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| Registros recuperados: 97 | |
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| Miossec, Laurence; Le Guyader, Soizick; Haugarreau, Larissa; Comps, Marie-annick; Pommepuy, Monique. |
| Several outbreaks of gastroenteritis related to the consumption of shellfish (frequently eaten raw) have been reported in different parts of the world. In Europe, hum an calicivirus infections may have been involved in winter outbreaks in recent years, although there is little evidence confirming such viral contamination in shellfish. This study presents the first results of a field survey on viral contamination in two shelllish harvesting areas along the French Mediterranean coast. The first, consisting mainly of oyster beds, was c1assified in category A, as determined by fecal coliform counts in shellfish (European Community Directive 91/492), and the second, a mussel bed, was c1assified in category C. Shelllish samples were collected monthly between... |
| Tipo: Text |
Palavras-chave: Shellfish; Viral contamination; Enterovirus; Human calicivirus; Rotavirus; Astrovirus; Fecal coliforms; Gastroenteritis. |
| Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/00001/11257/7802.pdf |
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| Boukerb, Amine Mohamed; Schaeffer, Julien; Serghine, Joelle; Carrier, Gregory; Le Guyader, Soizick; Gourmelon, Michele. |
| As determined by a hybrid approach combining Oxford Nanopore MinION and Illumina MiniSeq sequence data, Campylobacter armoricus strain CA639 harbored a circular chromosome of 1,688,169 bp with a G+C content of 28.47% and two plasmids named pCA639-1 and pCA639-2, with lengths of 51,123 and 28,139 bp, and G+C contents of 26.5% and 28.45%, respectively. |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00600/71164/69495.pdf |
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| Garry, Pascal; Hervio Heath, Dominique; Le Guyader, Soizick. |
| Ce rapport présente une synthèse des actions, travaux menés par l’équipe, accompagnée par des stagiaires, doctorants, post doctorants qu’il est important de remercier pour leur contribution. Cette année 2016 a de nouveau été une année riche en résultats innovants qui permet à notre équipe d’être reconnue au niveau national et international. En effet la Microbiologie sanitaire, thème de recherche fédérant notre laboratoire, thématique à l’interface de la santé publique, de la qualité des eaux côtières et des coquillages, nous permet de développer nos activités sur le terrain ou sur une approche plus fondamentale. Les résultats obtenus nous permettent d’exercer notre activité de Laboratoire National de Référence, en apportant connaissance scientifique et... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00433/54493/55858.pdf |
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| Le Guyader, Soizick; Garry, Pascal; Ollivier, Joanna; Piquet, Jean-come. |
| The shellfish safety has long been considered a public health problem. The implementation of a regulation based on a bacterial indicator of faecal contamination has significantly reduced outbreaks of bacterial origin. However, epidemics of viral gastroenteritis due to the presence of noroviruses from human sewage persist. We demonstrated few years ago that oysters are not just a passive filter but are able to select some viral strains, via the presence of specific ligands, very similar to ligands observed in humans for these same virus. Ongoing developments, in conjunction with the application of metagenomic tools, will allow us to better understand the fate of some human pathogens after their release into the coastal environment. |
| Tipo: Text |
Palavras-chave: Shellfish; Microbiology; Oysters; Norovirus. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00599/71141/69457.pdf |
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| Strubbia, Sofia; Schaeffer, Julien; Oude Munnink, Bas B.; Besnard, Alban; Phan, My V. T.; Nieuwenhuijse, David F.; De Graaf, Miranda; Schapendonk, Claudia M. E.; Wacrenier, Candice; Cotten, Matthew; Koopmans, Marion P. G.; Le Guyader, Soizick. |
| Metagenomic sequencing is a promising method to determine the virus diversity in environmental samples such as sewage or shellfish. However, to identify the short RNA genomes of human enteric viruses among the large diversity of nucleic acids present in such complex matrices, method optimization is still needed. This work presents methodological developments focused on norovirus, a small ssRNA non-enveloped virus known as the major cause of human gastroenteritis worldwide and frequently present in human excreta and sewage. Different elution protocols were applied and Illumina MiSeq technology were used to study norovirus diversity. A double approach, agnostic deep sequencing and a capture-based approach (VirCapSeq-VERT) was used to identify norovirus in... |
