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| Registros recuperados: 27 | |
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| Alain, Karine; Pignet, Patricia; Zbinden, M; Quillevere, M; Duchiron, F; Donval, Jean-pierre; Lesongeur, Francoise; Raguenes, Gerard; Crassous, Philippe; Querellou, Joel; Cambon-bonavita, Marie-anne. |
| A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex... |
| Tipo: Text |
Palavras-chave: Caminicella sporogenes; Clostridiales; Thermophile; Deep sea hydrothermal vent. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-528.pdf |
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| Zeng, Xiang; Birrien, Jean-louis; Fouquet, Yves; Cherkashov, Georgy; Jebbar, Mohamed; Querellou, Joel; Oger, Philippe; Cambon-bonavita, Marie-anne; Xiao, Xiang; Prieur, Daniel. |
| A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise 'Serpentine'. At a depth of 4100 m, the site 'Ashadze' is the deepest vent field known so far. Smoker samples were collected with the ROV 'Victor 6000' and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 degrees C and a pressure range of 20 to 120 MPa, with optima for 98 degrees C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic... |
| Tipo: Text |
Palavras-chave: Thermococcales; Extremophile; Deep sea hydrothermal vent; High hydrostatic pressure; Archaea. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6812.pdf |
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| Palud, Adeline; Villani, Giuseppe; L'Haridon, Stephane; Querellou, Joel; Raffin, Jean-paul; Henneke, Ghislaine. |
| Spontaneous and induced abasic sites in hyperthermophiles DNA have long been suspected to occur at high frequency. Here, Pyrococcus abyssi was used as an attractive model to analyse the impact of such lesions onto the maintenance of genome integrity. We demonstrated that endogenous AP sites persist at a slightly higher level in P. abyssi genome compared with Escherichia coli. Then, the two replicative DNA polymerases, PabpolB and PabpolD, were characterized in presence of DNA containing abasic sites. Both Pabpols had abortive DNA synthesis upon encountering AP sites. Under running start conditions, PabpolB could incorporate in front of the damage and even replicate to the full-length oligonucleotides containing a specific AP site, but only when present at... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-6113.pdf |
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| Slobodkina, G. B.; Kolganova, T. V.; Chernyh, N. A.; Querellou, Joel; Bonch-osmolovskaya, E. A.; Slobodkin, A. I.. |
| A thermophilic, anaerobic, chemolithoautotrophic bacterium (designated strain SL50(T)) was isolated from a hydrothermal sample collected at the Mid-Atlantic Ridge from the deepest of the known World ocean hydrothermal fields, Ashadze field (12 degrees 58' 21 '' N 44 degrees 51' 47 '' W) at a depth of 4100 m. Cells of strain SL50(T) were motile, straight to bent rods with one polar flagellum, 0.5-0.6 mu m in width and 3.0-3.5 mu m in length. The temperature range for growth was 25-75 degrees C, with an optimum at 60 degrees C. The pH range for growth was 5.0-7.5, with an optimum at pH 6.5. Growth of strain SL50(T) was observed at NaCl concentrations ranging from 1.0 to 6.0% (w/v) with an optimum at 2.5 % (w/v). The generation time under optimal growth... |
| Tipo: Text |
Palavras-chave: Thermophile; Archaeon. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6598.pdf |
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| Postec, Anne; Lesongeur, Francoise; Pignet, Patricia; Ollivier, B; Querellou, Joel; Godfroy, Anne. |
| The prokaryotic diversity of culturable thermophilic communities of deep-sea hydrothermal chimneys was analysed using a continuous enrichment culture performed in a gas-lift bioreactor, and compared to classical batch enrichment cultures in vials. Cultures were conducted at 60 degrees C and pH 6.5 using a complex medium containing carbohydrates, peptides and sulphur, and inoculated with a sample of a hydrothermal black chimney collected at the Rainbow field, Mid-Atlantic Ridge, at 2,275 m depth. To assess the relevance of both culture methods, bacterial and archaeal diversity was studied using cloning and sequencing, DGGE, and whole-cell hybridisation of 16S rRNA genes. Sequences of heterotrophic microorganisms belonging to the genera Marinitoga,... |
| Tipo: Text |
Palavras-chave: Metabolic interactions; 16S rRNA gene; Thermophiles; Bioreactor; Continuous enrichment cultures; Deep sea hydrothermal vent; Microbial diversity. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3885.pdf |
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| Postec, Anne; Urios, Laurent; Lesongeur, Francoise; Ollivier, Bernard; Querellou, Joel; Godfroy, Anne. |
| The microflora developing during a continuous enrichment culture from a hydrothermal chimney sample was investigated by molecular methods. The culture was performed in a gas-lift bioreactor under anaerobic conditions, at 90 degrees C and pH 6.5, on a complex medium containing sulfur as the terminal electron acceptor. Archaeal and bacterial diversity was studied. Microorganisms affiliated with the genera Pyrococcus, Marinitoga, and Bacillus were detected through DGGE analysis of 16S rDNA. Additional sequences phylogenetically related to Thermococus and epsilon-Proteobacteria were detected by cloning and sequencing of 16S rDNA from two samples of the enrichment culture. In comparison, the sequences retrieved from cloning analysis from an enrichment culture... |
| Tipo: Text |
Palavras-chave: Sequence alignment; Anaerobic digester; Thermotogales; Thermophile; Deep sea vent. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-364.pdf |
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| Ishibashi, Jun-ichiro; Lupton, John E.; Yamaguchi, Toshiyuki; Querellou, Joel; Nunoura, Takuro; Takai, Ken. |
| TheValu Fa Ridge (VFR) in the southern Lau Basin—located behind the westward‐dipping Tonga‐Kerrnadec subduction zone—was one of the earliest targets to b e explored for hydrothermal activity in the southwestern Pacific Ocean (Figure l).In 1989, the French‐German dive program NAUTILAU (Nautile‐lan) discovered active hydrothermal fields venting high‐temperature fluids with high acidity accompanied by massive ore deposits in this area [Fbuquet et al., 1993]. In September–October 2004, the SWEEP VENTS (Southwestern Edge of Pacific hydrothermal vents) expedition explored and sampled the hydrothermal systems of the VFR using the deep submergence research vehicle (DSRV) Shinkai 6500. The 2004 Shinkai dives focused on the geobiological and geochemical character of... |
| Tipo: Text |
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| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/00451/56261/57831.pdf |
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| Registros recuperados: 27 | |
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