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| Registros recuperados: 4.682 | |
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| Goldfarb,Míriam; Santana,Mateus Ferreira; Salomão,Tânia Maria Fernandes; Queiroz,Marisa Vieira de; Barros,Everaldo Gonçalves de. |
| Abstract Two retrotransposons from the superfamilies Copia and Gypsy named as Copia-LTR_SS and Gypsy-LTR_SS, respectively, were identified in the genomic bank of Sclerotinia sclerotiorum. These transposable elements (TEs) contained direct and preserved long terminal repeats (LTR). Domains related to codified regions for gag protein, integrase, reverse transcriptase and RNAse H were identified in Copia-LTR_SS, whereas in Gypsy-LTR_SS only domains for gag, reverse transcriptase and RNAse H were found. The abundance of identified LTR-Solo suggested possible genetic recombination events in the S. sclerotiorum genome. Furthermore, alignment of the sequences for LTR elements from each superfamily suggested the presence of a RIP (repeat-induced point mutation)... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Phytopathogens; Retrotransposons; Transposable elements. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300426 |
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| Mauro,Antonio Orlando; Oliveira,Antonio Luiz de; Mauro,Sonia Marli Z.. |
| A study was made of the genetics of resistance to the soybean cyst nematode, race 3, in a population derived from crosses between the Brazilian soybean genotypes BR 90-4722 and FT-Cristalina. Crosses between the two parents were made, the F1 and F2 generations were obtained and the population was analyzed for the number of cysts found in each plant of each generation as well as the type of reaction to the nematode. The results showed that resistance to the cyst nematode in this soybean population is of a qualitative nature and conditioned by three genes, one dominant and two recessives. The heritability of the character was very high (0.96), with minimum environmental effect, which means that this population is suitable for the development of soybean... |
| Tipo: Info:eu-repo/semantics/other |
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| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571999000200021 |
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| Javier Tovar,Francisco; Silva,Luiz Antônio Ferreira da; Rodarte,Renato Santos; Leoncini,Orilio. |
| Ribosomal DNA (rDNA) is a multigenic family composed of one or more clusters of repeating units (RU). Each unit consists of highly conserved sequences codifying 18S, 5.8S and 28S rRNA genes intercalated with poorly conserved regulatory sequences between species. In this work, we analyzed the rDNA of Drosophila arizonae, a member of the mulleri complex (Repleta group). Using genomic restriction patterns, cloning and mapping of some representative rDNA fragments, we were able to construct a representative restriction map. RU in this species are 13.5-14 kb long, restriction sites are completely conserved compared with other drosophilids and the rDNA has an R1 retrotransposable element in some RU. We were unable to detect R2 elements in this species. |
| Tipo: Info:eu-repo/semantics/other |
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| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200015 |
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| Grimaldi,Rogério; Acosta,Angelina Xavier; Cabral-Oliveira,Fernando Cézar; Brites,Carlos; Galvão-Castro,Bernardo. |
| The main coreceptor gene involved in HIV-1 infection is CCR5 beta chemokine receptor gene for which several mutations have been described, some of which have correlated with HIV-1 infection, acquired immune deficiency syndrome (AIDS), or both. Deletion of 32bp in the CCR5 gene (delta32) has been shown to confer resistance to infection by HIV-1 R5 strains. Another mutation, characterized by a thymine to adenine (T to A) nucleotide substitution at position 303 (m303), has shown the same effects as the delta32 mutation, with previous studies having shown that the allele frequency of the CCR5-m303 mutation is 0.014 in African-American and 0.007 in French populations. The Brazilian population is known to be genetically diverse, because of which we investigated... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: CCR5 gene; M303 frequency; Brazilian populations; HIV-1. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200006 |
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| Bruschi,Daniel Pacheco; Sousa,Deborah Yasmim; Soares,Amanda; Carvalho,Klélia Aparecida de; Busin,Carmen Sílvia; Ficanha,Natália Cristine; Lima,Albertina Pimentel; Andrade,Gilda Vasconcellos; Recco-Pimentel,Shirlei Maria. |
| Abstract The genus Rhinella is one of the most diverse groups of bufonid toads, currently composed by 93 valid species and naturally distributed throughout different Neotropical ecoregions. Here, we analyze nine Brazilian populations of toads representing species of the Rhinella margaritifera and Rhinella marina groups. These new data include the first description of the R. hoogmoedi and R. proboscidae karyotypes, as well as other taxonomically unresolved forms. Chromosomal analysis of the populations revealed pronounced chromosomal uniformity (2n=22), including the diploid number and chromosomal morphology. Three different NOR-bearing chromosomes were identified: in the subterminal region of pair 10q in R. hoogmoedi, Rhinella sp. 1 and Rhinella sp. 2, in... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Bufonid toads; Nucleolar organizing region; Chromosomal evolution. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300445 |
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| Carvalho,Flávia Maria de Souza; Caramori,Lázara Pereira Campos; Leite Júnior,Rui Pereira. |
| Xanthomonas axonopodis pv. citri strains that cause disease in citrus were investigated by pulsed field and plasmid profile analysis. For the first method, genomic DNA was digested by the rare-cutting enzymes Xba I and Vsp I. The strains evaluated were collected in seven different States of Brazil and in Argentina, Bolivia, Paraguay and Uruguay. Genetic variability was found among strains of X. axonopodis pv. citri from different geographical areas Argentina, Bolivia and Uruguay, with similarities varying from 0.62 to 0.83. However, the strains collected in Brazil, despite being from different States, have shown a genetic similarity ranging from 0.83 to 1.00. Cluster analysis showed a relationship between genomic similarity and geographical origin of the... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Genetic variability; Bacterium; Citrus; PFGE. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000300020 |
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| Bellagamba,Bruno Corrêa; Abreu,Bianca Regina Ribas de; Grivicich,Ivana; Markarian,Carolina Franke; Chem,Eduardo; Camassola,Melissa; Nardi,Nance Beyer; Dihl,Rafael Rodrigues. |
| Abstract Mesenchymal stem cells (MSCs) are known for their important properties involving multilineage differentiation potential., trophic factor secretion and localization along various organs and tissues. On the dark side, MSCs play a distinguished role in tumor microenvironments by differentiating into tumor-associated fibroblasts or supporting tumor growth via distinct mechanisms. Cisplatin (CIS) is a drug widely applied in the treatment of a large number of cancers and is known for its cytotoxic and genotoxic effects, both in vitro and in vivo. Here we assessed the effects of CIS on MSCs and the ovarian cancer cell line OVCAR-3, by MTT and comet assays. Our results demonstrated the resistance of MSCs to cell death and DNA damage induction by CIS,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Mesenchymal stem cells; Cisplatin; MTT; Comet assay; OVCAR-3 cells. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100129 |
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| Registros recuperados: 4.682 | |
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