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Abscisic acid and the antioxidant system are involved in germination of Butia capitata seeds Acta Botanica
Bicalho,Elisa Monteze; Santos,Talita R. Silva dos; Garcia,Queila Souza.
ABSTRACT Seed germination is an important step for plants without vegetative propagation and is a physiological process that begins with specific environmental cues resulting in biochemical responses. Breaking-dormancy is necessary to study germination in dormant seeds with asynchronous germination. We investigated the processes of breaking dormancy and germination of Butia capitata (Arecaceae) seeds, in which germination is slow and asynchronous, by operculum removal. This treatment increased germination of B. capitata to 90 %. Embryos of dry, imbibed, 24-hours post-operculum removal and early-germinated seeds were collected for biochemical analysis of the following: quantification of abscisic acid (ABA) and hydrogen peroxide (H2O2), activities of...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Breaking-dormancy; Haustorium; Hormones; Palm seeds; Operculum; Physiological dormancy; Reactive oxygen species.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000100174
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Antioxidative enzymes of Cucumis sativus seeds are modulated by Leucaena leucocephala extracts Biological Sciences
Ribeiro, Vandjore de Mattos; Spiassi, Ariane; Marcon, Thaís Regina; Lima, Gislaine Piccolo de; Corsato, Jaqueline Malagutti; Fortes, Andréa Maria Teixeira.
 This study aimed to analyze oxidative stress produced in cucumber seeds and seedlings when exposed to aqueous extract from dried leaves of leucaena, as well as its effect on the germination behavior, early growth and the antioxidant enzymes activity. It was evaluated the percentage, the speed index, the average time, the frequency and germination synchronization, the root length and shoot, as well as the catalase and peroxidase enzymes activity. There was no significant inhibitory effect of the leaf extracts on the germination percentage. However, there was delay in the seeds germination, as the extract proportion increased. A stimulatory effect of the extract compared to the shoot length was observed, however the root growth was significantly reduced....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Botânica allelopathy; Reactive oxygen species; Antioxidant enzymes. Fisiologia Vegetal.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34801
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Macrophages as target cells for Mayaro virus infection: involvement of reactive oxygen species in the inflammatory response during virus replication Anais da ABC (AABC)
CAVALHEIRO,MARIANA G.; COSTA,LEANDRO SILVA DA; CAMPOS,HOLMES S.; ALVES,LETÍCIA S.; ASSUNÇÃO-MIRANDA,IRANAIA; POIAN,ANDREA T. DA.
ABSTRACT Alphaviruses among the viruses that cause arthritis, consisting in a public health problem worldwide by causing localized outbreaks, as well as large epidemics in humans. Interestingly, while the Old World alphaviruses are arthritogenic, the New World alphaviruses cause encephalitis. One exception is Mayaro virus (MAYV), which circulates exclusively in South America but causes arthralgia and is phylogenetically related to the Old World alphaviruses. Although MAYV-induced arthritis in humans is well documented, the molecular and cellular factors that contribute to its pathogenesis are completely unknown. In this study, we demonstrated for the first time that macrophages, key players in arthritis development, are target cells for MAYV infection,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alphaviruses; Mayaro virus; Macrophage activation; Reactive oxygen species; Viral arthritis.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401485
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Oxidative stress and fish immune system: phagocytosis and leukocyte respiratory burst activity Anais da ABC (AABC)
BILLER,JAQUELINE D.; TAKAHASHI,LEONARDO S..
Abstract Molecular oxygen is a necessary compound for all aerobic organisms, although oxygen is a potent oxidant, which can cause oxidative stress (OS). OS occurs when there is an imbalance between the production of oxidant and antioxidants components, are result of normal cell metabolism, and many of these compounds play a fundamental role in several metabolic pathways. The organism produces several reactive oxygen species (ROS), but they are balanced by an antioxidant defense system that maintains the levels of these oxidizing compounds at an acceptable level. Many of these components are essential in the organism defense and their byproducts are considered potent bactericides that actively act in the destruction of invading pathogens. Fish immune system...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Innate immunity; Antioxidant system; Free radical.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000703403
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An overview of chagasic cardiomyopathy: pathogenic importance of oxidative stress Anais da ABC (AABC)
Zacks,Michele A.; Wen,Jian-Jun; Vyatkina,Galina; Bhatia,Vandanajay; Garg,Nisha.
