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Registros recuperados: 22
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Evaluation of the Mechanism Responsible for the Low-Level Resistance to Glyphosate in Goosegrass Planta Daninha
ULGUIM,A.R.; FRANCO,J.J.; SILVA,J.D.G.; AGOSTINETTO,D.; VARGAS,L..
ABSTRACT Goosegrass (Eleusine indica) infests various crops, being reported as low-level resistant (LLR) to glyphosate. The objectives of this study were to evaluate the mechanisms of resistance to glyphosate in goosegrass by the cyt-P450 complex to identify changes in leaf anatomy of these plants and quantify the accumulation of shikimate in biotypes susceptible and with LLR. The metabolic rate was assessed by applying the cyt-P450 malathion inhibitor 30 minutes before the herbicide application. The analysis of the leaf anatomy consisted of a characterization of anatomic cross-sections and evaluation of the effect of glyphosate on the leaf epidermis and the conducting vessels. Plant tissue was collected 24 hours after treatment (HAT) of the dose-response...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cyt-P450 monooxygenase; Shikimate; Absorption and translocation; EPSPs; Eleusine indica.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100206
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IDENTIFICATION AND VALIDATION OF REFERENCE GENES FOR THE NORMALIZATION IN REAL-TIME RT-QPCR ON RICE AND RED RICE IN COMPETITION, UNDER DIFFERENT NITROGEN DOSES Planta Daninha
BENEMANN,D.P.; NOHATO,A.M.; VARGAS,L.; AVILA,L.A; AGOSTINETTO,D..
ABSTRACT Real time reverse transcription polymerase chain reaction (RT-qPCR) is an important technique to analyze differences in gene expression due to its sensitivity, accuracy, and specificity. However, before analyzing the expression of the target gene, it is necessary to identify and evaluate the stability of candidate reference genes for the proper normalization. This study aimed at evaluating the stability of candidate reference genes in order to identify the most appropriate genes for the normalization of the transcription in rice and red rice in competition under different nitrogen levels, as well as to demonstrate the effectiveness of the reference gene selected for the expression of the cytosolic ascorbate peroxidase (OsAPX2). Eleven candidate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oryza sativa; Gene expression; Normalization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582017000100215
Registros recuperados: 22
Primeira ... 12 ... Última
 

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