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| Registros recuperados: 233 | |
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| Oliveira,Luciana do Valle Rego; Faria,Ricardo Tadeu de; Ruas,Claudete de Fátima; Ruas,Paulo Maurício; Santos,Melissa de Oliveira; Carvalho,Valdemar P.. |
| In this work, RAPD molecular markers were used to access the genetic variability and to study the inter and intraespecifc relationship in a group of 37 species, including 56 individuals. A total of 15 RAPD primers were selected for DNA amplification. From a total of 221 bands analyzed, 209 (95%) were polymorphics. The level of interespecifc genetic similarity ranged from 37% between Catasetum complanatum and Catasetum laminatum to 83% between Catasetum triodon and Catasetum uncatum. The intraspecifc genetic similarity varied 88% for the individuals of Catasetum triodon to 93% between the individuals of Catasetum atratum and Catasetum macrocarpum. These results would contribute to understand the genetic relationship in Catasetum, to define the strategies to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catasetum; Molecular marker; Orchid; RAPD; Genetic similarity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200017 |
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| Saleh,Basel. |
| The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002 |
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| Torezan,José Marcelo Domingues; Souza,Rogério Fernandes de; Ruas,Paulo Maurício; Ruas,Claudete de Fátima; Camargo,Elvis Hassmann; Vanzela,André Luís Laforga. |
| RAPD was used to access the genetic variability in Aspisdosperma polyneuron, a long-lived, late-reproducing tropical tree, and highly important for the Atlantic Forest. RAPD profiles from adults (pre-fragmentation, >300 years old) and seedlings (post-fragmentation, <<50 years old) were analyzed. Results showed a decrease of genetic polymorphism of post-fragmentation cohorts in small fragments and higher genetic diversity within population. The genetic diversity distribution suggested the establishment of fragments as protected reserves, and the transference of seedlings among fragments for conservation of A. polyneuron. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspidosperma; DNA markers; Forest fragmentation; Genetic variability; Peroba-rosa; RAPD. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200002 |
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| Lopes-da-Silva,Marcelo; Vieira,Luiz Gonzaga Esteves. |
| The aim of this work was to analyze the clonal diversity variation in Schizaphis graminum during a complete phenological cycle of black oats (Avena strigosa). RAPD markers were used for detection of DNA polymorphisms of each clonal lineage, derived from a single clone collected weekly during a period of four months, in a crop field of black oats, Londrina, Paraná, Brazil. The monthly genotypic diversity was estimated by Shannon Information Index (H). Only four genotypes were distinguished from 122 specimens, with one of them overly predominant in all sampling dates (>60%). Another genotype, apparently a later colonizer, rapidly reached greater frequency than other genotypes on the second and third month. The results of this work suggested that temporal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; RAPD; Temporal scale. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400021 |
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| Zeshan,Ali; Afzal,Muhammad; Alghamdi,Salem S.; Kettener,Karien; Ali,Mubashar; Mubushar,Muhammad; Ahmad,Shakeel. |
| ABSTRACT The presence of geneticdiversity is of great importance in improving wheat traits and developing strategies for optimal conservation of germplasm. Genetic diversity was assessed among common wheat cultivars using RAPD (Random Amplified Polymorphic DNA) markers at the Center of Agriculture, Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad. RAPD primers were used among 14 Pakistani wheat cultivars, to screen the progenies and for the identification of the genes of interest. The polymorphic information content (PIC), was measured as the percentage of polymorphic fragments for all primers. A total of 583 bands(84% polymorphic) in all 14 wheat cultivars was amplified and discriminated all the wheat genotypes. The number of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wheat; RAPD; Genetic diversity; PCoA; Cluster analysis. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100432 |
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| Vargas,Lúcia Rosane Bertholdo; Rossato,Marcelo; Ribeiro,Rute Terezinha da Silva; Barros,Neiva Monteiro de. |
| The characterization of entomopathogenic microorganisms is important for the selection of more effective strains for use in integrated pest-control programs. Five Nomuraea rileyi strains (SA86101, GU87401, SR86151, CG128 and VA9101) were characterized using random amplified polymorphic DNA (RAPD) analysis, virulence studies and assessment of chitinolytic and proteolytic activity. RAPD analysis divided the strains into two groups with a similarity coefficient of 0,76%, group 1 consisting of strains SA86101, GU87401 and SR86151 and group 2 of strains CG128 and VA9101. The LT50 varied from 165h with strain VA9101 to 246h with strain GU87401. Chitinolytic and proteolytic activity of the fungi after 144h growth in minimal medium were tested using colloidal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nomuraea rileyi; Anticarsia gemmatalis; Chitinase; Protease; RAPD; Virulence. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100003 |
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| Diniz,Leandro Eugênio Cardamoni; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Torres,Fabrício Medeiros; Ruas,Eduardo Augusto; Santos,Melissa de Oliveira; Sera,Tumoru; Ruas,Paulo Maurício. |
| The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Genetic variability; RAPD; Restriction endonucleases. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002 |
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| Grazziotin,Felipe; Echeverrigaray,Sergio. |
| The genetic relationships among 11 species of Bothrops found in Brazil were determined using random amplified fragment (RAPD) information. A total of 239 amplified bands were scored using 20 aleatory primers. Most of the bands (88.7%) were polymorphic. Phylogenetic analysis of the data determined three similarity groups within Bothrops: Group I- B. alternatus, B. neuwiedi, B. cotiara, and B. jararacusu; Group II- B. insularis, B. jararaca, and B. erythromelas; and Group III- B. moojeni, B. leucurus, and B. atrox. These groups coincided with those obtained by other methods, indicating that RAPD's could be a useful tool for the evaluation of genetic relationships at the interspecific level. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops; RAPD; Molecular markers; Genetic relationship. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300005 |
