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| Registros recuperados: 43 | |
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| Huvet, Arnaud; Beguel, Jean-philippe; Pereira Cavaleiro, Nathalia; Thomas, Yoann; Quillien, Virgile; Boudry, Pierre; Alunno-bruscia, Marianne; Fabioux, Caroline. |
| Feeding strategies and digestive capacities can have important implications for variation in energetic pathways associated with ecological and economically important traits, such as growth or reproduction in bivalve species. Here, we investigated the role of amylase in the digestive processes of Crassostrea gigas, using in vivo RNA interference. This approach also allowed us to investigate the relationship between energy intake by feeding and gametogenesis in oysters. Double-stranded (ds)RNA designed to target the two α-amylase genes A and B was injected in vivo into the visceral mass of oysters at two doses. These treatments caused significant reductions in mean mRNA levels of the amylase genes: −50.7% and −59% mRNA A, and −71.9% and −70.6% mRNA B in 15... |
| Tipo: Text |
Palavras-chave: Alpha-Amylase; DsRNA; Gametogenesis; Marine bivalve; Dynamic energy budget. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00271/38239/36422.pdf |
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| Strohmeier, Tore; Strand, Øivind; Aure, Jan; Duinker, Arne; Alunno-bruscia, Marianne; Erga, Svein Rune; Rosland, R.; Naustvoll, Lars. |
| Objectives The objective of the study is to assess how phytoplankton composition and quantity enhanced by forced upwelling of nutrient rich deep water in a fjord affects growth performance and detoxification in cultured mussels. Sub goals are: 1. monitor the fjord environment and succession of phytoplankton including toxic algae, 2. contrast feeding and growth performance in mussels affected and unaffected by the enhanced phytoplankton assemblage forced by upwelling of nutrient rich deep water, 3. estimate elimination rate of algae toxins in mussels introduced to enhanced phytoplankton concentration forced by upwelling of nutrient rich deep water. |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00033/14376/11666.pdf |
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| Thomas, Yoann; Mazurie, Joseph; Alunno-bruscia, Marianne; Bacher, Cedric; Bouget, Jean-francois; Gohin, Francis; Pouvreau, Stephane; Struski, Caroline. |
| In order to assess the potential of various marine ecosystems for shellfish aquaculture and to evaluate their carrying capacities, there is a need to clarify the response of exploited species to environmental variations using robust ecophysiological models and available environmental data. For a large range of applications and comparison purposes, a non-specific approach based on ‘generic’ individual growth models offers many advantages. In this context, we simulated the response of blue mussel (Mytilus edulis L.) to the spatio-temporal fluctuations of the environment in Mont Saint-Michel Bay (North Brittany) by forcing a generic growth model based on Dynamic Energy Budgets with satellite-derived environmental data (i.e. temperature and food). After a... |
| Tipo: Text |
Palavras-chave: Mussel; DEB; SeaWifs; Aquaculture; Site Selection; Resource Management. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00037/14836/14537.pdf |
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| Alunno-bruscia, Marianne; Van Der Vee, Henk W.; Kooijman, S. A. L. M.. |
| This second special issue of the Journal of Sea Research on development and applications of Dynamic Energy Budget (DEB) theory concludes the European Research Project AquaDEB (2007–2011). In this introductory paper we summarise the progress made during the running time of this 5 years’ project, present context for the papers in this volume and discuss future directions. The main scientific objectives in AquaDEB were (i) to study and compare the sensitivity of aquatic species (mainly molluscs and fish) to environmental variability within the context of DEB theory for metabolic organisation, and (ii) to evaluate the inter-relationships between different biological levels (individual, population, ecosystem) and temporal scales (life cycle, population... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00044/15497/14508.pdf |
