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Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean Genet. Mol. Biol.
Pereira,Wendell Jacinto; Melo,Arthur Tavares de Oliveira; Coelho,Alexandre Siqueira Guedes; Rodrigues,Fabiana Aparecida; Mamidi,Sujan; Alencar,Sérgio Amorim de; Lanna,Anna Cristina; Valdisser,Paula Arielle Mendes Ribeiro; Brondani,Claudio; Nascimento-Júnior,Ivanildo Ramalho do; Borba,Tereza Cristina de Oliveira; Vianello,Rosana Pereira.
Abstract Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Water deficit; Functional genomics; Single nucleotide polymorphism (SNP).
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100309
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DNA replication stress: oncogenes in the spotlight Genet. Mol. Biol.
Primo,Luiza M. F.; Teixeira,Leonardo K..
Abstract Precise replication of genetic material is essential to maintain genome stability. DNA replication is a tightly regulated process that ensues faithful copies of DNA molecules to daughter cells during each cell cycle. Perturbation of DNA replication may compromise the transmission of genetic information, leading to DNA damage, mutations, and chromosomal rearrangements. DNA replication stress, also referred to as DNA replicative stress, is defined as the slowing or stalling of replication fork progression during DNA synthesis as a result of different insults. Oncogene activation, one hallmark of cancer, is able to disturb numerous cellular processes, including DNA replication. In fact, extensive work has indicated that oncogene-induced replication...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cancer; Cell cycle; DNA replication; Oncogene; Replication stress.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200301
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Genome sequence of Shewanella corallii strain A687 isolated from pufferfish (Sphoeroides spengleri) Genet. Mol. Biol.
Azevedo,Gustavo P.R.; Paz,Pedro H. C da; Mattsson,Hannah K.; Moreira,Ana Paula B.; Leomil,Luciana; Calegário,Gabriela; Appolinario,Luciana; Vidal,Lívia; Silva,Bruno S.; Tonon,Luciane A. Chimetto; Tschoeke,Diogo A.; Garcia,Gizele D.; Thompson,Fabiano L.; Thompson,Cristiane C..
Abstract We present here the genome sequence of Shewanella corallii strain A687 isolated from pufferfish Sphoeroides spengleri (Family Tetraodontidae). The assembly consists of 5,215,037 bp and contains 284 contigs, with a G+C content of 50.3%.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Shewanella; Gammaproteobacteria; Genome; Gene prediction; Secondary metabolism.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100401
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Genetic variants in the fat mass and obesity-associated (FTO) gene confer risk for extreme obesity and modulate adiposity in a Brazilian population Genet. Mol. Biol.
Fonseca,Ana Carolina Proença da; Marchesini,Bruna; Zembrzuski,Verônica Marques; Voigt,Danielle Dutra; Ramos,Vivianne Galante; Carneiro,João Regis Ivar; Nogueira Neto,José Firmino; Cabello,Giselda Maria Kalil de; Cabello,Pedro Hernán.
Abstract Obesity is a major public health problem worldwide. It has a complex etiology, influenced by environmental and genetic factors. FTO has been recognized as an important genetic factor for obesity development. This study evaluated the contribution of FTO polymorphisms (rs9939609 and rs17817449) for extreme obesity in terms of the period of obesity onset, anthropometric, and biochemical parameters. The haplotype and the combined effects of FTO risk alleles on obesity susceptibility were evaluated. We investigated 169 normal-weight subjects (body mass index, BMI: 22.8 [21.0; 24.0] kg/m2) and 123 extremely obese individuals (BMI: 47.6 [44.1; 53.1] kg/m2). Genotyping was performed by real time PCR. Our results showed a strong association between FTO...
Tipo: Info:eu-repo/semantics/article Palavras-chave: BMI; Extreme obesity; FTO; Haplotype; Polymorphisms.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100111
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Roles of the mitochondrial replisome in mitochondrial DNA deletion formation Genet. Mol. Biol.
Oliveira,Marcos T.; Pontes,Carolina de Bovi; Ciesielski,Grzegorz L..
