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A Functional Study of Transforming Growth Factor-Beta from the Gonad of Pacific Oyster Crassostrea gigas ArchiMer
Corporeau, Charlotte; Groisillier, Agnes; Jeudy, Alexandra; Barbeyron, Tristan; Fleury, Elodie; Fabioux, Caroline; Czjzek, Mirjam; Huvet, Arnaud.
The transforming growth factor (TGF)-beta superfamily is a group of important growth factors involved in multiple processes such as differentiation, cell proliferation, apoptosis and cellular growth. In the Pacific oyster Crassostrea gigas, the oyster gonadal (og) TGF-beta gene was recently characterized through genome-wide expression profiling of oyster lines selected to be resistant or susceptible to summer mortality. Og TGF-beta appeared specifically expressed in the gonad to reach a maximum when gonads are fully mature, which singularly contrasts with the pleiotropic roles commonly ascribed to most TGF-beta family members. The function of og TGF-beta protein in oysters is unknown, and defining its role remains challenging. In this study, we develop a...
Tipo: Text Palavras-chave: Transforming growth factor-beta; Bacterial expression; In vivo antibody inhibition; Reproduction; Crassostrea gigas; Marine bivalve.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00042/15371/13438.pdf
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Impact of atrazine on somatic aneuploidy in cupped oysters, Crassostrea gigas ArchiMer
Bouilly, Karine; Leitao, Alexandra; Mccombie, Helen; Chaves, R.; Guedes Pinto, H; Boudry, Pierre; Lapegue, Sylvie.
In the Pacific oyster, Crassostrea gigas hypodiploid aneuploid cells with 2n=19, 18 or 17 chromosomes have regularly been reported. There is a negative correlation between somatic aneuploidy and growth rate in oysters (Leitão et al., 2001a) as well as evidence for a genetic basis (Leitão et al., 2001b). Furthermore, non-random chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique (Leitão et al., 2001c). This poster summarises different studies used to investigate the effects of the herbicide atrazine on the level of aneuploidy in this species
Tipo: Text Palavras-chave: Genetic; Aneuploidy; Toxicology; Impact; Atrazine; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3489.pdf
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Changes in biochemical and hemocyte parameters of the pacific oysters Crassostrea gigas fed T-Iso supplemented with lipid emulsions rich in eicosapentaenoic acid ArchiMer
Delaporte, Maryse; Chu, Fu-lin; Langdon, Chris; Moal, Jeanne; Lambert, Christophe; Samain, Jean-francois; Soudant, Philippe.
The aim of this study was to assess the effect of dietary eicosapentaenoic acid (20:5n-3) on hemocyte parameters such as hemocyte concentration, phagocytosis, and non-stimulated reactive oxygen species (ROS) production in Pacific oysters Crassostrea gigas, as well on proximate biochemical and fatty acid compositions. One-year-old oysters (C. gigas) were fed T-Isochrysis aff. galbana(T-Iso), which is low in 20:5n-3, either alone or with supplements of a lipid emulsion rich in 20:5n-3 at 1%, 10% or 50% (dry weight of the algal ration) for up to 7 weeks. Changes in gill fatty acid composition demonstrated that the lipid emulsion was well ingested by oysters during the dietary conditioning. Biochemical analysis indicated that oysters fed supplements of 50%...
Tipo: Text Palavras-chave: Polyunsaturated fatty acids; Immunology; Hemocytes; Eicosapentaenoic acid 20 : 5n 3; Crassostrea gigas; Bivalves.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2604.pdf
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Caractérisation précoce de la mortalité des lignées divergentes de Crassostrea gigas (G2) ArchiMer
Degremont, Lionel; Soletchnik, Patrick; Doner, Anne.
2001 shows how important the "R" and "S" biological families are for experimentation as well as the need to "preserve" these families from mortality during the first year. The "experimental nursing" conditions do not allow the conservation of dozens of families with many members for 6 to 8 months. Precocious characterisation at around 4-5 months enables a first selection of the biological material, as well as a first answer to the "in lab" genetical selection of the families. After the first results in 2001 (Céline de Ambroggi, LCPC, L. Degremont), two "precocious characterisation of mortality" experiments are carried out in 2002 on the divergent lineage of some genetical families (LGP production). Two 15 day long experiments are conducted in la Tremblade...