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| Pouvreau, Stephane; Petton, Sebastien; Queau, Isabelle; Haurie, Axel; Le Souchu, Pierrick; Alunno-bruscia, Marianne; Palvadeau, Hubert; Auby, Isabelle; Maurer, Daniele; D'Amico, Florence; Passoni, Sarah; Barbier, Claire; Tournaire, Marie-pierre; Rigouin, Loic; Rumebe, Myriam; Fleury, Elodie; Fouillaron, Pierre; Bouget, Jean-francois; Pepin, Jean-francois; Robert, Stephane; Grizon, James; Seugnet, Jean-luc; Chabirand, Jean-michel; Le Moine, Olivier; Guesdon, Stephane; Lagarde, Franck; Mortreux, Serge; Le Gall, Patrik; Messiaen, Gregory; Roque D'Orbcastel, Emmanuelle; Quemener, Loic; Repecaud, Michel; Mille, Dominique; Geay, Amelie; Bouquet, Anne-lise. |
| La conchyliculture, et principalement l’élevage de l’huître creuse, Crassostrea gigas, constitue la principale activité aquacole française. Cette activité repose, en grande partie, sur le recrutement naturel de l’espèce qui assure 70% des besoins en jeunes huîtres (naissain) : cette activité de collecte s’appelle le captage. Les deux principaux centres de captage en France sont les bassins d’Arcachon et de Marennes-Oléron. Or, depuis une dizaine d'années, sur le bassin d'Arcachon, le captage devient très variable: à des années de captage nul (par exemple les années 2002, 2005, 2007) ou faible (2009, 2010, 2011) succèdent des années excellentes voire pléthoriques (les années 2003, 2006, 2008, 2012, 2013). A Marennes-Oléron, cette variabilité existe, mais... |
| Tipo: Text |
Palavras-chave: Huître creuse; Naissain; Recrutement; Captage; Larves; Cycle de reproduction; Changement climatique; Pacific oyster; Spat; Recruitment; Spat collecting; Larvae; Reproductive cycle; Climate change. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00279/38990/37561.pdf |
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| Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre; Alunno-bruscia, Marianne. |
| Since 2008, mass mortalities of 1-yr-old oysters Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have occurred along all coasts of France. These mortality events represent the most serious crisis for the French oyster industry since the introduction of C. gigas in the early 1970s. Temperature is commonly one of the major triggering factors of disease epizootics, notably for aquatic species. In France, OsHV-1 is generally detected in dying oysters when seawater temperature is higher than 16° C. The influence of temperature on OsHV-1 detection and expression was also demonstrated for C. gigas larvae and suspected for juveniles. The present study aims to characterize the effect of temperature on disease transmission and survival related... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00151/26259/24343.pdf |
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| Castrec, Justine; Hegaret, Helene; Alunno-bruscia, Marianne; Picard, Mailys; Soudant, Philippe; Petton, Bruno; Boulais, Myrina; Suquet, Marc; Queau, Isabelle; Ratiskol, Dominique; Foulon, Valentin; Le Goic, Nelly; Fabioux, Caroline. |
| Harmful algal blooms are a threat to aquatic organisms and coastal ecosystems. Among harmful species, the widespread distributed genus Alexandrium is of global importance. This genus is well-known for the synthesis of paralytic shellfish toxins which are toxic for humans through the consumption of contaminated shellfish. While the effects of Alexandrium species upon the physiology of bivalves are now well documented, consequences on reproduction remain poorly studied. In France, Alexandrium minutum blooms have been recurrent for the last decades, generally appearing during the reproduction season of most bivalves including the oyster Crassostrea gigas. These blooms could not only affect gametogenesis but also spawning, larval development or juvenile... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom (HAB); Paralytic shellfish toxin (PST); Crassostrea gigas; Gametes; Larvae. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00469/58099/60512.pdf |
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| Wegner, K. Mathias; Piel, Damien; Bruto, Maxime; John, Uwe; Mao, Zhijuan; Alunno-bruscia, Marianne; Petton, Bruno; Le Roux, Frederique. |
| Bacteria of the Vibrio genus are the most predominant infectious agents threatening marine wildlife and aquaculture. Due to the large genetic diversity of these pathogens, the molecular determinants of Vibrio virulence are only poorly understood. Furthermore, studies tend to ignore co-evolutionary interactions between different host populations and their locally encountered Vibrio communities. Here, we explore the molecular targets of such co-evolutionary interactions by analyzing the genomes of nine Vibrio strains from the Splendidus-clade showing opposite virulence patterns towards two populations of Pacific oysters introduced into European Wadden Sea. By contrasting Vibrio phylogeny to their host specific virulence patterns, we could identify two core... |