Abstract Mitochondrial DNA (mtDNA) deletions are a common cause of human mitochondrial diseases. Mutations in the genes encoding components of the mitochondrial replisome, such as DNA polymerase gamma (Pol γ) and the mtDNA helicase Twinkle, have been associated with the accumulation of such deletions and the development of pathological conditions in humans. Recently, we demonstrated that changes in the level of wild-type Twinkle promote mtDNA deletions, which implies that not only mutations in, but also dysregulation of the stoichiometry between the replisome components is potentially pathogenic. The mechanism(s) by which alterations to the replisome function generate mtDNA deletions is(are) currently under debate. It is commonly accepted that stalling of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mitochondria; DNA replication; Human diseases; Pol γ; Twinkle.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200307
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Potassium chloride fertilization of potatoes: nutrient uptake rate and tuber yield of cultivars Ágata and Atlantic Horticultura Brasileira
Luz,José Magno Q; Oliveira,Roberta C; Lana,Regina Maria Q; Silva,João Ricardo R; Rebouças,Tiyoko Nair H.
ABSTRACT Potassium (K) is the nutrient taken up in the greatest quantity by the potato plant. Obtaining information about the relationship between tuber yield and K application rate allows improvements in fertilizer use efficiency. We aimed to evaluate the variation in potassium fertilizer doses in uptake rate of other nutrients and in potato tuber yield. The experiments were carried out in Unaí-MG testing cultivars Ágata and Atlantic and in Mucugê-BA evaluating cultivar Ágata. The experimental design used was randomized blocks. We studied the rates of 0; 70; 110; 220, and 450 kg ha-1 K2O. The increase in K rate reduced the levels of S, Ca, Mg and B in Atlantic-Unaí, Ca, Mg, Zn and B in Ágata-Unaí and S, Fe and B in Ágata-Mucugê. The cultivar Atlantic-Unaí...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum; Mineral fertilization; Plant nutrition.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000400400
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Evaluation of onion genotypes to slippery skin caused by Burkholderia gladioli pv. Alliicola Horticultura Brasileira
Velez,Leandro S; Silva,Adriano Márcio F; Santos,Carlos AF; Assunção,Emanuel F; Silva,Matheus S; Souza,Elineide B; Gama,Marco Aurélio S.
ABSTRACT Onion’s (Allium cepa) slippery skin caused by Burkholderia gladioli pv. alliicola is one of the bacterial diseases that stand out in producing regions of the Brazilian Northeast. Given the importance and potential threat of this disease, and the absence of resistant varieties, the aim of this study was to evaluate the reaction of onion genotypes to slippery skin and to analyze the tolerance stability of the most promising genotypes to different strains of the bacteria. Nine strains of B. gladioli pv. alliicola were artificially inoculated in onion bulbs cv. Baia Periforme and the three most aggressive strains (CCRMBG39, CCRMBG172 and CCRMBG212) were selected to evaluate the tolerance to the disease. Fifty-eight onion genotypes were challenged with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa; Genetic resistance; Onion bacterial rot.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000400350
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Efficiency of the gravity table in the processing of coriander seeds Horticultura Brasileira
Gadotti,Gizele Ingrid; Hornke,Nander F; Cavalcante,Jerffeson A; Silva,Joseano G da; Gonçalves,Vanessa P; Capilheira,André F.
ABSTRACT This study aimed at assessing the quality of the physiology and health of the coriander seeds processed in a gravity table. This was investigated at two settings namely, I with angle X 3.5° and angle Y 3.1° and II with 3.9° and 2.7° angles, with five gravity table discharges (high, high intermediate, intermediate, low intermediate and low) besides feeding. The experiment adopted the 2x6 factorial scheme, in a completely randomized design, with four replications. Two coriander seed batches of cultivar Verdão were used. The gravity table was adjusted to have 2.9 m s-1 air speed on the platform. The seed fractions discharged in the lower portion of the gravity table revealed poorer physiological performance than that of the others. Regulation I...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coriandrum sativum; Separation; Specific mass; Physiological quality.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000200211
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In vitro culture of jambu with different growth regulators Horticultura Brasileira
Almeida,Susan P; Souza,Joanne MM; Amorim,Andredy MT; Gusmão,Sérgio AL de; Souza,Rodrigo ORM; Santos,Alberdan S.