Tipo: Text Palavras-chave: Mortalités; Sélection; Physiologie; Genetique; Crassostrea gigas; Huîtres.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3406.pdf
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Métabolisme de l'huître Crassostrea gigas stockée dans des dégorgeoirs conchylicoles, à différentes densités et saisons ArchiMer
Bougrier, Serge; Masson, Daniel; Geairon, Philippe; Fouche, Dominique; Leroy, Anne; Faury, Nicole; Ratiskol, Jacqueline.
One of the problems faced by oyster farmers in France is the immediate or delayed mortality of oysters in the storage tanks prior to shipping. This may be due to overstocking and/or improper water management. To investigate the problem, experiments were performed in commercial storage tanks stocked with oysters at different densities. The aim of this study was to provide some basic experimental data and guidelines for better handling of oysters prior to shipping
Tipo: Text Palavras-chave: Physiology; Oyster farmers; Crassostrea gigas; Oysters; Storage tanks; Physiologie; Ostréiculture; Dégorgeoirs; Crassostrea gigas; Huîtres; Bassin stockage.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3047.pdf
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Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph ArchiMer
Hubert, Florence; Van Der Knaap, Wil; Noel, Thierry; Roch, Philippe.
Mussel (Mytilus galloprovincialis) plasma contains cytotoxic activity against both vertebrate (erythrocytes and mouse tumour) and protozoan cells. Procaryotes (Escherichia coli and Vibrio alginolyticus) were not sensitive to the cytotoxicity. The activity was still present in dialyzed samples but was inhibited by heating at 45°C. Large individual variability which was not correlated with protein concentration and an increasing number of reactive specimens following injection, suggested that naive mussels were in various stages of immune response. Purification by anion exchange chromatography followed by gel filtration revealed a 320 kDa cytotoxic polymeric protein that acts through a polymerization process after binding onto target cell membranes as...
Tipo: Text Palavras-chave: Cytotoxicité; Antibactérien; Hémolymphe; Lysat d'hémocyte; Moule; Huître; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas; Cytotoxicity; Antibacterial; Hemolymph; Hemocyte lysate; Musssel; Oyster; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00039/15008/12333.pdf
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Essais de propagation in vitro de virus de type herpès infectant les mollusques bivalves marins et contribution à l'étude des interactions hôte-virus ArchiMer
Deniau, Stephane.
Des infections à virus de type herpès sont rapportées chez différentes espèces de mollusques bivalves, à travers le monde. Elles sont associées à de fortes mortalités de larves et de juvéniles, pendant les périodes chaudes de l'année. Ce type d'infection virale a été observé sous forme de cellules anormales au moyen de la microscopie photonique et de particules enveloppées à conformation icosaédrique, en microscopie électronique à transmission (MET). Aujourd'hui, il est possible de diagnostiquer la présence du virus grâce à des outils moléculaires tels que la polymérisation en chaîne (PCR) ou l'hybridation in situ (HIS). Cependant, l'absence de lignées cellulaires homologues représente une difficulté majeure. En effet, la recherche d'effet cytopathogène et...
Tipo: Text Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Infection; Herpèsvirus; Culture cellulaire; Propagation; Mécanisme de défense; Apoptose; Récepteur au TNF; Cytométrie de flux.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00025/13631/10714.pdf
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Anomalies de croissance de la coquille de Crassostrea gigas dans le bassin de Marennes -Oléron. Bilan de toris années d'observation ArchiMer
Heral, Maurice; Berthome, Jean-paul; Polanco Torres, E.; Alzieu, Claude; Deslous-paoli, Jean-marc; Razet, Daniel; Garnier, Jacqueline.
The appearance and changes of the oyster shell's gelatinous bags have been monitored since 1979. The increase in thickness that causes the growth into a "ball" can be explained by an overproduction of gel. The various environmental factors that can intervene in the secretion of gel are successively examined.
Tipo: Text Palavras-chave: Oysters; Shell; Crassostrea gigas; Huîtres; Coquille; Crassostrea gigas.
Ano: 1981 URL: http://archimer.ifremer.fr/doc/1981/acte-2943.pdf
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Etude de la croissance et de la qualité de trois lots d'huître creuse Crassostrea gigas dans le bassin de Marennes-Oléron en 1979 ArchiMer
Berthome, Jean-paul; Fernandez-castro, Nora.
Three oyster samplers, collected in 1976, 77 and 78 have beer grown, each one after 3 differents densities in order to determine the influence of the load of breeding over growth and improvement of quality of oysters. The results show that quality of oysters is rapidly altered when the density of breeding increases and that gametogenesis physiolical prevails over growth, particularly for older oyster. A variation of the date of "détroquage" (oysters off supports that collect spat) will be determined, according to spat collecting and speed of spat growth. We are here suggesting a "production"coefficient.
