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Registros recuperados: 24
Primeira ... 12 ... Última
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Influence of antibiotics on embryogenic tissue and Agrobacterium tumefaciens suppression in soybean genetic transformation Bragantia
Wiebke,Beatriz; Ferreira,Fabricio; Pasquali,Giancarlo; Bodanese-Zanettini,Maria Helena; Droste,Annette.
The influence of different antibiotic treatments in soybean genetic transformation was evaluated. First, an assay was performed to verify how different antibiotic treatments affect soybean embryogenic tissues. The effect of carbenicillin at 500 mg L-1 was genotype-dependent. This antibiotic did not affect embryo survival of cv. IAS5, but a three-fold increase of embryo proliferation was observed for cv. Bragg, when compared to the control. On the other hand, cefotaxime at 350 and 500 mg L-1 caused death of embryogenic tissues of both cultivars. Finally, the association of cefotaxime (250 mg L-1) + vancomycin (250 mg L-1) did not affect negatively the somatic embryos of tested cultivars until 63 days of treatment. Thereafter, a second experiment was carried...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agrobacterium tumefaciens; Carbenicillin; Cefotaxime; Genetic transformation; Glycine max; Vancomycin.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052006000400002
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Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis Cotiledonary culture BABT
Quisen,Regina; Oliveira,Yohana de; Pileggi,Marcos; Cuquel,Francine; Quoirin,Marguerite.
The objectives of the present work were to establish the minimal lethal dose of the selective agent to determine the type and concentration of appropriate antibiotics for the elimination of Agrobacterium tumefaciens inoculated explants, without interfering with the regenerative potential of the E. camaldulensis cotyledonary explants. Non-transformed explants were cultivated in medium supplemented with kanamycin. The results showed that the antibiotic was suitable for the selection of transformed cells in the concentration of 9 mg L-1 as it inhibited the growth of non-transformed cells. Cotyledons infected with A. tumefaciens were cultivated in MS N/2 medium supplemented with BAP, ANA, Km and cefotaxime or AugmentinÒ . The highest average of regenerated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphogenesis; Kanamycin; Cefotaxime; Augmentin; Agrobacterium tumefaciens; Eucalypt.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600020
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Plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene BABT
Duan,Hong Ying; Ding,Xiao Sheng; Song,Jian Ying; He,Yun Long; Zhou,Yan Qing.
The aim of this work was to study the plant regeneration and Agrobacterium-mediated transformation of Achyranthes bidentata using cotton EREBP gene. Results showed that the callus induction rate of stems from A. bidentata was the highest (100%) and bud was in approximately 70% of calli from stems. However bud differentiation rate of the callus from leaves and petioles was very low. Compared with ceftriaxone, 200mg/L cefotaxime could completely control Agrobacterium tumefaciens and had relatively less toxic action on the stems of A. bidentata. In addition, the induction rate of callus resistant to hygromycin was the highest when infected for 3 min and co-cultivated for 3 d. Six positive transgenic plants transformed with pCAMBIA1304-GhEREB2 expression...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Achyranthes bidentata; EREBP gene; Agrobacterium tumefaciens; Genetic transformation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300001
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Relative susceptibility of peach rootstocks to crown gall and Phytophthora root and crown rot in Chile Ciencia e Investigación Agraria
Guzman,Gonzalo; Latorre,Bernardo A; Torres,Rene; Wilcox,Wayne F.
Crown gall (Agrobacterium tumefaciens) and Phytophthora root and crown rot (PRCR), caused by P. cryptogea, are two major limiting factors affecting peach (Prunus pérsica) production in Chile. At present, Nemaguard is the most important rootstock used in peach orchards, and although several clonal rootstocks are under development worldwide, there is little information regarding their susceptibility to crown gall and PRCR. Therefore this investigation was undertaken to study the susceptibility to A. tumefaciens and P. cryptogea of the clonally propagated rootstocks Cadman®-Avimag (P. pérsica x P. davidiana), Mr.S 2/5 (P. cerasifera x P. spinosa), Viking ([P. pérsica x P. davidiana] x [P. dulcís 'Jordanolo'x P. blireiana]), and Nemaguard (P. pérsica x P....
Tipo: Journal article Palavras-chave: Agrobacterium tumefaciens; Disease control; Nectarines; Rootstock resistance.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202007000100004
Registros recuperados: 24
Primeira ... 12 ... Última
 

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