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| Registros recuperados: 54 | |
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| Degremont, Lionel; Lamy, Jean-baptiste; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan. |
| The Pacific oyster, Crassostrea gigas, is the most important commercial oyster species cultivated in the world. Meanwhile, the ostreid herpesvirus 1 (OsHV-1) is one of the major pathogens affecting the Pacific oyster, and numerous mortality outbreaks related to this pathogen are now reported worldwide. To assess the genetic basis of resistance to OsHV-1 infection in spat C. gigas and to facilitate breeding programs for such a trait, if any exist, we compared the mortality of half- and full-sib families using three field methods and a controlled challenge by OsHV-1 in the laboratory. In the field, three methods were tested: (A) one family per bag; (B) one family per small soft mesh bag and all families inside one bag; (C) same as the previous methods but... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00269/38066/36178.pdf |
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| Lopez-joven, Maria-carmen; Rolland, Jean-luc; Haffner, Philippe; Caro, Audrey; Roques, Cecile; Carre, Claire; Travers, Marie-agnes; Abadie, Eric; Laabir, Mohamed; Bonnet, Delphine; Destoumieux-garzon, Delphine. |
| Vibrio species have been associated with recurrent mass mortalities of juvenile oysters Crassostrea gigas threatening oyster farming worldwide. However, knowledge of the ecology of pathogens in affected oyster farming areas remains scarce. Specifically, there are no data regarding (i) the environmental reservoirs of Vibrio populations pathogenic to oysters, (ii) the environmental factors favoring their transmission, and (iii) the influence of oyster farming on the persistence of those pathogens. This knowledge gap limits our capacity to predict and mitigate disease occurrence. To address these issues, we monitored Vibrio species potentially pathogenic to C. gigas in 2013 and 2014 in the Thau Lagoon, a major oyster farming region in the coastal French... |
| Tipo: Text |
Palavras-chave: Vibrio; Shellfish farming; Bivalve mollusks; Mortality outbreak; Phytoplankton; Zooplankton. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00463/57479/59598.pdf |
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| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garrigues, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre; Renault, Tristan. |
| Mass mortality outbreaks (90-100%) in both juvenile and adult blue mussels were reported in 2014 along the Pertuis Charentais area of the French Atlantic coast. A series of studies were carried out to learn more about the phenomenon through detecting pathogens, describing environmental conditions and establishing the physiological status of the animals. Bacteria identif ied as belonging to the V. splendidus species were detected in dying mussels and seem able to cause mortality in blue mussels in the laboratory. Unusual environmental conditions (large amounts of fresh water, re-suspension of sediments during a series of storms, and a high renewal rate of specific water masses in Brittany’s Pertuis area) may be partially responsible for sparking the phenomen |
| Tipo: Text |
Palavras-chave: Mortalités; Moules; Pertuis Charentais; Vibrio splendidus; Environnement; Mortality; Mussels; Pertuis Charentais; Vibrio splendidus; Environment. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00312/42343/41682.pdf |
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| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre. |
| Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf |
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| Haenen, O. L. M.; Fouz, B.; Amaro, C.; Isern, M. M.; Mikkelsen, H.; Zrncic, S.; Travers, Marie-agnes; Renault, Tristan; Wardle, R.; Hellstrom, A.; Dalsgaard, I.. |
| Aquaculture in brackish and marine water is growing worldwide (FAO, 2014), including new cultured species. Various Vibrio infections occur, and cause significant problems, in fish, crustacean and shellfish (Austin & Austin, 2007. Vibrio anguillarum, V. salmonicida, V. ordalii and V. vulnificus are among the pathogens that lead to the biggest losses in aquaculture all over the world (Toranzo et al. 2005, Sandlund et al., 2010; Sitjà-Bobadilla et al. 2014). |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00211/32186/30615.pdf |