Tipo: Text Palavras-chave: Marennes Oléron; Naissain; Croissance; Crassostrea gigas; Huîtres.
Ano: 1980 URL: http://archimer.ifremer.fr/doc/1980/acte-3002.pdf
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Exposure to the toxic dinoflagellate Alexandrium catenella modulates juvenile oyster Crassostrea gigas hemocyte variables subjected to different biotic conditions ArchiMer
Lassudrie, Malwenn; Soudant, Philippe; Nicolas, Jean-louis; Miner, Philippe; Le Grand, Jacqueline; Lambert, Christophe; Le Goic, Nelly; Hegaret, Helene; Fabioux, Caroline.
The Pacific oyster Crassostrea gigas is an important commercial species cultured throughout the world. Oyster production practices often include transfers of animals into new environments that can be stressful, especially at young ages. This study was undertaken to determine if a toxic Alexandrium bloom, occurring repeatedly in French oyster beds, could modulate juvenile oyster cellular immune responses (i.e. hemocyte variables). We simulated planting on commercial beds by conducting a cohabitation exposure of juvenile, “specific pathogen-free” (SPF) oysters (naïve from the environment) with previously field-exposed oysters to induce interactions with new microorganisms. Indeed, toxic Alexandrium spp. exposures have been reported to modulate bivalve...
Tipo: Text Palavras-chave: Crassostrea gigas; Alexandrium; Harmful algal blooms; Hemocyte; Microbial challenge.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00314/42523/41894.pdf
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Croissance de l'huitre japonaise Crassostrea gigas en cylindre rotatif Stanway. Premiers resultats en France ArchiMer
Robert, Rene; Maurer, Daniele.
A new method of oyster cultivation, the Stanway oyster cylinder, has been experimented in the basin of Arcachon since the autumn of 1989. The first observations on three species of juvenile bivalves showed that this system could be successfully used with the Japanese oyster Crassostrea gigas . A comparative study of the growth of this last species, reared in cylinders and oyster bags, has been carried out since spring 1990. The first results showed that the growth of juveniles and 18 months old oysters in cylinders is not as good as in bags. On the other hand, mortality did not differ between both techniques: less than 3%. This new system seems to be less efficient than the traditional French way of rearing oysters. However, an improvement in the quality...
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Juveniles; Oyster culture; Growth; Rearing.
Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/acte-917.pdf
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Contrôle des performances biologiques de populations diploïdes et triploïdes d'huîtres creuses Crassostrea gigas ArchiMer
Benhaim, David.
Un programme concerannt le contrôle des perforùances biologiques de lots diploïdes et triploïdes d'huîtres creuses Crassostrea gigas a débuté en mars 1993 à La Tremblade et dans d'autres station IFREMER associées. Le présent rapport rend compte des cinq premiers mois d'analyses faisant partie d'un contrôle qui devrait se poursuivre jusqu'en 1994. A La Tremblade, l'expéreince repose principalement sur la comparaison des paramètres biométriques et biochimiques des lots diploïdes et triploïdes en deux sites d'étude : un parc ostréicole sur le bassin de Marennes-Oléron et une claire. Les individus diploïdes réfractaires du lot triploïde, sont identifiés par imagerie numérique et écartés des analyses. Les résultats obtenus montrent une difféence marquée entre...
Tipo: Text Palavras-chave: Crassostrea gigas; Triploïdes; Diploïde; Estran; Croissance; Biochimie.
Ano: 1993 URL: http://archimer.ifremer.fr/doc/00411/52220/52948.pdf
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Activité comparée chez les espèces Crassostrea angulata Lmk et Crassostrea gigas Thunberg, en hiver ArchiMer
His, Edouard.
L'espèce Crassostrea gigas a été introduite en 1969 à titre d'essai par de nombreux ostréiculteurs du bassin d'Arcachon. La comparaison des vitesses de croissance de cette nouvelle espèce et de l'huître indigène C. angulata est nettement à l'avantage de l'huître japonaise. Cette dernière atteint la taille marchande dans la plupart des cas dès la première année d''immersion dans la baie alors qu'il faut généralement de deux à trois ans pour obtenir le même résultat avec l'huître portugaise.
Tipo: Text Palavras-chave: Ostréiculture; Huître; Crassostrea angulata; Crassostrea gigas; Physiologie; Comparaison; Bassin Arcachon; Gironde.
