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| Registros recuperados: 57 | |
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| TAGAWA, Michihito; YBANEZ, Adrian P.; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions... |
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Palavras-chave: Bovine hemoplasma; Direct PCR; Japan; Risk factor. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3838 |
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| 松本, 高太郎; 村上, 智亮; 菅生, 樹春; 山田, 一孝; 古林, 与志安; 松井, 高峯; 猪熊, 壽; MATSUMOTO, Kotaro; MURAKAMI, Tomotaka; SUGO, Kiharu; YAMADA, Kazutaka; KOBAYASHI, Yoshiyasu; MATSUI, Takane; INOKUMA, Hisashi. |
| 17日齢のホルスタイン種雌子牛が，神経症状を示した. 病畜は頭部を左後方に折り曲げて伏臥姿勢を示し，時折自力で起立したがすぐに倒れ歩行不能であった. 神経学的検査では右の対光反射，威嚇瞬き反射，眼瞼反射， および音響耳介反射が消失していた. ビタミンB1，およびデキサメサゾンの投与により第7病日に歩行可能となったものの左への斜頸・旋回が認められ，神経学的検査所見に改善は認められなかった. 第7病日に病理解剖を行い，肉眼的には橋の断面右側に直径1cm大の膿瘍を認め，嫌気培養にてFusobacterium necrophorumが検出された. 左側への斜頸，聴覚の消失等の脳神経疾状は，橋の右側の膿瘍により内耳神経等の脳神経核が圧迫された結果出現した聴覚障害，平衡障害のためと考えられた. A 17-day-0ld female Holstein calf exhibited the following neurological symptoms: the calf bent its neck to the left，turning its face backward and could stand alone，but would fall within a short time. Neurological examination further revealed losses of pupillary light reflex， menace response，palpebral reflex，and hearing on the right side. After administration of dexamethasone and vitamin B1，the calf was able to walk... |
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Palavras-chave: 脳幹部膿瘍; 牛; Fusobacterium necrophorum; Brain stem abscess; Cattle. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2945 |
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| TAGAWA, Michihito; YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi; 滄木, 孝弘; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽. |
| The present study evaluated the effect of hemoplasmosis on cattle productivity. Prevalence of bovine hemoplasma was examined by polymerase chain reaction (PCR) using whole blood samples collected from 93 breeding cows and their 71 calves in Hokkaido, Japan. Monthly milk production records and other clinical data were compared between Mycoplasma wenyonii (Mw)-infected, “Candidatus Mycoplasma haemobos” (CMh)-infected, co-infected and PCR-negative groups. Blood chemical parameters were obtained from the 93 cows and 64 calves. PCR results showed that 89.2% (83/93) of cows and 14.1% (10/71) of calves were positive for bovine hemoplasma. Based on productivity data obtained from the 93 cows, Mw-infected, CMh-infected and co-infected cows had significantly lower... |
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Palavras-chave: Cattle; Direct PCR; Hemoplasma; Productivity. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3870 |
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| KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明. |
| Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3). |
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Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585 |
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| Sakamoto, Leo; Ichikawa, Yasuaki; Sakata, Yoshimi; Matsumoto, Kotaro; Inokuma, Hisashi; 松本, 高太郎; 猪熊, 壽. |
| The prevalence of Ehrlichia and Anaplasma in 1,427 dogs from 32 Japanese prefectures was evaluated by PCR and DNA nucleotide sequencing. PCR screening demonstrated that 18 dogs (1.3%) were positive for Anaplasmataceae. Sequence analysis revealed that 14 of the amplicons were most closely related to Wolbachia spp., symbionts of Dirofilaria immitis, whereas three were identified as Anaplasma bovis. The remaining amplicon could not be sequenced. Almost the entire sequence of 16S rRNA (1,452 bp) from one of the positive specimens was determined, and subsequent phylogenetic analysis confirmed that the detected sequence was that of A. bovis. This is the first detection of A. bovis DNA fragments in dogs. Species-specific nested PCR showed that 15 (1.1%) of the... |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3083 |
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| 猪熊, 壽; 滄木, 孝弘; 山川, 和宏; 竹内, 俊彦; 松本, 高太郎; 石井, 三都夫; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; YAMAKAWA, Kazuhiro; TAKEUCHI, Toshihiko; MATSUMOTO, Kotaro; ISHII, Mitsuo; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi. |
| 病理学的に血様液貯留を特徴とする特発性心嚢水腫と診断された乳牛8症例（血様心嚢水貯留群）の臨床病理学的検査所見を回顧的に取りまとめ，心膜炎7症例（心膜炎群）の所見と比較した．血様心嚢水貯留群では冷性浮腫を呈する症例が心膜炎群に比べて多かったが，その他の臨床症状には両群で差がなかった．心エコー検査では血様心嚢水貯留群では，心膜炎でみられる心嚢内の顕著なフィブリン析出はなかった．血液及び生化学検査では，血様心嚢水貯留群では炎症像が弱く，心膜炎群と比較して白血球数，分葉核好中球割合，A/G比，アルブミン濃度，γグロブリン割合に有意差がみられた．両群でALP及びGGTの高値がみられたが，特に血様心嚢水貯留群のGGT増加は顕著であった．血様心嚢水貯留症例の診断は臨床症状だけでは困難であるが，心エコー検査所見，弱い炎症像，ALP及びGGTの高値などが心膜炎との鑑別診断に有用と考えられた． Clinicopathological findings of eight cases of idiopathic hydropericardium characterized by bloodhlike fluid (HBFhgroup) that were confirmed by necropsy were retrospectively summarized and compared with those of seven cows with pericarditis. Although the numbers of cows that showed edema in the HBFhgroup were larger than those in the pericarditishgroup, the frequency of... |