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| Azema, Patrick; Travers, Marie-agnes; De Lorgeril, Julien; Tourbiez, Delphine; Degremont, Lionel. |
| Since 2008, the emergent virus OsHV 1μvar has provoked massive mortality events in Crassostrea gigas spat and juveniles in France. Since 2012, mortality driven by the pathogenic bacteria Vibrio aestuarianus has stricken market sized adults. A hypothesis to explain the sudden increase in mortality observed in France since 2012 is that selective pressure due to recurrent viral infections could have led to a higher susceptibility of adults to Vibrio infection. In our study, two OsHV-1-resistant lines (AS and BS) and their respective controls (AC and BC) were experimentally challenged in the laboratory to determine their level of susceptibility to V. aestuarianus infection. At the juvenile stage, the selected lines exhibited lower mortality (14 and 33%) than... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00299/41054/40215.pdf |
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| Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Tourbiez, Delphine; Chollet, Bruno; Faury, Nicole; Haffner, Philippe; Dubreuil, Christine; Serpin, Delphine; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Cheve, Julien; Penot, Julia; Le Gal, Dominique; Lebrun, Luc; Le Gac-abernot, Chantal; Langlade, Aime; Bedier, Edouard; Palvadeau, Hubert; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrick; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude. |
| Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Repamo a pour objectif de détecter précocement les infections dues à des organismes pathogènes exotiques et émergents affectant les mollusques marins sauvages et d’élevage. L’étude des hausses de mortalités a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la collecte d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités d’huîtres creuses (Crassostrea gigas) (44 interventions) ont été recensées dans la majorité des bassins de production de mai à... |
| Tipo: Text |
Palavras-chave: Réseau Surveillance; Pathologie; Mollusques; Coquillages; Santé. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00197/30798/29167.pdf |
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| Segarra, Amelie; Faury, Nicole; Haffner, Philippe; Pepin, Jean-francois; Tourbiez, Delphine; Trancart, Suzanne; Travers, Marie-agnes; Renault, Tristan. |
| Le premier cas d'infection à virus de type herpès a été rapporté en 1972, aux Etats Unis, chez l'huître américaine, Crassostrea virginica (Farley et al, 1972). A partir de 1991, des mortalités massives associées à la détection de virus de type herpes ont été observées chez plusieurs espèces de bivalves marins, à différents stades de développement (essentiellement chez les larves et le naissain) et dans différentes régions du monde. Sur la base d’observation en microscopie électronique à transmission, ces virus présentaient des caractéristiques morphologiques identiques à celles des Herpesvirus (Roizman, 1982). Les connaissances acquises concernant la taille, la structure et le séquençage du génome d’un virus infectant les larves d’huîtres creuses en... |
| Tipo: Text |
Palavras-chave: Herpès virus OsHV-1; Expression gènes; Huître creuse; Crassostrea gigas. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00195/30627/29076.pdf |
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| Pepin, Jean-francois; Benabdelmouna, Abdellah; Bierne, Nicolas; Costes, Louis; Degremont, Lionel; Garcia, Celine; Guesdon, Stephane; Lamy, Jean-baptiste; Le Moine, Olivier; Morga, Benjamin; Normand, Julien; Robert, Stephane; Saunier, Alice; Soletchnik, Patrick; Travers, Marie-agnes. |
