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| Registros recuperados: 36 | |
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| Hernández Deheza, María Guadalupe. |
| En Yurécuaro, Michoacán, en el ciclo de cultivo verano otoño 2009 y 2011 se encontraron en cultivos de chile síntomas de variegado, nervaduras cloróticas, deformación de la lámina foliar y fruto, albinismo y escaldadura, síndrome al cual se le denominó “variegado del chile”. A simple vista, esta serie de síntomas indicaban que el problema era causado por virus fitopatógenos; sin embargo, en ensayos previos se detectó a Candidatus Liberibacter solanacearum, por lo que el objetivo del presente trabajo fue determinar si esta bacteria es el agente causal del variegado del chile. Mediante transmisión por injerto, transmisión con el vector Bactericera cockerelli y reacción en cadena de la polimerasa con el par de iniciadores OA2/CLi.po.F y OI2, se confirmo que... |
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Palavras-chave: Candidatus Liberibacter; PCR; Secuenciación; Sequencing; Entomología y Acarología; Doctorado. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1844 |
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| Chibeu,Andrew; Lavigne,Rob; Mattheus,Wesley; Matthijs,Sandra; Cornelis,Pierre; Volckaert,Guido. |
| We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries. |
| Tipo: Journal article |
Palavras-chave: Cosmid; PRG930cm; Pseudomonas; Sequencing. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200010 |
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| Sanna,Daria; Lai,Tiziana; Francalacci,Paolo; Curini-Galletti,Marco; Casu,Marco. |
| Monocelis lineata consists of a complex of sibling species, widespread in the Mediterranean and Atlantic Ocean. Previous genetic analysis placed in evidence at least four sibling species. Nevertheless, this research was not conclusive enough to fully resolve the complex or to infer the phylogeny/phylogeography of the group. We designed specific primers aiming at obtaining partial sequences of the mtDNA gene Cytochrome c Oxidase subunit I (COI) of M. lineata, and have identified 25 different haplotypes in 32 analyzed individuals. The dendrogram generated by Neighbor-Joining analysis confirmed the differentiation between Atlantic and Mediterranean siblings, as well as the occurrence of at least two Mediterranean sibling species. Thus validated, the method... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microturbellaria; Sequencing; Sibling species; Phylogeography; Phylogeny. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400031 |
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| Coêlho,Mariza M.; Ferreira-Nozawa,Monica S.; Nozawa,Sérgio R.; Santos,André L.W.. |
| Endophytic bacteria from three arboreal species native to the Amazon (Carapa guianenses, Ceiba pentandra, and Swietenia macrophylla), were isolated and identified, through partial sequencing of the 16S rRNA encoding gene. From these, 16 isolates were obtained, although, when compared to sequences deposited in GenBank, only seven had produced identifiable fragments. Bacillus, Pantoea and two non-culturable samples were identified. Results obtained through sequence analysis revealed low genetic diversity across the isolates, even when analyzing different species and plant structures. This is the first report concerning the isolation and identification of endophytic bacteria in these plant species. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Sequencing; Bacillus; Pantoea. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400021 |
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| Gombac, Mitja; Kusar, D.; Ocepek, M.; Pogacnik, Milan; Arzul, Isabelle; Couraleau, Yann; Jenclc, V.. |
| Among 1280 cultured and wild adult Mediterranean mussels, Mytilus galloprovincialis, collected over a 1-year surveillance period from the Slovene Adriatic Sea, 0.3% were histologically positive for the presence of Marteilia spp. The infection was concentrated in winter. Employing the molecular methods of PCR, cloning, DNA restriction and sequencing, only Marteilia refringens type M was detected in all the infected mussels. Although all life-cycle stages of M. refringens severely infected digestive glands, only sporadic disruption of epithelial cells of digestive tubules and focal destruction of digestive tubules were observed in the infected mussels. This was the first detection of M. refringens in M. galloprovincialis from the Slovene Adriatic Sea with... |
| Tipo: Text |
Palavras-chave: Prevalence; Marteilia refringens type M.; Mediterranean mussels Mytilus galloprovincialis; Sequencing; Severe infection; Slovene Adriatic Sea. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00165/27640/26064.pdf |
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| Shinn, A.P.; Banks, B.A.; Tange, N.; Bron, J.E.; Sommerville, C.; Aoki, T.; Wootten, R.. |
| Genetic differentiation within the salmon louse Lepeophtheirus salmonis (Krøyer, 1837), was investigated by the sequencing of specific nucleotide regions. Partial sequences of the 18S ribosomal RNA gene and the ribosomal internal transcribed spacer (ITS-1) region from single sea lice were amplified by the polymerase chain reaction (PCR). Lice were collected from wild and farmed Atlantic salmon ( Salmo salar L., 1758) from nine selected localities around the Scottish coastline. A 0.9kb fragment of the 18S ribosomal RNA gene was amplified and compared for several samples of lice which showed no observable differences between the lice from different collection sites confirming the absence of cryptic species. The 454 nucleotide ITS-1 sequence showed... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Sea louse; Copepod; Crustacea; Atlantic salmon; Sequencing; 18S ribosomal DNA; Internal transcribed spacer; Population genetics. |
