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| Registros recuperados: 91 | |
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| Darius, Ht; Viallon, J; Gatti, Cm; Chinain, M; Roué, M; Sibat, Manoella; Amzil, Zouher; Hess, Philipp; Vandersea, Mw; Litaker, Rw; Tester, Pa. |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00450/56181/57729.pdf |
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| Mondeguer, Florence; Souard, Florence; Guitton, Yann; Latimier, Marie; Quere, Julien; Sibat, Manoella; Hess, Philipp; Siano, Raffaele. |
| Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a untargeted metabolomics approach by LC-HRMS to study the possible... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00440/55199/56664.pdf |
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| Tong, Mengmeng; Kulis, David M.; Fux, Elie; Smith, Juliette L.; Hess, Philipp; Zhou, Qixing; Anderson, Donald M.. |
| For many years, the study of toxic Dinophysis species was primarily restricted to field populations until it was recently demonstrated that some of these organisms can be mixotrophically cultured in the laboratory with the ciliate prey, Myrionecta rubra. which had previously been fed with cryptophytes of the genus Teleaulax and Geminigera. Here we investigated the influence of growth phase and light intensity on the production of diarrhetic shellfish poisoning (DSP) toxins and pectenotoxins (PTXs) in cultures of Dinophysis acuminata from the northeastern United States. The cell toxin content of okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), and the okadaic acid diol ester (OA-D8) varied significantly with growth phase under all light... |
| Tipo: Text |
Palavras-chave: Diarrhetic shellfish poisoning (DSP); Dinophysis acuminata; Dinophysistoxin (DTX); Growth phase; Light; Okadaic acid (OA); Pectenotoxins (PTXs). |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00037/14827/12195.pdf |
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| Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille. |
| The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cell–based assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tripneustes gratilla; Sea urchin; Echinoidea; Gambierdiscus polynesiensis; Windowscreens; Artificial substrates; QPCR assays; CBA-N2a; LC-MS/MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00436/54716/56153.pdf |
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| Poyer, Salomé; Loutelier-bourhis, Corinne; Mondeguer, Florence; Coadou, Gaël; Hubert-roux, Marie; Enche, Julien; Bossee, Anne; Hess, Philipp; Afonso, Carlos. |
| Saxitoxin and its analogues also called paralytic shellfish poisons (PSPs) are very potent neurotoxins [1] produced by dinoflagellates and referenced as chemical weapon in the chemical warfare convention (CWC). The official detection methods for these toxins present limitations concerning their speed and reliability [2]. Due to the presence of isomers, not differentiable by mass spectrometry (MS), an upstream separation is necessary. In order to separate saxitoxin analogues, hydrophilic interaction liquid chromatography (HILIC) and ion mobility (IM) were used. Those techniques respectively developed for high polar compounds and three dimensional structure differentiation are particularly well adapted to the separation of PSPs. This HILIC/IM-MS coupling... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00179/29039/27475.pdf |
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| Poyer, Salome; Loutelier-bourhis, Corinne; Coadou, Gael; Mondeguer, Florence; Enche, Julien; Bossee, Anne; Hess, Philipp; Afonso, Carlos. |
| The aim of this work was to develop a reliable and efficient analytical method to characterise and differentiate saxitoxin analogues (STX), including sulphated (gonyautoxins, GTX) and non-sulphated analogues. For this purpose, hydrophilic interaction liquid chromatography (HILIC) was used to separate sulphated analogues. We also resorted to ion mobility spectrometry to differentiate the STX analogues because this technique adds a new dimension of separation based on ion gas phase conformation. Positive and negative ionisation modes were used for gonyautoxins while positive ionisation mode was used for non-sulphated analogues. Subsequently, the coupling of these three complementary techniques, HILIC-IM-MS, permitted the separation and identification of STX... |
| Tipo: Text |
Palavras-chave: Saxitoxin; Ion mobility-mass spectrometry; Isomers; Multidimensional separation; HILIC. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00249/36067/34924.pdf |
