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Registros recuperados: 23 | |
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Djelic,Ninoslav; Djelic,Dijana; Spremo-Potparevic,Biljana; Zivkovic,Lada; Markovic,Biljana; Lozance,Olivera; Blagojevic,Milos. |
Thyroid hormones stimulate aerobic metabolism which may lead to oxidative stress accompanied by damage to various cellular macromolecules, including DNA. Previous comet assay studies have shown that thyroid hormones cause DNA damage due to the creation of reactive oxygen species (ROS). However, cytogenetic studies have been equivocal because although an increase in the sister-chromatid exchange frequency per cell has been reported increased micronuclei frequency has not. We used cytogenetic examination of chromosome breakage and aberrations in whole-blood cultures of human peripheral blood lymphocytes to investigate possible clastogenic effects when lymphocytes were exposed to 0.002 µM to 50 µM of L-thyroxine for 24 h and 48 h, these concentrations being... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thyroxine; Human lymphocytes; Chromosome aberrations; Mitotic index; ROS. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600019 |
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Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Haure, Joel; Bedier, Edouard; Ropert, Michel; Moal, Jeanne; Huvet, Arnaud; Bacca, Helene; Van Wormhoudt, A; Delaporte, Maryse; Costil, K; Pouvreau, Stephane; Lambert, Christian; Boulo, Viviane; Soudant, Philippe; Nicolas, Jean-louis; Leroux, Frederique; Renault, Tristan; Gagnaire, Beatrice; Geret, Florence; Boutet, Isabelle; Burgeot, Thierry; Boudry, Pierre. |
Summer mortality of Pacific oysters is known in several countries. However no specific pathogen has been systematically associated with this phenomenon. A complex combination of environmental and biological parameters has been suggested as the cause and is now starting to be identified. A high genetic basis was found for survival in oysters when a first generation (G1) was tested in three sites during summer. This paper presents a synthesis on physiological characteristics of two selected groups ('R' and 'S', from families selected for resistance and susceptibility to summer mortality respectively), of the second and third generations. R and S showed improvement or reduction of survival compared with the control in both field and laboratory trials... |
Tipo: Text |
Palavras-chave: ROS; Temperature; Stress; Hemocytes; Reproduction; Genetic selection; Resistant oyster; Crassostrea gigas; Pacific Oyster; Summer mortality. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2809.pdf |
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Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris. |
Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas... |
Tipo: Text |
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf |
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Dupraz, Valentin; Ménard, Dominique; Akcha, Farida; Budzinski, Hélène; Stachowski-haberkorn, Sabine. |
This study screened binary mixtures of pesticides for potential synergistic interaction effects on growth of the marine microalgae Tisochrysis lutea and Skeletonema marinoi. It also examined the single and combined effects of three of the most toxic substances on microalgal physiology. Single substances were first tested on each microalgal species to determine their respective EC50 and concentration-response relationships. The toxicity of six and seven binary mixtures was then evaluated in microplate experiments on the growth of T. lutea and S. marinoi, respectively, using two mixture modelling approaches: isobolograms and the MIXTOX tool, based on Concentration Addition (CA) or Independent Action (IA) models. Significant cases of antagonism (for both... |
Tipo: Text |
Palavras-chave: ROS; Photosynthesis; Lipids; Synergy; Isoproturon; Metazachlor. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00488/59928/63131.pdf |
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AGOSTINETTO,D.; PERBONI,L.T.; LANGARO,A.C.; GOMES,J.; FRAGA,D.S.; FRANCO,J.J.. |
The use of herbicides, even in tolerant crops, can cause stress evidenced by increase phytotoxicity affecting growth and development. The objectives of this study were to evaluate herbicides effect from different mechanisms of action in photosynthetic and oxidative stress parameters, as well visual phytotoxicity and wild radish control in wheat crop, cultivar Quartzo. Two trials were conducted where the first one evaluated the photosynthetic parameters on wheat plants in two seasons collection, following the application of herbicides bentazon, clodinafop, iodosulfuron, metribuzin, metsulfuron and 2,4-D; and the second one evaluated wild radish (Raphanus sativus) control, wheat phytotoxicity and yield due to bentazon, iodosulfuron, metribuzin, metsulfuron... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum; Selectivity; ROS; Antioxidant enzymes. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000100001 |