There is growing evidence to suggest that chagasic myocardia are exposed to sustained oxidative stress-induced injuries that may contribute to disease progression. Pathogen invasion- and replication-mediated cellular injuries and immune-mediated cytotoxic reactions are the common source of reactive oxygen species (ROS) in infectious etiologies. However, our understanding of the source and role of oxidative stress in chagasic cardiomyopathy (CCM) remains incomplete. In this review, we discuss the evidence for increased oxidative stress in chagasic disease, with emphasis on mitochondrial abnormalities, electron transport chain dysfunction and its role in sustaining oxidative stress in myocardium. We discuss the literature reporting the consequences of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chagasic cardiomyopathy; Reactive oxygen species; Inflammation; Mitochondria; Oxidant/antioxidant status; Oxidative damage.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000400009
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Candesartan inhibits inflammation through an angiotensin II type 1 receptor independent way in human embryonic kidney epithelial cells Anais da ABC (AABC)
YU,YING; JIANG,HAIFENG; NIU,YANGYANG; ZHANG,XIAOQIN; ZHANG,YINGYING; LIU,XI; QI,TAO; YU,CHEN.
Abstract: Besides stimulating vasoconstriction, Angiotensin II is also well known in inducing reactive oxygen species and promoting inflammatory phenotype switch via its type 1 receptor. In clinic, Angiotensin II type 1 (AT1) receptor blocker like candesartan has been widely applied as an antihypertensive medication. We previous have demonstrated that a higher dose of candesartan plays a protective role after kidney injury. However, whether candesartan could exhibit anti-inflammatory effects remains unclear. Here, by stimulating isolated human embryonic kidney epithelial cells with tumor necrosis factor-α (TNF-α), we observed the anti-inflammation capacity of candesartan ex vivo. It was found that pre-treat with candesartan significantly suppressed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin II type 1 receptor blockers; Candesartan; Inflammation; Reactive oxygen species.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300701
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Effect of β-mercaptoetanol and cysteine on post-thawing quality and oxidative activity of ram sperm and on the viability of vitrified sheep embryos Arq. Bras. Med. Vet. Zootec.
Pradieé,J.; Cardoso,T.F.; Silva,E.F.; Gonçalves,A.O.; Gastal,G.D.A.; Rosa,C.E.; Mondadori,R.G.; Pegoraro,L.M.C.; Vieira,A.D.; Lucia Jr.,T..
ABSTRACT The effects of β-mercaptoethanol (BME) and cysteine on the viability and oxidative activity of ram sperm after thawing and on development in vitro and viability of vitrified sheep embryos were evaluated. Ejaculates from four rams were pooled and extended, composing six treatments: no antioxidants; 2mM BME; 5mM BME; 2mM BME and 5mM cysteine; 5mM BME and 5mM cysteine; and 5mM cysteine. Sperm motility, membrane and acrosome integrity, mitochondrial functionality, production of reactive oxygen species and total antioxidant capacity were similar across treatments (P>0.05). A medium with no antioxidant presented cleavage and blastocyst development rates (60.3% and 33.6%, respectively) similar (P>0.05) to those of a medium with 50μM BME and 600μM...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sheep embryos; Antioxidant; Reactive oxygen species; Frozen ram sperm; Vitrification.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501309
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The use of reduced glutathione (GSH) as antioxidant for cryopreserved sperm in dogs Arq. Bras. Med. Vet. Zootec.
Angrimani,D.S.R.; Nichi,M.; Brito,M.M.; Kawai,G.K.V.; Rui,B.R.; Losano,J.D.A.; Vieira,N.M.G.; Francischini,M.C.P.; Cruz,D.S.G.; Queiroz-Hazarbassanov,N.; Massoco,C.O.; Vannucchi,C.I..
ABSTRACT The aim of this study was to evaluate the effect of supplementation with different concentrations of reduced glutathione GSH (0; 5; 7.5; 10mM) in the extender for cryopreservation in dogs with evaluations performed after glycerolization (chilled) and thawing (thawed). For this purpose, we used 8 dogs and two semen collections were performed in a weekly interval, totaling 16 semen samples. The sperm were analyzed by automatic sperm motility (CASA) and flow cytometry analysis of mitochondrial potential (JC1 dye) and membrane/acrosome integrity (FITC-PI dyes). We evaluated subjectively the membrane and acrosome integrity, mitochondrial activity and DNA integrity. Seminal plasma was evaluated for lipid peroxidation (TBARS concentration). Chilled and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Cryopreservation; Antioxidant; Canine.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000200419
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Does supplementation of vitamin C, reduced glutathione or their association in semen extender reduce oxidative stress in bovine frozen semen? Arq. Bras. Med. Vet. Zootec.
Pinto,S.C.C.; Almeida,D.S.; Alves,M.B.R.; Florez-Rodriguez,S.A.; Abreu Júnior,G.S.; Alves,N. Britto e; Celeghini,E.C.C.; Laskoki,L.M.; Souza,F.A..
