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Registros recuperados: 31
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ABC transporters in Mycoplasma hyopneumoniae and Mycoplasma synoviae: insights into evolution and pathogenicity Genet. Mol. Biol.
Nicolás,Marisa Fabiana; Barcellos,Fernando Gomes; Nehab Hess,Pablo; Hungria,Mariangela.
ABC transporters represent one of the largest superfamilies of active membrane transport proteins (MTPs) with a highly conserved ATPase domain that binds and hydrolyzes ATP, supplying energy for the uptake of a variety of nutrients and for the extrusion of drugs and metabolic wastes. The complete genomes of a non-pathogenic (J) and pathogenic (7448) strain of Mycoplasma hyopneumoniae, as well as of a pathogenic (53) strain of Mycoplasma synoviae have been recently sequenced. A detailed study revealed a high percentage of CDSs encoding MTPs in M. hyopneumoniae strains J (13.4%), 7448 (13.8%), and in M. synoviae 53 (11.2%), and the ABC systems represented from 85.0 to 88.6% of those CDSs. Uptake systems are mainly involved in cell nutrition and some might be...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ABC transporters; Bioinformatics; Genomes of microorganisms; Membrane transport proteins; Mycoplasma; Phylogeny of prokaryotes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200006
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Insertion sequences as variability generators in the Mycoplasma hyopneumoniae and M. synoviae genomes Genet. Mol. Biol.
Loreto,Elgion Lúcio Silva; Ortiz,Mauro Freitas; Porto,Jorge Ivan Rebelo.
We have analyzed the sequenced genomes of three strains of Mycoplasma hyopneumoniae and one strain of M. synoviae, and have found three and two different transposable element families, respectively in each species. In M. hyopneumoniae, the Insertion Sequences of the IS4 family is represented by ISMHp1, a putatively active element. The IS3 family is represented by several degenerated sequences. A third element called tMH was found, which shows some characteristics reminiscent of retrotransposons. In M. synoviae, three different possibly active IS4 elements are present (ISMHp1-like; ISMs1 and IS1634-like elements). The IS30 family is represented by the degenerated IS1630-like element. The IS1634-like element is shown to be involved in chromosomal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Insertion sequences; IS; Mollicutes; Horizontal gene transfer; Mycoplasma.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200017
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Kinases of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae: an overview Genet. Mol. Biol.
Bailão,Alexandre Melo; Parente,Juliana Alves; Pereira,Maristela; Soares,Célia Maria de Almeida.
Mycoplasma synoviae and Mycoplasma hyopneumoniae are wall-less eubacteria belonging to the class of Mollicutes. These prokaryotes have a reduced genome size and reduced biosynthetic machinery. They cause great losses in animal production. M. synoviae is responsible for an upper respiratory tract disease of chickens and turkeys. M. hyopneumoniae is the causative agent of enzootic pneumonia in pigs. The complete genomes of these organisms showed 17 ORFs encoding kinases in M. synoviae and 15 in each of the M. hyopneumoniae strain. Four kinase genes were restricted to the avian pathogen while three were specific to the pig pathogen when compared to each other. All deduced kinases found in the non pathogenic strain (J[ATCC25934]) were also found in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; Kinases; Genomes.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200008
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Comparative genome analysis of proteases, oligopeptide uptake and secretion systems in Mycoplasma spp Genet. Mol. Biol.
Staats,Charley Christian; Boldo,Juliano; Broetto,Leonardo; Vainstein,Marilene; Schrank,Augusto.
Mycoplasmas are very fastidious in their nutritional requirements for in vitro growth and have limited biosynthetic capacity, a reflection of their reduced genomes. As a result, these bacteria depend upon external metabolites for nutrition and growth and have developed dependence on their hosts for survival and maintenance. Protein degradation and peptide importation play an important role in Mycoplasma spp. nutrition, and proteases can play a role in host adaptation and pathogenicity. Here, we present a general survey on the genes involved in protein degradation, secretion and importation, comparing all available Mollicute genomes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; Proteases; Minimal genomes; Secretion systems.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200009
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Molecular analysis of an integrative conjugative element, ICEH, present in the chromosome of different strains of Mycoplasma hyopneumoniae Genet. Mol. Biol.
