) HPV-DNA negative ones. MN presence was significantly higher in HPV-DNA positive samples (p = 0.016). On the other hand, the absence of MN observed in a considerable number of HPV-DNA negative samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human papillomavirus; Micronucleus; Cytology; High-risk HPV; Southern Brazil. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300007 |
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| Bakare,Adekunle A.; Okunola,Alabi A.; Adetunji,Olusanmi A.; Jenmi,Hafeez B.. |
| Pharmaceutical industries are among the major contributors to industrial waste. Their effluents when wrongly handled and disposed of endanger both human and environmental health. In this study, we investigated the potential genotoxicity of a pharmaceutical effluent, by using the Allium cepa, mouse- sperm morphology, bone marrow chromosome aberration (CA) and micronucleus (MN) assays. Some of the physico-chemical properties of the effluent were also determined. The A. cepa and the animal assays were respectively carried out at concentrations of 0.5, 1, 2.5, 5 and 10%; and 1, 5, 10, 25 and 50% of the effluent. There was a statistically different (p < 0.05), concentration-dependent inhibition of onion root growth and mitotic index, and induction of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genotoxicity; Pharmaceutical effluent; Mouse; Allium cepa; Chromosome; Spermatozoa; Micronucleus. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200026 |
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| Registros recuperados: 42 | |
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