| Tipo: Text |
Palavras-chave: Norovirus; Sewage; Oysters; Metagenomic sequencing; Metavirome. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00586/69765/67656.pdf |
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| Desdouits, Marion; Wacrenier, Candice; Ollivier, Joanna; Schaeffer, Julien; Le Guyader, Soizick. |
| Human noroviruses (NoV) cause epidemics of acute gastroenteritis (AGE) worldwide and can be transmitted through consumption of contaminated foods. Fresh products such as shellfish can be contaminated by human sewage during production, which results in the presence of multiple virus strains, at very low concentrations. Here, we tested a targeted metagenomics approach by deep-sequencing PCR amplicons of the capsid (VP1) and polymerase (RdRp) viral genes, on a set of artificial samples and on shellfish samples associated to AGE outbreaks, to evaluate its advantages and limitations in the identification of strains from the NoV genogroup (G) II. Using artificial samples, the method allowed the sequencing of most strains, but not all, and displayed variability... |
| Tipo: Text |
Palavras-chave: Norovirus; Foodborne virus; Metagenomics; Amplicon deep sequencing; Viral diversity. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00646/75831/76818.pdf |
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| Garry, Pascal; Le Guyader, Soizick. |
| Ce rapport présente une synthèse des actions, travaux menés par l’équipe, accompagnée par des stagiaires, doctorants, post doctorants qu’il est important de remercier pour leur contribution. Cette année 2017 a de nouveau été une année riche en résultats innovants qui permet à notre équipe d’être reconnue au niveau national et international. En effet la Microbiologie sanitaire, thème de recherche fédérant notre laboratoire, thématique à l’interface de la santé publique, de la qualité des eaux côtières et des coquillages, nous permet de développer nos activités sur le terrain ou sur une approche plus fondamentale. Les résultats obtenus nous permettent d’exercer notre activité de Laboratoire National de Référence, en apportant connaissance scientifique et... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00465/57686/59887.pdf |
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| Le Saux, Jean-claude; Hervio Heath, Dominique; Pommepuy, Monique; Le Guyader, Soizick. |
| En 2006, le nombre d'informations recensées par Ifremer, relatives aux toxi-infections alimentaires collectives (TIAC) liées à la consommation de coquillages vivants en France a triplé par rapport aux années précédentes, avec 45 foyers suspectés ou confirmés pour environ 400 cas estimés. Pendant cette même période, les alertes européennes sont restées au même niveau que les années antérieures avec 22 notifications communautaires. Majoritairement, ces TIAC ont été déclarées au cours du premier trimestre de l'année avec comme vecteur alimentaire incriminé principalement les huîtres et comme agent causal suspecté ou identifié les norovirus et un virus émergent, le virus Aichi. Sur l'ensemble de ces TIAC, seul un nombre très limité d'entre elles a pu faire... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00306/41708/40919.pdf |
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| Le Guyader, Soizick; Haugarreau, Larissa; Miossec, Laurence; Dubois, Eric; Pommepuy, Monique. |
| The main pathogenic enteric viruses able to persist in the environment, such as hepatitis A virus (HAV), Norwalk-like virus (NLV), enterovirus (EV), rotavirus (RV), and astrovirus (AV), were detected by reverse transcription-PCR and hybridization in shellfish during a 3-year study. Oyster samples (n = 108), occasionally containing bacteria, were less frequently contaminated, showing positivity for AV (17%), NLV (23%), EV (19%), and RV (27%), whereas mussel samples, collected in areas routinely impacted by human sewage, were more highly contaminated: AV (50%), HAV (13%), NLV (35%), EV (45%), and RV (52%). Sequences obtained from HAV and NLV amplicons shelved a great variety of strains, especially for NLV (strains close to Mexico, Snow Mountain Agent, or... |
| Tipo: Text |
Palavras-chave: Molecular technique; Seasonal variation; Human pathology; Viral contamination; Shellfish; Enteric virus. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-1363.pdf |
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| Registros recuperados: 97 | |
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