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Potencial fisiológico de sementes de mamona tratadas com micronutrientes - doi: 10.4025/actasciagron.v32i4.4829 Agronomy
Oliveira, Rosa Honorato de; Universidade FederalRural dePernambuco, Unidade Acadêmica de Serra Talhada; Souza, Myrne Jamilly de Lima; UESB; Morais, Otoniel Magalhães; UESB; Guimarães, Bruno Vinicius Castro; UESB; Pereira Júnior, Hercílio de Assis; UESB.
O objetivo deste trabalho foi verificar os efeitos da aplicação de micronutrientes sobre a germinação e o vigor de sementes de mamona, especificamente a cultivar BRS 149 Nordestina. Utilizou-se o delineamento experimental inteiramente casualizado, com quatro repetições de 25 sementes. Os tratamentos constaram da testemunha, imersão em H2O, imersão em B, imersão em Zn, imersão em Cu, imersão em Mn, imersão em Mo e imersão em Fe. Por meio dos resultados obtidos, evidencia-se incremento na germinação e no vigor com a aplicação de micronutrientes às sementes de mamona. O B influenciou negativamente a germinação e o vigor e aumentou a porcentagem de sementes duras e dormentes.
Palavras-chave: 5.01.00.00-9 e 5.01.03.00-8 Ricinnus communis L.; Germinação; Sementes; Vigor; Plântulas; Nutrientes Fertilidade do Solo e Adubação Ricinus communis L.; Germination; Seeds; Vigor; Seedlings; Nutrients.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/4829
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Potential and restrictions of Poincianella pyramidalis (Tul.) L. P. Queiroz as native forage in the Brazilian semi-arid region Animal Sciences
França, Andrezza Araújo de; Silva, Divan Soares da; Fechine, Josean Tavares; Sousa, Francinilda Alves de; Andrade, Alberício Pereira de; Lichston, Juliana Espada.
Poincianella pyramidalis (catingueira) is a endemic plant of the Caatinga, selected by animals grazing on native pasture. With the aim of evaluating characteristics indicative of its nutritional quality, 10 plants were selected and identified, sampled at five different ages, were used to determine dry matter (DM), crude protein (CP), neutral detergent fibre (NDF), mineral matter (MM), DM degradability (Deg DM), NDF degradability (Deg NDF) and in situ and in vitro leaf-tissue degradability. Phytochemical prospection was performed, and 1H and 13C nuclear magnetic resonance applied to detect the presence of secondary compounds. The data were submitted to analysis of variance and Tukey’s test at 5%, and correlation analysis was carried out on the variables for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Degradability; Native forage; Nutrients; Plant defence; Tannins..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/47460
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Dynamics of nitrogen concentration on intercropped ryegrass - 10.4025/actascianimsci.v34i1.12661 Animal Sciences
Skonieski, Fernando Reimann; Universidade Federal de Santa Maria (UFSM); Viégas, Julio; Universidade Federal de Santa Maria (UFSM); Cruz, Pablo; Institut National de la Recherche Agronomique (INRA); Nornberg, José Laerte; Universidade Federal de Santa Maria (UFSM); Bermudes, Rogério Fôlha; Universidade Federal de Santa Maria (UFSM); Gabbi, Alexandre Mossate; Universidade Federal do Rio Grande do Sul (UFRGS).
The dynamics of nitrogen (N) in ryegrass intercropped with different forage species with regard to dry matter (DM) accumulation within an agroecological transition system was evaluated. Annual ryegrass was intercropped with black oats (RY + BO), white clover (RY + WC) and forage peanut (RY + FP). The experimental design was completely randomized, with three replications. The study applied the N dilution model for plant tissue which comparatively uses modifiable factors of the environment at optimal levels. The model shows decline in N concentration due to the DM accumulation of temperate grass pastures. Data were compared to model by the root mean square deviation analysis. Dilution coefficient (β), used in all pasture samples, was lower than -0.60....
Palavras-chave: Dilution coefficient; Forage peanut; Legumes; Nutrients; Oat; White clover.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/12661
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Limnology of a lateral lagoon system connected to a Neotropical reservoir (Rosana reservoir, São Paulo/Paraná, Brazil) Biological Sciences
Ferrareze, Mateus; Instituto de Biociências; Nogueira, Marcos Gomes; Instituto de Biociências; Sartori, Luciana; Instituto de Biociências.
