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Registros recuperados: 273
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Mitochondrial and nuclear DNA phylogeography of two cupped oysters Crassostrea gigas and Crassostrea angulata ArchiMer
Boudry, Pierre; Huvet, Arnaud.
The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data...
Tipo: Text Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf
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Genetic improvement and selection in shellfish : a review based on oyster research and production ArchiMer
Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfill market demand. Up to now, the most effective answer to disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability of suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability of the shellfish industry. The different approaches to genetic improvement include...
Tipo: Text Palavras-chave: Selective breeding; Hybridization; Polyplidy; Introduction; Oysters; Selection; Hybridation; Polyploidie; Introduction; Huitres.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3499.pdf
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Cytogenetic study of Ostrea conchaphila (Mollusca : Bivalvia) and comparative karyological analysis within Ostreinae ArchiMer
Leitao, Alexandra; Chaves, Raquel; Santos, Sara; Boudry, Pierre; Guedes Pinto, Henrique; Thiriot Quievreux, Catherine.
Chromosome preparations of the Olympia oyster Ostrea conchaphila Carpenter were studied using conventional Giemsa, silver staining, and C-banding techniques. The karyotype consists of six metacentric (1, 2, 4, 6, 8, and 10) and four submetacentric (3, 5, 7, 9) chromosome pairs. The silver-stained nucleolus organizer regions (Ag-NORs) were terminally located on the short arms of the submetacentric pair 5 (56% of cases) and on the long arms of submetacentric pair 7 (6% of cases). Constitutive heterochromatin was observed as telomeric C-bands on the short arm of the NOR-bearing chromosome pair 5 and as centromeric blocks of several chromosome pairs. Comparative analysis of patterns of karyotype, Ag-NORs, and C-bands of this species and of five other flat...
Tipo: Text Palavras-chave: Ostreinae; Cytotaxonomy; Cytogenetics; Ostrea conchaphila.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-2792.pdf
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Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions ArchiMer
Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf
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Pacific cupped oyster - Crassostrea gigas ArchiMer
Lapegue, Sylvie; Boudry, Pierre; Goulletquer, Philippe.
Originating from the north eastern Asia, Crassostrea gigas is endemic to Japan, but has been introduced and translocated, mainly for aquaculture purpose, into several countries, almost worldwide (1). In North America, the species can be found from Southeast Alaska to Baja California, while in European waters the species is cultured from Norway to Portugal as well as in Mediterranean Sea (Fig.1) (2). Biological characteristics make it suitable for a wide range of environmental conditions, although it is usually found in coastal and estuarine areas within its natural range. Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (3).
Tipo: Text Palavras-chave: Genetics; Ecology; Crassostrea gigas; Biology; Oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3322.pdf
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Impact of intensive larval rearing practices in oyster hatcheries : selective processes and loss of genetic variability (slides) ArchiMer
Taris, Nicolas; Sauvage, Christopher; Boudry, Pierre.
Oysters, like many marine bivalves, are characterized by very high fecundity and low survival rate at early stages of life. Rearing conditions in hatcheries (i.e. density, feeding, temperature...) are different from those observed in the wild. Additionally, slow growing larvae are culled by sieving. As previous studies have shown that genetic variability exists for several early development traits, we aimed to assess if common hatchery rearing practices exert specific selective processes at the larval stage. In order to avoid possible bias and to increase the number of families studied, we used a mixed-family approach with subsequent parentage analysis using a recently developed set of three multiplexed microsatellite markers. We studied the variance in...
Tipo: Text Palavras-chave: Hatcheries; Selection; Genetic drift; Genetic; Crassotrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3483.pdf
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Genetic improvement and selection in shellfish : a review based on oyster research and production ArchiMer
Boudry, Pierre; Barre, Marc; Gerard, Andre.
Despite the economic importance of shellfish, genetic improvement has not yet had a great impact on these species. Shellfish farming is traditionally based on wild species whose natural populations are often overexploited and do not fulfil market demand. Up to now, the most effective answer 10 disease problems or for the improvement of productivity has been the introduction of new species. However, the introduction of new species is constrained by their ecological impact and is also limited by the availability 01 suitable species. Genetic improvement of local species should therefore be of great importance as a viable alternative for the long term sustainability 01 the shellfish Industry. The different approaches 10 genetic improvement include...