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| Landrigan, Philip J.; Stegeman, John J.; Fleming, Lora E.; Allemand, Denis; Anderson, Donald M.; Backer, Lorraine C.; Brucker-davis, Françoise; Chevalier, Nicolas; Corra, Lilian; Czerucka, Dorota; Bottein, Marie-yasmine Dechraoui; Demeneix, Barbara; Depledge, Michael; Deheyn, Dimitri D.; Dorman, Charles J.; Fénichel, Patrick; Fisher, Samantha; Gaill, Françoise; Galgani, Francois; Gaze, William H.; Giuliano, Laura; Grandjean, Philippe; Hahn, Mark E.; Hamdoun, Amro; Hess, Philipp; Judson, Bret; Laborde, Amalia; Mcglade, Jacqueline; Mu, Jenna; Mustapha, Adetoun; Neira, Maria; Noble, Rachel T.; Pedrotti, Maria Luiza; Reddy, Christopher; Rocklöv, Joacim; Scharler, Ursula M.; Shanmugam, Hariharan; Taghian, Gabriella; Van De Water, Jeroen A. J. M.; Vezzulli, Luigi; Weihe, Pál; Zeka, Ariana; Raps, Hervé; Rampal, Patrick. |
| Background: Pollution – unwanted waste released to air, water, and land by human activity – is the largest environmental cause of disease in the world today. It is responsible for an estimated nine million premature deaths per year, enormous economic losses, erosion of human capital, and degradation of ecosystems. Ocean pollution is an important, but insufficiently recognized and inadequately controlled component of global pollution. It poses serious threats to human health and well-being. The nature and magnitude of these impacts are only beginning to be understood. Goals: (1) Broadly examine the known and potential impacts of ocean pollution on human health. (2) Inform policy makers, government leaders, international organizations, civil society, and the... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77369/78974.pdf |
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| Longo, Sébastien; Sibat, Manoella; Taiana Darius, Hélène; Hess, Philipp; Chinain, Mireille. |
| Ciguatera poisoning is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. Ciguatera outbreaks are expected to increase worldwide with global change, in particular as a function of its main drivers, including changes in sea surface temperature, acidification, and coastal eutrophication. In French Polynesia, G. polynesiensis is regarded as the dominant source of CTXs entering the food web. The effects of pH (8.4, 8.2, and 7.9), Nitrogen:Phosphorus ratios (24N:1P vs. 48N:1P), and nitrogen source (nitrates vs. urea) on growth rate, biomass, CTX levels, and profiles were examined in four clones of G. polynesiensis at different culture age (D10, D21,... |
| Tipo: Text |
Palavras-chave: Gambierdiscus polynesiensis; French Polynesia; Ciguatera; Ciguatoxins; LC-MS; MS; Toxin profile; Nitrate; Urea; Culture medium acidification. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77382/78981.pdf |
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| Zendong, Suzie Zita; Sibat, Manoella; Herrenknecht, Christine; Hess, Philipp; Mccarron, Pearse. |
| Rationale Accurate quantitative analysis of lipophilic toxins by liquid chromatography-mass spectrometry (LC-MS) requires calibration solution reference materials (RMs) for individual toxin analogs. Untargeted analysis is aimed at identifying a vast number of compounds and thus validation of fully quantitative untargeted methods is not feasible. However, a semi-quantitative approach allowing for profiling is still required and will be strengthened by knowledge of the relative molar response (RMR) of analogs in liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization (ESI). Methods RMR factors were evaluated for toxins from the okadaic acid (OA/DTXs), yessotoxin (YTX), pectenotoxin (PTX), azaspiracid (AZA) and cyclic imine (CI) toxin... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00387/49877/50434.pdf |
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| Reveillon, Damien; Sechet, Veronique; Hess, Philipp; Amzil, Zouher. |
| The neurotoxin β-N-methylamino-L-alanine (BMAA) is naturally present in some microalgal species in the marine environment. The accumulation of BMAA has widely been observed in filter-feeding bivalves that are known to consume primary producers constituting the base of complex aquatic food webs. This study was performed to assess the occurrence of BMAA and isomers in mollusks collected from nine representative shellfish production areas located on the three French coasts (Channel, Atlantic and Mediterranean sites). The use of a highly selective and sensitive HILIC-MS/MS method, with D5DAB as internal standard, revealed the systematic detection of BMAA and DAB, in concentrations ranging from 0.20 to 6.7 μg g-1 dry weight of digestive gland tissues of... |
| Tipo: Text |
Palavras-chave: BMAA; Chaetoceros; Thalassiosira; Phaeodactylum; Bivalves; Seafood. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00297/40780/39784.pdf |
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| Hess, Philipp; Villacorte, Loreen O; Dixon, Mike B; Boerlage, Siobhan Fe; Anderson, Donald M; Kennedy, Maria D; Schippers, Jan C. |