ABSTRACT The aim of this study was to establish the best concentrations of growth regulators for in vitro cultivation of jambu for a subsequent elaboration of an efficient micropropagation protocol. After sterilized, the seeds were inoculated on different media (MS, ½MS and water-agar) for in vitro germination. Nodal segments of in vitro germinated jambu seedlings were used as explants in the micropropagation with different concentrations of 6-benzylaminopurine (BAP) (0.0; 0.125; 0.25; 0.50 and 0.75 mg L-1) and callus induction with 2.4- dichlorophenoxyacetic acid (2.4-D) (0.0; 0.25; 0.50; 0.75 and 1.0 mg L-1) on Murashige & Skoog’s (MS) medium. The highest germination rates were obtained on MS medium with better seedling development and greater height...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Acmella oleracea; Micropropagation; Callus induction; BAP; 2.4-D; Nodal segments.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000200134
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Tomato production in hydroponic system using different agrofilms as greenhouse cover Horticultura Brasileira
Marques,Douglas José; Matheus Filho,Edmur; Bianchini,Hudson C; Veroneze Junior,Valdir; Santos,Breno Régis; Carlos,Lanamar de A; Silva,Ernani Clarete da.
ABSTRACT Protected cultivation has increased over the years. More studies on the benefits of using photoselective agrofilms are necessary. The choice of material to cover the greenhouse is a decisive factor for crop development and production. The aim of this study was to evaluate the effect of diffuser and blue-colored agrofilms on the production of different tomato cultivars of indeterminate growth habit under NFT hydroponic system. A completely randomized design in a 2x3 factorial scheme (two environments and three cultivars) was used: two agrofilm types (blue and diffuser) and 3 cultivars (Monterrey, Arendell and Totalle). Each plot consisted of 30 plants per treatment, with four replicates. Commercial tomato seedlings of indeterminate growth habit...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lycopersicon esculentum; Lycopene; Gas exchange; Radiation.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000100058
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Physiological potential of onion seeds stored in different packings and environments Horticultura Brasileira
Hornke,Nander F; Gadotti,Gizele I; Capilheira,André F; Cavalcante,Jerffeson A; Nadal,Ariele P; Silva,Joseano G.
ABSTRACT Storage is a fundamental step for maintaining seed quality after processing. Thus, in this study, we evaluated the physiological potential of onion seeds stored in different packings and environments. Impermeable, semi-permeable and permeable packings were tested under natural environment conditions (+19ºC), cold and dry chamber (10±2ºC and 33% RH) and cold chamber (16±2ºC and 60% RH), at 0, 90, 180, 270 and 360 days. The design was completely randomized in a 3x3x5 factorial scheme, with three replicates. In order to evaluate emergency in field, a 3x3 factorial arrangement (packing x environment) was adopted. Onion seeds maintained their physiological quality when stored in impermeable packing for 360 days, regardless of the environment. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa; Hermetic packages; Gas exchange.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300312
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Agronomic performance of arugula/nira intercropping in different cultivation arrangements Horticultura Brasileira
Guimarães,Marcelo de Almeida; Lima Neto,Benedito Pereira; Lemos Neto,Hozano de Souza; Hendges,Ana Régia Alves de Araújo; Viana,Caris dos Santos; Rabelo,Janiquelle da Silva.
ABSTRACT Spatial planning of cultivation arrangements is essential to ensure the superiority of intercropping when compared with monocrop. Thus, the aim of this study was to evaluate the agronomic performance of arugula/nira intercropping in different cultivation arrangements. The experiment was carried out in a randomized block design, in a split plot scheme, the plots represented the cultivation arrangements and the subplots the production cycles (winter and spring). The arrangements were T1= arugula monoculture; T2= two rows of arugula alternating with three rows of nira (2R:3N); T3= two rows of arugula alternating with two rows of nira (2R:2N); T4= two rows of arugula alternating with one row of nira (2R:1N); T5= nira monoculture. Productive traits and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eruca sativa; Allium tuberosum; Efficient use of land; Productivity.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300329
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Effect of humus and soil substrates on production parameters and quality of organic strawberries Horticultura Brasileira
Hoehne,Lucélia; Altmayer,Taciélen; Martini,Maira C; Finatto,Jordana; Brietzke,Débora T; Kuhn,Daniel; Schweizer,Ytan A; Vettorello,Gabriela; Cordeiro,Sabrina G; Ethur,Eduardo M; Freitas,Elisete M de; Severo Filho,Wolmar A.