The aim of this work was to perform a comparative analysis of four lateral lagoons and of the main channel of the Rosana Reservoir (Paranapanema river), southeast Brazil. The fieldwork was conducted during dry and rainy periods of 2004 and 2005. The analyzed variables were chlorophyll a, turbidity, total phosphorus, total nitrogen, dissolved nutrients (ammonium, nitrate, nitrite, phosphate and silicate), Secchi disk transparency, suspended solids, temperature, pH, dissolved oxygen and electrical conductivity. Intense summer rainfall provided a high input of allochthonous material into the system, resulting in conspicuous changes - high turbidity and nutrient concentrations and low transparency, especially in the reservoir channel. The cluster analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ecologia Limnologia floodplain; Nutrients; Paranapanema river; Limnological variables; Spatial variability; Eutrophication Ecologia.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/21491
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Effects of intensive fish-farming and domestic wastewater on the periphytic algal community in a tropical coastal lagoon (Juara, Brazil) - doi: 10.4025/actascibiolsci.v35i3.17094 Biological Sciences
Almeida, Stéfano Zorzal de; Instituto de Botânica; Fernandes, Valéria de Oliveira; Universidade Federal do Espírito Santo.
This study aimed at evaluating the effects of the intensive fish-farming and the domestic wastewater on the structure of the phycoperiphyton community. Three experimental supports containing artificial substrate were assembled in two sampling sites: IF – region with intensive fish-farming and DW – near the domestic wastewater discharge. Samplings were carried out after the 21st, 26th and 31st day of colonization. The abiotic variables evaluated were: transparency, electrical conductivity, pH, turbidity, total suspended solids, alkalinity, dissolved oxygen, water temperature and nutrients. Phycoperiphyton was examined with regard to density, richness, abundance, dominance, diversity and eveness. Nutrients (total nitrogen, nitrate, ammonia nitrogen,...
Tipo: Estudos exploratórios Palavras-chave: 2.05.00.00-9; Bacillariophyceae; Late successional stage; Nutrients; Phycoperiphyton; Artificial substrate 2.05.00.00-9.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17094
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Periphytic diatom as bioindicators in urban and rural streams - doi: 10.4025/actascibiolsci.v36i1.18175 Biological Sciences
Moresco, Carina; Universidade Estadual de Maringá; Rodrigues, Liliana; Universidade Estadual de Maringá.
Periphytic diatoms were sampled from two streams, one located in an urban area and the other located in a rural area, in Maringá, State of Paraná, Brazil. In addition to temporal variability, differences in the assemblage structure between streams were evaluated and correlated with physical and chemical characteristics. Six samples of periphytic diatoms were collected from each stream from July 2007 to June 2008. The streams differed in abiotic factors and in relation to the structure of diatom assemblages and the spatial scale was more important than the temporal scale (ANOVA, p ≥ 0.05; non-metric multidimensional scaling (NMS) stress = 13.73, p = 0.009). The Procrustes analysis (m2 = 0.7607 and p = 0.0001) showed that variables with the greatest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ciências Biológicas - Ecologia indicator species; Nutrients; Human impact; Bacillariophyta; Periphyton Diatomáceas Perifíticas.
Ano: 2013 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/18175
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Influence of a net cage tilapia culture on the water quality of the Nova Avanhandava reservoir, São Paulo State, Brazil - doi: 10.4025/actascibiolsci.v34i3.7298 Biological Sciences
Mallasen, Margarete; Instituto de Pesca; Barros, Helenice Pereira de; Instituto de Pesca; Traficante, Daniela Polizeli; Estadual Paulista “Julio de Mesquita Filho”; Camargo, André Luiz Scarano; Escama Forte Aquacultura e Meio Ambiente Ltda.
In order to understand the influence of a net cage tilapia culture on the environment, water quality parameters were investigated during the period between December, 2005 and November, 2007. Three sampling stations were established in the reservoir of Nova Avanhandava (Zacarias, São Paulo State) as follows: upstream of net cage area (P1), in the rearing place (P2) and downstream of net cage area (P3). The mean values of the parameters examined in the water sampling stations were within the standards of water quality recommended by resolution no. 357/2005 of the Conselho Nacional do Meio Ambiente for class 2 freshwater bodies. A significantly higher mean concentration of total phosphorus (p < 0.05) in the P2 (0.035 mg L-1) was the result of the...
Tipo: Estudo da qualidade de água Palavras-chave: 5.06.02.01-2 limnology; Phosphorus; Nutrients; Intensive fish farming; Tilapia Fatores abióticos de águas interiores.
