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| Registros recuperados: 48 | |
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| Treguier, Cathy; Schaeffer, Julien; Le Saux, Jean-claude; Cochennec-laureau, Nathalie; Le Guyader, Soizick. |
| Les norovirus (NoV) sont responsables des épidémies hivernales de gastroentérites aiguës dans la population. Ils sont excrétés dans les fèces humains, puis se retrouvent, après épuration insuffisante, dans le milieu marin naturel où ils peuvent contaminer des coquillages, qui à leur tour risquent de déclencher des gastroentérites chez les consommateurs. Cette étude vise à améliorer les connaissances sur la contamination des coquillages en élevage en rivière d’Auray par les NoV, en lien avec les bactéries témoins de contamination fécale Escherichia coli (E. coli). L’objectif est de suivre les flux de NoV et d’E. coli apportés par différents rejets (dont une station d’épuration) impactant la zone conchylicole et leur influence sur la qualité des coquillages... |
| Tipo: Text |
Palavras-chave: Norovirus; Coquillages; Contamination; Station d’épuration; Flux; Rivière d’Auray; Escherichia coli.. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00267/37778/35829.pdf |
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| Le Guyader, Soizick; Le Saux, Jean-claude; Ambert-balay, Katia; Krol, Joanna; Serais, Ophelie; Parnaudeau, Sylvain; Giraudon, Helene; Delmas, Gilles; Pommepuy, Monique; Pothier, Pierre; Atmar, Robert L.. |
| Following a flooding event close to a shellfish production lagoon, 205 cases of gastroenteritis were linked to oyster consumption. Twelve stool samples from different individuals were collected. Analysis showed that eight samples were positive for multiple enteric viruses, and one stool sample had seven different enteric viruses. Analysis of shellfish implicated in the outbreak allowed detection of the same diversity of enteric viruses, with some viral genomic sequences being identical to those obtained from stool sample analysis. Shellfish were contaminated by as many as five different enteric viruses. For the first time in Europe, Aichi virus was identified in oyster samples. Shellfish samples collected over 3 weeks following the outbreak showed a... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6099.pdf |
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| Pommepuy, Monique; Hervio Heath, Dominique; Caprais, Marie-paule; Gourmelon, Michele; Le Saux, Jean-claude; Le Guyader, Soizick. |
| Introduction : "The occurrence of pathogenic microorganisms in seawater or in shellfish could exist anytime sewage from human or animal origin would be discharged to the coast" (Metcalf, 1982). According to the diseases occurring in the human population or in animals, pathogens might be present in recreational waters or in shellfish. Thus, the presence of human enteric viruses (norovirus, astrovirus, rotavirus, hepatitisAvirus (HAV)) and pathogenic bacteria (Salmonella, Listeria monocytogenes, Shiga-toxin-producing Escherichia coli (STEC), Vibrio cholerae, Vibrio parahaemolyticus, etc.) has been reported in coastal areas for a long time (Colwell, 1978; Metcalf, 1978; Melnick et al., 1979; Grimes, 1991; Bosch et al., 2001;Kong et al., 2002). These... |
| Tipo: Text |
Palavras-chave: Estimating model; Infection dose; Microorganisms; STEC; Bacteria; Virus; Fecal contamination; Pathogenic agent; Marine sediment; Estuarine water; Coastal area; Marine water. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1207.pdf |
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| Guillois-becel, Y.; Couturier, E.; Le Saux, Jean-claude; Roque-afonso, A. M.; Le Guyader, Soizick; Le Goas, A.; Pernes, J.; Le Bechec, S.; Briand, A.; Robert, C.; Dussaix, E.; Pommepuy, Monique; Vaillant, V.. |
| Following the notification of nine hepatitis A cases clustered in the Cotes d'Armor district in northwestern France, epidemiological, environmental and microbiological investigations were set up in order to identify the source and vehicle of contamination and implement control measures. In total, 111 cases were identified in the outbreak, all of whom lived or had stayed as tourists in the Cotes d'Armor district. Of the cases, 87% had eaten raw shellfish, and 81% specifically oysters. Traceback investigations carried out on raw shellfish consumed by the cases showed that the raw shellfish originated from a single shellfish farm. The shellfish were probably contaminated either in the submersible tanks or in a depuration land-based tank where they were... |
| Tipo: Text |
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| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6685.pdf |
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| Le Guyader, Soizick; Krol, Joanna; Ambert-balay, Katia; Ruvoen-clouet, Nathalie; Desaubliaux, Benedicte; Parnaudeau, Sylvain; Le Saux, Jean-claude; Ponge, Agnes; Pothier, Pierre; Atmar, Robert L.; Le Pendu, Jacques. |
