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| Registros recuperados: 30 | |
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| Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick. |
| This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf |
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| Pepin, Jean-francois; Soletchnik, Patrick; Le Moine, Olivier; Polsenaere, Pierre; Genauzeau, Sylvie; Robert, Stephane; Grizon, James; Seugnet, Jean-luc; Schmitt, Anne; Chabirand, Jean-michel; Tourbiez, Delphine; Travers, Marie-agnes; Guesdon, Stephane. |
| We report in this work data of two independent studies carried out in the Pertuis Charentais for which a link might exist? A 30-years local time series study of hydro-climatic variations in water temperature and salinity, which show a significant warming of the water masses in the Marennes Oleron bay (+ 1.5 °C) associated with a significant drop ( > -15%) of freshwater intakes by nearby rivers. In this changing local climate context several major epizootics have appeared, severely affecting farmers and the production of Pacific oysters and mussels, from the first French shellfish-producing department (catching and growing, Agreste, 2012). Following massive mortality event of blue mussels in 2014, a specific study (MORBLEU) is conducted, trying to... |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00415/52697/53567.pdf |
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| Schikorski, David; Renault, Tristan; Paillard, Christine; Bidault-toffin, A.; Tourbiez, Delphine; Saulnier, Denis. |
| The Gram-negative bacterium Vibrio harveyi is known to be highly pathogenic for the European abalone Haliotis tuberculata, which is a gastronomically important marine gastropod with a high commercial value. Since 1998, some particular bacterial strains are described as implicated in recurrent mortality outbreaks in French farm and field stocks of abalone. Recently, a 9.6kb plasmid named pVCR1, was shown to be harbored by one highly V. harveyi virulent ORM4 strain suggesting its involvement in virulence phenotype. Thus, we have developed in the present study two TaqMan real-time PCR assays allowing to (i) rapidly and specifically detect, by a duplex procedure and in less than 2 h, both V. harveyi and the presence of plasmid pVCR1 from unidentified bacterial... |
| Tipo: Text |
Palavras-chave: Vibrio harveyi; Haliotis tuberculata; Real-time PCR; Plasmid; Marine pathogen; Molecular diagnostic. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00124/23540/21383.pdf |
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| Francois, Cyrille; Garcia, Celine; Arzul, Isabelle; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Tourbiez, Delphine; Omnes, Emmanuelle; Cobret, Laetitia; Faury, Nicole; Haffner, Philippe; Saulnier, Denis; Pepin, Jean-francois; Renault, Tristan; Antajan, Elvire; Rauflet, Fabienne; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Chabirand, Jean-michel; Fillon, Alain; Robert, Stephane; Courtois, Olivier. |
| Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s'inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l'introduction et la propagation d'organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l'évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l'IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l'huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/00086/19709/17351.pdf |
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| Guichard, Benjamin; Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Saulnier, Denis; Pepin, Jean-francois; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Chollet, Bruno; Robert, Maeva; Renault, Tristan; Rauflet, Fabienne; Le Gagneur, Eric; Ropert, Michel; Mouillard, Gilbert; Gerla, Daniel; Annezo, Jean-pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Brerette, Stéphane; Chabirand, Jean-michel; Grizon, James; Robert, Stephane; Courtois, Olivier; Rumebe, Myriam; Cantin, Christian. |
| Créé en 1992, le réseau REPAMO (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à déclaration obligatoire et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font parties des missions institutionnelles de l’IFREMER. La surveillance assurée par le réseau vise en premier lieu les infections à déclaration obligatoire présentes en France, la bonamiose à Bonamia ostreae chez l’huître plate Ostrea edulis et la marteiliose à Marteilia refringens chez... |
| Tipo: Text |
Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Santé; Surveillance. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00059/17050/14599.pdf |
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| Lupo, Coralie; Travers, Marie-agnes; Tourbiez, Delphine; Barthélémy, Clement; Beaunée, Gaël; Ezanno, Pauline. |
| Vibrio aestuarianus is a bacterium related to mortality outbreaks in Pacific oysters, Crassostrea gigas, in France, Ireland, and Scotland since 2011. Knowledge about its transmission dynamics is still lacking, impairing guidance to prevent and control the related disease spread. Mathematical modeling is a relevant approach to better understand the determinants of a disease and predict its dynamics in imperfectly observed pathosystems. We developed here the first marine epidemiological model to estimate the key parameters of V. aestuarianus infection at a local scale in a small and closed oyster population under controlled laboratory conditions. Using a compartmental model accounting for free-living bacteria in seawater, we predicted the infection dynamics... |