Ano: 2012 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/7298
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Characterization of Dairy Slurry in Sourthern Chile Farms Agricultura Técnica
Salazar,Francisco; Dumont,Juan Carlos; Chadwick,David; Saldaña,Rodolfo; Santana,Mabel.
In this study 50 slurry samples were collected from commercial dairy farms located in the South of Chile during 1995-1997. Samples were analyzed for dry matter, pH, organic matter, available and total macro nutrients (nitrogen, phosphorus, potassium, calcium, magnesium and sodium), available and total micro nutrients (zinc, iron, manganese and copper) and total aluminum. Results showed that the dry matter content in dairy effluents was low, with 62% of the samples being < 4% DM. This reflected the large volumes of water that the storage received. A great variation in nutrient content was observed in the analyzed samples, probably reflecting differences in feeding regimes, use of wash water, rainfall and age of slurry at the time of sampling. In general,...
Tipo: Journal article Palavras-chave: Nutrients; Dairy effluents; Cattle slurry; Farm waste.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072007000200005
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Limnology of the largest multi-use artificial reservoir in NE Brazil: The Castanhão Reservoir, Ceará State Anais da ABC (AABC)
LACERDA,LUIZ D.; SANTOS,JANAÍNA A.; MARINS,ROZANE V.; SILVA,FRANCISCO A.T.F. DA.
Abstract This work reviews the limnology of the largest multi-use reservoir in NE Brazil, the Castanhão Reservoir in Ceará State, during 5 years of an extended drought when the reservoir’s volume decreased from 88% to about 30%. Major physical and chemical parameters of the water column, phytoplankton community, trophic state and sediment geochemistry were monitored, as well as the impact from extensive aquaculture. Water quality of the full reservoir was maintained due to hydrodynamics, which transport nutrients to the hypolimnion of a stratified water column, rendering an oligotrophic state to the reservoir, notwithstanding the large nutrient inputs from aquaculture and irrigated agriculture. However, with the extension of the drought period, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrochemistry; Hydrodynamics; Nutrients; Water quality; Sediments; Aquaculture.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502073
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Spatial changes in the water quality of Itajaí-Açú Fluvial-Estuarine System, Santa Catarina, Brazil Anais da ABC (AABC)
Pereira-Filho,Jurandir; Rörig,Leonardo R.; Schettini,Carlos A.F.; Soppa,Mariana A.; Santana,Bruno L.; Santos,José Eduardo dos.
This study was carried out with the aim of evaluating the spatial variation of the water quality in the Itajaí-Açú River estuary. Seven stations along the estuary were monitored on a weekly basis, from October 2003 to December 2004, plus two stations in tributaries (Itajaí-Mirim River, the main tributary, and one reference station). This monitoring included measurements of salinity, pH, dissolved oxygen, temperature, nutrients(NH+4,NO3-2,NO-3,PO3-4,H4SiO4) Biochemical Oxygen Demand (BOD), total phosphorous and dissolved organic phosphorus (TP and DOP), particulate organic carbon (POC), suspended particulate matter (SPM) and chlorophyll-a (Cla). Multivariate analyses demonstrated the compartmentalization of the system based on the deterioration in water...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Itajaí Estuary; Nutrients; Organic matter; River; Santa Catarina.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400019
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Timing and placement of cattle manure and/or gliricidia affects cotton and sunflower nutrient accumulation and biomass productivity Anais da ABC (AABC)
PRIMO,DÁRIO C.; MENEZES,RÔMULO S.C.; OLIVEIRA,FABIO F. DE; DUBEUX JÚNIOR,JOSÉ CARLOS B.; SAMPAIO,EVERARDO V.S.B..
ABSTRACT Organic fertilizers are a viable alternative to increase oilseed productivity in family agriculture systems. The study aimed to evaluate the effects of timing and placement of cattle manure and/or gliricidia (Gliricidia sepium Jacq. Walp) prunings on cotton (Gossipium hirsutum L.) and sunflower (Helianthus annuus L.) nutrient accumulation and biomass productivity. Experiments were carried out in 2010 and 2011 in Taperoá, Paraíba, Brazil. The organic fertilization treatments were: GI - gliricidia incorporated before planting; GS - gliricidia applied on surface 45 days after planting (DAP); MI + GI - manure and gliricidia incorporated before planting; MI + GS - manure incorporated before planting and gliricídia applied on the surface 45 DAP; MI -...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Helianthus annuus L.; Gossypium hirsutum L.; Green manure; Nutrients; Productivity..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100415
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Phosphorus dynamics in a highly polluted urban drainage channel-shallow reservoir system in the Brazilian semiarid Anais da ABC (AABC)
ARAÚJO,GERMÁRIO M.; LIMA NETO,IRAN E.; BECKER,HELENA.