Pinto,Paulo Marcos; Carvalho,Marcos Oliveira de; Alves-Junior,Leonardo; Brocchi,Marcelo; Schrank,Irene Silveira.
Diversification of bacterial species and pathotypes is largely caused by lateral gene transfer (LGT) of diverse mobile DNA elements such as plasmids, phages, transposons and genomic islands. Thus, acquisition of new phenotypes by LGT is very important for bacterial evolution and relationship with hosts. This paper reports a 23 kb region containing fourteen CDSs with similarity to the previous described Integrative Conjugal Element of Mycoplasma fermentans (ICEF). This element, named ICEH, is present as one copy at distinct integration sites in the chromosome of 7448 and 232 pathogenic strains and is absent in the type strain J (non-pathogenic). Notable differences in the nucleotide composition of the insertion sites were detected, and could be correlated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: LGT; Mycoplasma; Constins.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200014
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A phylogenomic appraisal of the evolutionary relationship of mycoplasmas Genet. Mol. Biol.
Yotoko,Karla S.C.; Bonatto,Sandro L..
Several genomes of mycoplasmas have been sequenced and here we tried to retrieve the evolutionary relationships of nine species using a phylogenomic approach. Several methods were used to build phylogenetic trees based on protein sequence information, gene-order, and gene-content. We also utilized datasets composed of individual and concatenated sets of orthologous proteins, as well as with reduced unreliable alignment regions. Most of our results converge to a single topology, except for the trees built with both the maximum parsimony method and with the gene-order dataset. The gene-content dataset presented trees consistent with most nodes of the convergent tree, but in the gene-order dataset most internal branches were clearly saturated and unreliable....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phylogenomic; Mycoplasma; Phylogenetic methods; Maximum likelihood; Maximum parsimony.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200015
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Identification of the GTPase superfamily in Mycoplasma synoviae and Mycoplasma hyopneumoniae Genet. Mol. Biol.
Borges,Clayton Luiz; Parente,Juliana Alves; Pereira,Maristela; Soares,Célia Maria de Almeida.
Mycoplasmas are the smallest known prokaryotes with self-replication ability. They are obligate parasites, taking up many molecules of their hosts and acting as pathogens in men, animals, birds and plants. Mycoplasma hyopneumoniae is the infective agent of swine mycoplasmosis and Mycoplasma synoviae is responsible for subclinical upper respiratory infections that may result in airsacculitis and synovitis in chickens and turkeys. These highly infectious organisms present a worldwide distribution and are responsible for major economic problems. Proteins of the GTPase superfamily occur in all domains of life, regulating functions such as protein synthesis, cell cycle and differentiation. Despite their functional diversity, all GTPases are believed to have...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; GTPase superfamily; Genome.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200007
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Differential metabolism of Mycoplasma species as revealed by their genomes Genet. Mol. Biol.
Arraes,Fabricio B.M.; Carvalho,Maria José A. de; Maranhão,Andrea Q.; Brígido,Marcelo M.; Pedrosa,Fábio O.; Felipe,Maria Sueli S..
The annotation and comparative analyses of the genomes of Mycoplasma synoviae and Mycoplasma hyopneumonie, as well as of other Mollicutes (a group of bacteria devoid of a rigid cell wall), has set the grounds for a global understanding of their metabolism and infection mechanisms. According to the annotation data, M. synoviae and M. hyopneumoniae are able to perform glycolytic metabolism, but do not possess the enzymatic machinery for citrate and glyoxylate cycles, gluconeogenesis and the pentose phosphate pathway. Both can synthesize ATP by lactic fermentation, but only M. synoviae can convert acetaldehyde to acetate. Also, our genome analysis revealed that M. synoviae and M. hyopneumoniae are not expected to synthesize polysaccharides, but they can take...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mollicutes; Mycoplasma; Metabolism; Comparative genomics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200004
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Cosmopolitan Distribution of Endozoicomonas-Like Organisms and Other Intracellular Microcolonies of Bacteria Causing Infection in Marine Mollusks ArchiMer
Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W..
Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed....
Tipo: Text Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf
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Disturbance induced decoupling between host genetics and composition of the associated microbiome ArchiMer
Wegner, Karl Mathias; Volkenborn, Nils; Peter, Hannes; Eiler, Alexander.