| Tipo: Text |
Palavras-chave: Virulence; Host-pathogen interaction; Local adaptation; Biological invasion; Emerging disease; Wadden Sea. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00512/62409/66669.pdf |
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| Petton, Bruno; Riobe, Marion; Koechlin, Hugo; Queau, Isabelle; Mingant, Christian; Ratiskol, Dominique; Le Grand, Jacqueline; Petton, Sebastien; Thomas, Yoann; Alunno-bruscia, Marianne; Pouvreau, Stephane; Pernet, Fabrice; Maurer, Daniele; D'Amico, Florence; Lagarde, Franck; Mortreux, Serge; Roque D'Orbcastel, Emmanuelle; Mille, Dominique; Geay, A.; Bouquet, A.-l.; Cochet, Helene. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Des travaux de qualification zoo-sanitaire menés depuis 2010 ont montré qu’un lot de naissains issus du captage naturel sur deux était infecté par le virus bien avant l’apparition des mortalités dans le milieu naturel, mais qu’a contrario les naissains d’écloserie expertisés présentaient un moindre risque de contamination. Dans le contexte de crise sanitaire chronique, l’objectif de notre étude était d’ obtenir une information qualitative relative au statut zoo-sanitaire OsHV -1 μ V ar des... |
| Tipo: Text |
Palavras-chave: Huitre creuse; OsHV - 1μVar; Epreuve thermique.; Maladie; Naissain; Pacific oyster; Spat; Disease; Thermal challenge; OsHV-1 μVar. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00276/38682/37180.pdf |
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| Strohmeier, Tore; Strand, Oivind; Alunno-bruscia, Marianne; Duinker, Arne; Rosland, Rune; Aure, Jan; Erga, Svein R.; Naustvoll, Lars J.; Jansen, Henrice M.; Cranford, Peter J.. |
| The controlled upwelling of nutrient-rich deep water in oligotrophic coastal regions has been proposed as a means of increasing phytoplankton and, subsequently, bivalve aquaculture production. This was tested as part of a large-scale upwelling experiment in an oligotrophic environment (Lysefjord, Norway). The mean chlorophyll a concentration in the upwelling area was consistently higher than at the control site (mean ± SD: 3.3 ± 1.9 and 1.5 ± 0.6 mg Chl a m-3, respectively) during the 4 mo of controlled upwelling. After 2 mo with upwelling, the dry flesh weight of 1 yr-old and 2 yr-old mussels was 95% and 24% higher, respectively, than that of the mussels at the control site. The 1 yr-old mussels at the upwelling site achieved up to 2.4-fold higher dry... |
| Tipo: Text |
Palavras-chave: Bivalve aquaculture; Production carrying capacity; Physiology; Fjord ecosystems; Shell growth; Tissue growth. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00241/35200/33704.pdf |
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| Alunno-bruscia, Marianne; Rosland, Rune; Strand, Øivind; Naustvoll, Lars; Robert, Stephane; Bacher, Cedric. |
| Introduction The blue mussel (Mytilus edulis) culture occurs in temperate waters around the world under a wide range of environmental conditions, e.g. at phytoplankton concentrations below as 1-2 µg L-1 (in Norwegian fjords) up to 9-10 µg L-1 (in French Atlantic coastal sites). Under such contrasted food resources, the aim of our study is to develop a generic bio-energetic model for M. edulis, i.e. that can be applied in various contrasted environments with a constant set of parameters, to simulate growth and reproduction of the blue mussel. Such a model will allow us to assess directly the links between mussel growth performances and environmental parameters in different culture sites. |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00032/14362/11664.pdf |
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| Petton, Bruno; Boudry, Pierre; Alunno-bruscia, Marianne; Pernet, Fabrice. |
| Mortalities of oyster Crassostrea gigas seed associated with ostreid herpesvirus OsHV-1 μVar have been observed in many oyster-producing countries since 2008. The present study, comprised of 4 complementary experiments, aimed to identify factors associated with disease-induced oyster mortality in order to propose mitigation strategies. Our first experiment compared survival of oysters from natural spatfall with others sampled from nurseries, after thermal elevation in the laboratory from <14 to 21°C. A total of 60% of the tested wild seed batches (n = 51) were infected by OsHV-1, exhibited mortality and were able to transmit the disease to cohabited naïve oysters. Comparatively, only 1 out of the 32 tested batches sampled from nurseries presented... |
