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| Registros recuperados: 240 | |
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| Solari,Alberto J.; Rahn,Monica I.; Saura,Alicia; Lujan,Hugo D.. |
| The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei. This full division occurs in a dorsal-ventral direction, with the resulting nuclear pairs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Giardia; Primitive mitosis; Fine structure of mitosis; Protist mitosis; Cell differentiation. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300004 |
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| Vigliocco,A.; Bonamico,B.; Alemano,S.; Miersch,O.; Abdala,G.. |
| In the present paper we study the possible biological relevance of endogenous jasmonic acid (JA) and exogenous salicylic acid (SA) in a plant-microbial system maize-virus. The virus disease "Mal de Río Cuarto" is caused by the maize rough dwarf virus - Río Cuarto. The characteristic symptoms are the appearance of galls or "enations" in leaves, shortening of the stem internodes, poor radical system and general stunting. Changes in JA and protein pattern in maize control and infected plants of a virus-tolerant cultivar were investi-gated. Healthy and infected-leaf discs were collected for JA measurement at different post-infection times (20, 40, 60 and 68 days). JA was also measured in roots on day 60 after infection. For SDS-PAGE protein analysis, leaf... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Maize; Virus disease; Jasmonic and salicylic acids. |
| Ano: 2002 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300007 |
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| Lee,Chang Seok; Shin,Yong Jae; Won,Cheolhee; Lee,Yun-Song; Park,Chung-Gyu; Ye,Sang-Kyu; Chung,Myung-Hee. |
| Cyclooxygenase-2 (COX-2) is a key inflammatory response molecule, and associated with many immune functions of monocytes/macrophages. Particularly, interferon gamma (IFNgamma)-induced COX-2 expression appears in inflammatory conditions such as viral infection and autoimmune diseases. Recently, statins have been reported to show variable effects on COX-2 expression, and on their cell and species type dependences. Based on the above description, we compared the effect of simvastatin on IFNgamma-induced COX-2 expression in human monocytes versus murine macrophages. In a result, we found that simvastatin suppresses IFNgamma-induced COX-2 expression in human THP-1 monocytes, but rather, potentiates IFNgamma-induced COX-2 expression in murine RAW264.7... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Monocyte; Macrophage; INFgamma; STAT1/3; SOCS1/3. |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200005 |
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| Kohli,Alicia; Pezzotto,Stella M.; Garcia,Graciela; Poletto,Leonor C.. |
| The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin, periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results with the erosion technique. Thirty human teeth were employed in the trial; after their extraction the pulp was fixated, the pieces... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulp dentine organ; Odontoblast; Odontoblast process. |
| Ano: 2011 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000200003 |
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| Zou,Yi; Zhong,Wenping. |
| PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity. Full length PEPP2 associated with both plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microtubule; Membrane; Phosphoinositide; Microviscosity; Wortmannin. |
| Ano: 2012 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300005 |
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| Acosta de Pérez,Ofelia; Leiva de Vila,Laura; Peichoto,María Elisa; Maruñak,Silvana; Ruíz,Raquel; Teibler,Pamela; Gay,Carolina; Rey,Laura. |
| Philodryas olfersii is found in South America, from Amazonas to Patagonia. It is important to characterize the venom of P. olfersii, who inhabits the North-East region of Argentina, since snake venoms are known to exhibit considerable variability in composition and biological activities. In this work, mice weighing 18-20 g (n = 4 for each experimental group) were used. For the edematogenic activity mice were injected s.c. in the right foot pad with 50 ml of solutions containing different amounts of venom, whereas the left foot pad was injected with 50 ml of PBS. Two hours after injection mice were killed by cervical dislocation and both feet were cut off and weighed individually. For the myotoxic activity mice were injected i.m. with 100 ml of solutions... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Philodryas olfersii; Edematogenic activity; Myotoxic activity. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300007 |
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| Castejón,Orlando J.. |
