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| Cezar,Alfredo Skrebsky; Glanzner,Werner Giehl; Gutierrez,Karina; Anezi-Junior,Paulo Afonso; Nilles,Carlise Reichert; Comim,Fábio Vasconcellos; Vogel,Fernanda Silveira Flores; Gonçalves,Paulo Bayard Dias. |
| Cell therapy has shown encouraging perspectives for human and veterinary medicine. Experimentally, genetic manipulation allows to mark and locate allogeneic cells. However, this makes their genotype/phenotype different from non-marked cells used clinically. Alternatively, the presence of the Y-chromosome enables male donor cells detection in female organisms. However, the concentration of engrafted cells may be minimal in tissues, due to systemic distribution. In this study, a nested-PCR multiplex test was developed, aiming to increase the sensitivity of the presence/absence diagnosis of male mice adipose-derived (ADSC-Y) and bone marrow mononuclear (BMNC-Y) cells in samples of blood and lungs from females, after endovenous transplantation. Four females... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cellular therapy; Transplantation; Allogeneic cells; Diagnosis; Y-chromosome.. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500905 |
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| Campos,Monique Paiva de; Luca,Paula Mello De; Renzetti,Alinne Rangel dos Santos; Souza,Sara Maria Marques de; Mendes Júnior,Artur Augusto Velho; Barros,Renata Simões; Figueiredo,Fabiano Borges. |
| ABSTRACT: The objective of the current research was to assess seroconversion in dogs immunized with Leishmune® and Leish Tec® vaccines using rapid chromatographic immunoassay DPP® (Dual Path Platform) (DPP CVL) and enzyme immunoassay (EIE) up to one year after the vaccination protocol. The study sample comprised 28 dogs divided into two groups, each group immunized with an anti-CVL vaccine and clinically monitored for one year through clinical evaluation and laboratory tests. 22 (78.5%) dog were monitored. During the evaluation time (T1-30 days, T2-6 months, and T3-1 year after vaccination) the results for all dogs were negative for CVL, except for one animal vaccinated with Leish tec® that seroconverted in the DPP CVL test at T2. Subsequent examinations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine visceral leishmaniasis; Vaccine; Diagnosis; Seroconversion. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000400503 |
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| Makino,Herica; Sousa,Valéria Régia Franco; Fujimori,Mahyumi; Rodrigues,Juliana Yuki; Dias,Alvaro Felipe Lima Ruy; Dutra,Valéria; Nakazato,Luciano; Almeida,Arleana do Bom Parto Ferreira de. |
| ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine ehrlichiosis; Diagnosis; Nested PCR; Biological samples.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310 |
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| Kurissio,Jacqueline Kazue; Rodrigues,Marianna Vaz; Taniwaki,Sueli Akemi; Zanutto,Marcelo de Souza; Filoni,Claudia; Galdino,Maicon Vinícius; Araújo Júnior,João Pessoa. |
| ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1) may causes an asymptomatic infection that result in an efficient transmission and subsequently dissemination of the virus in feline population. This study used molecular detection by qPCR (quantitative PCR) based on DNA polymerase gene fragment amplification to evaluate the occurrence of FcaGHV1 and its correlation with other feline viral pathogens, such as Carnivore protoparvovirus 1 (CPPV-1), Felid alphaherpesvirus 1 (FeHV-1), and feline retroviruses such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Of the 182 blood samples evaluated 23.6% (43/182) were positives for FcaGHV1. Approximately 37.9% (33/87) of the samples that tested positive for retrovirus were also were positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gammaherpesvirus; Herpesvirus; Feline pathogen; Infectious diseases; Diagnosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300451 |
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| Fantinatti,Alexandra Pinheiro; Daleck,Carlos Roberto; Nunes,Newton; Alessi,Antonio Carlos; Costa Neto,João Moreira da; Vicenti,Felipe Antonio Mendes; Duque,Juan Carlos; Santos,Paulo Sérgio Patto dos. |
| Hepatic biopsy was realized through laparoscopy with simultaneous cauterization in the present study, whose principal aim was to evaluate the efficacy of the applied method and to study its effects in the liver of healthy dogs. Furthermore, we tried to verify the main hematological and chemistry profile alterations related to the hepatic function, and to investigate the viability of the fragments collected by histopathology. To attain this objective, 21 clinically healthy dogs, weighing between 10 and 15kg were submitted to hepatic biopsy with forceps connected to the cautery. Cautery was performed by applying radiofrequency energy at 45 watts. Forty-two hepatic biopsies through laparoscopy were conducted in the animals. At group I one hepatic fragment per... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electrocautery; Dog; Surgery; Diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000400019 |
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| Moreira,Simone Magela; Machado,Rosângela; Passos,Lygia Friche. |
| The present work describes the detection of infected cells in the bone marrow aspirates of dogs experimentally infected with a Brazilian isolate of Ehrlichia canis. Dogs were monitored twice a day by clinical evaluation and peripheral blood smear examination. Every three days, blood samples were collected for cell counts. Weekly, aspirates from the bone marrow were examined and serum samples were tested by IFAT. The clinical signs observed were fever, pallid membranes, lymphadenopathy, serous nasal secretions, and pronounced weight loss. Hematological alterations included normocytic normochromic anemia, decrease of neutrophils and lymphocytes, and thrombocytopenia. Few E. canis infected cells were seen in blood smears. However, stages of E. canis were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ehrlichia canis; Diagnosis; Bone marrow puncture; Dogs. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000400038 |
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| Registros recuperados: 230 | |
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