Abstract: This paper investigates phosphorus dynamics in a highly polluted system composed of a drainage channel followed by a shallow reservoir in the city of Fortaleza, Brazilian semiarid. During the dry season, significant non-point source loads of total phosphorus originated from residences unconnected to sewers were identified along the channel, resulting in an increasing flow rate of untreated sewage towards the reservoir. During the rainy season, as a consequence of dilution, phosphorus concentrations decreased by about 5-fold. The results also revealed that the reservoir was acting similarly to a waste stabilization pond, with phosphorus removal efficiency of about 33%. Assuming one-dimensional flow for the channel and complete mixing for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diffuse pollution; Eutrophication; Nutrients; Semiarid; Urbanization; Water quality modeling.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500902
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The use of rocks to improve family agriculture in Brazil Anais da ABC (AABC)
Theodoro,Suzi H.; Leonardos,Othon H..
During the second half of the 20th century, the introduction of new chemical agriculture technologies brought about a revolution in food production, but this has also cause deep deterioration in our relationships with the natural environment. In developing tropical countries, record yields were accompanied by rural exodus, widespread deforestation and loss of topsoil. The stonemeal research described herein is an alternative to such practices as it is an environmental friendly and socially responsible technology based on soil rejuvenation and conservation by means of the addition of natural rock powders that are rich in phosphorous, calcium, magnesium, potassium and micronutrients. The research adopted an inter-disciplinary approach and was conducted among...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stonemeal; Nutrients; Small farmers.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000400008
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Compositional studies and biological activities of some mash bean (Vigna mungo (L.) Hepper) cultivars commonly consumed in Pakistan Biol. Res.
Zia-Ul-Haq,Muhammad; Ahmad,Shakeel; Bukhari,Shazia Anwer; Amarowicz,Ryszard; Ercisli,Sezai; Jaafar,Hawa ZE.
BACKGROUND: In recent years, the desire to adopt a healthy diet has drawn attention to legume seeds and food products derived from them. Mash bean is an important legume crop used in Pakistan however a systematic mapping of the chemical composition of mash bean seeds is lacking. Therefore seeds of four mash bean (Vigna mungo (L.) Hepper, family Leguminoseae) cultivars (NARC-Mash-1, NARC-Mash-2, NARC-Mash-3, NARC-Mash-97) commonly consumed in Pakistan have been analyzed for their chemical composition, antioxidant potential and biological activities like inhibition of formation of advanced glycation end products (AGE) activity and tyrosinase inhibition activity. RESULTS: The investigated cultivars varied in terms of biochemical composition to various...
Tipo: Journal article Palavras-chave: Nutrients; Antioxidant potential; Mash bean cultivar; Pakistan.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100023
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Effect of the inherent variation in the mineral concentration of alfalfa cultivars on aphid populations Bragantia
Silva,Alexandre de Almeida e; Varanda,Elenice Mouro; Primavesi,Ana Cândida.
Plants have inherent variability of mineral content which affects their physiology and consequently the herbivorous insects feeding on them. Besides, insects need considerable amounts of potassium, phosphorus and magnesium in their diets, whereas little calcium, sodium and chloride are required. In this study, the inherent variation on mineral (Ca, S, Mg, N, P, K and also C:N ratio) concentrations and aphid (Acyrthosiphon spp., Therioaphis maculata, Aphis craccivora) populations on three alfalfa (Medicago sativa) cultivars (P3; Crioula, the most widely cultivated in Brazil, and CUF 101, an aphid-resistant) were studied between September/1997 and August/1998. A significant variation on mineral concentrations and aphid populations was observed among...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aphididae; Insecta; Nutrients; Resistance; Plant-insect relation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052005000200010
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Food quantity and quality of cassava affected by leguminous residues and inorganic nitrogen application in a soil of low natural fertility of the humid tropics Bragantia
Moura-Silva,Aline Gomes; Aguiar,Alana das Chagas Ferreira; Jorge,Neuza; Agostini-Costa,Tânia da Silveira; Moura,Emanoel Gomes.
