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| Registros recuperados: 63 | |
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| Cosson, J; Groison, A; Suquet, Marc; Fauvel, Christian; Dreanno, Catherine; Billard, R. |
| After along period of spermatogenesis (several weeks to months), marine fish spermatozoa are delivered at male spawning in seawater (SW) at the same time as ova. In some fish species, as the ova micropyle closes quickly after release, these minute unicells, the spermatozoa, have to accomplish their task of reaching the micropyle within a very brief period (several seconds to minutes), for delivery of the haploid male genetic information to the ova. To achieve this goal, their high-performance motile equipment, the flagellum, must fully activate immediately on contact with the SW and then propel the sperm cell at an unusually high initial velocity. The cost of such 'hyperactivity' is a very rapid consumption of intracellular ATP that outstrips the supply.... |
| Tipo: Text |
Palavras-chave: Cell activation; Osmolality; Ions; Cell energetics; Motility; Spermatozoon; Marine fish. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6967.pdf |
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| Suquet, Marc; Omnes, Marie-helene; Normant, Yvon; Petton, Bruno; Severe, Armelle; Fauvel, Christian; Barone, Herve; Quemener, Loic; Buchet, Vincent; Pasco, Laurent; Menard, E; Gaignon, Jean-louis. |
| Because of its rapid growth, its good reputation and the promising aquaculture experiences conducted in Norway and Scotland, cod is considered as a good candidate for cold water aquaculture. The aim of this paper is to present the work carried out in France since 1999: the selection of cod as a promising candidate for aquaculture and the assessment of its rearing performances in this environment. |
| Tipo: Text |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00170/28142/26362.pdf |
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| Abascal, F; Cosson, J; Fauvel, Christian. |
| Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (< 50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating... |
| Tipo: Text |
Palavras-chave: Sperm; Heavy metals; Dicentrarchus labrax; CASA. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4765.pdf |
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| Zupa, R.; Fauvel, Christian; Mylonas, C. C.; Pousis, C.; Santamaria, N.; Papadaki, M.; Fakriadis, I.; Cicirelli, V.; Mangano, S.; Passantino, L.; Lacalandra, G. M.; Corriero, Aldo. |
| The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July).... |
| Tipo: Text |
Palavras-chave: Germ cell apoptosis; Germ cell proliferation; Greater amberjack; Rearing in captivity; Seriola dumerili; Sperm quality. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50613/55414.pdf |
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| Fauvel, Christian; Suquet, Marc; Severe, Armelle; Mylonas, Constantinos C.; Papandroulakis, Nikos. |
| The potential of wreckfish (Polyprion americanus) to breed in captivity was examined. Ovarian activity varied seasonally, with vitellogenesis beginning in November and ending in May. Slow-release implant with gonadotropin-releasing hormone agonist (GnRHa) prevented atresia, and both GnRHa injections or implants at the end of vitellogenesis triggered ovulation after 7 days. Spontaneous spawning was obtained only in one female, and the few eggs produced were of poor viability. Manual stripping and artificial fertilization produced viable gametes and embryos. Therefore, present captivity conditions inhibited final oocyte maturation (FOM) and spawning, but not vitellogenesis and spermatogenesis. GnRHa treatments stimulate FOM and ovulation of viable eggs. |
| Tipo: Text |
Palavras-chave: Aquaculture; Polyprion americanus; Wreckfish; GnRHa; Gametogenesis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6554.pdf |
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| Suquet, Marc; Normant, Yvon; Gaignon, Jean-louis; Quemener, Loic; Fauvel, Christian. |
| The effects of water temperature on pollack (Pollachius pollachius) spawning features were individually assessed. During the spawning period, nine fish sets, each composed of one female and two males (mean body weight: 2.5+/-1.0 kg), were placed in small-volume tanks (2 m(3)). Fish sets were held at 8, 10, or 12 degreesC (n=3 for each temperature). Three females released eggs at 8 and 10 degreesC, while only two spawned at 12 degreesC. One hundred thirty-one individual spawns were collected from the eight spawning fish sets. The number of spawns collected per female was significantly lower at 12 degreesC (3.0+/-3.6), compared to 10 degreesC (17.3+/-10.1) and 8 degreesC (23.3+/-2.5). Egg number (eggs kg(-1) BW) was significantly lowered at 12 degreesC... |
| Tipo: Text |
Palavras-chave: Egg quality; Temperature; Reproduction; Pollachius pollachius; Pollack; Gadoids. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-470.pdf |