| Ano: 2000 |
URL: http://www.repository.naturalis.nl/record/534343 |
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| Farrugia, A.F.; Pla, C.; Viñas, J.; Tawil, M.Y.; Hattour, A.; de la Serna, J.M.. |
| None of the previous genetic analysis found significant differences within the Mediterranean. However, a more exhaustive analysis is needed inside this sea. In this sense, we have analyzed the mitochondrial control region sequence variability of three Mediterranean locations: Libya (n = 22), Tunisia (n = 23) and Malta (n = 12). Additionally a sample from the Gulf of Cadiz (n = 24) in the East Atlantic Ocean was also included in the study. The AMOVA analysis revealed slightly (Fst = 0.013) but significative genetic differences (P < 0.000) for the Malta location, probably due to its small sample size (n = 12). In the other hand, when this location is removed from the analysis, no differences where found among the three remaining location (Fst = 0,005; P =... |
| Tipo: Journal Contribution |
Palavras-chave: Atlantic ocean; Mitochondrial DNA; Bluefin tuna; Sequencing; Genetic characterization; Mediterranean sea; Tuna fisheries. |
| Ano: 2003 |
URL: http://hdl.handle.net/1834/792 |
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| Villarreal,LYB; Brandão,PEB; Chacón,JLV; Doretto Junior,L; Ito,N; Gama,NS; Ishizuka,MM; Luchese,A; Buchala,F; Astolfi-Ferreira,CS; Ferreira,AJP. |
| Avian Infectious Laryngotracheitis, caused by Infectious Laryngotracheitis Virus (ILTV), has been reported for decades in Brazilian laying and broiler flocks. More recently, outbreaks have occurred in São Paulo State. This study reports the application of PCR and DNA sequencing targeted to the p32 gene of ILTV using laying chicken samples from Bastos, São Paulo, Brazil. Three out of four field samples were positive by PCR. DNA sequencing of two samples evidenced homology of the amplified fragments with the p32 gene of ILTV. The results definitely confirmed the presence of ILTV in the birds during the outbreak. Further studies are needed to establish the sources of infection and to determine whether the detected virus was originated from vaccine or field... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avian infectious laryngotracheitis; Brazil; P32; PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000400011 |
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| Silva,Mirian de Sousa; Brommonschenkel,Sérgio Hermínio; Faria,José Márcio Rocha; Borges,Eduardo Euclydes de Lima e. |
| The objective of this study was to partially characterize some genes involved in the desiccation tolerance of the embryonic axis of Melanoxylon brauna seeds subjected, or not, to oven fast-drying. Seeds were initially dried rapidly in an oven at 40 ºC, 50 ºC, 60 ºC, 70 ºC, and 80 °C, for 24, 48 and 72 h and then subjected to germination tests and moisture content determination. Degenerate primers were designed for 19 genes. The CDNA was used as a template for PCR amplifications using the degenerate primers, and the PCR products obtained were purified, cloned and sequenced. The seeds showed a gradual reduction in percent germination with increasing temperature and drying time. Nucleotide sequences of the cloned fragments related to genes CAT1, SPS1, Abi5,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Water; Stress; Drying; Sequencing. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222012000100004 |
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| Ruiz,Ana Zuley. |
| The objective of the present study was to report the molecular cloning and determination of the sequences N-methyl-D-aspartate subunit-1 receptor (NMDAR-1) in the hypothalamus of sheep. The re-amplified DNA template for NMDA -1 from the hypothalamus of male sheep was cloned using pBluescript-Sk-plasmids (pBSK, 2958 bp). Purified plasmids containing the NMDA receptor c-DNA were sequenced using the dye-terminator chemistry at the University of Nebraska-Lincoln, Nebraska, USA. Results were entered into the National Center for Biotechnology Web site as accession number AY434689. The sequence of Ovis aries (sheep) NMDAR-1 mRNA from hypothalamus of male sheep has 94-97% homology with Homo sapiens, 94-100% homology with Sus scrofa, and 88-90% homology with Rattus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cloning; NMDA receptor; Ovis aries; Ram; Sequencing. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000400183 |
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| Rocha,Kelly Cristina Gonçales; Marubayashi,Julio Massaharu; Navas-Castillo,Jesús; Yuki,Valdir Atsushi; Wilcken,Carlos Frederico; Pavan,Marcelo Agenor; Krause-Sakate,Renate. |