| Noroviruses have been recognized to be the predominant agents of nonbacterial gastroenteritis outbreaks in humans, and their transmission via contaminated shellfish consumption has been demonstrated. Norovirus laboratory experiments, volunteer challenge studies, and community gastroenteritis outbreak investigations have identified human genetic susceptibility factors related to histo-blood group antigen expression. Following a banquet in Brittany, France, in February 2008, gastroenteritis cases were linked to oyster consumption. This study identified an association of the norovirus illnesses with histo-blood group expression, and oyster contamination with norovirus was confirmed by qualitative and quantitative analyses. The secretor phenotype was... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11309/8050.pdf |
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| Da Silva, A; Le Saux, Jean-claude; Parnaudeau, Sylvain; Pommepuy, Monique; Elimelech, M; Le Guyader, Soizick. |
| Noroviruses, an important cause of gastroenteritis, are excreted by infected individuals and are therefore present in wastewater. We quantified norovirus genogroup I (GI) and GII in wastewater at different locations in France and evaluated removal by a range of treatment types, including basic (waste stabilization pond), current industry standard (activated sludge), and state-of-the-art (submerged membrane bioreactor) treatments. Noroviruses were quantified using real-time reverse transcription-PCR (rRT-PCR). Mengovirus was used as a virus extraction control, and internal controls were used to verify the level of GI and GII rRT-PCR inhibition. A total of 161 (81 influent and 79 effluent) samples were examined; GI and GII were detected in 43 and 88% of the... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3571.pdf |
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| Pommepuy, Monique; Caprais, Marie-paule; Le Saux, Jean-claude; Le Mennec, Cecile; Parnaudeau, Sylvain; Madec, Yvon; Monier, Martial; Brest, Goulven; Le Guyader, Francoise. |
| Depuration processes try to eliminate microorganisms using seawater to allow living, filter-feeding shellfish to naturally purge themselves from agents they accumulated from the environment. Until now the only parameter to evaluate depuration was the decrease in E. coli counts. Studies have shown that viruses can persist in the environment longer than E. coli and that shellfish meeting the endstandard (< 230 E.colil /100g) have been involved in viral outbreaks. Starting in February 2000, the European Commission has been funding (Fifth Framework Programme), a 3-year project entitled "Virus Safe Seafood". One of the specific objectives of this project is to innovate technology for shellfish depuration. For this purpose a depuration system was designed and... |
| Tipo: Text |
Palavras-chave: Bacteriophage FRNA; Depuration; Shellfish. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/acte-1208.pdf |
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| Guillois-bécel, Y.; Couturier, E.; Le Saux, Jean-claude; Roque-afonso, A.m.; Le Guyader, Soizick; Le Goas, A.; Pernes, J.; Le Bechec, S.; Briand, A.; Robert, C.; Dussaix, E.; Pommepuy, Monique; Vaillant, C.. |
| Following the notification of nine hepatitis A cases partly clustered near an oyster growing area in the Côtes d’Armor district (Brittany, France), epidemiological, environmental and microbiological investigations were performed in order to identify the source and vehicle of contamination and devise control measures. The investigations identified 111 cases, all of whom lived or had stayed in the district. Of these cases, 87% had eaten raw bivalve molluscs and 81% had specifically eaten oysters. The raw molluscs originated from a single shellfish farm located at the north of Paimpol bay. The shellfish were probably contaminated while being stored in submersible tanks or purified in a land-based tank. They were tainted either by sewage overflows or releases... |
| Tipo: Text |
Palavras-chave: Hepatitis A; Outbreak; Oysters; Epidemiological investigations; Traceback investigations. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00066/17750/15272.pdf |
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| Miura, Takayuki; Schaeffer, Julien; Le Saux, Jean-claude; Le Mehaute, Philippe; Le Guyader, Soizick. |
| We investigated removal of noroviruses, sapoviruses, and rotaviruses in a full-scale membrane bioreactor (MBR) plant by monitoring virus concentrations in wastewater samples during two gastroenteritis seasons and evaluating the adsorption of viruses to mixed liquor suspended solids (MLSS). Sapoviruses and rotaviruses were detected in 25% of MBR effluent samples with log reduction values of 3- and 2-logs in geometric mean concentrations, respectively, while noroviruses were detected in only 6% of the samples. We found that norovirus and sapovirus concentrations in the solid phase of mixed liquor samples were significantly higher than in the liquid phase (P < 0.01, t test), while the concentration of rotaviruses was similar in both phases. The efficiency... |
| Tipo: Text |
Palavras-chave: Norovirus; Sapovirus; Rotavirus; MBR; Mixed liquor suspended solids; Adsorption. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00414/52548/53441.pdf |
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| Le Saux, Jean-claude; Lora, M.; Schaeffer, Julien; Poiraud, G.; Le Guyader, Soizick. |