ABSTRACT The aim of this study was to test the hypothesis that the quality and quantity of biofortified cassava root in a humid tropical environment can be modified with the application of a combination of low- and high-quality residues of leguminous tree species. The experiment was designed as a 6 × 2 factorial (a combination of 4 legume species versus 2 levels of nitrogen) with 4 replications in a randomized block design and the following treatments: Gliricidia + Acacia, Gliricidia + Leucaena, Gliricidia + Clitoria, Leucaena + Acacia, Leucaena + Clitoria, and a control without legumes. We analyzed the shoot weight, number of roots/plant, root weight, root production, proximate composition, as well as the mineral, carotenoid, and pro-vitamin A contents....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofortification; Food security; Green manure; Nutrients.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000300406
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Carbon Dioxide Biofixation and Production of Spirulina sp. LEB 18 Biomass with Different Concentrations of NaNO3 and NaCl BABT
Moraes,Luiza; Rosa,Gabriel Martins da; Souza,Michele da Rosa Andrade Zimmermann de; Costa,Jorge Alberto Vieira.
ABSTRACT Microalgae are efficient at using solar energy to turn CO2 and nutrients into biomass containing lipids, proteins, carbohydrates and other compounds that may be used to produce bioproducts for human and animal consumption and pharmaceutical use. The aim of this study was to assess the effect of the NaNO3 and NaCl concentration on the growth kinetics, the biomass composition and the ability to biofix CO2 using the microalga Spirulina sp. LEB 18. The assays were carried out according to a 22 central composite design (CCD) with different concentrations of NaNO3 (1.25, 1.88 and 2.50 g L-1) and NaCl (1.00, 15.0 and 30.0 g L-1). The assays were carried out in 2 L vertical tubular photobioreactors at 30°C, 12 h light/dark and an injection of 12.0% v/v of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CO2; Fixation; Growth kinetics; Microalgae; Nutrients.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100410
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Changes in nutrient loads (N, P and Si) in the São Francisco estuary after the construction of dams BABT
Medeiros,Paulo Ricardo Petter; Knoppers,Bastiaan Adriaan; Cavalcante,Geórgenes Hilário; Souza,Weber Friederichs Landim de.
The aim of this study was to investigate the post-dam conditions of the loads and yields of dissolved inorganic nitrogen (DIN), orthophosphate (DIP, silicate (DSi) and total phosphorous (TP) in the Lower São Francisco river estuary (NE-Brazil) after the river was regulated to a constant flow by the dams. Loads and yields of monthly measurements performed from November 2000 to March 2002 at a gauging station downstream of the dams (80 km from the coast) showed 4.1 x 10³ t/yr and 0.006 t/km² /yr of DIN, 0.2 x 10³ t/yr and 0.002 t/km² /yr of DIP, and 448 x 10³ t/yr and 0.71 t/km² /yr of DSi, respectively. Over the last 15 years, DIN loads reduced by 94 % and DSi by 31%. The river turned into an oligotrophic system with primary production limited by nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nutrients; Fluvial loads; Dam impacts; Estuary; São Francisco River (NE-Brazil).
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200022
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Methanogenesis in an impacted and two dystrophic coastal lagoons (Macaé, Brazil) BABT
Furtado,André Luiz dos Santos; Casper,Peter; Esteves,Francisco de Assis.
This study investigated the methanogenic activity in sediment of the Imboacica (human impacted), Cabiúnas and Comprida coastal lagoons in Rio de Janeiro State (Brazil). Methane was not detected in water and sediment samples from the three lagoons. The measured nutrient concentrations in the pore-water indicated that methanogens activity was not limited by nutrients. Methanogenic activity was not detected under in vitro conditions, indicating that terminal organic carbon mineralisation via methanogenesis was negligible for the top 6 cm of sediment at the sampling time.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coastal lagoon; Methane; Sediment; Nutrients.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000200011
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Seasonal variability of coastal water quality in bay of Bengal and Palk Strait, Tamilnadu, Southeast Coast of India BABT
Srinivasan,Viswanathan; Natesan,Usha; Parthasarathy,Anitha.
The aim of this work was to study the physicochemical parameters of water quality collected from 12 sampling stations from Topputhurai to Muthupet in Vedaranyam located on the southeast coast of India from January to December 2008. Results showed that the DO and nutrients were the maximum in the Bay of Bengal during the monsoon period. High concentration of the nutrients in summer season was obtained near the Muthupet mangroves compared to the Palk Strait, which showed that this acted as a source of nutrients to the adjacent coastal waters. Low concentrations of the nutrients observed in the monsoon could be attributed to the terrestrial runoff from Muthupet lagoon. The physicochemical characteristics of coastal waters between the Point Calimere and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coastal zone; Physico-chemistry of water; Nutrients; Vedaranyam; Bay of Bengal; Palk Strait.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500020
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