Background Studies of oyster microbiomes have revealed that a limited number of microbes, including pathogens, can dominate microbial communities in host tissues such as gills and gut. Much of the bacterial diversity however remains underexplored and unexplained, although environmental conditions and host genetics have been implicated. We used 454 next generation 16S rRNA amplicon sequencing of individually tagged PCR reactions to explore the diversity of bacterial communities in gill tissue of the invasive Pacific oyster Crassostrea gigas stemming from genetically differentiated beds under ambient outdoor conditions and after a multifaceted disturbance treatment imposing stress on the host. Results While the gill associated microbial communities in...
Tipo: Text Palavras-chave: Microbiota; Population structure; Stress; Pathogen; Biological invasion; Pacific oyster; Crassostrea gigas; Vibrio; Mycoplasma.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00171/28202/26430.pdf
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Handbook of pigeonpea diseases Open Agri
M.V..
Palavras-chave: Pigeonpeas; Fungi; Phoma; Mycoplasma; Soil water regimes; Ratoons; Diseases; Sole cropping; Seed dressing; Soil solarization.
Ano: 1993 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4234
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Field diagnosis of groundnut diseases Open Agri
P..
Palavras-chave: Fungi; Groundnuts; Necrosis; Fruits; Diseases; Mycoplasma; Viruses; Bacteria; Environmental factors.
Ano: 1992 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4249
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Field diagnosis of chickpea diseases and their control Open Agri
Y.L..
Palavras-chave: Chickpeas; Fungi; Phoma; Vegetative stage; Mycoplasma; Necrosis; Diseases; Sowing date; Soil water content; Fruits.
Ano: 1991 URL: http://agropedia.iitk.ac.in/openaccess/?q=node/4248
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Economic Consequences of Biological Variation: The Case of Mycoplasma in Swine Herds AgEcon
Otto, Lars.
We present an economic decision support model, based on a Bayesian network, for Mycoplasma infection in slaughter swine production. The model describes the various risk factors for Mycoplasma infection and their interactions. This leads to a stochastic determination of the consequences of productivity factors, and this again results in stochastic economic consequences when changing the risk factors or engaging in control arrangements. We use the model to calculate how estimated prevalence and level of Mycoplasma changes when we gather evidence from various veterinarian examinations, and we show how this influences the distribution of the economic results of a change in strategy to fight Mycoplasma. One result is that the profitability in fighting...
Tipo: Conference Paper or Presentation Palavras-chave: Decision support system; Bayesian network; Pig; Mycoplasma; Livestock Production/Industries; C11; D81; Q12.
Ano: 2005 URL: http://purl.umn.edu/24458
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Circulation of Major Respiratory Pathogens in Backyard Poultry and their Association with Clinical Disease and Biosecurity Rev. Bras. Ciênc. Avic.
Batista,IA; Hoepers,PG; Silva,MFB; Nunes,PLF; Diniz,DCA; Freitas,AG; Cossi,MVC; Fonseca,BB.
SUMMARY Raising backyard birds is a common practice in Brazil, mainly in the countryside or suburban areas. However, the level of respiratory pathogens in these animals is unknown. We sampled two hundred chickens from 19 backyard flocks near commercial poultry farms and performed ELISA to Infectious Bronchitis Virus, avian Metapneumovirus, Mycoplasma synoviae and Mycoplasma gallisepticum. We evaluated the association between the predictive ability of ELISA and Hemagglutination-inhibition (HI)by comparing results from eight flocks positive to Mycoplasma gallisepticum on ELISA. Besides, we assessed essential biosecurity measures in the properties (multiple species birds, rodent control, hygienic conditions, and water quality for the bird`s consumption). We...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Avian Metapneumovirus; ELISA; Hemagglutination-inhibition; Infectious Bronchitis Virus; Mycoplasma.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000100324
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Serologic response of SPF chickens to live vaccines and other strains of Mycoplasma gallisepticum Rev. Bras. Ciênc. Avic.
Nascimento,ER do; Polo,P de A; Pereira,VL de A; Barreto,ML; Nascimento,M da GF do; Zuanaze,MAF; Corrêa,ARA; Silva,R de CF.