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Almeida, Lorena Gabriela; Silva, Dayane Meireles da; Jorge, Adelcio de Paula; Souza, Kamila Rezende Dázio de; Guilherme, Luiz Roberto Guimarães; Alves, José Donizeti. |
Agricultural species are subjected to a variety of biotic and abiotic stresses, which are the main limitations to crop production. In this context, contamination by trace elements is characterized as an abiotic stress that represents an environmental problem. Due to the physical and chemical similarities between cadmium and zinc, these elements may interact in the environment and may cause antagonistic or synergistic effects. In this way, physiological mechanisms to exclude, detoxify or compartmentalize trace elements that are in excess are crucial for plant survival when exposed to high concentrations of these elements. In this way, the aim of this study was to understand the physiological responses of Phaseolus vulgaris plants subjected to increasing... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trace elements; Antioxidante enzymes; ROS; Photosynthesis; Synergism. Fisiologia Vegetal. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35829 |
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Almeida, Lorena Gabriela; Silva, Dayane Meireles da; Jorge, Adelcio de Paula; Souza, Kamila Rezende Dázio de; Guilherme, Luiz Roberto Guimarães; Alves, José Donizeti. |
Agricultural species are subjected to a variety of biotic and abiotic stresses, which are the main limitations to crop production. In this context, contamination by trace elements is characterized as an abiotic stress that represents an environmental problem. Due to the physical and chemical similarities between cadmium and zinc, these elements may interact in the environment and may cause antagonistic or synergistic effects. In this way, physiological mechanisms to exclude, detoxify or compartmentalize trace elements that are in excess are crucial for plant survival when exposed to high concentrations of these elements. In this way, the aim of this study was to understand the physiological responses of Phaseolus vulgaris plants subjected to increasing... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trace elements; Antioxidante enzymes; ROS; Photosynthesis; Synergism.; Fisiologia Vegetal. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35829 |
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KWON,DA HYE; JEONG,JIN WOO; CHOI,EUN OK; LEE,HYE WON; LEE,KI WON; KIM,KI YOUNG; KIM,SUNG GOO; HONG,SU HYUN; KIM,GI-YOUNG; PARK,CHEOL; HWANG,HYE-JIN; SON,CHANG-GUE; CHOI,YUNG HYUN. |
ABSTRACT Mori folium, the leaf of Morus alba L. (Moraceae), has been traditionally used for various medicinal purposes from ancient times to the present. In this study, we examined the effects of water extract of Mori folium (WEMF) on the production of inflammatory mediators, such as nitric oxide (NO) and prostaglandin E2 (PGE2), and reactive oxygen species (ROS) in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 macrophages. Our data indicated that WEMF significantly suppressed the secretion of NO and PGE2 in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects were accompanied by a marked reduction in their regulatory gene expression at the transcription level. WEMF attenuated LPS-induced intracellular ROS production in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mori folium; Inflammation; ROS; Macrophage; Zebrafish. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000200661 |
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Pieme,Constant Anatole; Santosh,Guru Kumar; Tekwu,Emmanuel Mouafo; Askun,Tülin; Aydeniz,Hatice; Ngogang,Jeanne Yonkeu; Bhushan,Shashi; Saxena,Ajit Kumar. |
BACKGROUND: Zanthoxylum heitzii is a spice used to prepare several dishes and to treat tumors, syphilis, malaria, cardiac palpitations, urogenital infections in the west region of Cameroon, but the antitumor mechanisms and chemical composition are not yet investigated. This study was aimed to determine the antiproliferative effects of four extracts from the fruits and barks of Zanthoxyllum heitzii (Rutaceae) on apoptosis in human promyelocytic cells, their mechanisms and the chemical composition. The 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the fifty percent inhibition (IC50) concentration of the cell lines after treatment. The effect on morphology was observed using a light or fluorescence... |
Tipo: Journal article |
Palavras-chave: Zanthozyllum heitzii; Syringic acid; Apoptosis; HL-60 cells; ROS; Mitochondrial membrane potential. |
Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100048 |
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Otunola,Gloria A; Oloyede,Oyelola B; Oladiji,Adenike T; Afolayan,Anthony J. |
BACKGROUND: Effect of aqueous extracts of Allium sativum (garlic), Zingiber officinale (ginger), Capsicum fructensces (cayenne pepper) and their mixture on oxidative stress in rats fed high Cholesterol/high fat diet was investigated. Rats were randomly distributed into six groups (n = 6) and given different dietary/spice treatments. Group 1 standard rat chow (control), group 2, hypercholesterolemic diet plus water, and groups 3, 4, 5, 6, hypercholesterolemic diet with 0.5 ml 200 mg · kg-1 aqueous extracts of garlic, ginger, cayenne pepper or their mixture respectively daily for 4 weeks. RESULTS: Pronounced oxidative stress in the hypercholesterolemic rats evidenced by significant (p < 0.05)... |