| Tipo: Text |
Palavras-chave: Marine epidemiology; Parameter estimation; Compartmental model; ABC method; Global sensitivity analysis; Basic reproduction number R-0; Crassostrea gigas; Oyster mortality. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00495/60652/64148.pdf |
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| Lupo, Coralie; Dutta, Bhagat Lal; Petton, Sebastien; Ezanno, P; Tourbiez, Delphine; Travers, Marie-agnes; Pernet, Fabrice; Bacher, Cedric. |
| Vibrio aestuarianus infection in oyster populations causes massive mortality, resulting in losses for oyster farmers. Such dynamics result from host-pathogen interactions and contagion through water-borne transmission. To assess the spatiotemporal spread of V. aestuarianus infection and associated oyster mortality at a bay scale, we built a mathematical model informed by experimental infection data at 2 temperatures and spatially dependent marine connectivity of oyster farms. We applied the model to a real system and tested the importance of each factor using a number of modelling scenarios. Results suggest that introducing V. aestuarianus in a fully susceptible adult oyster population in the bay would lead to the mortality of all farmed oysters over 6 to... |
| Tipo: Text |
Palavras-chave: Basic reproduction number; Crassostrea gigas; Hydrodynamics; Oyster disease; Sensitivity analysis; Vibrio aestuarianus. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00662/77459/79114.pdf |
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| Travers, Marie-agnes; Mersni Achour, Rachida; Haffner, Philippe; Tourbiez, Delphine; Cassone, Anne-laure; Morga, Benjamin; Doghri, Ibtissem; Garcia, Celine; Renault, Tristan; Fruitier-arnaudin, Ingrid; Saulnier, Denis. |
| Nine dominant bacterial isolates were obtained from different batches of Crassostrea gigas spat experiencing high mortality rates in a French experimental hatchery/nursery in 2007. Using phenotypic analysis combined with multilocus sequence analysis, the isolates were shown to be genetically close to the Vibrio tubiashii type strain. Based on (1) analyses of the recA gene sequences; (2) the results of DNA–DNA hybridization assays between 07/118 T2 (LMG 27884 = CECT 8426), which is a representative strain, and the V. tubiashii type strain (69%); and (3) phenotypic traits, the bacteria were classified in a group close to American V. tubiashii strain. Its virulence (70% of mortalities) and the toxicity of the extracellular products of 07/118 T2 was... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas Pathogenicity Extracellular products Polyphasic approach; Real time PCR. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00189/30028/28743.pdf |
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| Benabdelmouna, Abdellah; Tourbiez, Delphine; D'Amico, Florence; Cantin, Christian; Grizon, James; Seugnet, Jean-luc. |
| En 2009, La caractérisation du niveau de ploïdie des naissains captés en 2008 dans les deux principaux bassins de captage que sont Marennes Oléron et Arcachon a été réalisée dans le cadre du réseau biovigilance. Les échantillons des naissains sauvages issus de ce captage naturel ont été prélevés sur 3 sites dans chaque bassin et pour un total analysé de 1121 animaux. En tenant compte de notre échantillonnage, nos résultats de suivi de ploïdie concluent à l’absence d’animaux polyploïdes, triploïdes ou tétraploïdes, captés dans les deux bassins prospectés. Cependant, les deux bassins de captage rospectés sont caractérisés par la présence accrue de naissains affectés de diverses anomalies génomiques (cassures d’ADN et diminution de la taille du génome,... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Génétique; Ploïdie; Naissain; Bassin Marennes Oléron; Charente-Maritime; Bassin d'Arcachon; Gironde. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00032/14282/11559.pdf |
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| Pepin, Jean-francois; Bouget, Jean-francois; Chabirand, Jean-michel; Chasselin, Leo; Costes, Louis; Grizon, James; Lejolivet, Aurore; Le Noc, Sandrine; Palvadeau, Hubert; Schmitt, Anne; Seugnet, Jean-luc; Soletchnik, Patrick; Travers, Marie-agnes; Tourbiez, Delphine; Guesdon, Stephane. |
| Since 2014 in France, in Charente maritime and Vendée, farmed and stocks of blue mussels experienced strong mortality events. In this context, the « MORBLEU » research project (DPMA-Ifremer agreement) was initiated to explore potentially aggravating factors, associated or correlated with mortality of mussels. Here, the work is to study biotic factors in mussels environment, looking for describe diversity and evolution of microbial communities (water column) before, during and after mortality events using NGS technology (metabarcoding) for environmental DNA analysis. We present, sampling strategy used from 2015 to 2018, Material & Methods and some preliminary results. |
| Tipo: Text |
Palavras-chave: MORBLEU; Moules; Mortalité; ADN environnemental; EDNA; Metabarcoding; Communautés microbiennes. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00505/61664/65579.pdf |
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| Registros recuperados: 30 | |
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