ABSTRACT The aim of this study was to evaluate the addition of vitamin C, reduced glutathione and the association thereof to the bovine semen cryopreservation extender. The ejaculate from nine bulls were divided into four fractions, each corresponding to a treatment, namely: control group-semen diluted with Tris-yolk extender; vitamin C group-semen diluted in Tris-yolk extender supplemented with vitamin C (2.5mmol/mL); glutathione group-semen diluted in Tris-yolk extender supplemented with reduced glutathione (2.5mmol/mL) and associated group-semen diluted in Tris-yolk extender supplemented with vitamin C (1.25mmol/mL) and reduced glutathione (1.25mmol/mL). Afterwards, the semen was packed into French straws and submitted to cryopreservation using...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Sperm membranes; Spermatozoa.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100009
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Reactive oxygen species in bovine embryo in vitro production Biocell
Dalvit,G.C.; Cetica,P.D.; Pintos,L.N.; Beconi,M.T..
Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO2: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed semen in the same culture conditions and embryo in vitro culture in IVC-mSOF at 90% N2: 5% CO2: 5% O2. ROS was determined in denuded...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Reactive oxygen species; Oxidative stress; Oocyte; Embryo; Bovine.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200009
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Preimplantation embryotoxicity after mouse embryo exposition to reactive oxygen species Biocell
Cebral,Elisa; Carrasco,Isabel; Vantman,David; Smith,Rosita.
Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 &#956;M of hydrogen peroxide (H2O2) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 &#956;M2HO2 for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001). Twenty-five &#956;M2HO2 produced inhibition of blastocyst formation (p<0.001) and 10 &#956;M...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Preimplantation embryogenesis; Toxicity; Mouse.
Ano: 2007 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100006
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Effects of CpG oligodeoxynucleotide 1826 on acute radiation-induced lung injury in mice Biol. Res.
Li,Xuan; Xu,Guoxiong; Qiao,Tiankui; Yuan,Sujuan; Zhuang,Xibing.
BACKGROUND: The radiation-induced lung injury is a common complication from radiotherapy in lung cancer. CpG ODN is TLR9 activator with potential immune modulatory effects and sensitization of radiotherapy in lung cancer. This study aimed to examine the effect of CpG ODN on acute radiation-induced lung injury in mice. METHODS AND RESULTS: The mouse model of radiation-induced lung injury was established by a single dose of 20 Gy X-rays exposure to the left lung. The results showed that the pneumonia score was lower in RT+CpG group than in RT group on 15th and 30th days. Compared with RT group, CpG ODN reduced the serum concentrations of MDA (P < 0.05) and increased the serum concentrations of SOD, GSH (P < 0.05). The serum concentration of...
Tipo: Journal article Palavras-chave: CpG ODN; Acute radiation-induced lung injury; Reactive oxygen species.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100008
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Nitric oxide reduces oxidative damage induced by water stress in sunflower plants Bragantia
Cechin,Inês; Cardoso,Guilherme Sementili; Fumis,Terezinha de Fátima; Corniani,Natália.
Drought is one of the main environmental constraints that can reduce plant yield. Nitric oxide (NO) is a signal molecule involved in plant responses to several environmental stresses. The objective of this study was to investigate the cytoprotective effect of a single foliar application of 0, 1, 10 or 100 µM of the NO donor sodium nitroprusside (SNP) in sunflower plants under water stress. Water stressed plants treated with 1μM SNP showed an increase in the relative water content compared with 0 μM SNP. Drought reduced the shoot dry weight but SNP applications did not result in alleviation of drought effects. Neither drought nor water stress plus SNP applications altered the content of photosynthetic pigments. Stomatal conductance was reduced by drought...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Photosynthesis; Reactive oxygen species; Antioxidant enzymes; Proline; Malondialdehyde.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000200200
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Resistance of oxidative stress in biofilm and planktonic cells BABT
Jakubowski,Witold; Walkowiak,Bogdan.
This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H2O2, tBOOH, menadione, SIN-1 or peroxynitrite) in a wide range of final concentrations, from 0.5 to 10mM. Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Biofilm; Bacterial colonization; Planktonic cells; Implant.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200300
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Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli BABT
Mattos,José Carlos Pelielo de; Motta,Ellen Serri da; Oliveira,Márcia Betania Nunes de; Dantas,Flávio José da Silva; Araujo,Adriano Caldeira de.
Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alkaline gel electrophoresis; Escherichia coli; Base excision repair; Stannous chloride; DNA strand breaks; Reactive oxygen species.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020
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Hydrogen Peroxide Induced Oxidative Damage on Mineral Density and Mechanical Properties of Bone BABT
Cicek,Ekrem; Cakmak,Enes.
