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Registros recuperados: 46 | |
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Castro-Silva,Carolina; Ruíz-Valdiviezo,Víctor M; Valenzuela-Encinas,César; Alcántara-Hernández,Rocio J; Navarro-Noya,Yendi E; Vázquez-Núñez,Edgar; Luna-Guido,Marco; Marsch,Rodolfo; Dendooven,Luc. |
Background: The application of polycyclic aromatic hydrocarbons (PAHs) will affect the bacterial community structure as some groups will be favoured and others not. An alkaline saline soil with electrolytic conductivity (EC) 56 dS m-1 was spiked with anthracene and acetone while their effect on bacterial community structure was investigated. Results: The percentages of Acidobacteria and Actinobacteria decreased over time, while the percentage of Proteobacteria, mostly Xanthomonadales, increased. The percentage of the phylotypes belonging to the Nocardioides, Rhodococcus and Streptomyces, known degraders of PAHs, was larger in the anthracene-amended soil than in the acetone-amended and unamended soil at day 14. The phylotypes belonging to the genera... |
Tipo: Journal article |
Palavras-chave: C dynamics; N mineralization; Phylogenetic analysis; Polycyclic aromatic hydrocarbons; Principal component analysis; UniFrac. |
Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500010 |
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Ke,Fei; Wang,Yun; Yang,Chuan-Shun; Xu,Chen. |
Background Hepcidins, a kind of cysteine-rich antimicrobial peptides, play important roles in host immunological processes and iron regulation, which have been identified from several fish species. The rare minnow (Gobiocypris rarus), an endemic cyprinid fish in China, has been used extensively as model animal in laboratory. However, little is known about its hepcidin. Here, we report the cloning and characterization of a hepcidin gene from the liver of Chinese rare minnow. Results The full-length cDNA of rare minnow hepcidin is 662 bp, which contains an ORF of 273 bp encoding a prepropeptide of 90 amino acid residues. The predicted prepropeptide contains three domains: a signal peptide of 24 amino acids, a prodomain of 41 amino acids, and a mature peptide... |
Tipo: Journal article |
Palavras-chave: Cysteine residues; Phylogenetic analysis; Rare minnow hepcidin; Synthetic peptide. |
Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300005 |
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Hu,Lifang; Yang,Yingui; Jiang,Lunwei; Liu,Shiqiang. |
Abstract Catalase (CAT) is a common antioxidant enzyme in almost all living organisms. Currently, detailed reports on cucumber (Cucumis sativus L.) CAT (CsCAT) genes and tissue expression profiling are limited. In the present study, four candidate CsCAT genes were identified in cucumber. Phylogenetic analysis indicated that CsCAT1-CsCAT3 are closely related to Arabidopsis AtCAT1-AtCAT3, but no obvious counterpart was observed for CsCAT4. Intron/exon structure analysis revealed that only one of the 15 positions was completely conserved. Motif analysis showed that, unlike the CAT genes of other species, none of CsCAT genes contained all 10 motifs. Expression data showed that transcripts of all of the CsCAT genes, except CsCAT4, were detected in five tissues.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cucumis sativus L.; Catalase; Phylogenetic analysis; Gene family; Motif. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300408 |
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Rabello,Aline R.; Queiroz,Paulo R.; Simões,Kenya C.C.; Hiragi,Cássia O.; Lima,Luzia H.C.; Oliveira,Maria Regina V.; Mehta,Angela. |
The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Whitefly; ITS rDNA; Genetic diversity; Phylogenetic analysis. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300029 |
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Li,Huixin; Liu,Shengwang; Han,Zongxi; Shao,Yuhao; Chen,Shuhong; Kong,Xiangang. |
The nucleotide sequences of eight open reading frames (ORFs) located at the 5' end of the unique long region of the duck enteritis virus (DEV) Clone-03 strain were determined. The genes identified were designated UL1 , UL2 , UL3 , UL4 , UL5 , UL6 and UL 7 homologues of the herpes simplex virus 1 (HSV-1). The DEV UL3.5 located between UL3 and UL4 had no homologue in the HSV-1. The arrangement and transcription orientation of the eight genes were collinear with their homologues in the HSV-1. Phylogenetic trees were constructed based on the alignments of the deduced amino acids of eight proteins with their homologues in 12 alpha-herpesviruses. In the UL1, UL3, UL3.5, UL5 and UL7 proteins trees, the branches were more closely related to the genus... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duck enteritis virus; UL1-UL7 genes; Phylogenetic analysis. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100018 |
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Inglis,Peter W.; Tigano,Myrian S.. |