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Palavras-chave: 特発性心餒水腫; 心膜炎; 血様心嚢水; 牛; Idiopathic hydropericardium; Pericarditis; Blood-like pericardium; Cow. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3557 |
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| 佐々木, 直樹; 山田, 一考; 伊藤, めぐみ; 柳川, 将志; 松井, 基純; 羽田, 真悟; 松本, 高太郎; 堀内, 雅之; 南保, 泰雄; 猪熊, 壽. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4506 |
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| 田川, 道人; 大橋, 英二; 本村, 絹代; 千葉, 史織; 堀内, 雅之; 三好, 雅史; 猪熊, 壽; 松本, 高太郎; TAGAWA, Michihito; OHASHI, Eiji; MOTOMURA, Kinuyo; CHIBA, Shiori; HORIUCHI, Noriyuki; MIYOSHI, Masahumi; INOKUMA, Hisashi; MATSUMOTO, Kotaro. |
| 10歳の雄の雑種犬が，3週間前から持続する発熱及び元気消失を主訴に帯広畜産大学動物医療センターを受診した．心雑音及び心エコー検査において僧帽弁の疣贅物が確認され，抗生物質等により加療したが，第7病日より心不全の悪化を認め，第10病日に肺水腫により斃死した．病理解剖では，軽度の心拡大及び僧帽弁における多中心性疣贅物が認められた．血液培養検査では，Staphylococcus haemolyticusが検出されたが，疣贅物を用いたPCR検査ではStreptococcus canisと100％一致する塩基配列が得られた．血液培養分離菌はコンタミネーションであった可能性が高く，本症例の心内膜炎はStreptococcus canisによるものと考えられた．血液培養結果と実際の心内膜炎起因菌が異なる場合があることを考慮する必要がある． |
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Palavras-chave: 心内膜炎; PCR; Streptococcus canis. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4232 |
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| TAGAWA, Michihito; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Hemotropic mycoplasmas (hemoplasmas) are cell-wall deficient, epierythrocytic bacteria that cause infectious anemia in several mammalian species. The prevalence of hemoplasma species was examined by screening and species-specific PCR using blood samples collected from 51 sika deer in Hokkaido, Japan. Molecular analyses were performed for the 16S rRNA, 23S rRNA and RNase P RNA (rnpB) gene sequences. A total of 23/51 (45%) deer DNA samples were positive for hemoplasmas in the screening PCR. Using species-specific PCR, 12 and 17 samples were positive for ‘Candidatus Mycoplasma haemocervae’ and ‘Candidatus M. erythrocervae’, respectively. Sequencing and phylogenetic trees of those three genes indicate that the ‘Candidatus M. haemocervae’ and ‘Candidatus M.... |
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Palavras-chave: Cervus nippon yesoensis; Hemoplasma; Japan; Phylogeny. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4012 |
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| YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; BATTSETSEG, Badgar; BATTUR, Banzragch; ALTANGEREL, Khukhuu; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA... |
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Palavras-chave: Anaplasma marginale; Cattle; Mongolia; Msp5; PCR. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3867 |
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| Sasaki, Hiromi; Goyama, Takashi; Noda, Yoichiro; Matsumoto, Kotaro; Kobayashi, Yoshiyasu; Inokuma, Hisashi; 松本, 高太郎; 古林, 与志安; 猪熊, 壽. |
| A 2-month-old male Holstein calf showed clinical signs of abdominal bloating, melena, and pain and was suspected of having a perforating abomasal ulcer. Necropsy revealed a large mass located preferentially around the abomasum and a large perforating abomasal ulcer on the pyloric antrum. Milky white masses of various sizes were also found in the abdominal cavity that consisted of agglutinated nodules ranging in size from a pinhead to a golf ball and were distributed on the surfaces of the liver, reticulum, omasum, abomasum, and diaphragm. Microscopic examination revealed that the masses were composed primarily of hyaline matrices, epithelioid tumor cells, and large atypical cells with hyaline droplets and/or vacuoles. Stromal hyaline matrices and hyaline... |
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Palavras-chave: Holstein calf; Perforating abomasal ulcer; Yolk sac tumor. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3824 |
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| HORIUCHI, Noriyuki; KOMAGATA, Makoto; SHITAMURA, Keiichi; CHIBA, Shiori; MATSUMOTO, Kotaro; INOKUMA, Hisashi; MATSUI, Takane; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古林, 与志安. |
| An 11-year-old Holstein-Friesian cow exhibited anorexia and jaundice. A large mass was found in the liver during necropsy. Macroscopically, the mass was composed of dark red multilobular tissue and a centrally located abscess, which was connected to the hepatic duct. Histologically, the mass consisted of proliferation of small neoplastic cells and was demarcated from the hepatic parenchyma by a thick region of granulation tissue. The neoplastic cells were predominantly arranged in solid sheets, but they also formed blood-filled cancellous structures, and proliferating foci were seen around blood vessels. Periodic acid-Schiff reaction demonstrated that a fine basement membrane-like structure surrounded the neoplastic cells. Immunohistochemically, the... |
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Palavras-chave: Cholangitic abscess; Cow; Glomus tumor. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4172 |
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| Registros recuperados: 57 | |
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