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| Rouxel, Catherine; Suquet, Marc; Cosson, J; Severe, Armelle; Quemener, Loic; Fauvel, Christian. |
| The biology of cod reproduction is well described in the scientific literature. However, sperm biology and spermatozoa management are poorly studied in this species. Because of its recent farming expansion, a better knowledge of cod gametes is becoming especially useful. This work aimed at establishing tools to study sperm biology in cod, and also investigated the existence of changes in cod sperm quality during the spawning period. We showed that sperm concentration could be assessed using spectrophotometry at 260 nm. Sperm motility significantly decreased after a 168-h storage at 4 degrees C. A 1:9 dilution of sperm in a non-activating medium (1/3 seawater and 2/3 freshwater, osmotic pressure: 360 mOsm kg(-1)) improved sperm storage. Sperm concentration,... |
| Tipo: Text |
Palavras-chave: Spermatozoa; Quality; Ageing; Sperm; Cod; Gadus morhua. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3829.pdf |
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| Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno. |
| A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied.... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf |
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| Cosson, Jacky; Groison, Anne-laure; Suquet, Marc; Fauvel, Christian. |
| As in many marine fish, motility is triggered by osmotic pressure (OP) in cod and hake spermatozoa and motility ranges several minutes with a high velocity (above 50 mu m/s) period restricted to 100 s. A decrease in the flagellar beat frequency (BF) is partly responsible of this briefness. Detailed observations of the flagella during the motility period showed: 1) sea water provokes OP damages (blebs) which impair the correct wave propagation 2) waves become restricted to the proximal flagellum and tip becomes devoid of wave 3) the wave amplitude decreases. The combination of these factors drastically limits to the earliest period of motility the ability for spermatozoa to efficiently progress towards egg for fertilization. |
| Tipo: Text |
Palavras-chave: Osmotic pressure; Sperm motility; Spermatozoa; Hake; Cod. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6552.pdf |
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| Suquet, Marc; Cosson, J.; Donval, Anne; Labbe, C.; Boulais, Myrina; Haffray, Pierrick; Bernard, Ismael; Fauvel, Christian. |
| This work assesses the present knowledge on Pacific oyster sperm biology in comparison to two marine fish species (turbot and seabass) whose sperm characteristics are well described. Sperm morphology mainly differs by the presence of an acrosome in Pacific oyster which is absent in both fish species. In turbot as in Pacific oyster, a sperm maturation process along the genital tract is observed. Sperm motility is triggered by changes in osmolality for seabass and turbot and in pH for Pacific oyster. However, complementary factors are involved to maintain sperm immotile in the genital tract. Sperm movement duration is very long in Pacific oyster (2024 h), compared to turbot (35 min) and seabass (4050 s). A high capacity of ATP regeneration is observed in... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00111/22220/20059.pdf |
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| Zarski, Daniel; Thaovi Nguyen,; Le Cam, Aurelie; Montfort, Jerome; Dutto, Gilbert; Vidal, Marie-odile; Fauvel, Christian; Bobe, Julien. |
| Variable and low egg quality is a major limiting factor for the development of efficient aquaculture production. This stems from limited knowledge on the mechanisms underlying egg quality in cultured fish. Molecular analyses, such as transcriptomic studies, are valuable tools to identify the most important processes modulating egg quality. However, very few studies have been devoted to this aspect so far. Within this study, the microarray-based transcriptomic analysis of eggs (of different quality) of sea bass (Dicentrarchus labrax) was performed. An Agilent oligo microarray experiment was performed on labelled mRNA extracted from 16 batches of eggs (each batch obtained from a different female) of sea bass, in which over 24,000 published probe arrays were... |
| Tipo: Text |
Palavras-chave: Microarray; Genomics; Transcriptomics; Aquaculture; Controlled reproduction. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00371/48220/48345.pdf |
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| Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian. |
| Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol. Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf |
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| Registros recuperados: 63 | |
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