A phylogenetic analysis of the 5.8S rDNA and internal transcribed spacer (ITS1 and ITS2) sequences from some entomogenous Paecilomyces species supports the polyphyly of the genus and showed the existence of cryptic species. In the Eurotiales, anamorphs Paecilomyces variotii and Paecilomyces leycettanus were related to the teleomorphs Talaromyces and Thermoascus. In the Hypocreales, three major ITS subgroups were found, one of which included Paecilomyces viridis, Paecilomyces penicillatus, Paecilomyces carneus and isolates identified as Paecilomyces lilacinus and Paecilomyces marquandii. However, the majority of the P. lilacinus and P. marquandii isolates formed a distinct and distantly related subgroup, while the other major subgroup contained Paecilomyces... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paecilomyces spp.; Phylogenetic analysis; RDNA; Molecular taxonomy. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100025 |
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Zhao,Shan; Jiang,Qian-Tao; Ma,Jian; Wang,Ji-Rui; Liu,Ya-Xi; Chen,Guo-Yue; Qi,Peng-Fei; Pu,Zhi-En; Lu,Zhen-Xiang; Zheng,You-Liang; Wei,Yu-Ming. |
The WUSCHEL (WUS)-related homeobox (WOX) gene family coordinates transcription during the early phases of embryogenesis. In this study, a putative WOX2 homolog was isolated and characterized from Aegilops tauschii, the donor of D genome of Triticum aestivum. The sequence consisted of 2045 bp, and contained an open reading frame (ORF), encoded 322 amino acids. The predicted protein sequence contained a highly conserved homeodomain and the WUS-box domain, which is present in some members of the WOX protein family. The full-length ORF was subcloned into prokaryotic expression vector pET-30a, and an approximately 34-kDa protein was expressed in Escherichia coli BL21 (DE3) cells with IPTG induction. The molecular mass of the expressed protein was identical to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: WOX2; Aegilops tauschii; Phylogenetic analysis; Real-time quantitative PCR. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100079 |
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Liang,Qinglong; Wei,Lei; Wang,Xinwei; He,Hongxuan. |
MHC class I proteins mediate functions in anti-pathogen defense. MHC diversity has already been investigated by many studies in model avian species, but here we chose the bar-headed goose, a worldwide migrant bird, as a non-model avian species. Sequences from exons encoding the peptide-binding region (PBR) of MHC class I molecules were isolated from liver genomic DNA, to investigate variation in these genes. These are the first MHC class I partial sequences of the bar-headed goose to be reported. A preliminary analysis suggests the presence of at least four MHC class I genes, which share great similarity with those of the goose and duck. A phylogenetic analysis of bar-headed goose, goose and duck MHC class I sequences using the NJ method supports the idea... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MHC I; Anser indicus; Polymorphism; Phylogenetic analysis. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300031 |
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Rocha,Graça Celeste Gomes; Corrêa,Régis Lopes; Borges,Anna Cristina Neves; Sá,Claudio Bustamante Pereira de; Alves-Ferreira,Márcio. |
Homeobox genes encode transcriptional factors, usually involved in molecular control of plant developmental patterns. They can be divided into several classes according to conserved sequences within the homeobox region and the presence of specific additional sequences. Based on these conserved sequences, we developed a search procedure to identify possible homeobox genes in the Eucalyptus Genome Sequencing Project Consortium (FORESTs) database. We were able to identify 50 Eucalyptus sequences (EST-contigs) containing the homeodomain sequence. Phylogenetic analysis was applied to this ESTs-contigs and 44 of them were found to have similarities with one of three well-known homeobox classes: Bell, Knox and HD-Zip, and their sub-classes. However, no EST-contig... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant development; Abiotic stress; Eucalyptus; FORESTs; Phylogenetic analysis. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400005 |
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Hu,Lifang; Liu,Shiqiang. |
Members of the ERF transcription-factor family participate in a number of biological processes, viz., responses to hormones, adaptation to biotic and abiotic stress, metabolism regulation, beneficial symbiotic interactions, cell differentiation and developmental processes. So far, no tissue-expression profile of any cucumber ERF protein has been reported in detail. Recent completion of the cucumber full-genome sequence has come to facilitate, not only genome-wide analysis of ERF family members in cucumbers themselves, but also a comparative analysis with those in Arabidopsis and rice. In this study, 103 hypothetical ERF family genes in the cucumber genome were identified, phylogenetic analysis indicating their classification into 10 groups, designated I to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cucumis sativus L.; ERF; Phylogenetic analysis; Transcription factor; Genome sequence. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400015 |