| Tipo: Text |
Palavras-chave: Epidemiology; Risk analyses; Shellfish farming; Virus OsHV-1 mu Var. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00257/36777/35390.pdf |
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| Pouvreau, Stephane; Petton, Sebastien; Queau, Isabelle; Bellec, Gwenael; Talarmain, Eric; Le Souchu, Pierrick; Alunno-bruscia, Marianne; Auby, Isabelle; Maurer, Daniele; D'Amico, Florence; Passoni, Sarah; Barbier, Claire; Tournaire, Marie-pierre; Rigouin, Loic; Rumebe, Myriam; Fleury, Elodie; Langlade, Aime; Bouget, Jean-francois; Collin, Karine; Fortune, Mireille; Palvadeau, Hubert; Bedier, Edouard; Robert, Stephane; Pepin, Jean-francois; Grizon, James; Seugnet, Jean-luc; Chabirand, Jean-michel; Le Moine, Olivier; Guesdon, Stephane; Lagarde, Franck; Mortreux, Serge; Le Gall, Patrik; Messiaen, Gregory; Roque D'Orbcastel, Emmanuelle; Quemener, Loic; Repecaud, Michel; Bucas, Karenn; Barbot, Stephane; Guillemot, Anne; Nonnotte, Lidwine; Duros, Wenceslas; Bonnat, Armel; Tarot, Stephane; Mille, Dominique; Geay, Amelie; Bouquet, Anne-lise. |
| La conchyliculture, et principalement l’élevage de l’huître creuse, Crassostrea gigas, constitue la principale activité aquacole française. Cette activité repose, en grande partie, sur le recrutement naturel de l’espèce qui assure 70% des besoins en jeunes huîtres (naissain) : cette activité de collecte s’appelle le captage. Les deux principaux centres de captage en France sont les bassins d’Arcachon et de Marennes-Oléron. Or, depuis une dizaine d'années, sur le bassin d'Arcachon, le captage devient très variable: à des années de captage nul (par exemple les années 2002, 2005, 2007) ou faible (2009, 2010, 2011) succèdent des années excellentes voire pléthoriques (les années 2003, 2006, 2008, 2012, 2013). A Marennes-Oléron, cette variabilité existe, mais... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00200/31091/29499.pdf |
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| Strohmeier, Tore; Strand, Oivind; Alunno-bruscia, Marianne; Duinker, Arne; Cranford, Peter J.. |
| Seasonal variation in blue mussel, Mytilus edulis, retention efficiency (RE) responses to natural seston concentrations were investigated in the Lysefjord, Norway. The smallest particles shown to be effectively retained (100%) were approximately 7 μm and this was observed on only one occasion. The most common response was for RE to progressively increase from small to large particles with maximum RE at 30 to 35 μm (8 of 12 experiments). Temporal changes in the seston size distribution towards a dominance of smaller particles coincided with a decrease in the RE maxima to particles between 7 and 15 μm diameter. The RE of 1 μm and 4 μm diameter particles ranged between 14–64% and 12–86%, respectively, showing that small particles occasionally serve as a... |
| Tipo: Text |
Palavras-chave: Bivalve feeding physiology; Retention efficiency; Clearance rate; Marine ecophysiology; Seston; Suspension feeding; Mytilus edulis. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00054/16509/14024.pdf |
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| Alunno-bruscia, Marianne; Bourget, Edwin; Frechette, Marcel. |
| We examined the influence of food availability and population density on the morphometry and shell length body mass relationship of Mytilus edulis. Mussels were reared in the laboratory for 22 mo at 8 different density levels in 11 chambers supplied with natural seston at 2 different concentrations. This allowed us to assess separately the effects of food availability and mussel density. The shell length/width and shell height/width ratios were affected by food, density and time. Mussels tended to be narrower (flatter) at high density and at low food level. Therefore, narrow shells could result from reduced food concentration in high density situations without implying physical interference. Shell mass was also influenced by both food and density levels,... |
| Tipo: Text |
Palavras-chave: M N curve; Length mass density relationship; Food regulation; Shell allometry; Mussels. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-807.pdf |
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| Pecquerie, Laure; Fablet, Ronan; De Pontual, Helene; Bonhommeau, Sylvain; Alunno-bruscia, Marianne; Petitgas, Pierre; Kooijman, Sebastiaan A. L. M.. |