| The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fibers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells have functional ionotropic glutamate receptors that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calbindin; Synapsin-PSD95; GluR1; CamKII; N-cadherin. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200003 |
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| Faraldo,A. C.; Sá-Nunes,A; Faccioli,L. H.; Del Bel,E. A.; Lello,E. |
| Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected (SIL) larvae of the blowfly Chrysomya... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NO synthase; Nitric oxide; NADPH-diaphorase; Chrysomya megacephala; Blowfly; Tissues. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000200002 |
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| Pronsato,Lucia; La Colla,Anabela; Ronda,Ana Carolina; Milanesi,Lorena; Boland,Ricardo; Vasconsuelo,Andrea. |
| Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Over-subculturing changes; Aged muscle cultures; Mitochondrial DNA. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100001 |
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| Nome,Claudia Fernanda; Nome,Sergio Fernando; Guzmán,Fabiana; Conci,Luis; Laguna,Irma Graciela. |
| Among diseases reported worldwidely for sweet potato (Ipomoea batatas (L) Lam) crop, one of the most frequent is the Sweet potato virus disease (SPVD), caused by sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus (SPFMV) co-infection. In Argentina, there exists the sweet potato chlorotic dwarf (SPCD), a sweet potato disease caused by triple co-infection with SPCSV, SPFMV and sweet potato mild speckling virus (SPMSV). Both diseases cause a synergism between the potyviruses (SPFMV and SPMSV) and the crinivirus (SPCSV). Up to date, studies carried out on the interaction among these three viruses have not described their localization in the infected tissues. In single infections, virions of the crinivirus genus are limited to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sweet potato chlorotic stunt virus; HSP70h; In situ hybridization; Crinivirus; Sweet potato. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452007000100003 |
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| López-Herrera,A.; Ruiz-Sáenz,J.; Góez,Y.P.; Zapata,W.; Velilla,P.A.; Arango,A.E.; Urcuqui-Inchima,S.. |
| To determine whether fibroblasts from Blanco Orejinegro cattle, exhibit any level of resistance to infection against vesicular stomatitis virus (VSV) serotypes Indiana (VSV-I) or New Jersey (VSVNJ), 30 fibroblast cultures were phenotyped to evaluate their resistance/susceptibility. Thirty three % of Blanco Orejinegro fibroblast cultures were classified as very resistant, 50% as resistant, and 17% as susceptible to VSV-I infection, whereas 20% were classified as very resistant, 50% as resistant and 30% as susceptible to VSV-NJ infection. Therefore, there appears to be a large variation in phenotypic polymorphism among the fibroblasts to infection by VSV. To elucidate the mechanisms responsible for this diversity, we searched for a possible relationship... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vesicular stomatitis virus; Apoptosis; Resistance; Bovine fibroblasts (Source: NLM). |
| Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000200007 |
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| Ravaglia,Mario Aldo; Maggese,María Cristina. |
| Synbranchus marmoratus (Synbranchidae), commonly known as the swamp eel, is a protogynous diandric teleost fish widely distributed throughout South America. The purpose of this work was to study the ovarian anatomy and to describe oocyte developmental stages in the swamp eel, Synbranchus marmoratus. S. marmoratus has a unique sacular ovary. It is covered by a conspicuous muscular wall, probably involved in an egg-releasing system acting as a peristaltic-like mechanism. The internal ovarian anatomy shows a U-shaped ovarian lamella delimiting a dorsal ovarian lumen. The microscopic study shows evidence of the existence of a germinal epithelium in the inner surface of the lamella, which contains germinal cells, pre-follicular cells and epithelial cells. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Synbranchidae; Ovarian anatomy; Oogenesis; Micropyle. |
| Ano: 2002 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452002000300003 |
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| Caro,Luis A.; Santecchia,Natalia; Marinangeli,Pablo A.; Curvetto,Néstor R.; Hernández,Luis F.. |
| The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agrobacterium rhizogenes; Algarrobo; Hairy roots; Indole-3-butyric acid; Nothofagus nervosa; Prosopis chilensis; Raulí; Rooting. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300002 |
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| Registros recuperados: 240 | |
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