Tipo: Text Palavras-chave: Selective breeding; Hybridization; Polyploidy; Introduction; Oyter; Sélection; Hybridation; Polyploïdie; Introduction; Huîtres.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2740.pdf
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Genetic effect of domestication selective pressures on Pacific oyster at larval stage ArchiMer
Taris, Nicolas; Sauvage, Christopher; Ernande, Bruno; Boudry, Pierre.
Among bivalve species, the Pacific oyster, Crassostrea gigas, is the most economically important bivalve production over the world. Today, C. gigas is subject to an important production effort that leads to an intensive artificial selection. Larval stage is relatively unknown, specifically in a domestication context. Genetic consequence of artificial selection is still at a preliminary study. We aimed to tackle the consequence of inconscient domestication on the variance reproductive success focusing on larval stage, keystone of the life cycle. We studied two kinds of specific selective processes that common hatchery rearing practices exert : the effect of discarding the smallest larvae on genetic diversity and the artificial environment rearing effect via...
Tipo: Text Palavras-chave: Selection; Genetic correlations; Genetic variability; Genetic; Domestication selective; Crassostrea gigas; Pacific oysters.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3441.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridations et conservatoire de souches. Contrat de Plan Etat Région Poitou- Charentes 1994-1998 - Convention 95/RPC-R-57 "Génétique" - Rapport année 1995 ArchiMer
Boudry, Pierre; Naciri, Yamama; Heurtebise, Serge; Delsert, Claude; Ledu, Christophe; Phelipot, Pascal; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous les membres du Réseau Génétique Mollusques (REGEMO), a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou-Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses.
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea virgnica.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15515/12902.pdf
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European flat oyster - Ostrea edulis ArchiMer
Lapegue, Sylvie; Beaumont, A.; Boudry, Pierre; Goulletquer, Philippe.
The European flat oyster, Ostrea edulis, a native of Europe, occurs naturally from Norway to Morocco in the North-Eastern Atlantic and in the whole Mediterranean Basin; It has been a harvested species for at least 6000 years. Natural populations are also observed in eastern North America, from Maine to Rhode Island, following intentional introductions in the 1940s and 1950s. The species was also introduced in Canada for aquaculture purpose 30 years ago and some populations naturalised in Nova Scotia, New Brunswick and British Columbia. These stocks were imported from naturalised populations in Maine whose ancestors originated in the Netherlands.
Tipo: Text Palavras-chave: Genetics; Ecology; Biology; Ostrea edulis; Oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3321.pdf
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Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). I. Estimation of genetic parameters for survival and growth ArchiMer
Degremont, Lionel; Ernande, Bruno; Bedier, Edouard; Boudry, Pierre.
The multidisciplinary project "MOREST" aims to improve our understanding of causes of summer mortality in Crassostrea gigas juveniles in France and to reduce its impact on oyster production. As pail of the MOREST project, 43 full-sib families nested within 17 half-sib families were produced, planted out and tested in 3 sites during summer 2001 to assess to what extent genetic variability exists for this trait. A strong genetic basis was found for survival as narrow-sense heritability estimates ranged from 0.47 to 1.08, with higher values in sites where summer mortality was higher. Genetic correlations across sites were positive and very high for survival, indicating no genotype by environment interaction. In contrast, lower genetic variation was observed...
Tipo: Text Palavras-chave: Growth; Survival; Juveniles; Heritability; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2457.pdf
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Research and development for the genetic improvement of the Pacific oyster in France : present and prospects ArchiMer
Boudry, Pierre; Haffray, Pierrick.
The most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) in France to date has been through the breeding of triploids, especially since the development of tetraploids. Triploid oysters commonly resist summer mortality better than diploids. This is most probably because reproductive allocation is so important in this phenomenon. Quantitative genetics studies strongly suggest that a significant gain in resistance to summer mortality, or other traits of interest, could be obtained by selective breeding. Practical difficulties however, and the high cost of breeding large numbers of families under common conditions over many generations remain major constraints to family-based selective breeding in oysters. In the last...