Ano: 1971 URL: http://archimer.ifremer.fr/doc/00009/11979/8683.pdf
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L'élevage de l'huître creuse à Marennes-Oléron en 1987 : Estimation des stocks cultivés ArchiMer
Bodoy, Alain; Geairon, Philippe.
Le bassin de Marennes-Oléron est le premier centre ostréicole de France, avec un stock de 93 700 tonnes en août 1987. L'opération d'échantillonnage a montré que ce chiffre comprenait 36 500 tonnes pour l'élevage à plat, 33 000 tonnes pour la première phase d'élevage sur tables, qui concerne l'élevage sur collecteurs de captage et 24 200 tonnes pour la phase finale représentée par l'élevage en poche. Le niveau du stock est en augmentation constante depuis 1984. L'importance pondérale de chaque classe d'âge est susceptible de fluctuer très largement puisque des huîtres âgées de 1 an représentaient 4 400 tonnes en 1987, alors que la classe d'âge correspondante représentait 17 000 tonnes en 1986. Ceci est dû aux variations annuelles du captage. Une méthode...
Tipo: Text Palavras-chave: Ostréiculture; Stocks; Captage; Croissance; Production; Huîtres creuses; Crassostrea gigas; Marennes Oléron; Charente Maritime.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00014/12480/9326.pdf
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BABE : Comparaison des sites de Ronce et Baie de Veys, incidence sur la reproduction et les mortalités : Ecologie côtière, mortalités, croissance, environnement - Physiologie - Immunologie ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Guilpain, Patrice; Geairon, Philippe; Razet, Daniel; Seugnet, Jean-luc; Robert, Stephane; Ropert, Michel; Simonne, Charlotte; Hugonnet, Virginie; Moal, Jeanne; Pouvreau, Stephane; Huvet, Arnaud; Samain, Jean-francois; Lambert, Christophe; Soudant, Philippe; Frouel, Stephane; Choquet, Gwenaelle; Delaporte, Maryse; Paillard, Christine.
Four laboratories are involved in this multidisciplinary study: the Conchological Laboratory of Normandie (LCN), the Invertebrates Physiology Laboratory (LPI), the Université Bretagne Occidentale (LEMAR IHP) and the Conchological Laboratory of Poitou-Charentes (LCPC). The part of the study which is presented here (thematics "Coastal ecology") was produced in collaboration with the LCN. The data on the Bay of Veys was collected by Michel Ropert. This study aims at observing the "sites" effect on mortality and on the general biology of the animal, as well as measuring some environmental elements (e.g. water temperature, etc.). The geographical sites are Gefosse in the Bay of Veys (East Cotentin) and the Ronce bank in the south of the Marennes Oléron Bay. The...
Tipo: Text Palavras-chave: Immunologie; Physiologie; Paramètres environnementaux; Croissance; Ecologie côtière; Baies des Veys Cotentin; Ronce Charente Maritime; Comparaison sites; Reproduction; Mortalités; Crassostrea gigas; Huîtres.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/acte-3405.pdf
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Estimation des stocks sauvages d'huîtres creuses dans les pertuis charentais en 2010 ArchiMer
Soletchnik, Patrick; Geairon, Philippe; Le Moine, Olivier; Robert, Serge.
Une évaluation des stocks d’huîtres sauvages est effectuée en 2010 au cours d’une campagne visant à mesurer le risque de contamination des huîtres sauvages par les huîtres d’élevage et inversement, dans le contexte de l’épidémie à herpes virus qui touche l’ostréiculture depuis 2008. L’investigation conduite sur 21 secteurs des pertuis charentais, incluant les cotes Ouest des îles de Ré et d’Oléron, font apparaître une très forte hétérogénéité de répartition des stocks d’huîtres sauvages, fortement concentrées à l’Est des pertuis Breton et d’Antioche. L’estimation faite à partir des 21 secteurs est d’environ 35000 tonnes d’huîtres sauvages ; stock probablement sous estimé car certaines zones rocheuses modestes n’ont pas été étudiées. Quoiqu’il en soit, la...
Tipo: Text Palavras-chave: Stocks sauvages; Stocks naturels; Huître creuse; Crassostrea gigas; Bassin de Marennes.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00084/19485/17104.pdf
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Enhancing resistance to Vibrio aestuarianus in Crassostrea gigas by selection ArchiMer
Dégremont, Lionel; Azéma, Patrick; Maurouard, Elise; Travers, Marie-agnes.