False positive serologic reactions and difficulties in the diagnosis of Mycoplasma gallisepticum (MG) in chickens have increased lately as a result of infection by low virulent MG strains and the use of live MG vaccines in poultry. The objective of this study was to evaluate the serologic responses of SPF chickens exposed to the three commercially available live MG vaccines, and one low virulent MG strain (MG-70), contributing to the diagnosis and monitoring of MG infection in birds. Six groups of SPF chickens were used. The control group was not infected nor challenged; one group was infected with the low virulent strain MG-70 (MG-70); three groups were immunized and named after the MG vaccine used, i.e., MG-6/85, MG-ts11, and MG-F; and finally one group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; Poultry; Serology; Vaccination.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100007
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Avian mycoplasmosis update Rev. Bras. Ciênc. Avic.
Nascimento,ER; Pereira,VLA; Nascimento,MGF; Barreto,ML.
Avian mycoplasmas occur in a variety of bird species. The most important mycoplasmas for chickens and turkeys are Mycoplasma gallisepticum (MG), M. synoviae (MS), and M. meleagridis. Besides, M. iowe (MI) is an emerging pathogen in turkeys, but of little concern for chickens. Mycoplasmas are bacteria that lack cell wall and belong to the class Mollicutes. Although they have been considered extracellular agents, scientists admit nowadays that some of them are obligatory intracellular microorganisms, whereas all other mycoplasmas are considered facultative intracellular organisms. Their pathogenic mechanism for disease include adherence to host target cells, mediation of apoptosis, innocent bystander damage to host cell due to intimate membrane contact,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Airsacculitis; Diagnosis; Mycoplasma; Mollicutes; Poultry.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2005000100001
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Hemoparasites and hemotropic bacteria detected through direct microscopy in dogs peripheric blood samples in a veterinarian private clinic in the municipality of Lorena, São Paulo, Brazil MV&Z
Gavião Prado, Luan; Vieira Sene, Rafael; Camargo de Medeiros, Elisângela; Maioli do Nascimento, Letícia; Beatriz França de Faria, Ana.
Hemoparasitosis, diseases that may cause clinical and hematological complications, are considered a great challenge to practitioners and clinical pathologists. Its high incidence in all Brazilian territory and the difficulty for a precise diagnosis can lead in most cases to a therapeutic diagnosis. Anaplasma platys, Babesia canis vogeli, Ehrlichia canis, Mycoplasma haemocanis and with a lower frequency and clinical relevance, Anaplasma phagocytophilum are the main etiological agents affecting dogs in Brazil. The climatic conditions found in several Brazilian areas, such as high rain and temperature levels, favor the arthropods vectors life cycles. This subject became an important challenge to both veterinarians and pet owners. These etiological agents...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hemoparasite; Anaplasma; Babesia; Mycoplasma; Ehrlichia; Hemoparasitose; Anaplasma; Babesia; Mycoplasma; Ehrlichia.
Ano: 2019 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/37999
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Plasmids in Mycoplasma species isolated from goats and sheep and their preliminary typing Rev. Microbiol.
Nascimento,Elmiro R.; DaMassa,Al J.; Yamamoto,Richard; Nascimento,M. Graça F..
One-hundred-five (105) clinical isolates of mycoplasma from caprine origin and one isolate from ovine were surveyed for plasmids, which were present in thirty-three (31%) of them. These mycoplasmas originated from 13 herds. Ten of them were symptomatic for mycoplasmal disease (mastitis, polyarthritis, septicemia) and three herds were asymptomatic, i.e., clinically normal. Twenty-eight isolates were Mycoplasma mycoides subspecies mycoides LC (large colony or caprine biotype), four were Mycoplasma capricolum subsp. capricolum and one was Mycoplasma cottewii. The isolated plasmids were linearized by EcoRI, EcoRV, EcoRI and EcoRV or BamHI and EcoRV, and were of five sizes (1.1, 1.6, 1.7, 1.8, and 1.9 Kbp). Based on restriction enzyme digestion and size of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; Plasmid; Goat; Endonuclease; Electrophoresis.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100006
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Molecular biology of mycoplasmas: from the minimum cell concept to the artificial cell Anais da ABC (AABC)
CORDOVA,CAIO M.M.; HOELTGEBAUM,DANIELA L.; MACHADO,LAÍS D.P.N.; SANTOS,LARISSA DOS.
ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycoplasma; Molecular biology; Microbial genetics; Synthetic biology; Gene transfer techniques.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599
Registros recuperados: 31
Primeira ... 12 ... Última
 

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