| Once harmful algal blooms (HABs) reach a desalination plant, they can cause significant operational issues and potential health concerns for consumers. These issues stem from two factors – first, the algal cells produce organic matter that can cause filter clogging and membrane fouling, and secondly, some cells produce toxic substances or taste and odor compounds. This chapter first explains the mechanisms for cellular release of organic matter, the types of matter that are produced, and the relative contribution of each type of matter to fouling mechanisms. It then describes the wide range of toxins that are produced by HABs, their mode of toxicity, and analytical methods for detecting them. While taste and odor compounds are non-toxic, they are included... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00407/51837/52438.pdf |
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| Mccarron, Pearse; Emteborg, Hakan; Nulty, Ciara; Rundberget, Thomas; Loader, Jared I.; Teipel, Katharina; Miles, Christopher O.; Quilliam, Michael A.; Hess, Philipp. |
| The development of multi-analyte methods for lipophilic shellfish toxins based on liquid chromatography-mass spectrometry permits rapid screening and analysis of samples for a wide variety of toxins in a single run. Validated methods and appropriate certified reference materials (CRMs) are required to ensure accuracy of results. CRMs are essential for accurate instrument calibration, for assessing the complete analytical method from sample extraction to data analysis and for verifying trueness. However, CRMs have hitherto only been available for single toxin groups. Production of a CRM containing six major toxin groups was achieved through an international collaboration. Preparation of this material, CRM-FDMT1, drew on information from earlier studies as... |
| Tipo: Text |
Palavras-chave: CRM-FDMT1; Certified reference material; Shellfish toxins; Phycotoxins; Accuracy; Precision; Liquid chromatography-mass spectrometry. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00037/14826/12237.pdf |
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| Pisapia, Francesco; Sibat, Manoella; Herrenknecht, Christine; Lhaute, Korian; Gaiani, Greta; Ferron, Pierre-jean; Fessard, Valerie; Fraga, Santiago; Nascimento, Silvia M.; Litaker, R. Wayne; Holland, William C.; Roullier, Catherine; Hess, Philipp. |
| Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from... |
| Tipo: Text |
Palavras-chave: Gambierdiscus excentricus; Maitotoxin-4; Bioguided fractionation; Size-exclusion chromatography (LH-20); Neuro-2a (N2a) assay; LC-HRMS/MS (Q-Tof 6550); LC-LRMS/MS (API4000 QTrap). |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00392/50320/51001.pdf |
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| Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Masseret, Estelle; Hess, Philipp; Amzil, Zouher. |
| The neurotoxin BMAA (β-N-methylamino-l-alanine) and its isomer DAB (2,4-diaminobutyric acid) have been detected in seafood worldwide, including in Thau lagoon (French Mediterranean Sea). A cluster of amyotrophic lateral sclerosis (ALS), a neurodegenerative disease associated with BMAA, has also been observed in this region. Mussels, periphyton (i.e. biofilms attached to mussels) and plankton were sampled between July 2013 and October 2014, and analyzed using HILIC-MS/MS. BMAA, DAB and AEG (N-(2-aminoethyl)glycine) were found in almost all the samples of the lagoon. BMAA and DAB were present at 0.58 and 0.83, 2.6 and 3.3, 4.0 and 7.2 μg g−1 dry weight in plankton collected with nets, periphyton and mussels, respectively. Synechococcus sp., Ostreococcus... |
| Tipo: Text |
Palavras-chave: BMAA; Diatoms; Chaetoceros sp.; Seston; Mytilus galloprovincialis; HILIC-MS/MS. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38607/37192.pdf |
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| Mondeguer, Florence; Souard, Florence; Guitton, Yann; Allard, Pierre-marie; Elie, Nicolas; Sibat, Manoella; Latimier, Marie; Quere, Julien; Wolfender, Jean-luc; Siano, Raffaele; Hess, Philipp. |
| Aquatic ecosystems worldwide have been negatively affected by eutrophication, many of them driven by increasing nutrient inputs from untreated domestic sewage and industrial and agricultural wastewater. During their life cycle, Alexandrium minutum and Scrippsiella donghaienis can produce resistant and revivable cysts that can accumulate in the sediments of the coastal marine environment for hundreds of years. As a working hypothesis, we presumed that these two genera have preserved their adaptation to such ecosystem changes in the form of cysts in the old sediments. From revivified cysts, some cultures of toxic and non-toxic dinoflagellates (A. minutum and S. donghaienis) were analyzed by a metabolomic approach to study the possible adaptive responses of... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00464/57591/59769.pdf |