Ostreid herpesvirus 1 (OsHV-1) and Vibrio aestuarianus are the two main pathogens affecting the production of French oyster (Crassostrea gigas). The absence of genetic correlation between the two diseases is promising for the development of stocks with dual resistance. Using unselected and selected oysters concerning enhanced resistance to OsHV-1 infection, we investigated the first generation of mass selection and the response to selection to increase the resistance to V. aestuarianus for two stocks. For each stock, four groups were produced in June 2013 using either parents unchallenged with the bacteria or counterparts that survived experimental infections by the bacteria. Thus, groups were unselected oysters for both pathogens, selected for either the...
Tipo: Text Palavras-chave: Crassostrea gigas; Realized heritability; Vibrio aestuarianus; OsHV-1; Disease resistance.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00628/74033/73362.pdf
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Influence of OSHV-1 oyster mortality episode on dissolved inorganic fluxes: An ex situ experiment at the individual scale ArchiMer
Richard, Marion; Bourreau, Juliette; Montagnani, Caroline; Ouisse, Vincent; Le Gall, Patrik; Fortune, Martine; Munaron, Dominique; Messiaen, Gregory; Callier, Myriam; Roque D'Orbcastel, Emmanuelle.
Ostreid herpesvirus 1 (OsHV-1 μvar) infection has caused significant mortalities in juvenile oysters (Crassostrea gigas). In contrast to the practices of other animal production industries, sick and dead oysters are not separated from live ones and are left to decay in the surrounding environment, with unknown consequences on fluxes of dissolved materials. A laboratory approach was used in this study to test the influence of oyster mortality episode on dissolved inorganic fluxes at the oyster interface, dissociating (i) the effect of viral infection on metabolism of juvenile oysters and (ii) the effect of flesh decomposition on oxygen consumption and nutrient releases at the individual scale. Nine batches of juvenile oysters (Individual Total wet weight...
Tipo: Text Palavras-chave: Crassostrea gigas; Mortality; Ostreid herpesvirus 1; Juvenile; Spat; Mineralisation; Oxygen consumption; Nutrient fluxes.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00378/48973/49380.pdf
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Experimental ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: Kinetics of virus DNA detection by q-PCR in seawater and in oyster samples ArchiMer
Schikorski, David; Faury, Nicole; Pepin, Jean-francois; Saulnier, Denis; Tourbiez, Delphine; Renault, Tristan.
Herpes- and herpes-like viruses are known to infect a wide range of bivalve mollusc species throughout the world. Abnormal summer mortalities associated to the detection of ostreid herpesvirus 1 (OsHV-1) have been currently reported in France among larvae and spat of the Pacific cupped oyster Crassostrea gigas. In the present work, we have developed an experimental protocol of horizontal transmission based on the cohabitation between healthy and experimentally infected oysters. Through a cohabitation trial, the kinetics of OsHV-1 detection in different oyster organs and seawater samples were investigated and characterized for the first time using real time quantitative PCR.
Tipo: Text Palavras-chave: Ostreid herpesvirus 1; Cupped Pacific oyster; Crassostrea gigas; Experimental infection; Kinetic viral DNA quantification.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00025/13607/10688.pdf
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REMONOR : Résultats 2003. Evaluation de la mortalité, croissance et qualité des huîtres creuses ArchiMer
Simonne, Charlotte; Pien, S.; Hugonnet, Virginie; Le Gagneur, Eric; Ropert, Michel; Kopp, Joel; Richard, O..
The network named REMONOR (REseau MOllusqueNORmandie) aims to assess mortality, growth and tissue quality of the Pacific oyster, Crassostrea gigas, cultivated in Normandy over an annual cycle. This local network relies on the same material and methods as the national one named REMORA (REseau Mollusques Ressources Aquacoles). The study was carried out in 17 sites, which were distributed in the four basins where oyster rearing occurs in Normandy (West and East coast of Cotentin, Baie des Veys and Meuvaines). In 2003, a new site was added, which was located in the area of Ver-Sur-Mer (Calvados). The results obtained in 2003 showed a low annual mortality for adults (8%) as well as for juveniles (10%). Growth was also low for the two age classes of oyster...
Tipo: Text Palavras-chave: REMORA; Quality; Growth; Mortality; Normandy; REMONOR; Crassostrea gigas; REMORA; Qualité; Croissance; Mortalité; Basse Normandie; REMONOR; Crassostrea gigas.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/rapport-2246.pdf
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