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| Registros recuperados: 35 | |
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| Akoumany, Katy; Zykwinska, Agata; Sinquin, Corinne; Marchand, Laetitia; Fanuel, Mathieu; Ropartz, David; Rogniaux, Hélène; Pipelier, Muriel; Delbarre Ladrat, Christine; Colliec Jouault, Sylvia. |
| Bacteria from deep-sea hydrothermal vents constitute an attractive source of bioactive molecules. In particular, exopolysaccharides (EPS) produced by these bacteria become a renewable source of both biocompatible and biodegradable molecules. The low molecular weight (LMW) derivatives of the GY785 EPS produced by the deep-sea hydrothermal vent strain Alteromonas infernus have previously displayed some biological properties, similar to those of glycosaminoglycans (GAG), explored in cancer and tissue engineering. These GAG-mimetic derivatives are obtained through a free radical depolymerization process, which could, however, affect their structural integrity. In a previous study, we have shown that A. infernus produces depolymerizing enzymes active on its own... |
| Tipo: Text |
Palavras-chave: Deep-sea bacterium; Alteromonas infernus; Wild-type strain; Exopolysaccharides; Glycosaminoglycan-mimetic; Enzymatic depolymerization; Structural analysis; Mass spectrometry. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00515/62644/67021.pdf |
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| Delbarre-ladrat, Christine; Sinquin, Corinne; Lebellenger, Lou; Zykwinska, Agata; Colliec-jouault, Sylvia. |
| Although polysaccharides are ubiquitous and the most abundant renewable bio-components, their studies, covered by the glycochemistry and glycobiology fields, remain a challenge due to their high molecular diversity and complexity. Polysaccharides are industrially used in food products; human therapeutics fall into a more recent research field and pharmaceutical industry is looking for more and more molecules with enhanced activities. Glycosaminoglycans (GAGs) found in animal tissues play a critical role in cellular physiological and pathological processes as they bind many cellular components. Therefore, they present a great potential for the design and preparation of therapeutic drugs. On the other hand, microorganisms producing exopolysaccharides (EPS)... |
| Tipo: Text |
Palavras-chave: Marine-derivedpolysaccharide; Biodiversity; Biologicalactivity; Structure; Modification; GAG-mimetic; Biosynthesis. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00241/35250/33764.pdf |
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| Senni, Karim; Pereira, Jessica; Gueniche, Farida; Delbarre-ladrat, Christine; Sinquin, Corinne; Ratiskol, Jacqueline; Godeau, Gaston; Fischer, Anne-marie; Helley, Dominique; Colliec-jouault, Sylvia. |
| The therapeutic potential of natural bioactive compounds such as polysaccharides, especially glycosaminoglycans, is now well documented, and this activity combined with natural biodiversity will allow the development of a new generation of therapeutics. Advances in our understanding of the biosynthesis, structure and function of complex glycans from mammalian origin have shown the crucial role of this class of molecules to modulate disease processes and the importance of a deeper knowledge of structure-activity relationships. Marine environment offers a tremendous biodiversity and original polysaccharides have been discovered presenting a great chemical diversity that is largely species specific. The study of the biological properties of the... |
| Tipo: Text |
Palavras-chave: Marine bacteria; Marine algae; Exopolysaccharides; Sulfated polysaccharides; Structure; Chemical modification; Biological activity; Blue biotechnology; Cell therapy; Tissue engineering. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00048/15894/13316.pdf |
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| Mazza, Mattia; Alliot, Cyrille; Sinquin, Corinne; Colliec-jouault, Sylvia; Reiller, Pascal E.; Huclier-markai, Sandrine. |
| Background: Exopolysaccharide (EPS) derivatives, produced by Alteromonas infernus bacterium, showed anti-metastatic properties. They may represent a new class of ligands to be combined with theranostic radionuclides, such as 47Sc/44Sc. The goal of this work was to investigate the feasibility of such coupling. (2) Methods: EPSs, as well as heparin used as a drug reference, were characterized in terms of molar mass and dispersity using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering (AF4-MALS). The intrinsic viscosity of EPSs at different ionic strengths were measured in order to establish the conformation. To determine the stability constants of Sc with EPS and heparin, a Free-ion selective radiotracer extraction (FISRE)... |
| Tipo: Text |
Palavras-chave: Exopolysaccharides; Scandium; Theranostic; Characterization; Complexation. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00681/79332/81844.pdf |
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| Camacho-chab, Juan Carlos; Guezennec, Jean; Chan-bacab, Manuel Jesus; Rios-leal, Elvira; Sinquin, Corinne; Muniz-salazar, Raquel; De La Rosa-garcia, Susana Del C.; Reyes-estebanez, Manuela; Ortega-morales, Benjamin Otto. |