ABSTRACT Hydrogen peroxide is a common reactive oxygen species involved in the catalytic mechanism though it is toxic to cells due to its oxidative nature. This work investigates the effects of hydrogen peroxide induced oxidative damage on bone mineral density and mechanical properties of bone which is primarily a composite material composed primarily of collagen fibers and biominerals. Sheep leg bones were exposed to hydrogen peroxide for a week. Bone mineral density was measured by using dual energy X-ray absorptiometry. Compressive modulus tests were applied to bone in order to determine mechanical properties. Our study shows that the hydrogen peroxide induced oxidative stress has negative effects on bone mineral density and stiffness. We observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bone mineral density; Compressive modulus; Hydrogen peroxide; Reactive oxygen species.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100320
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Influence of chromium salts on increased lipid peroxidation and differential pattern in antioxidant metabolism in Pistia stratiotes L BABT
Upadhyay,RishiKesh; Panda,Sanjib Kumar.
In this work, the changing effect of different concentrations (0, 0.01, 0.1, 1, 10mM) of hexavalent and trivalent chromium on different biochemical parameters along with antioxidant enzymes was investigated on water lettuce (Pistia stratiotes L.) in order to know the possible involvement of this metal in oxidative injury, besides the activities of antioxidant enzymes leading to biochemical and oxidative aberration induced by elevated concentrations. Both in roots and shoots, Cr produced a significant increase in enzymic and non-enzymic antioxidants, except in catalase (CAT) activity where a strong accumulation of hydrogen peroxide was indicated, suggesting an imposition of oxidative stress. The observation showed an uptake of chromium by P. stratiotes L....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biochemical alterations; Chromium stress; Lipid peroxidation; Oxidative stress; Pistia stratiotes L; Reactive oxygen species.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500018
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Role of nuclear factor kappa B and reactive oxygen species in the tumor necrosis factor-a-induced epithelial-mesenchymal transition of MCF-7 cells BJMBR
Dong,R.; Wang,Q.; He,X.L.; Chu,Y.K.; Lu,J.G.; Ma,Q.J..
The microenvironment of the tumor plays an important role in facilitating cancer progression and activating dormant cancer cells. Most tumors are infiltrated with inflammatory cells which secrete cytokines such as tumor necrosis factor-a (TNF-a). To evaluate the role of TNF-a in the development of cancer we studied its effects on cell migration with a migration assay. The migrating cell number in TNF-a -treated group is about 2-fold of that of the control group. Accordingly, the expression of E-cadherin was decreased and the expression of vimentin was increased upon TNF-a treatment. These results showed that TNF-a can promote epithelial-mesenchymal transition (EMT) of MCF-7 cells. Further, we found that the expression of Snail, an important transcription...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tumor necrosis factor-a; Nuclear factor kappa B; Reactive oxygen species; Snail; E-cadherin; Epithelial-mesenchymal transition.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800007
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The antioxidant action of Polypodium leucotomos extract and kojic acid: reactions with reactive oxygen species BJMBR
Gomes,A.J.; Lunardi,C.N.; Gonzalez,S.; Tedesco,A.C..
Two natural products Polypodium leucotomos extract (PL) and kojic acid (KA) were tested for their ability to scavenge reactive oxygen species (·OH, ·O2-, H2O2, ¹O2) in phosphate buffer. Hydroxyl radicals were generated by the Fenton reaction, and the rate constants of scavenging were 1.6 x 10(9) M-1 s-1 for KA and 1.0 x 10(9) M-1 s-1 for PL, similar to that of ethanol (1.4 x 10(9) M-1 s-1). With superoxide anions generated by the xanthine/hypoxanthine system, KA and PL (0.2-1.0 mg/ml) inhibited ·O2-dependent reduction of nitroblue tetrazolium by up to 30 and 31%, respectively. In the detection of ¹O2 by rose bengal irradiation, PL at 1.0 mg/ml quenched singlet oxygen by 43% relative to azide and KA by 36%. The present study demonstrates that PL showed an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polypodium leucotomos extract; Kojic acid; Antioxidant; Reactive oxygen species.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100018
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TRP channels, omega-3 fatty acids, and oxidative stress in neurodegeneration: from the cell membrane to intracellular cross-links BJMBR
Leonelli,M.; Graciano,M.F.R.; Britto,L.R.G..
The transient receptor potential channels family (TRP channels) is a relatively new group of cation channels that modulate a large range of physiological mechanisms. In the nervous system, the functions of TRP channels have been associated with thermosensation, pain transduction, neurotransmitter release, and redox signaling, among others. However, they have also been extensively correlated with the pathogenesis of several innate and acquired diseases. On the other hand, the omega-3 polyunsaturated fatty acids (n-3 fatty acids) have also been associated with several processes that seem to counterbalance or to contribute to the function of several TRPs. In this short review, we discuss some of the remarkable new findings in this field. We also review the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TRP channels; Calcium; Omega-3 fatty acids; Reactive oxygen species; G-protein coupled receptors; Neurodegeneration.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100003
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