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| Stringari,Danyelle; Glienke,Chirlei; Christo,Daniel de; Maccheroni Jr.,Walter; Azevedo,João Lucio de. |
| RAPD markers were used to investigate the distribution of genetic variability among a group of Guignardia citricarpa, G. mangiferae, and Phyllosticta spinarum isolates obtained from several hosts in Brazil, Argentina, Mexico, Costa Rica, Thailand, Japan, United States and South Africa. Pathogenic isolates G. citricarpa Kiely (anamorph form P. citricarpa McAlp Van Der Aa) are the etiological agent of the Citrus Black Spot (CBS), a disease that affects several citric plants and causes substantial injuries to the appearance of their fruits, thus preventing their export. Several previous studies have demonstrated the existence of an endophytic species with high morphological similarity to the causal agent of CBS that could remain latent in the same hosts.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Guignardia; SCARS; Citrus Black Spot; Citrus; Diagnosis; RAPD. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500002 |
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| Sahyun,Sandra Aparecida; Ruas,Eduardo Augusto; Ruas,Claudete de Fátima; Medri,Cristiano; Souza,José Roberto Pinto de; Johansson,Loana Aparecida Pereira da Silva; Miranda,Luíz Vicente; Ruas,Paulo Maurício. |
| Three populations of Maytenus aquifolium from Monte Alegre farm, Telemaco Borba county, Paraná, Brazil were analyzed by RAPD markers. A total of 13 primers were applied wich yielded 283 well amplified markers in all the studied populations (Mortandade, Vila Preta and Trinita), producing different values of gene diversity and polymorphic loci. The analysis of molecular variance (AMOVA) indicated that 21.77% of the genetic variation was among the population. Pairwise F ST analysis showed that the most divergent populations were closer geographically, demonstrating that other factors such as different soil types could explain this variation. Bayesian analysis for K number of clusters and the Principal Coordinate indicated that these three populations were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conservation; Genetic diversity; Maytenus aquifolium; RAPD; Populations. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500005 |
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| Gimenez,Danilo Lucano; Mota,Lígia Souza Lima Silveira da; Curi,Rogério Abdallah; Rosa,Guilherme Jordão de Magalhães; Gimenes,Marcos Aparecido; Lopes,Catalina Romero; Lucca,Edmundo José de. |
| A presente investigação teve como objetivos: analisar animais presentes em diferentes criações de javalis no estado de São Paulo, com o intuito de auxiliar a identificação de javalis "puros" assim como javalis híbridos provenientes do cruzamento com o suíno doméstico, para tanto foram utilizadas avaliação do fenótipo dos animais, análises citogenéticas e da técnica molecular de RAPD (Random Amplified Polymorphic DNA).O estudo do número de cromossomos nas células diplóides em 104 animais destinados a análise citogenética e fenotípica, revelou polimorfismo de 2n=36, 37 e 38 cromossomos. Por meio da técnica de bandamento GTG foi possível identificação da translocação Robertsoniana entre os cromossomos 15 e 17 como responsável por esse polimorfismo. Todavia,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Javali; Híbrido; Citogenética; RAPD; Fenótipos. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962003000200009 |
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| Dizbay,Murat; Kalkanci,Ayse; Sezer,Busra Ergut; Aktas,Firdevs; Aydogan,Sibel; Fidan,Isil; Kustimur,Semra; Sugita,Takashi. |
| We investigated a nosocomial cluster of four Candida parapsilosis fungemia episodes that occurred in a neurological intensive care unit over a two-week period. The four infected patients had received parenteral nutrition through central lines, and all four had catheter-related candidemia. All of the isolates were susceptible to all of the antifungals tested, including amphotericin B, fluconazole, voriconazole, and caspofungin. They had strictly related fingerprints, based on randomly amplified polymorphic DNA analysis. Additional DNA sequencing data revealed that they were same strain. Although no isolate of Candida parapsilosis was recovered from other clinical, surveillance, or environmental samples, nosocomial spread of this yeast ceased, following the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candidemia; Candida parapsilosis; Nosocomial outbreak; Intensive care unit; RAPD; DNA sequencing. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500010 |
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| Romanelli,RMC; Clemente,WT; Lima,SSS; Rezende,EM; Martinho,GH; Paiva,LFR; Neves,FAC; Madeira,JGC; Amâncio,GCS; Lima,AS; Faria,LC; Coutinho,RL. |
| Methicillin-resistant Staphylococcus aureus (MRSA) infections frequently complicate the post-operative course of transplant recipients, and despite nasal carriage and endemic colonization, MRSA outbreaks are not commonly described. This study reports a case of MRSA outbreak and discusses infection control measures and recommendations for this situation. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Outbreak; Surgical site infection; Liver transplantation; RAPD. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100011 |
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| Freire,Laurineide Lopes de Carvalho; Costa,Ana Bolena Lima da; Góes,Larissa Brandão; Oliveira,Neiva Tinti de. |
| Five mutants (MaE10, MaE27, MaE24, MaE41 e MaE49) of Metarhizium anisopliae wild strain E9 were analysed for DNA profile through the RAPD technique and for changes in total protein content by spectrophotometry, polyacrylamide gel electrophoresis and densitometry. The pattern of RAPD markers showed genetic polymorphism among the strains: out of twenty primers seven were selected, producing 113 bands. Forty seven bands were present in all strains (41.6% of monomorphic bands) and 66 showed polymorphism (58.4%). The mean coefficient of similarity among all strains was 0.75 (75%). The total protein content varied, staining in the interval of 6.0-8.0 µg/µl. The electrophoresis analysis, through zymogram and protein fraction profiles by densitometry, allowed the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metarhizium anisopliae; Mutants; RAPD; Total protein. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200004 |
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| Registros recuperados: 233 | |
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