| A previously reported bacterial bioemulsifier, here termed microbactan, was further analyzed to characterize its lipid component, molecular weight, ionic character and toxicity, along with its bioemulsifying potential for hydrophobic substrates at a range of temperatures, salinities and pH values. Analyses showed that microbactan is a high molecular weight (700 kDa), non-ionic molecule. Gas chromatography of the lipid fraction revealed the presence of palmitic, stearic, and oleic acids; thus microbactan may be considered a glycolipoprotein. Microbactan emulsified aromatic hydrocarbons and oils to various extents; the highest emulsification index was recorded against motor oil (96%). The stability of the microbactan-motor oil emulsion model reached its... |
| Tipo: Text |
Palavras-chave: Bioemulsifier; Emulsifying stability; Non-toxic; Microbacterium sp; Biotechnology. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00372/48356/48545.pdf |
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| Colliec-jouault, Sylvia; Zanchetta, P; Helley, D; Ratiskol, Jacqueline; Sinquin, Corinne; Fischer, A. M.; Guezennec, Jean. |
| Bacterial polysaccharides offer fascinating potential applications for the pharmaceutical industry. Although many known marine bacteria produce exopolysaccharides (EPS), continuation in looking for new polysaccharide-producing micro-organisms is promising. Marine bacteria, isolated from deep-sea hydrothermal vents, have demonstrated their ability to produce in aerobic conditions, unusual EPS. With the aim of discovering biological activities, EPS presenting different structural features were studied. An EPS secreted by Vibrio, diabolicus was evaluated on the restoration of bone integrity in experimental model and was demonstrated to be a strong bone-healing material. Another EPS produced by Alteromonas infernus was modified in order to obtain new... |
| Tipo: Text |
Palavras-chave: Hydrothermal vents; Exopolysaccharides; Bone healing; Biomimetic; Anticoagulant activity; Sources hydrothermales; Exopolysaccharide; Cicatrisation osseuse; Biomimétique; Activité anticoagulante. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-2117.pdf |
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| Heymann, Dominique; Ruiz-velasco, Carmen; Chesneau, Julie; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia. |
| Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no... |
| Tipo: Text |
Palavras-chave: Exopolysaccharides; Glycosaminoglycan; Heparin-like; Derivatives; Sulfation; Bone metabolism; Bone remodeling; Lung mestatases; Osteosarcoma. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00333/44442/44112.pdf |
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| Matou, Sabine; Colliec-jouault, Sylvia; Galy Fauroux, Isabelle; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, Anne-marie; Helley, Dominique. |
| The aim of this study was to determine the angiogenic properties of an oversulfated exopolysaccharide (OS-EPS) derived from a polysaccharide secreted by the mesophilic bacterium Alteromonas infernus. We compared the effect of this OS-EPS with that of a non-oversulfated exopolysaccharide (EPS) on human umbilical vein endothelial cell (HUVEC) proliferation, migration and differentiation induced by basic fibroblast growth factor (FGF-2) or vascular endothelial growth factor (VEGF). OS-EPS enhanced HUVEC proliferation by 58% when used alone, and by respectively 30% and 70% in the presence of FGF-2 and VEGF. OS-EPS also increased the density of tubular structures on Matrigel in the presence of FGF-2 or VEGF. Vascular tube fort-nation was related to alpha(6)... |
| Tipo: Text |
Palavras-chave: Matrigel; Collagen gel; Alpha 6 integrin subunit; Angiogenic growth factor; Polysaccharide; Angiogenesis. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-2118.pdf |
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| Colliec-jouault, Sylvia; Chevolot, Lionel; Helley, Dominique; Ratiskol, Jacqueline; Bros, Andrée; Sinquin, Corinne; Roger, Olivier; Fischer, Anne-marie. |
| A new low-molecular-weight 'heparin-like' component was obtained from an exopolysaccharide produced by a mesophilic strain found in deep-sea hydrothermal vents. Data concerning the structure of the native high-molecular-weight exopolysaccharide (10(6) g/mol, 10% sulfate content) are reported for the first time. Two depolymerization processes were used to obtain low-molecular-weight (24-35 x 10(3) g/mol) oversulfated fractions (sulfate content 20 or 40%), Nuclear magnetic resonance studies indicated that after sulfation (40%), the low-molecular-weight fraction obtained by free radical depolymerization was less sulfated in the 6-O-position than the fraction depolymerized by acid hydrolysis. The free radical depolymerized product also had sulfated residues in... |
| Tipo: Text |
Palavras-chave: Sulfation; Sulfated polysaccharide; Serpin; Heparin like; Depolymerization; Anticoagulant activity; Affinity co electrophoresis. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-2111.pdf |
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| Roger, Olivier; Colliec-jouault, Sylvia; Ratiskol, Jacqueline; Sinquin, Corinne; Guezennec, Jean; Fischer, A; Chevolot, Lionel. |