Tipo: Text Palavras-chave: Polyploidization; Selective breeding; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3438.pdf
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Genetic parameters of resistance to Vibrio aestuarianus, and OsHV-1 infections in the Pacific oyster, Crassostrea gigas, at three different life stages ArchiMer
Azema, Patrick; Lamy, Jean-baptiste; Boudry, Pierre; Renault, Tristan; Travers, Marie-agnes; Degremont, Lionel.
Background In France, two main diseases threaten Pacific oyster production. Since 2008, Crassostrea gigas spat have suffered massive losses due to the ostreid herpesvirus OsHV-1, and since 2012, significant mortalities in commercial-size adults have been related to infection by the bacterium Vibrio aestuarianus. The genetic basis for resistance to V. aestuarianus and OsHV-1 and the nature of the genetic correlation between these two traits were investigated by using 20 half-sib sire families, each containing two full-sib families. For each disease, controlled infectious challenges were conducted using naïve oysters that were 3 to 26 months old. In addition, siblings were tested under field, pond and raceway conditions to determine whether laboratory...
Tipo: Text
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00372/48350/48518.pdf
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Review and practical use of genome mapping in aquacultured organisms. Position paper by the Working Group on the Application of Genetics in Fisheries and Mariculture (WGAGFM) ArchiMer
Boudry, Pierre; Lapegue, Sylvie; Guyomard, R..
The Working Group on the Application of Genetics in Fisheries and Mariculture (WGAGFM) met in La Rochelle, France from 10-12 March, 2003. Seven persons representing six countries were present. Three terms of reference (ToR) were addressed, while one was deferred until the appropriate information was available for review. WGAGFM first considered ToR a) Review and report on the practical use of genome mapping in aquacultured organisms. Genome maps are used extensively in the production of selected strains of agriculture plants and livestock. Studies on the genome of aquatic animais have been summarised in the WG report. Genome mapping projects in aquaculture exist for most major species and linkage maps are now becoming available. WGAGFM produced four...
Tipo: Text Palavras-chave: Conservation of genetic diversity; Triploids; Recommandation; Aquaculture; Fish; Genome mapping; Genetic.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3301.pdf
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Scanning the genome of the Pacific cupped oyster, Crassostrea gigas, for adaptative differentiation during geographic range expansion ArchiMer
Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie.
Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci...
Tipo: Text Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf
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Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment ArchiMer
Morvezen, Romain; Cornette, Florence; Charrier, Gregory; Guinand, Bruno; Lapegue, Sylvie; Boudry, Pierre; Laroche, Jean.
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Tipo: Text Palavras-chave: Microsatellites; Polymerase chain reaction; Parentage; Assignment; Bivalve mollusc.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00137/24822/22907.pdf
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Diversité et différentiation génétique chez l'huître plate européenne : de la population à l'individu (Actes) ArchiMer
Boudry, Pierre; Launey, Sophie; Diaz-almela, A.; Naciri-graven, Yamama; Ledu, Christophe; Mira, S.; Taris, Nicolas; Bonhomme, Francois; Lapegue, Sylvie.
PCR-based DNA markers enable the study of diversity and differentiation of marine organisms at different stages of their life cycle. Our study of European fiat oyster (Ostrea edulis) populations aimed to describe their spatial and temporal dynamics. First, we analyzed adults sampled over the natural range of the species. An Atlantic/Mediterranean differentiation pattern was observed. Similar patterns of variation were found using allozymes, microsatellites and mitochondrial DNA, aIl which support an isolation by distance model. High variability was observed in the range of diversity between populations using a mitochondrial marker, a reflection of small effective population sizes in sorne locations. A 10fold quantitative difference was observed in Fst,...