| Depuis 2014, des épisodes de mortalité anormale affectent les cheptels mytilicoles tant adultes que juvéniles. Dès l’année 2015, une étude spécifique cofinancée par la DPMA et réalisée par l’Ifremer a été initiée, l’action de recherche MORBLEU (Mortalité des moules Bleues). Les années 2015 et 2016 ont permis de dégager des éléments de connaissance et des pistes relatifs aux facteurs biotiques et abiotiques qui semblent intervenir dans le phénomène de hausse de mortalité des moules depuis 2014. Pour l’année 2017, les trois objectifs principaux étaient de : i) poursuivre la description du phénomène de mortalité ainsi que le contexte environnemental associé en effectuant une nouvelle campagne d’études et d’observations in situ, ii) tester des hypothèses... |
| Tipo: Text |
Palavras-chave: Moules; Mytilus edulis; Mortalité; Statut cytogénétique; Anomalies génomiques; Populations; Facteurs environnementaux; Ecologie microbienne. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00503/61503/65337.pdf |
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| Francois, Cyrille; Joly, Jean-pierre; Garcia, Celine; Lupo, Coralie; Travers, Marie-agnes; Pepin, Jean-francois; Hatt, Philippe-jacques; Arzul, Isabelle; Omnes, Emmanuelle; Tourbiez, Delphine; Faury, Nicole; Haffner, Philippe; Huchet, Eve; Dubreuil, Christine; Chollet, Bruno; Renault, Tristan; Cordier, Remy; Hebert, Pascale; Le Gagneur, Eric; Parrad, Sophie; Gerla, Daniel; Annezo, Jean-pierre; Terre-terrillon, Aouregan; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Hittier, Benoist; Grizon, James; Chabirand, Jean-michel; Robert, Stephane; Seugnet, Jean-luc; Rumebe, Myriam; Le Gall, Patrik; Bouchoucha, Marc; Baldi, Yoann; Masson, Jean-claude. |
| Créé en 1992, le réseau Repamo (REseau de PAthologie des MOllusques) est un réseau de surveillance de la santé des mollusques marins du littoral français. Son activité s’inscrit dans le cadre de la Directive Européenne 2006/88/CE. Les objectifs du réseau sont de prévenir l’introduction et la propagation d’organismes pathogènes, en particulier ceux à réglementés et de surveiller l’évolution de ceux déjà présents sur le territoire national. Ces activités font partie des missions institutionnelles de l’IFREMER. L’étude des hausses de mortalités (protocole II) a été poursuivie avec 52 interventions complètes ayant conduit au recueil de commémoratifs et à la réalisation d’échantillons de mollusques marins pour analyses en pathologie. Des hausses de mortalités... |
| Tipo: Text |
Palavras-chave: Réseau; Pathologie; Mollusques; Coquillages; Santé; Surveillance. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00143/25470/23625.pdf |
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| Nasfi, H.; Travers, Marie-agnes; De Lorgeril, Julien; Habib, C.; Sannie, T.; Sorieul, L.; Gerard, J.; Avarre, J. C.; Haffner, Philippe; Tourbiez, Delphine; Renault, Tristan; Furones, D.; Roque, A.; Pruzzo, C.; Cheslett, D.; Gdoura, R.; Vallaeys, T.. |
| The Vibrio splendidus clade has previously been associated with epidemic outbreaks of various aquatic animals, as in the case of the cupped oyster, Crassostrea gigas. To investigate whether involved strains could present a clonal origin and to identify possible alternative background carriage animals or zooplankton, a large epidemiological survey was conducted on isolates of the splendidus clade. For this purpose, Vibrio strains were isolated from various samples including oysters, mussels, sediments, zooplankton, and sea water on the basis of a North/South gradient of the European sea water zone (Ireland, The Netherlands, France, Italy, and Spain). A total of 435 isolates were successfully associated to the V. splendidus clade using real time polymerase... |
| Tipo: Text |
Palavras-chave: VNTR; Vibrio splendidus; Epidemiology; Vsm; OmpU; Crassotrea giga; Mortality. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00255/36666/35350.pdf |
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| Garcia, Celine; Mesnil, Aurelie; Tourbiez, Delphine; Moussa Pouly, Mirna; Dubreuil, Christine; Gonçalves De Sa, Amélie; Chollet, Bruno; Godfrin, Yoann; Dégremont, Lionel; Serpin, Delphine; Travers, Marie-agnes. |