| Three sulphated polysaccharides labelled at the reducing end with three different chemical treatments (2-aminopyridine (2-AP), 8-amino-1,3,6-trisulphonic acid (ANTS) and 6-(biotinyl)-aminocaproyl-hydrazide (BACH)) were compared. Molecular weight, chemical composition, nuclear magnetic resonance features and anticoagulant activity expressed as activated partial thromboplastin time were studied to determine whether structural and biological properties were retained or not after labelling. Partial depolymerisation and reduced biological activity were observed when reductive amination was performed with 2-AP. The initial properties were better retained with ANTS, and BACH did not modify the anticoagulant and structural properties. (C) 2002 Elsevier Science... |
| Tipo: Text |
Palavras-chave: Anticoagulant activity; Reductive amination; Fluorescent tagging; Fucan; GAG; Sulphated polysaccharide. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2113.pdf |
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| Han Ching, Lucay; Hadjadj, Annie; Sinquin, Corinne. |
| Les mollusques congelés représentent une part de plus en plus importante de nos importations de produits de la mer. Destinées à la consommation directe ou à la transformation, les noix de coquilles Saint Jacques congelées sont importées du Japon, du Royaume Uni ou d'Australie, en général glazurées individuellement, et souvent après trempage préalable destiné à les faire absorber une certaine quantité d'eau. C'est ainsi que l'eau totale ajoutée à la noix (glaçage + trempage) peut représenter 50 % du poids du produit avant traitement. A la demande conjointe de l'administration et de la profession, une méthode de détermination de l'eau de glaçage ajoutée a été mise au point au sein d'un groupe de travail animé par la FICUR. Dans ce même contexte, le besoin... |
| Tipo: Text |
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| Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00099/20988/18614.pdf |
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| Grivaud-le Du, Alicia; Zykwinska, Agata; Sinquin, Corinne; Ratiskol, Jacqueline; Weiss, Pierre; Vinatier, Claire; Guicheux, Jerome; Delbarre-ladrat, Christine; Colliec-jouault, Sylvia. |
| Alteromonas infernus bacterium isolated from deep-sea hydrothermal vents can produce by fermentation a high molecular weight exopolysaccharide (EPS) called GY785. This EPS described as a new source of glycosaminoglycan-like molecule presents a great potential for pharmaceutical and biotechnological applications. However, this unusual EPS is secreted by a Gram-negative bacterium and can be therefore contaminated by endotoxins, in particular the lipopolysaccharides (LPS). Biochemical and chemical analyses of the LPS extracted from A. infernus membranes have shown the lack of the typical LPS architecture since 3-deoxy-d-manno-oct-2-ulopyranosonic acid (Kdo), glucosamine (GlcN), and phosphorylated monosaccharides were not present. Unlike for other... |
| Tipo: Text |
Palavras-chave: Marine Alteromonas infernus; Bacterial exopolysaccharide; Endotoxin; Characterization; Purification; Fermentation; Process. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00397/50818/51601.pdf |
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| Senni, Karim; Gueniche, Farida; Changotade, Sylvie; Septier, Dominique; Sinquin, Corinne; Ratiskol, Jacqueline; Lutomski, Didier; Godeau, Gaston; Guezennec, Jean; Colliec-jouault, Sylvia. |
| Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800... |
| Tipo: Text |
Palavras-chave: Marine hydrothermal bacteria; Glycosaminoglycan-mimetic; Collagen; Matrix metalloproteinases; Dermal fibroblast; Polysaccharides; Tissue engineering. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00138/24973/23072.pdf |
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| Le Costaouec, Tinaig; Cerantola, Stephane; Ropartz, D.; Ratiskol, Jacqueline; Sinquin, Corinne; Colliec-jouault, Sylvia; Boisset, Claire. |
| Some marine bacteria collected around deep-sea hydrothermal vents are able to produce, in laboratory conditions, complex and innovative exopolysaccharides. In a previous study, the mesophilic strain Alteromonas macleodii subsp. fijiensis biovar deepsane was collected on the East Pacific Rise at 2600m depth. It was isolated from a polychaete annelid Alvinella pompejana and is able to synthesise and excrete the exopolysaccharide deepsane. Biological activities have been screened and some protective properties have been established. Deepsane is commercially available in cosmetics under the name of Abyssine (R) for soothing and reducing irritation of sensitive skin against chemical, mechanical and UVB aggression. This study presents structural data for this... |
| Tipo: Text |
Palavras-chave: Bacterial exopolysaccharide; Alteromonas macleodii; Structure determination; NMR spectroscopy; Mass spectrometry. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00095/20620/18536.pdf |
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| Registros recuperados: 35 | |
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