ABSTRACT This study aims to verify the most suitable substrate formulation based on proportions of earthworm humus and soil to improve the quality of organic strawberries. Vermicomposting generated humus and strawberry seedlings were planted in different humus:soil ratios: (0:100; 20:80; 40:60; 60:40; 80:20 and 100:0, respectively). The proportional conditions were characterized after planting and after 60-day cultivation, plants were harvested, and chemical characterizations of the soil and plants were made. We verified that with an increase of humus content in the substrate, an increase in values of N, P, K, pH and organic matter in soil and dry and fresh mass in shoot area of the plants were noticed. In relation to fruits, pH, humidity and sodium...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fragaria x ananassa; Húmus; Fertilizer; Nutrients.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000100101
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Inoculation of diazotrophic bacteria in chicory Horticultura Brasileira
Braulio,Caliane da S; Nóbrega,Rafaela SA; Silva,Luís Cláudio V; Correia,Andreza de J; Sousa,Cheila BC de; Pereira,Elisângela G.
ABSTRACT Inoculating crops with diazotrophic bacteria constitutes a feasible biotechnological tool to reduce or even avoid the use of nitrogen fertilizers and to improve the nutritional status of crops. However, the potential use of such technology is limited due to the lack of studies in diverse vegetable crops. We evaluated the production of chicory (Cichorium intybus) in response to inoculation with diazotrophic bacteria in two soil classes. The experiments were performed under greenhouse conditions and under a completely randomized design. The treatments were constituted by strains UFRB FA34C2-2, UFLA 03-84 and INPA 0311B, and two control treatments (devoid of bacterial inoculation and with and without nitrogen fertilization). Two experiments were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cichorium intybus; Growth promoting rhizobacteria; Organic production; Sustainability.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000200185
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Evaluation of purple-fleshed sweetpotato genotypes for root yield, quality and pest resistance Horticultura Brasileira
Melo,Raphael Augusto de C; Silva,Giovani Olegario da; Vendrame,Larissa PC; Pilon,Lucimeire; Guimarães,Jorge Anderson; Amaro,Geovani Bernardo.
ABSTRACT Production of purple-fleshed sweetpotatoes, source of anthocyanins, pigments with high antioxidant activities, is expected to be extended during the upcoming years, as there is a strong trend to market products promising improved health. This study aimed to evaluate yield, postharvest quality and pest resistance of roots of purple-fleshed sweetpotatoes. Six genotypes (BGBD 0005, BGBD 0080, BGBD 1261, BGBD 1399, BGBD 1402 and BGBD 1405) and the cultivars Beauregard and Brazlândia Roxa as controls were evaluated during two seasons 2018 (Experiment 1) and 2019 (Experiment 2), in Brasília-DF, Brazil. The experiments were conducted in a complete randomized blocks design with four replications, with experimental plots consisting of two rows of six...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ipomea batatas; Marketable roots; Appearance; Insect damage; Soluble solids; Dry matter content.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000400439
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Production efficiency in cultivar combinations of beet and cowpea Horticultura Brasileira
Ribeiro,Jeisy RS; Bezerra Neto,Francisco; Lima,Jailma SS; Chaves,Aridênia P; Silva,Josimar N; Santos,Elizangela C; Nunes,Renato LC.
ABSTRACT For the simultaneous achievement of productivity in beet and cowpea associations, it is necessary to take into consideration the choice of cultivar combinations that offer a response in terms of production, product quality and efficiency of the intercropping. Thus, the objective of this work was to evaluate the viability of production of beet cultivars combinations with cowpea cultivars in two cropping years (2016 and 2017) in a semi-arid environment. The treatments arranged in a 2 x 4 factorial scheme, with four replications, consisted of the combination of two beet cultivars (Early Wonder and Fortuna) with four cowpea cultivars (BRS Tumucumaque, BRS Cauamé, BRS Guariba and BRS Itaim), in addition to monocultures of these cultivars, as an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Beta vulgaris; Vigna unguiculata; Calotropis procera; Crop association; Semi-arid environment.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000400445
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Ammonium/nitrate ratio affects the growth and glucosinolates content of pakchoi Horticultura Brasileira
Yang,Jing; Zhu,Biao; Ni,Xiaolei; He,Yong.
