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Registros recuperados: 233 | |
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Pedroso,Reginaldo dos Santos; Ferreira,Joseane Cristina; Costa,Karen Regina Carim da; Candido,Regina Celia. |
Various organisms have been characterized by molecular methods, including fungi of the genus Cryptococcus. The purposes of this study were: to determine the discriminatory potential of the RAPD (Random Amplified Polymorphic DNA) primers, the pattern of similarity of the Cryptococcus species, and discuss their useful application in epidemiological studies. We analyzed 10 isolates of each specie/group: C. albidus, C. laurentii complex, C. neoformans var. grubii, all from environmental source, and two ATCC strains, C. neoformans var. grubii ATCC 90112, and C. neoformans var. neoformans ATCC 28957 by RAPD-PCR using the primers CAV1, CAV2, ZAP19, ZAP20, OPB11 and SEQ6. The primers showed a good discriminatory power, revealing important differences between them... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptococcus albidus; Cryptococcus laurentii; RAPD; Molecular Markers. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300015 |
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Almeida,Margarete Teresa Gottardo; Rubio,Fernando Gôngora; Garcia,Doroti Oliveira; Pavarino-Bertelli,Érika Cristina; Rossit,Andrea Regina Baptista; Bando,Silvia Yano; Silbert,Suzane; Goloni-Bertollo,Eny Maria; Soares,Márcia Maria Costa Nunes; Martinez,Marina Baquerizo. |
Stenotrophomonas maltophilia is a Gram-negative bacillus, which is becoming widely recognized as an important nosocomial pathogen. The main objective of this study was to evaluate the genetic relatedness, by random amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of 86 clinical isolates of S. maltophilia (colonization 22, infection 64) obtained from 79 hospitalized patients, from different geographic regions of São Paulo State. The genotypic analysis performed by RAPD and PFGE was used in 24 isolates for genetic identity confirmation. The results were congruent between the two methods but it was not possible to link genetic profiles with the studied variables, clinical state and geographic area, probably due to the great... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Stenotrophomonas maltophilia; RAPD; PFGE. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200017 |
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Maki,Cristina Sayuri; Teixeira,Flavia França; Paiva,Edilson; Paccola-Meirelles,Luzia Doretto. |
The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37ºC) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28ºC. The water content was lower in strains grown at 37ºC. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Edible mushroom; RAPD; Mycelial growth. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300002 |
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Silva,Rosana Bellan de Oliveira; Fusco-Almeida,Ana Marisa; Matsumoto,Marcelo Teruyuki; Baeza,Lilian Cristiane; Benaducci,Tatiane; Mendes-Giannini,Maria José Soares. |
Trichosporon asahii is an opportunistic pathogen, associated with a high mortality rate in immunocompromised patients. In this study, ten isolates, recovered from oral cavity and urine of patients in Intensive Care Units (ICU) over six months, were identified by classical and molecular methods, typed by RAPD and tested in vitro for susceptibility to fluconazole, itraconazole, 5-flucytosine and amphotericin B. A total agreement between the identification of Trichosporon sp by PCR based on sequences of the Internal Transcribed Spacer Regions (ITS) and on the sequences of small-subunit (SSU) ribosomal DNA (rDNA) was found. Randomly amplified of polymorphic DNA (RAPD), with primers P6 and M13, was used to determine the genomic profiles. The dendogram analysis... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichosporon; PCR; RAPD; Susceptibility; Antifungal drugs. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300033 |
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Mori,Edson S; Martins,Dagoberto; Velini,Edivaldo D; Marino,Celso L; Gouvêa,Cantídio F; Leite,Suzi M.M; Camacho,Edwin; Guries,Raymond P. |