| Environmental conditions experienced by aquatic organisms are archived in biogenic carbonates such as fish otoliths, bivalve shells and coral skeletons. These calcified structures present an accretionary growth and variations in optical properties - color or opacity - that are used to reconstruct time. Full and reliable exploitation of the information extracted from these structures is, however, often limited as the metabolic processes that control their growth and their optical properties are poorly understood. Here, we propose a new modeling framework that couples both the growth of a biogenic carbonate and its optical properties with the metabolism of the organism. The model relies on well-tested properties of Dynamic Energy Budget (DEB) theory. It is... |
| Tipo: Text |
Palavras-chave: Otolith; Calcification; Metabolism; Bioenergetic model; Food reconstruction; Dynamic Energy Budget theory. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00060/17082/14593.pdf |
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| Emmery, Antoine; Lefebvre, Sebastien; Alunno-bruscia, Marianne; Kooijman, S. A. L. M.. |
| We studied the dynamics of stable isotopes δ13C and δ15N of an opportunistic suspension feeder the Pacific oyster (Crassostrea gigas) to better understand the factors that influence the trophic enrichment (trophic-shift, Δ) between primary producers and consumers. Most of the previous studies on this topic do not quantify mass fluxes or isotopic discrimination phenomena in the organism, which are two pillars in isotope ecology. We used a dynamic energy budget (DEB) approach (Kooijman, 2010) to quantify i) the fluxes of elements and isotopes in C. gigas soft tissues and ii) the impact of the scaled feeding level, the organism mass and the isotopic ratio of food on the "trophic-shift" Δ, and isotope turnover in tissues. Calibration and parametrization... |
| Tipo: Text |
Palavras-chave: Oyster; Isotopic ratio; Discrimination; Trophic-shift; Diet; DEB theory. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15434/12784.pdf |
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| Langlet, D; Alunno-bruscia, Marianne; Rafelis, M; Renard, M; Roux, M; Schein, E; Buestel, Dominique. |
| We present a cathodoluminescence (CL) study of growth layer deposition in the shell of the oyster Crassostrea gigas. CL is based on the physical properties of lattice-bound manganese (Mn2+), which is the main activator in calcium carbonate. Our study involved chemical marking by immersing individuals in seawater to which manganese chloride had been added, and subsequent reading of the shell with CL microscopy coupled with numeric treatment of microphotographs; CL emission was analyzed using a scanning electron microscope coupled to a spectrometer. Since the marking did not harm the oysters, repeated markings were possible, allowing validation of the inferences made from analysis of the shell rings. Oysters reared in a French Mediterranean lagoon (Thau... |
| Tipo: Text |
Palavras-chave: Mediterranean lagoon; Manganese marking; Carbonate biomineraliation; Shell growth; Cathodoluminescence; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1979.pdf |
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| Pousse, Emilien; Flye-sainte-marie, Jonathan; Alunno-bruscia, Marianne; Hegaret, Helene; Rannou, Eric; Pecquerie, Laure; Marques, Goncalo M.; Thomas, Yoann; Castrec, Justine; Fabioux, Caroline; Long, Marc; Lassudrie, Malwenn; Hermabessiere, Ludovic; Amzil, Zouher; Soudant, Philippe; Jean, Fred. |
| As other filter-feeders, Crassostrea gigas can concentrate paralytic shellfish toxins (PST) by consuming dinoflagellate phytoplankton species like Alexandrium minutum. Intake of PST in oyster tissues mainly results from feeding processes, i.e. clearance rate, pre-ingestive sorting and ingestion that are directly influenced by environmental conditions (trophic sources, temperature). This study aimed to develop a mechanistic model coupling the kinetics of PST accumulation and bioenergetics in C. gigas based on Dynamic Energy Budget (DEB) theory. For the first time, the Synthesizing Units (SU) concept was applied to formalize the feeding preference of oysters between non-toxic and toxic microalgae. Toxin intake and accumulation were both dependent on the... |
| Tipo: Text |
Palavras-chave: Alexandrium minutum; Paralytic shellfish toxins (PST); Dynamic Energy Budget (DEB); Modelling; Pacific oyster. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00455/56662/58407.pdf |
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| Registros recuperados: 43 | |
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