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Registros recuperados: 29
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EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO BJM
Pires,Maria de Fátima Costa; Corrêa,Benedito; Gambale,Walderez; Paula,Claudete Rodrigues.
The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Adherence; Serotypes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300001
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Adhesive and invasive capacities of Edwarsiella tarda isolated from South American sea lion BJM
Fernández,Araceli; Paz Villanueva,María; González,Mario; Fernández,Fabiola; Latif,Fadua; Flores,Sandra Nonier; Fernández,Heriberto.
Edwarsiella tarda is a zoonotic bacterium that can be isolated from humans, animals and the environment. Although E. tarda is primarily considered a fish pathogen, it is the only species of its genus considered to be pathogenic for humans as well. A survey of zoonotic intestinal bacteria in fresh feces from South American sea lions (SASL) Otaria flavescens, reported E. tarda as the most frequently isolated species. In this study, we used HEp-2 cells to establish in vitro the adherence and invasive ability of 17 E. tarda strains isolated from SASL fecal material. All the strains were able to adhere and invade HEp-2 cells with adhesion and invasion percentages ranging from 56 to 100% and 21 to 74%, respectively. Despite the expression of these pathogenic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Edwarsiella tarda; Adherence; Invasion; Otaria flavescens.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300044
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Analysis of the in vitro adherence of Streptococcus mutans and Candida albicans BJM
Barbieri,Dicler de Sant'Anna Vitor; Vicente,Vânia Aparecida; Fraiz,Fabian Calixto; Lavoranti,Osmir José; Svidzinski,Terezinha Inês Estivalet; Pinheiro,Rosângela Lameira.
The objective of the present study was to investigate the in vitro adherence capacity of Streptococcus mutans and Candida albicans. Adherence assays were conducted on dental surfaces and analyzed by scanning electron microscopy (SEM). Extracted human teeth were inoculated with Streptococcus mutans or Candida albicans and with both species simultaneously, and incubated at 37ºC for 21 days. Bacterial inocula had been obtained from saliva samples of children that had been colonized by both organisms. ATCC reference strains were used as controls. SEM analyses showed that the biofilm that covered the entire analyzed dental surface was more homogeneous inoculated with the two microorganisms simultaneously than with each species separately. In a second...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adherence; Candida albicans; Streptococcus mutans; Biofilm.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400009
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Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile BJM
Matter,Letícia B.; Spricigo,Denis A.; Tasca,Caiane; de Vargas,Agueda C..
The invasin gimB (genetic island associated with human newborn meningitis) is usually found in ExPEC (Extraintestinal Pathogenic Escherichia coli) such as UPEC (uropathogenic E. coli), NMEC (neonatal meningitis E. coli) and APEC (avian pathogenic E. coli). In NMEC, gimB is associated with the invasion process of the host cells. Due to the importance of E. coli as a zoonotic agent and the scarce information about the frequency of gimB-carrying strains in different animal species, the aim of this study was to investigate the presence of gimB in isolates from bovine, swine, canine and feline clinical samples. PCR was conducted on 196 isolates and the identity of the amplicons was confirmed by sequencing. Of the samples tested, only E. coli SB278/94 from a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GimB; Adherence; Invasiveness; Zoonotic potential; Livestock; Companion animals.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300875
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Adhesion, biofilm and genotypic characteristics of antimicrobial resistant Escherichia coli isolates BJM
Cergole-Novella,Maria C.; Pignatari,Antonio C.C.; Guth,Beatriz E.C..
Aggregative adherence to human epithelial cells, most to renal proximal tubular (HK-2) cells, and biofilm formation was identified among antimicrobial resistant Escherichia coli strains mainly isolated from bacteremia. The importance of these virulence properties contributing to host colonization and infection associated with multiresistant E. coli should not be neglected.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adherence; Biofilm; Extraintestinal Escherichia coli; Antimicrobial resistance; Virulence genes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100167
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Influence of subinhibitory concentrations of antimicrobials on hydrophobicity, adherence and ultra-structure of Fusobacterium nucleatum BJM
Okamoto,Ana C.; Gaetti-Jardim Jr.,Elerson; Arana-Chavez,Victor E.; Avila-Campos,Mario J..
Fusobacterium nucleatum is considered a bridge organism between earlier and later colonizers in dental biofilms and a putative periodontopathogen. In Dentistry, antimicrobial agents are used for treatment and control of infectious diseases associated with dental plaque. Antiseptics have been used in association with antibiotics to reduce infections after oral surgeries. In this study, the influence of subinhibitory concentrations (SC) of chlorhexidine, triclosan, penicillin G and metronidazole, on hydrophobicity, adherence to oral epithelial cells, and ultra-structure of F. nucleatum was examined. All isolates were susceptible to chlorhexidine, triclosan, and metronidazole; however, most of the isolates were susceptible to penicillin G, and all of them...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusobacterium nucleatum; Hydrophobicity; Adherence; Antimicrobial; Ultra-structure.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200017
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Aggregatibacter actinomycetemcomitansarcB influences hydrophobic properties, biofilm formation and adhesion to hydroxyapatite BJM
Longo,PL; Ota-Tsuzuki,C; Nunes,ACR; Fernandes,BL; Mintz,K; Fives-Taylor,P; Mayer,MPA.
The regulation of gene expression in the oral pathogen Aggregatibacter actinomycetemcomitans is still not fully elucidated. ArcAB is a two-component system which allows facultative anaerobic bacteria to sense various respiratory growth conditions and adapt their gene expression accordingly. This study investigated in A. actinomycetemcomitans the role of arcB on the regulation of biofilm formation, adhesion to saliva coated hydroxyapatite (SHA) and the hydrophobic properties of the cell. These phenotypic traits were determined for an A. actinomycetemcomitansarcB deficient type and a wild type strain. Differences in hydrophobic properties were shown at early and late exponential growth phases under microaerobic incubation and at late exponential phase under...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Two component system; Gene regulation; Adherence; Colonization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300018
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Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus BJM
Abdallah,Fethi Ben; Chaieb,Kamel; Zmantar,Tarek; Kallel,Hela; Bakhrouf,Amina.
In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio; Slime production; Adherence; Abiotic surface; Vero cells.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200033
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Blood pressure levels and adherence to treatment of hypertensive patients, users of a school pharmacy BJPS
Santos,Brigitte Rieckmann Martins dos; Teixeira,Juliana; Gonçalves,Débora; Gatti,Reynaldo Mascagni; Yavo,Boni; Aragão,Cicera Cristina Vidal.
Adherence to pharmacological treatment for hypertension is considered a key factor in guaranteeing successful therapy outcomes. Knowledge of the disease, its complications, as well as the need for changes in lifestyle, call for patient motivation and continuous interactive education. The evidence regarding the beneficial effects of changes in life style by hypertensive individuals in reducing the complications of the disease, as well as in its prevention are indisputable. However, the challenges posed by patient adherence to treatment prescribed by doctors remain. The aim of this study was to assess blood pressure levels together with degree of adherence to pharmacological treatment with Enalapril Maleate by means of the Morisky-Green Test, in hypertensive...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adherence; Hypertension; Health Promotion.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300004
Registros recuperados: 29
Primeira ... 12 ... Última
 

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