Tipo: Journal article |
Palavras-chave: Lipid peroxidation; Oxidative stress; Spices; Hypercholesterolemia; ROS. |
Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100005 |
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Mukherjee,Debarati; Singh,Chingakham Brajakishor; Dey,Somaditya; Mandal,Supratim; Ghosh,Joydip; Mallick,Suvadip; Hussain,Aabid; Swapana,Ningombam; Ross,Samir Anis; Pal,Chiranjib. |
Abstract In the present context of emergence of resistance aligned with the conventional anti-leishmanial drugs and occasional treatment failure compelled us to continue the search for replaceable therapeutic leads against Leishmaniainfection. Various ginger spices of the Zingiberaceae family are widely used as spices, flavouring agents, and medicines in Southeast Asia because of their unique flavour as well as due to their medicinal properties. Zerumbone, a natural component of Zingiber zerumbet (L.) Smith, has been studied for its pharmacological potential as antiulcer, antioxidant, anticancer, and antimicrobial. In this study, we have shown that zerumbone could induce ROS mediated apoptosis in Leishmania donovani promastigotes and also found effective... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania donovani; Zingiber zerumbet; Zerumbone; Anti-leishmanial; ROS. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000100048 |
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Sá,N.A.R.; Bruno,J.B.; Guerreiro,D.D.; Cadenas,J.; Alves,B.G.; Cibin,F.W.S.; Leal-Cardoso,J.H.; Gastal,E.L.; Figueiredo,J.R.. |
Primordial follicles, the main source of oocytes in the ovary, are essential for the maintenance of fertility throughout the reproductive lifespan. To the best of our knowledge, there are no reports describing the effect of anethole on this important ovarian follicle population. The aim of the study was to investigate the effect of different anethole concentrations on the in vitro culture of caprine preantral follicles enclosed in ovarian tissue. Randomized ovarian fragments were fixed immediately (non-cultured treatment) or distributed into five treatments: α-MEM+ (cultured control), α-MEM+ supplemented with ascorbic acid at 50 μg/mL (AA), and anethole at 30 (AN30), 300 (AN300), or 2000 µg/mL (AN2000), for 1 or 7 days. After 7 days of culture, a... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant; Anethole; ROS; Primordial follicle; Caprine. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800602 |
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Xie,X.X.; Ma,Y.F.; Wang,Q.S.; Chen,Z.L.; Liao,R.R.; Pan,Y.C.. |
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. Yeast CUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress. In this study, CUP1 expression was determined by quantitative real-time PCR, and copper content was detected by inductively coupled plasma mass spectrometry. Production of intracellular reactive oxygen species (ROS) was evaluated using the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assay. Cellular viability was detected using the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yeast; Overexpression; Copper stress; Viability; ROS. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700616 |
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Zhang,You En; Huang,Guang Qing; Wu,Bing; Lin,Xin Duo; Yang,Wen Zi; Ke,Zun Yu; Liu,Jie. |
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Reactive oxygen species; ROS; Hydrogen sulfide; H2O2-induced apoptosis; Cardiomyocytes. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000400608 |
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Maiti,Saborni; Ghosh,Nirmalia; Mandal,Chiranjib; Das,Kingsuk; Dey,Narottam; Adak,Malay Kumar. |
Two maize varieties, viz. Sartaj and Deccan, have been evaluated according to their responses to chromium (Cr) in terms of specific physiological and biochemical attributes. During the exposure to Cr concentration (0, 50, 100, 200 and 300 µM) of potassium dichromate (K2Cr2O7) for seven days, significant changes of oxidative stress in terms of lipid peroxidation and protein oxidation were recorded along with other antioxidative pathways. It is revealed that irrespective of varieties, Sartaj was more prone to oxidative stress since the amount of superoxide due to Cr exposure was found to be significant, unlike the second variety, Deccan. In addition, for Sartaj the activity of NAD(P)H oxidase recorded a significant linear increase in a dose response... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidative enzymes; MDA; Protein oxidation; ROS. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000300007 |
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Registros recuperados: 23 | |
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