Tipo: Text Palavras-chave: Recruitment; Diversity; Molecular markers; Population genetics; Ostrea edulis; Flat oyster; Dynamique de recrutement; Diversité génétique; Microsatellites; Marqueurs moléculaires; Génétique des populations; Ostrea edulis; Huître plate.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-2851.pdf
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Detailed insights into pan-European population structure and inbreeding in wild and hatchery Pacific oysters (Crassostrea gigas) revealed by genome-wide SNP data ArchiMer
Vendrami, David L. J.; Houston, Ross D.; Gharbi, Karim; Telesca, Luca; Gutierrez, Alejandro P.; Gurney-smith, Helen; Hasegawa, Natsuki; Boudry, Pierre; Hoffman, Joseph I..
Cultivated bivalves are important not only because of their economic value, but also due to their impacts on natural ecosystems. The Pacific oyster (Crassostrea gigas) is the world's most heavily cultivated shellfish species and has been introduced to all continents except Antarctica for aquaculture. We therefore used a medium-density single nucleotide polymorphism (SNP) array to investigate the genetic structure of this species in Europe, where it was introduced during the 1960s and has since become a prolific invader of coastal ecosystems across the continent. We analyzed 21,499 polymorphic SNPs in 232 individuals from 23 localities spanning a latitudinal cline from Portugal to Norway and including the source populations of Japan and Canada. We confirmed...
Tipo: Text Palavras-chave: Aquaculture; Crassostrea gigas; Genetic structure; High-density genotyping array; Inbreeding; Pacific oyster; Restriction site-associated DNA (RAD) sequencing; Single nucleotide polymorphism (SNP).
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00484/59561/62557.pdf
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NORA moving forward: Developing an oyster restoration network in Europe to support the Berlin Oyster Recommendation ArchiMer
Pogoda, Bernadette; Boudry, Pierre; Bromley, Cass; Cameron, Tom C.; Colsoul, Bérenger; Donnan, David; Hancock, Boze; Hugh‐jones, Tristan; Preston, Joanne; Sanderson, William G.; Sas, Hein; Brown, Janet; Bonacic, Kruno; Nordheim, Henning; Zu Ermgassen, Philine S.e..
The Native Oyster Restoration Alliance (NORA) supports the protection and ecological restoration of the native European oyster, Ostrea edulis, and its habitat across its current and historical biogeographical range. NORA works to overcome barriers to the conservation, restoration, and recovery of the European oyster by providing a platform for the NORA community to collaborate and participate in knowledge exchange. NORA seeks to support responsible restoration practice, in compliance with biosecurity and sustainability. Against this background, the NORA community formulated a series of specific recommendations, the Berlin Oyster Recommendation, to support native oyster restoration by developing and applying best practice with the aim to recover healthy and...
Tipo: Text Palavras-chave: Ecological restoration; Native Oyster Restoration Alliance; Ostrea edulis.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00659/77135/78451.pdf
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Etude du niveau d'aneuploïdie dans les populations des zones de captage du bassin de Marennes-Oléron - Contrat Plan Etat Région Poitou-Charentes 1999 - Rapport 1999 ArchiMer
Lapegue, Sylvie; Thiriot Quievreux, Catherine; Mccombie, Helen; Heurtebise, Serge; Boudry, Pierre; Robert, Stephane; Soletchnik, Patrick; Goulletquer, Philippe; Gerard, Andre.
Divers travaux, menés depuis 1984, montrent qu'il existe, chez l'huître creuse Crassotrea gigas, des cellules montrant un nombre anormal de chromosomes (2n = 19, 18 ou même 17 au lieu de 2n = 20). Le niveau d'aneuploïdie est déterminé par le décompte des chromosomes à partir de suspensions cellulaires de tissu branchial. Le pourcentage de cellules aneuploïdes est toujours significativement supérieur dans les "lots de queue", c'est-à-dire les huîtres présentant des croissances plus faibles, et peut atteindre plus de 30 %. Récemment, une étude réalisée au sein du programme européen « Genephys » a démontré que plus de 50 % de la variance pour la vitesse de croissance était liée au taux d'aneuploïdie. Le but de l'expérimentation menée en 1999 est de faire un...
Tipo: Text Palavras-chave: Génétique; Aneuploïdie; Génétique des populations; Biochimie; Marennes-Oléron; Huître creuse; Crassostrea gigas.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00044/15522/12909.pdf
Registros recuperados: 273
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