| Bemisia tabaci (Genn.) is one of the most important pests in cultivated areas of vegetables and ornamental crops around the world. Based on the mitochondrial cytochrome oxidize I (mtCOI) sequence, there is evidence that B. tabaci should be considered a cryptic species complex of 11 groups containing 24 species. Two of the groups, Middle East-Asia Minor 1 and Mediterranean include biotypes B and Q, respectively. In this study we evaluated the mtCOI sequence of B. tabaci populations collected in sites of the state of São Paulo, Brazil. Using PCR-RFLP with Taq I, a typical biotype B profile was obtained for all specimens. Based on the comparison with mtCOI reference sequences we found four haplotypes all belonging to the Middle East-Asia Minor 1. They... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RFLP; Cytochrome oxidase I gene; Sequencing; Whiteflies. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000100018 |
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| Colariccio,Addolorata; Eiras,Marcelo; Chaves,Alexandre L. R.; Harakava,Ricardo; Chagas,César M.. |
| In the regions of Campinas and Sumaré, São Paulo, Brazil, hidroponically grown crops of Lettuce (Lactuca sativa) cv. Verônica, which showed virus-like symptoms were examined by electron microscope, biological, serological and molecular tests. Pleomorphic, enveloped particles (80-100 nm in diameter) were always detected in these samples. Experimentally inoculated host plants, including lettuce, reacted with tospoviruses-induced symptoms. Some differences were observed in Gomphrena globosa, which reacted by showing local lesions and systemic mosaic. Two isolates of Tomato chlorotic spot virus (TCSV) were identified by DAS-ELISA and by RT-PCR. The sequencing and alignment of the RT-PCR coat protein amplified fragments have indicated a high degree of homology... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Tospovirus; Serology; RT-PCR; Sequencing. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582004000300012 |
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| Herrera Vásquez,José Angel; Jaén Sanjur,José Natividad; Zachrisson Salamina,Bruno Alexis; Rubio Miguélez,Luis; Barba Alvarado,Anovel Amet; Aguilera Cogley,Vidal Antonio; Valdespino,Randy Atencio; Torregrosa 0,Luis Galipienso; Mejía Franco,Luis Carlos. |
| Abstract The main open-field and greenhouse producer regions of tomato in Panama (Los Santos, Herrera, Veraguas and Chiriquí provinces) were surveyed to determine the occurrence and distribution of Bemisia tabaci and Trialeurodes vaporariorum during the growing seasons of 2017 and 2018. Twenty-two plots were surveyed, and DNA extracts of 112 adult whiteflies were analyzed by multiplex PCR, using a set of specific primers of B. tabaci MEAM1, B. tabaci NW, and T. vaporariorum. DNA extracts corresponding to 57 individual whiteflies (50.9 %) rendered PCR products of expected size with B. tabaci-specific primers (37 belonged to B. tabaci MEAM1 and 20 to B. tabaci NW), whereas the remaining DNA extracts corresponding to 55 individual whiteflies (49.1 %) rendered... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Altitude; PCR; Phylogenetic; Sequencing; Weather conditions; Whiteflies. |
| Ano: 2022 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122022000100096 |
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| Vieira, Viviane Cristina; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Alves, Pedro Luís da Costa Aguiar; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Picchi, Simone Cristina; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Lemos, Manoel Victor Franco; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista; Sena, Janete Apparecida Desidério; Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista. |
| Digitaria species are sugar cane crop weeds in Brazil and are being controlled with herbicides, although there are some reports of control failure, notably to the triazine group. Molecular techniques are recommended to analyze the genetic variability in weeds. RAPD (Random Amplified Polymorphic DNA), PCR-RFLP (Polymerase Chain Reaction – Restriction Fragment Length Polymorphism) and, in combination with sequencing, allow the localization of resistance genes, as well as possible mutations related to the onset of resistant individuals in some species. Thus, the objective of this work was to characterize ten accessions of Digitaria spp. by RAPD and PCR-RFLP markers, to sequence a conserved region of the psb A gene and evaluate the accessions response to... |
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Palavras-chave: Digitaria; PsbA gene; RAPD; Sequencing; Triazine; Weed Digitaria; PsbA gene; RAPD; Sequencing; Triazine; Weed. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3841 |
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| Deef, Lamiaa Elsayed Mokhtar. |
| This is the first study to detection the genetic relationship between Porphyrio alleni and four Rallidae species: Fulica atra, Gallinulla angulata, Gallinulla chloropus and Porphyrio porphyrio. Mitochondrial cytochrome oxidase subunit I (COI) sequences were used as an effective marker in this study. DNA of Rallidae species were extracted, amplified using polymerase chain reaction (PCR) and then sequenced. The results obtained from information based on COI sequences revealed that Gallinulla angulata and Gallinulla chloropus fall into two separate clades and they are not monophyletic. This suggests that, two moorhens could not be laid into the same genus. In addition, Porphyrio porphyrio was included in the same genus with Porphyrio alleni but they were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; Gruiformes; PCR; Phylogeny; Sequencing; Species.. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/37184 |
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| Registros recuperados: 36 | |
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