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| Roué, Mélanie; Smith, Kirsty F.; Sibat, Manoella; Viallon, Jérôme; Henry, Kévin; Ung, André; Biessy, Laura; Hess, Philipp; Darius, Hélène Taiana; Chinain, Mireille. |
| Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed... |
| Tipo: Text |
Palavras-chave: Ciguatera monitoring; Gambierdiscus; Ciguatoxins; SPATT passive samplers; HP20 resin; CBA-N2a; LC-MS; MS; WS artificial substrate; QPCR; HTS metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74143/73670.pdf |
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| Mondeguer, Florence; Antignac, Jean-philippe; Guitton, Yann; Monteau, Fabrice; Le Borgne, Sabrina; Hess, Philipp. |
| The bioactivity of phytoplankton toxins that accumulate in shellfish is almost always tested on animal model (mice assay). In spite of these advantages, the ability of this test to explain the nature of the bioactivity remains limited. Besides, the current sanitary control based on targeted methods of identification and quantification (LC-MS/MS) of known toxins, do not permit to detect unknown toxins. In order to cope with this need of identification of unknown toxic substances, a next approach based on global and differential metabolomic profiling was proposed. The first results obtained from extracts that have shown a positive toxicity in mice without the substances potentially responsible for these toxic effects have been measured by targeted methods,... |
| Tipo: Text |
Palavras-chave: Métabolomique; LC-HRMS; Biotoxines marines; Biomarqueur. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00077/18784/16352.pdf |
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| Mondeguer, Florence; Sibat, Manoella; Reubrecht, Sébastien; Amzil, Zouher; Bormans, Myriam; Hess, Philipp; Briand, Enora. |
| Cyanobacterial proliferation is one of the most harmful hazards, in both freshwater and marine ecosystems. Cyanobacteria are well known for their ability to produce a wide variety of bioactive compounds, some of which have been described as allelochemicals. There is growing evidence that these secondary metabolites play an important role in shaping community composition through biotic interactions; however, for the most part, their biological role and mode of regulation of the production are poorly understood. In temperate eutrophic freshwaters, Microcystis and Planktothrix often co-occur, with Planktothrix being an early colonizer and Microcystis appearing subsequently. By integrating LC-MS/MS molecular networking and an innovative experimental design, we... |
| Tipo: Text |
Palavras-chave: Cyanobacteria; Cyanotoxins; Plankton interactions; Co_culture; LC_HRMS; Molecular Network; Metabolomics. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00405/51666/52215.pdf |
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| Briand, Enora; Reubrecht, Sébastien; Mondeguer, Florence; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Bormans, Myriam. |
| Freshwater cyanobacteria are known for their ability to produce bioactive compounds, some of which have been described as allelochemicals. Using a combined approach of co‐cultures and analyses of metabolic profiles, we investigated chemically mediated interactions between two cyanobacterial strains, Microcystis aeruginosa PCC7806 and Planktothrix agardhii PCC7805. More precisely, we evaluated changes in growth, morphology and metabolite production and release by both interacting species. Co‐culture of Microcystis with Planktothrix resulted in a reduction of the growth of Planktothrix together with a decrease of its trichome size and alterations in the morphology of its cells. The production of intracellular compounds by Planktothrix showed a slight... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00470/58152/60632.pdf |
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| Zendong, Suzie Zita; Herrenknecht, Christine; Mondeguer, Florence; Hess, Philipp. |
| By 2014, the new targeted LC-MS/MS reference method for the detection of lipophilic toxins will replace the mouse bioassay (MBA). This bioassay, which has the advantage of being a rapid and global toxicity test, can not be used for toxin identification purposes. Furthermore, it has appeared that, in some cases, the mouse bioassay could reveal uncharacterized toxicities that could not be elucidated by targeted mass spectrometry methods. Therefore, moving from the global toxicity assessment test to a targeted technique leads to a lack of information on emerging toxins. Objectives: - Beside targeted methods, develop metabolomic approaches to screen for known and unknown emerging toxins - Develop passive sampling devices suitable for toxins of varying... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00179/29040/27478.pdf |
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| Registros recuperados: 91 | |
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