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| Registros recuperados: 47 | |
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| Marques, Odair José; UEM; Vidigal Filho, Pedro Soares; UEM; Dalpasquale, Valdecir Antoninho; UEM; Scapim, Carlos Alberto; UEM; Pricinotto, Luiz Fernando; UEM; Machinski Júnior, Miguel; UEM. |
| O objetivo deste trabalho foi avaliar a incidência de fungos dos gêneros Aspergillus, Fusarium e Penicillium e as contaminações com micotoxinas em grãos de cinco híbridos comerciais de milho em função da umidade de colheita. O trabalho foi conduzido em Astorga, Estado do Paraná, durante o período da ‘safrinha’ de 2007 e da safra de ‘verão’ de 2007/2008. As amostras de grãos, colhidas em cinco épocas distintas e em cinco repetições, foram submetidas à determinação de umidade pelo método da estufa a 103 ± 1ºC por 72h. O teste de sanidade Blotter test e a quantificação micotoxinas (método da cromatografia em camada delgada) foram efetuados, utilizando-se um delineamento inteiramente casualizado. Os resultados obtidos foram submetidos à análise de... |
| Tipo: Pesquisa Empírica de Campo |
Palavras-chave: 5.01.03.04-0 Produção de Mudas Zea mays L.; Pós-colheita; Qualidade; Aflatoxinas; Zearalenona Fitotecnia Zea mays L.; Post-harvest; Quality; Aflatoxins; Zearalenone. |
| Ano: 2009 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5690 |
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| Ono,Elisabete Yurie Sataque; Silva,Marcelo da; Ribeiro,Ricardo Marcelo Reche; Ono,Mario Augusto; Hayashi,Luciana; Garcia,Glauco Tironi; Hirooka,Elisa Yoko. |
| In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thin-Layer Chromatography; Spectrofluorimetry; BGYF; Aflatoxins; Corn; Mycotoxins. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300025 |
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| MARTINS,Hermínia Marina; MARTINS,Maria Lígia; BERNARDO,Fernando Almeida. |
| Aflatoxins production is affected by abiotic, biotic and genetic parameters. Eugenesic and dysgenetic condition for aflatoxinogenesis are relatively well studied, such as: the influence of temperature, pH, Aw, Oxigen tension, osmotic pression, organic and inorganic nutrients, substance with fungistatic effects: however there are relatively few knowledge about endogenous regulator mechanisms, the kinetic of the anabolism and the interaction between the productive moulds and remaining microflora holding in eutrophying substrates. The main objective of this study is to evaluate biotic interaction between five indigenous strains of A. flavus, confirmed non-toxigenic, and a productive strain (A. parasiticus ATCC 15 517), cultured simultaneously on two... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant interaction; Aflatoxins; Vegetal production; Aspergillus flavus; Aspergillus parasiticus. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600003 |
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| Herzog-Soares,Joanna D'Arc A; Freire,Ronald Bastos. |
| Macrophages exposed to 10 mug/mL citrinin (CTR) or 0.01 mug CTR mixed with 0.04 mug aflatoxin B1 (AFB1) for a period of 2 h at 37ºC, were infected with 10(6) Toxoplasma gondii tachyzoites/muL. The parasites were treated with mycotoxins (2 h at 37ºC) before being added to the macrophage culture. The number of tachyzoites was quantified 2, 24, 48, 72 and 96 h after infection. During the first 2 hours, 59% infectivity was observed in the control. After exposure to CTR or the mixture of toxins (CTR-AFB1), macrophages were infected with 77.5% and 75% of the inoculated tachyzoites, respectively. Similarly, 72.3% of the cells were infected when cultured together with previously treated parasites. The treatment with CTR-AFB1 gave rise to 2.9 times more tachyzoites... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aflatoxins; Imunossuspression; Macrophages; Citrinin; Toxoplasma gondii. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000100007 |
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| Gonçalez,E.; Felicio,J.D.; Pinto,M.M.. |
| The biflavonoids 6,6"-bigenkwanin, amenthoflavone, 7,7"-dimethoxyagastisflavone and tetradimethoxybigenkwanin isolated from Ouratea species were tested for inhibitory activity on Aspergillus flavus cultures. Suspensions of Aspergillus flavus spores were inoculated into 50 ml of YES medium at different biflavonoid concentrations: 5 and 10 µg/ml for 6,6"-bigenkwanin, amenthoflavone and 7,7"-dimethoxyagastisflavone, and 5, 10, 15 and 20 µg/ml for tetradimethoxybigenkwanin. The four biflavonoids showed inhibitory activity on aflatoxin B1 and B2 production (P<0.001), but did not inhibit fungal growth at the concentration tested (P>0.05). These results show that biflavonoids can be used for the development of agents to control aflatoxin production. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Ouratea sp; Aspergillus flavus; Biflavonoids; Aflatoxins. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100013 |
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| Registros recuperados: 47 | |
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