The aquatic plant species Egeria densa (Planch.) Casp and E. najas Planchon occur naturally in the Paraná River Basin of southeastern Brazil. Hydroelectric dam construction in the river basin has created several lakes, changing the ecology of the river and altering the population ecology of Egeria. Large, dense populations of Egeria now grow in Jupiá Lake and its tributaries, occasionally blocking hydroelectric turbines. This study is part of a larger project examining environmental changes associated with large dam projects; the research objective of this study was to assess patterns of genetic diversity in Egeria populations growing in Jupiá and Três Irmãos Lakes and their Paraná River tributaries using genetic markers. Forty-two plants of E. najas and... |
Tipo: Journal article |
Palavras-chave: Egeria; Dam; Isoenzyme; Genetic distance; RAPD; Reservoir. |
Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200008 |
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Povh,Jayme A; Lopera Barrero,Nelson M; Ribeiro,Ricardo P; Lupchinski Jr.,Enio; Gomes,Patricia C; Lopes,Tais S. |
Las poblaciones de peces nativos están disminuyendo debido a varios factores que impactan en el ambiente. Como forma de abordar este problema, se han realizado varias acciones como por, ejemplo, el repoblamiento. Sin embargo, estas acciones se realizan sin ningún respaldo científico. Esto puede, además de no ser eficiente, provocar una pérdida de la diversidad genética en las poblaciones, pudiendo ocasionar impactos irreversibles en las próximas generaciones. Por esta razón, el empleo de marcadores moleculares, que permitan monitorear genéticamente las poblaciones parece fundamental para lograr la conservación de la ictiofauna |
Tipo: Journal article |
Palavras-chave: Conservación genética; Ictiofauna; Microsatélite; Pez; RAPD; Variabilidad genética. |
Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202008000100001 |
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Lopera-Barrero,Nelson M; Povh,Jayme A; Ribeiro,Ricardo P; Gomes,Patricia C; Jacometo,Carolina B; Silva Lopes,Tais da. |
El uso de apropiados métodos de muestreo, tipo de tejido y la utilización de protocolos viables de extracción del ADN son aspectos críticos en estudios basados en PCR. Los métodos de extracción de ADN deben ser simples, reproducibles, económicos y no contaminantes. Este trabajo tuvo por objetivo comparar un protocolo modificado de extracción con sal común (NaCl) y un protocolo modificado de extracción con fenol-cloroformo para la obtención de ADN genómico en alta cantidad y calidad desde muestras de aleta y larva de pez (Brycon orbignyanus, Piaractus mesopotamicus, Oreochromis niloticus, Leporinus elongatus y Prochilodus lineatus). Las muestras aisladas de diferentes especies de peces usando la extracción con sal común permitieron obtener ADN de buena... |
Tipo: Journal article |
Palavras-chave: Amplificación por PCR; Estudios de peces; Investigación genética; Microsatélites; RAPD. |
Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202008000100008 |
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Busso,Cleverson; Kaneshima,Edilson Nobuyoshi; Franco,Francisco de Assis; Querol,Carmen Boto; Castro-Prado,Marialba Avezum Alves de. |
Fusarium graminearum isolates causing Fusarium head blight in wheat were collected in Brazil and analyzed by random amplified polymorphic DNA (RAPD) markers and vegetative compatibility grouping (VCG). Nitrate non-utilizing mutants (nit) from each isolate were paired to verify heterokaryon formation. Three VCGs were identified among F. graminearum isolates: VCG1 included F-2, F-3 and F-4 isolates; VCG2 included F-1, F-6 and F-9 isolates; VCG3 included F-5, F-7 and F-8 isolates. Based on PCR amplification with eight different primers, the isolates showed great genetic similarity among themselves. Dendrogram analysis demonstrated two RAPD groups: Group A, consisting of isolates F-2 and F-9, and Group B, composed of the remaining isolates. Results suggest the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fusarium graminearum; Genetic variability; RAPD; Vegetative compatibility groups. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000600050 |
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Chitarra,Cristiane Silva; Silva,Mayara Inácio Vincenzi da; Brandão,Laila Natasha Santos; Kagueyama,Francielle Cristina; Candido,Stefhano Luis; Rosa,Janaina Marcela Assunção; Nakazato,Luciano; Dutra,Valéria. |