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Ramana Gopavajhula,V.; Viswanatha Chaitanya,K.; Akbar Ali Khan,P.; Shaik,Jilani P.; Narasimha Reddy,P.; Alanazi,Mohammad. |
Superoxide dismutase (SOD, EC 1.15.1.1) is an important metal-containing antioxidant enzyme that provides the first line of defense against toxic superoxide radicals by catalyzing their dismutation to oxygen and hydrogen peroxide. SOD is classified into four metalloprotein isoforms, namely, Cu/Zn SOD, Mn SOD, Ni SOD and Fe SOD. The structural models of soybean SOD isoforms have not yet been solved. In this study, we describe structural models for soybean Cu/Zn SOD, Mn SOD and Fe SOD and provide insights into the molecular function of this metal-binding enzyme in improving tolerance to oxidative stress in plants. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acid analysis; Model evaluation; Molecular modeling; Phylogenetic analysis; Superoxide dismutase (SOD). |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200013 |
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Ishida,Juliane Karine; Caldas,Danielle G.G.; Oliveira,Lucas Roberto; Frederici,Gabriela Campos; Leite,Lucas Margato Pereira; Mui,Tsai Siu. |
Abstract Transporter proteins play an essential role in the uptake, trafficking and storage of metals in plant tissues. The Natural Resistance-Associated Macrophage Protein (NRAMP) family plays an essential role in divalent metal transport. We conducted bioinformatics approaches to identify seven NRAMP genes in the Phaseolus vulgaris genome, investigated their phylogenetic relation, and performed transmembrane domain and gene/protein structure analyses. We found that the NRAMP gene family forms two distinct groups. One group included the PvNRAMP1, -6, and -7 genes that share a fragmented structure with a numerous exon/intron organization and encode proteins with mitochondrial or plastidial localization. The other group is characterized by few exons that... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Transporter; NRAMP; Gene family; Phylogenetic analysis. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000500820 |
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Kothe, Caroline; Delbarre-ladrat, Christine; Renault, Pierre; Passerini, Delphine. |
Halophilic and psychrophilic marine bacteria are source of interesting bioactive molecules for biotechnology. We report here the whole-genome sequences of two of them, Pseudoalteromonas sp. MIP2626 isolated from tropical peeled shrimps and Psychrobacter sp. BI730 isolated from deep-sea hydrothermal vent. Sequencing of both genomes was performed by Illumina HiSeq platform (2 × 150 pb). De novo assemblies using Spades v3.9 generated 136 contigs for Pseudoalteromonas MIP2626 and 42 contigs for Psychrobacter BI730, representing a genome size of 3.9 Mb and 3.2 Mb, respectively. Phylogenetic based on 16S rRNA gene sequence and phylogenomic analyses were reported to compare the new sequences with Pseudoalteromonas and Psychrobacter representative strains... |
Tipo: Text |
Palavras-chave: Aquatic; Genomic; Phylogenetic analysis; Halophilic. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00635/74662/74568.pdf |
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Parnell, J.. |
Four different analyses of Thai Syzygium show, with very small discrepancies, that one smaller monophyletic and one larger polyphyletic group can be recognised. The smaller monophyletic group warrants sectional rank as Syzygium section Jambosa and consists of S. anacardiifolium, S. aqueum, S. diospyrifolium, S. formosum, S. foxworthianum, S. jambos, S. lakshanakarae, S. malaccense, S. megacarpum, S. papillosum, S. pseudoformosum, S. pycnanthum, S. samarangense, S. scortechinii and S. siamense. |
Tipo: Article / Letter to the editor |
Palavras-chave: Eugenia; Jambosa; Syzygium; Cladistics; Phenetic analysis; Phylogenetic analysis. |
Ano: 1999 |
URL: http://www.repository.naturalis.nl/record/525752 |
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Jaklitsch, W.M.; Gardiennet, A.; Voglmayr, H.. |
Fresh material, type studies and molecular phylogeny were used to clarify phylogenetic relationships of the nine genera Acrocordiella, Blogiascospora, Clypeosphaeria, Hymenopleella, Lepteutypa, Pseudapiospora, Requienella, Seiridium and Strickeria. At first sight, some of these genera do not seem to have much in common, but all were found to belong to the Xylariales, based on their generic types. Thus, the most peculiar finding is the phylogenetic affinity of the genera Acrocordiella, Requienella and Strickeria, which had been classified in the Dothideomycetes or Eurotiomycetes, to the Xylariales. Acrocordiella and Requienella are closely related but distinct genera of the Requienellaceae. Although their ascospores are similar to those of Lepteutypa,... |
Tipo: Article / Letter to the editor |
Palavras-chave: Ascomycota; Dothideomycetes; New genus; Phylogenetic analysis; Pyrenomycetes; Pyrenulales; Sordariomycetes; Xylariales. |
Ano: 2016 |
URL: http://www.repository.naturalis.nl/record/611854 |
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Registros recuperados: 46 | |
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