| Cockle mortality events have been reported in northern France since 2012. In the present study, we describe and investigate the implication of a potential bacterial causative agent in cockle mortality. Bacteria isolated from five different cockle mortality events were characterized and studied. Using phenotypic analysis combined with DNA–DNA hybridization (DDH) and whole genome sequencing, the isolates were shown to belong to Vibrio aestuarianus , a species regularly detected in France during oyster mortality events. Comparison of the strains from cockles with strains from French oysters and the type strain showed that the strains from cockles were genetically different to those from oysters and also different to the V. aestuarianus type strain. Moreover,... |
| Tipo: Text |
Palavras-chave: Bacterial pathogen; Bivalvia; Cockle; Mortality; Shellfish; Vibrio aestuarianus. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00677/78893/81195.pdf |
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| Ben Cheikh, Yosra; Travers, Marie-agnes; Le Foll, Frank. |
| The pathogenic strain V. splendidus 10/068 1T1 has previously been reported for its virulence to the blue mussel and for its capacity to alter immune responses. In this study, we expanded the knowledge on hemocyte-pathogen interactions by using in vitro and in vivo assays. V. splendidus 10/068 1T1 severely inhibited cell adhesion and acidic vacuole formation unlike the innocuous phylogenetically related V. splendidus 12/056 M24T1 which had no effect on these cell functions. Furthermore, the virulent bacteria decreased hemocyte viability (59% of viability after 24 h). Infection dynamics were explored by using a model based on water tank cohabitation with septic mussels infected by GFP-tagged V. splendidus 10/068 1T1. Experimental infections were... |
| Tipo: Text |
Palavras-chave: Innate immunity; Bivalves; Vibrio; Infection; Experimental model; Hemocyte. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00400/51136/53772.pdf |
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| Degremont, Lionel; Maurouard, Elise; Godfrin, Yoann; Travers, Marie-agnes; Morga, Benjamin; Lamy, Jean-baptiste; Benabdelmouna, Abdellah. |
| Depuis 2012, le nombre de cas de mortalités d’huîtres creuses adultes (Crassostrea gigas) rapportés dans le cadre des réseaux de surveillance de l’Ifremer, et dans lesquels la bactérie Vibrio aestuarianus a été isolée, a fortement augmenté. Les objectifs de cette étude étaient (1) d’étudier la sensibilité des huîtres C. gigas à une infection à V. aestuarianus pour des lots diploïdes et triploïdes et (2) déterminer la réponse à la sélection pour une meilleure résistance à V. aestuarianus pour des huîtres C. gigas. Neuf stocks ont été utilisés, répartis en trois niveaux de sélection, chacun étant donc répliqué par trois pontes. Pour chaque stock, un lot diploïde (2n), un lot triploïde (3n_chim) produit par la rétention d’un globule polaire, et trois lots... |
| Tipo: Text |
Palavras-chave: Vibrio aestuarianus; OsHV-1; Sélection; Résistance; Triploïdes; Huître creuse; Crassostrea gigas; Vibrio aestuarianus; OsHV-1; Selective breeding; Disease resistance Pacific oyster; Crassostrea gigas; Triploids. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00404/51530/52140.pdf |
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| Travers, Marie-agnes; Tourbiez, Delphine; Parizadeh, Leila; Haffner, Philippe; Kozic-djellouli, Angelique; Aboubaker, Mohamed; Koken, Marcel; Degremont, Lionel; Lupo, Coralie. |
| This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by... |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00386/49768/50308.pdf |
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| Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick. |
| This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf |
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| Mersni-achour, Rachida; Ben Cheikh, Yosra; Pichereau, Vianney; Doghri, Ibtissem; Etien, Cedric; Degremont, Lionel; Saulnier, Denis; Fruitier-arnaudin, Ingrid; Travers, Marie-agnes. |
| Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs causing bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with a LD50 about 2.3 × 103 cfu ml-1 after 24 h. Moreover, complete or GP- HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECPs fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by... |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00252/36367/34907.pdf |
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| Registros recuperados: 54 | |
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