ABSTRACT: Swine respiratory diseases such as atrophic rhinitis and bronchopneumonia caused by Pasteurella (P.) multocida cause important economic losses to the modern swine industry. The purpose of this study was to characterize P. multocida strains isolated from swine lungs by RAPD (Randomly Amplified Polymorphic DNA) to demonstrate their genetic diversity. Ninety-four samples of fragments from lungs with pneumonia and sixty one samples without pneumonia were collected in slaughterhouses in Mato Grosso during the period from December 2009 to March 2010. Clinical cases in 2012 and 2013 were also included in this study. Among the lung fragments with macroscopic lesions, without macroscopic lesions and clinical samples, 40.42%, 4.49% and 100% were positive... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulmonary pasteurellosis; Swine; Pneumonia; Pasteurella multocida; RAPD. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100119 |
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Silva,Cristiane Maria da; Hinz,Robert Harri; Stadnik,Marciel João; Pereira,Adriana; Tcacenco,Fernando Adami. |
O mal-do-panamá, causado por Fusarium oxysporum f. sp. cubense (FOC), é um dos principais problemas fitossanitários da bananicultura. O uso de cultivares resistentes é o método preferencialmente recomendado para o seu controle, sendo a avaliação da diversidade genética do patógeno necessária no desenvolvimento de estratégias de manejo da doença a longo prazo. Assim, este trabalho teve como objetivo estudar a variabilidade genética de isolados de FOC por marcadores moleculares RAPD e SSR. Foram avaliados 64 isolados coletados em regiões produtoras do estado de Santa Catarina, sendo que 100% deles foram patogênicos à bananeira da cv. 'Enxerto'. As análises de conglomerados com esses marcadores revelaram variabilidade entre os isolados amostrados. As técnicas... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Banana; Doença; Fungo; RAPD; SSR. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001200007 |
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Carvalho,Márcio Fonseca de; Crestani,Maraisa; Farias,Francine Lunardi; Coimbra,Jefferson Luís Meirelles; Bogo,Amauri; Guidolin,Altamir Frederico. |
O conhecimento da diversidade genética, por meio da dissimilaridade entre os genótipos, permite a organização, a amostragem e a utilização eficiente do germoplasma em programas de melhoramento genético. O objetivo deste trabalho foi estimar a dissimilaridade entre acessos crioulos de feijão coletados no Estado de Santa Catarina, integrantes do banco ativo de germoplasma de feijão da UDESC e de três cultivares comerciais ("Pérola", "SCS 202-Guará" e "BRS Valente"), por meio de marcadores moleculares RAPD. Foram utilizados 21 iniciadores decâmeros que permitiram a visualização de 96 bandas, sendo que 41 (42,7%) apresentaram polimorfismo entre os acessos estudados, resultando em bandas entre 650 e 2000pb. A dissimilaridade foi calculada utilizando-se o... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris L.; Germoplasma; RAPD; Dissimilaridade. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600005 |
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Silva,Ana Veruska Cruz da; Martins,Antonio Baldo Geraldo. |
A ausência de sementes tem sido uma característica bastante exigida pelos consumidores de uvas de mesa. O objetivo deste trabalho foi identificar marcas moleculares associadas à ausência de sementes, utilizando as técnicas RAPD e fAFLP. Foram utilizadas folhas jovens de 19 cultivares. Na análise RAPD 30, iniciadores possibilitaram amplificação de todas as amostras, produzindo 392 bandas polimórficas. Foi possível encontrar uma marca específica para a ausência de sementes, utilizando o iniciador UBC 443, que poderá futuramente ser utilizado para o desenvolvimento de marcadores SCAR, possibilitando a criação de um teste de identificação rápida e precoce de apirenia em videira. A análise fAFLP proporcionou a visualização de um dendrograma com grupos... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vitis; RAPD; FAFLP; Apirenia. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000300012 |
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Registros recuperados: 233 | |
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