ABSTRACT Nitrogen (N) strongly affects plant growth. However, little is known about the effects of the ammonium/nitrate ratio on pakchoi (Brassica rapa), especially its glucosinolates (GSs) contents which are involved in plant defense and many of them benefit to human health. The aim of this study was to evaluate the effects of a constant N supply (8 mM) but with five ammonium/nitrate ratios (namely 0/8 mM, 2/6 mM, 4/4 mM, 6/2 mM and 8/0 mM) on the growth of pakchoi in a hydroponic system in 2 years. In both years, a higher biomass (dry weight) was in the 4/4 and 2/6 ammonium/nitrate treatments (2.3 and 2.2-fold compared to 8/0, respectively), with no significant difference in biomass between these two treatments. The biomass then decreased with increasing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brassica rapa; Biomass; Nitrogen form; Secondary metabolites.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300246
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Mixtures between Beauveria bassiana and potassium silicate to manage thrips in tomato plants for industrial processing Horticultura Brasileira
Pereira,Alexandre Igor A; Silva,Cide M da; Curvêlo,Carmen RS; Pontes,Nadson de C; Pereira,Jardel L; Tavares,Wagner de S; Zanuncio,José C; Luz,José Magno Q.
ABSTRACT Thysanoptera species can transmit virus to Solanaceae family vegetables, including tomato plants for industrial processing, causing the disease known as Tomato Spotted Wilt Virus (TSWV). Thrips resistance to insecticides indicate the urgent need of techniques adequate for Integrate Pest Management practices. The objective was to evaluate the control efficiency (CE%) against Frankliniella schultzei (Thysanoptera: Thripidae) of the fungus Beauveria bassiana (Bb), the resistance inducer potassium silicate (KSil) and the chemical insecticide profenophos + cypermethrin (PC) isolated, or in binary mixtures. The treatments were foliar spraying on tomato plants with Bb (T1), KSil (T2) and PC (T3) isolated or in mixtures of Bb+KSil (T4), PC+KSil (T5) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Frankliniella schultzei; Solanum lycopersicum; Entomopathogenic fungi; Silicon; Synergism; Tank mixture.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000400415
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Resistance sources to root-knot nematodes Meloidogyne javanica, M. incognita and M. enterolobii in sweet potato Horticultura Brasileira
Carmona,Paula Andrea O; Pinheiro,Jadir B; Amaro,Geovani Bernardo; Silva,Giovani Olegario da; Peixoto,José Ricardo; Cares,Juvenil Enrique.
ABSTRACT One of the main obstacles for food production in many developing countries, as in Brazil, is the damage caused by root-knot nematodes, mainly those belonging to the genus Meloidogyne. This study aimed to assess the resistance levels of 44 sweet potato genotypes to M. javanica, M. incognita race 1 and M. enterolobii. These researches were carried out in 2014, under greenhouse conditions in Brasília-DF, Brazil. A completely randomized design with six replicates of one plant/plot/treatment was used. We determined the gall index (GI) and egg mass index (EMI) in the root system of each plant, the number of eggs and juveniles per gram of root with galls and the nematode reproduction factor. M. javanica was less aggressive and reproduced in only 9.09% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ipomoea batatas; Plant breeding; Reproduction factor; Genetic resistance.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000200126
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Chemical stress reduces the lateral shoot growth in vernalized garlic Horticultura Brasileira
Gabriel,André; Resende,Juliano TV; Marodin,Josué C; Matos,Rafael de; Zeist,André R; Maciel,Cleber Daniel G.
ABSTRACT Brazil’s low garlic productivity is attributed, mainly to the lateral shoot growth, a physiological anomaly characterized by early lateral shoot growth of cloves in noble group garlic cultivars. Considering the aforementioned information, the aim was to evaluate the occurrence of lateral shoot growth and productivity characteristics in garlic plants, cv. Chonan, subject to herbicide chemical stress. In order to do so, an experiment was conducted in outlining randomized blocks in a 3x5 factorial scheme, constituted by three herbicides (paraquat 500 g ha-1; ammonium gluphosinate 400 g ha-1 and glyphosate 720 g ha-1) and five subdoses (0, 5, 10, 15 and 20% of the minimum recommended dose of each herbicide). The electron transport rates (ETR) were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium sativum; Herbicide; Electron transport rate.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000100041
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