| L’objet de cette étude est d’évaluer, par les méthodes de détection virale en cours de validation au niveau européen, le niveau de présence des norovirus dans les huîtres à l’expédition et sur les lieux de vente dans un département test, celui de la Vendée. En effet, aujourd’hui peu d’informations existent au niveau international et national sur la prévalence des norovirus dans les coquillages mis dans les circuits de consommation, alors que tous les ans des coquillages, répondant aux critères de mise en marché, sont incriminés ou suspectés dans des foyers de toxi-infections alimentaires collectives liés à la consommation de coquillages, majoritairement contaminés par des virus et plus particulièrement par les norovirus. Cette étude sur la période de... |
| Tipo: Text |
Palavras-chave: Microbiologie; Coquillages; Norovirus; Huître creuse; Détection; Santé publique; Département Vendée. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00185/29650/28019.pdf |
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| Schaeffer, Julien; Treguier, Cathy; Piquet, Jean-come; Gachelin, Sonia; Cochennec-laureau, Nathalie; Le Saux, Jean-claude; Garry, Pascal; Le Guyader, Soizick. |
| As human population increases worldwide, water quality will become increasingly problematic, and food consumed raw may be of higher risk. This is already evident for oysters grown in coastal areas - despite regulations based on bacterial indicators, oysters are still implicated in food-borne outbreaks worldwide. The pathogens most frequently detected are human noroviruses, which are shed at high concentrations in human excreta and are very resistant to environmental conditions. Sewage treatment plants usually apply a variety of steps such as activated sludge treatment, chlorine or UV disinfection to eliminate contaminants, these processes have variable efficacy. This study demonstrates the impact of replacing an old lagoon-based sewage treatment plant with... |
| Tipo: Text |
Palavras-chave: Norovirus; Shellfish safety; Sewage treatment; Coastal water quality. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00450/56140/57707.pdf |
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| Blanchard, Michel; Le Saux, Jean-claude; Piriou, Jean-yves. |
| Un recensement des apports provenant de certains bassins versants dans l'estuaire de l'Aber-Benoît (Bretagne-nord, France) montre que les contaminants susceptibles d'affecter la qualité de l'eau alimentant les huîtres sont de natures diverses. Parmi les éléments étudiés, les mesures donnent des flux minima journaliers de 10(13) coliformes fécaux, 2 tonnes d'azote, 150 kilogrammes de phosphate sur l'estuaire, et 27 grammes de triazines, pesticides les plus importants sur la rivière Aber-Benoît. |
| Tipo: Text |
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| Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00082/19285/16880.pdf |
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| Maalouf, Haifa; Zakhour, Maha; Le Pendu, Jacques; Le Saux, Jean-claude; Atmar, Robert L.; Le Guyader, Francoise S.. |
| Bivalve molluscan shellfish, such as oysters, filter large volumes of water as part of their feeding activities and are able to accumulate and concentrate different types of pathogens, particularly noroviruses, from fecal human pollution. Based on our previous observation of a specific binding of the Norwalk strain (prototype norovirus genogroup I) to the oyster digestive tract through an A-like carbohydrate structure indistinguishable from human blood group A antigen and on the large diversity between strains in terms of carbohydrate-binding specificities, we evaluated the different ligands implicated in attachment to oysters tissues of strains representative of two main genogroups of human norovirus. The GI.1 and GII.4 strains differed in that the latter... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12215/9045.pdf |
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| Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Kopp, Joel; Mouillard, Gilbert; Gerla, Daniel; Le Saux, Jean-claude; Raguenes, Pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Fillon, Alain. |
| The REPAMO network (network ofmollusc pathology), created in 1986, ensures the survey of shellfish health status along French coasts according? to European Directives 91/67/EEC and 95/70/EEC. The aims of the network are 1 - to prevent the introduction and spread of exotic pathogens, 2-to study the means to decrease the impact of pathogens already present and to survey their evolution. In 2003, the REPAMO network focused on the survey oflisted diseases (bonamiosis and marteiliosis) in two areas under agreement process (Banc de Granville and zone X) and on the study of abnormal morlalities. Number of reported abnormal morlalities has increased in 2003. This rise can be partly explained by the recent network restructuring, but also by the summer heatwave and... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00086/19699/17340.pdf |
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| Registros recuperados: 48 | |
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