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| Registros recuperados: 26 | |
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| Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud. |
| Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters... |
| Tipo: Text |
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf |
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| Harney, Ewan; Rastrick, Samuel; Artigaud, Sébastien; Pisapia, Julia; Miner, Philippe; Pichereau, Vianney; Strand, Oivind; Boudry, Pierre; Charrier, Gregory. |
| Elevated atmospheric CO2 is a major driver of global change in the ocean, causing sea surface temperatures to rise and acidification of marine environments. Together these stressors may have additive, synergistic or unforeseen consequences for many marine organisms, particularly during early life stages. The economically important King Scallop (Pecten maximus) is found along a large latitudinal gradient in the Eastern Atlantic, but it is unclear how this species will respond to changing environmental conditions. Studies of genetic structure suggest that Atlantic populations (from Spain to the UK) are genetically distinct from Norwegian populations, a result that is borne out by phenotypic differences. Yet whether genetic differences arise as a result of... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51520/52114.pdf |
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| Genard, Bertrand; Larouche, Olivier; Nicolas, Jean-louis; Miner, Philippe; Beaudin, Marie-lou; Tremblay, Rejean. |
| The aim of this project was to evaluate the impact of probiotic (Phaeobacter gallaeciensis, X34 strain) treatment on the complete development (from veliger to metamorphosis) of Pecten maximus larvae in the context of a bacterial challenge and in conditions more representative of hatchery practices. To that effect, the present study was divided into two main steps. In the first, we used in vitro analyses (antibiograms and microplate assays) to validate the inhibition abilities of X34 on the growth of four Vibrio pathogen species. During the second step, we added pathogens (Vibrio pectenicida) into rearing tanks after two weeks of pre-treatment with the probiotic and then followed the larval development of Pecten maximus through the monitoring of survival... |
| Tipo: Text |
Palavras-chave: Probiotics; Hatchery; Bacterial challenge; Larval development; Pecten maximus; Phaeobacter gallaeciensis; Vibrio. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00213/32429/30895.pdf |
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| Offret, Clement; Paulino, Sauvann; Gauthier, Olivier; Château, Kevin; Bidault, Adeline; Corporeau, Charlotte; Miner, Philippe; Petton, Bruno; Pernet, Fabrice; Fabioux, Caroline; Paillard, Christine; Blay, Gwenaelle Le. |
| Digestive microbiota provides a wide range of beneficial effects on host physiology and are therefore likely to play a key role in marine intertidal bivalve ability to acclimatize to the intertidal zone. This study investigated the effect of intertidal levels on the digestive bacterial microbiota of oysters Crassostrea gigas and clams Ruditapes philippinarum, two bivalves with different ecological niches. Based on the 16S rRNA region sequencing, digestive glands, seawater and sediments harbored specific bacterial communities, dominated by OTUs assigned to the Mycoplasmatales, Desulfobacterales and Rhodobacterales orders, respectively. Field implantation modified digestive bacterial microbiota of both bivalve species according to their intertidal position.... |
| Tipo: Text |
Palavras-chave: Microbiota; Oysters; Clams; Intertidal zone; Digestive gland; Metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/73961/73320.pdf |
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| Robert, Rene; Trut, Gilles; Laborde, Jean-louis; Miner, Philippe. |
| Until 1960, the black scallop Chlamys varia (Linné, 1758) was largely distributed over the French Atlantic coast. Nowadays, most of the deposits are gone. Since controlled mollusc reproduction techniques are now relatively mastered, some reimplatation attempts have been conducted on a number of oyster culture sites, including the Arcachon Bay, from 1989 to 1992. Despite some good results in hatchery and nursery, the rearing attempts failed. If some good growth rates have been monitored in the Arcachon Bay over these three years, these performances have been frustrated by very high mortality rates occurring shortly after the setting up of the farms. Although this species is known to be relatively sensitive to environmental factors, the hydrobiological... |
| Tipo: Text |
Palavras-chave: Bassin Arcachon; Elevage; Chlamys varia; Pétoncle. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-6193.PDF |
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| Delaporte, Maryse; Soudant, Philippe; Moal, Jeanne; Lambert, Christophe; Quere, Claudie; Miner, Philippe; Choquet, Gwenaelle; Paillard, Christine; Samain, Jean-francois. |
| The impact of diets upon the fatty acid composition of haemocyte polar lipids and consequently upon immune parameters has been tested in the oyster Crassostrea gigas and the clam Ruditapes philippinarum. Oysters and clams were fed each of three cultured algae: Chaetoceros calcitrans, which is rich in 20:5(n-3) and 20:4(n-6) and poor in 22:6(n-3) fatty acids; T-Iso (Isochrysis sp.), which is rich in 22:6(n-3) and deficient in 20:5(n-3) and 20:4(n-6); and Tetraselmis suecica, which is deficient in 22:6(n-3) and contains only small amounts of 20:5(n-3) and 20:4(n-6). Fatty acid composition of haemocyte polar lipids was greatly affected by the diet. Oysters and clams fed C. calcitrans maintained a higher proportion of 20:5(n-3) and 20:4(n-6) in their haemocyte... |
| Tipo: Text |
Palavras-chave: Ruditapes philippinarum; Crassostrea gigas; Polyunsaturated fatty acids; Phagocytosis; Oxidative activity; Haemocyte membrane; Nutrition; Immunology; Bivalve; Immunologie; Bivalve; Ruditapes philippinarum; Crassostrea gigas; Acides gras poly insatures; Phagocytose; Flambee respiratoire; Membrane; Hemocyte; Nutrition. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-354.pdf |
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| Registros recuperados: 26 | |
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