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	Provedor de dados BJMBR (1) Autor Cao,Bin (1) Ren,Keyu (1) Wang,Jin (1) Yong,Chunming (1) Yuan,Hao (1) Zhao,Kun (1) Mais... Palavra-chave Ghrelin (1) Interstitial cells of Cajal (1) NF-κB (1) SCF (1) Substance P (1) TNF-α (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2018 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 38 Primeira ... 1 2 ... Última Culture medium for amylase production by toxigenic fungi BABT Figueira,Edson Luiz Zangrando; Hirooka,Elisa Yoko. Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p<0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amylase; Fusarium moniliforme; Aspergillus flavus. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500003 Interaction of strains of non-toxigenic Aspergillus flavus with Aspergillus parasiticus on aflatoxin production Braz. J. Vet. Res. Anim. Sci. MARTINS,Hermínia Marina; MARTINS,Maria Lígia; BERNARDO,Fernando Almeida. Aflatoxins production is affected by abiotic, biotic and genetic parameters. Eugenesic and dysgenetic condition for aflatoxinogenesis are relatively well studied, such as: the influence of temperature, pH, Aw, Oxigen tension, osmotic pression, organic and inorganic nutrients, substance with fungistatic effects: however there are relatively few knowledge about endogenous regulator mechanisms, the kinetic of the anabolism and the interaction between the productive moulds and remaining microflora holding in eutrophying substrates. The main objective of this study is to evaluate biotic interaction between five indigenous strains of A. flavus, confirmed non-toxigenic, and a productive strain (A. parasiticus ATCC 15 517), cultured simultaneously on two... Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant interaction; Aflatoxins; Vegetal production; Aspergillus flavus; Aspergillus parasiticus. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000600003 In vitro antifungal susceptibility of clinical and environmental isolates of Aspergillus fumigatus and Aspergillus flavus in Brazil BJID Denardi,Laura Bedin; Hoch Dalla-Lana,Bianca; Pantella Kunz de Jesus,Francielli; Bittencourt Severo,Cecília; Santurio,Janio Morais; Zanette,Régis Adriel; Alves,Sydney Hartz. ABSTRACT The in vitro susceptibility of 105 clinical and environmental strains of Aspergillus fumigatus and Aspergillus flavus to antifungal drugs, such as amphotericin B, azoles, and echinocandins was evaluated by the broth microdilution method proposed by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Following the EUCAST-proposed breakpoints, 20% and 25% of the clinical and environmental isolates of A. fumigatus, respectively, were found to be resistant to itraconazole (Minimal Inhibitory Concentration, MIC > 2.0 mg/L). Voriconazole showed good activity against A. fumigatus and A. flavus strains, except for one clinical strain of A. fumigatus whose MIC was 4.0 mg/L. Posaconazole (≤0.25 mg/L) also showed appreciable activity... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Aspergillus fumigatus; Echinocandins; Azoles; Environmental; Susceptibility. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000100030 Biflavonoids inhibit the production of aflatoxin by Aspergillus flavus BJMBR Gonçalez,E.; Felicio,J.D.; Pinto,M.M.. The biflavonoids 6,6"-bigenkwanin, amenthoflavone, 7,7"-dimethoxyagastisflavone and tetradimethoxybigenkwanin isolated from Ouratea species were tested for inhibitory activity on Aspergillus flavus cultures. Suspensions of Aspergillus flavus spores were inoculated into 50 ml of YES medium at different biflavonoid concentrations: 5 and 10 µg/ml for 6,6"-bigenkwanin, amenthoflavone and 7,7"-dimethoxyagastisflavone, and 5, 10, 15 and 20 µg/ml for tetradimethoxybigenkwanin. The four biflavonoids showed inhibitory activity on aflatoxin B1 and B2 production (P<0.001), but did not inhibit fungal growth at the concentration tested (P>0.05). These results show that biflavonoids can be used for the development of agents to control aflatoxin production. Tipo: Info:eu-repo/semantics/other Palavras-chave: Ouratea sp; Aspergillus flavus; Biflavonoids; Aflatoxins. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001100013 Nutritional changes in powdered red pepper upon in vitro infection of Aspergillus flavus BJM Tripathi,Smita; Mishra,H.N.. Quantitative losses in various biochemical constituents like capsaicin, carotenes, ascorbic acid, polyphenols, mineral matter, sugars (soluble and insoluble), protein and fat were estimated after the successful growth of Aspergillus flavus for 30 days on powdered red pepper. The fungal biomass was measured by ergosterol content and Aflatoxin B1 by HPLC. Amongst the various nutritional constituents evaluated for nutritional losses and changes the highest nutritional loss was reported in total carotenoids (88.55%) followed by total sugars (85.5%). The protein content of the infected sample increased from 18.01% to 23%. The nutritional profile of chilli powder (Capsicum annum var. sannam L.) shows highest share of total soluble sugars (32.89%) and fiber... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Chili powder; Nutritional changes; Aflatoxin. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100024 Time course of virus-like particles (VLPs) double-stranded rna accumulation in toxigenic and non-toxigenic strains of Aspergillus flavus BJM Silva,Valéria N.; Durigon,Edison Luiz; Pires,Maria de Fátima Costa; Lourenço,Alexandre; Faria,Maria Jacinta de; Corrêa,Benedito. Two strains of Aspergillus flavus, non-toxigenic NRRL 6550 and toxigenic NRRL 5940, were studied over a period of 44 days, in order to detect the presence of virus-like particles (VLPs) by means of electron microscopy (EM) and nucleic acids electrophoresis. Only the toxigenic strain contained VLPs, presenting three-segmented dsRNA. An increase in VLPs number was observed during the exponential phase of fungal growth, up to day 12 of culture; after this, higher levels of aflatoxin production in toxigenic NRRL 5940 mycelia occurred in parallel with decreased VLPs replication. Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycovirus; Double-stranded RNA; Virus-like particles; Aspergillus flavus. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100013 Copper induction and differential expression of laccase in Aspergillus flavus BJM Gomaa,Ola M.; Momtaz,Osama A.. Aspergillus flavus was isolated from soil and exhibited laccase activity under both constitutive and copper induced conditions. Spiking the medium with 1 mM copper sulfate resulted in an increase in the activity which reached 51.84 U/mL, a distinctive protein band was detected at 60 kDa. The extracellular enzyme was purified 81 fold using gel filtration chromatography and resulted in two different laccase fractions L1 and L2, the latter had a higher enzymatic activity which reached 79.57 U/mL and specific activity of 64.17 U/μg protein. The analysis of the spectrum of the L2 fraction showed a shoulder at 330 nm which is characteristic for T2/T3 copper centers; both copper and zinc were detected suggesting that this is an unconventional white laccase.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Laccase; Copper induction; Textile waste water decolorization. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100285 Mycoflora and aflatoxin/fumonisin production by fungal isolates from freshly harvested corn hybrids BJM Almeida,Adriana P.; Corrêa,Benedito; Mallozzi,Marisa A. B.; Sawazaki,Eduardo; Soares,Lúcia M. Valente. The mycoflora of 3 hybrids of freshly harvested corn grains collected from three regions of the state of São Paulo, Brazil (Assis, Capão Bonito and Ribeirão Preto) was investigated. A total of 66 samples were analyzed focusing on the influence of abiotic factors (moisture content, water activity, temperature and rainfall) on both the prevalence of Aspergillus flavus and Fusarium moniliforme, and the ability of these genera isolates to produce aflatoxins and fumonisins, respectively. In the three surveyed regions, the fungal population comprised mainly Fusarium spp., Penicillium spp., Aspergillus spp. and 2 others filamentous fungal genera, which were isolated from corn kernels showing water activity of 0.30 to 0.99 and moisture content of 5.0% to 20.2%.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Aspergillus flavus; Fumonisins; Fusarium moniliforme; Corn. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400016 Growth and enzymatic responses of phytopathogenic fungi to glucose in culture media and soil BJM Costa,Beatriz de Oliveira; Nahas,Ely. The effect of inoculation of Aspergillus flavus, Fusarium verticillioides, and Penicillium sp. in Dystrophic Red Latosol (DRL) and Eutroferric Red Latosol (ERL) soils with or without glucose on the total carbohydrate content and the dehydrogenase and amylase activities was studied. The fungal growth and spore production in culture medium with and without glucose were also evaluated. A completely randomized design with factorial arrangement was used. The addition of glucose in the culture medium increased the growth rate of A. flavus and Penicillium sp. but not of F. verticillioides. The number of spores increased 1.2 for F. verticillioides and 8.2 times for A. flavus in the medium with glucose, but was reduced 3.5 times for Penicillium sp. The total... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fusarium verticillioides; Aspergillus flavus; Penicillium sp; Dehydrogenase; Amylase. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100039 Production of aflatoxins by Aspergillus flavus and of fumonisins by Fusarium species isolated from Brazilian sorghum BJM Silva,Josefa B. da; Dilkin,Paulo; Fonseca,Homero; Corrêa,Benedito. Fifty-nine Aspergillus flavus and 35 Fusarium verticillioides strains, isolated from freshly harvested (10) and stored (130) Brazilian sorghum samples, were tested regarding their ability to produce aflatoxins (coconut milk agar) and fumonisins (rice culture), respectively. Aflatoxins B1 and B2 were detected by TLC, and fumonisins B1 and B2 were analyzed by HPLC. Thirty-eight (64.4%) A. flavus strains produced detectable levels of aflatoxins at concentrations ranging from 12.00 to 3282.50 µg/kg (AFB1 + AFB2), while thirty two (91%) F. verticillioides strains produced FB1 at concentrations ranging from 0.12 to 5.38 µg/g. Two F. proliferatum strains produced low fumonisin levels. The toxigenic potential of A. flavus (64.4%) and F. verticillioides (91.5%)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Toxigenicity; Aspergillus flavus; Fusarium verticillioides; Mycotoxins; Sorghum; Aflatoxin; Fumonisin. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200002 Evaluation of viability of Aspergillus flavus and aflatoxins degradation in irradiated samples of maize BJM Aquino,Simone; Ferreira,Fabiane; Ribeiro,Deise Helena Baggio; Corrêa,Benedito; Greiner,Ralf; Villavicencio,Anna Lucia Casañas Haasis. One of the currently most important fungi in stored grains is Aspergillus flavus, which produce aflatoxins. This fungus can grow on diverse substrates and represents a serious public health and animal nutritional problem. Therefore, the study of techniques that can be applied to the control of aflatoxins is of great importance. The objective of the present study was to determine the effects of gamma radiation on the growth of Aspergillus flavus Link and on degradation of aflatoxin B1 and B2 (AFB1 and AFB2) at a relative humidity of 97 99% and a water activity (Aw) of 0.88-0.94. Samples of corn grains were irradiated using a cobalt 60 source emitting gamma rays at doses of 2, 5 and 10 kGy. Irradiation was found to be effective in reducing the number... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma radiation; Aspergillus flavus; Fluorescent viability test; Water activity; Maize. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400009 Inhibitory activity of compounds isolated from Polymnia sonchifolia on aflatoxin production by Aspergillus flavus BJM Pak,Adriana; Gonçalez,Edlayne; Felicio,Joana D'arc; Pinto,Marina Mori; Rossi,Maria Helena; Simoni,Isabela Cristina; Lopes,Márcia Nasser. Polymnia sonchifolia, commonly known as ";yacon";, was originally cultivated at Andes moutains in South America. Recently, the specie attracted worldwide attention because of its wide range of uses, for example in the control of diabetes melitus, besides the antifungal and pesticidal compounds were found in the leaves. This study describes the identification of two flavonoids: 3', 5, 7 trihydroxy-3, 4'-dimethoxyflavone (compound 1) and 3', 4', 5- trihydroxy-7-methoxy flavanone (compound 2) and two sesquiterpenes lactones: enhydrin (compound 3) and a mixture of enhydrin and uvedalin (compound 4) isolated from Polymnia sonchifolia leaves and their effects on the aflatoxin production by Aspergillus flavus. The identification of the compounds were achieved by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymnia sonchifolia; Flavonoids; Melampolides; Aflatoxin; Aspergillus flavus. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200018 Activity of the aqueous extract from Polymnia sonchifolia leaves on growth and production of aflatoxin B1 by Aspergillus flavus BJM Pinto,Marina M.; Gonçalez,Edlayne; Rossi,Maria H.; Felício,Joana D.; Medina,Cláudia S.; Fernandes,Maria J.B.; Simoni,Isabela C.. The aqueous extract from Polymnia sonchifolia leaves (AE) was tested for inhibitory activity on aflatoxin B1(AFB1) production and growth of Aspergillus flavus. The cytotoxicity of AE on Vero cells was also performed. Suspensions of A. flavus spores were inoculated into 50 mL of YES medium together with different concentrations of the AE. The aflatoxin B1 was extracted, analyzed by thin layer chromatography and quantified by photodensitometry. All the concentrations of AE induced inhibition of AFB1 production. The aqueous extract showed in vitro cytotoxicity to Vero cells only at concentrations above 500 µg/mL. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin B1; Aspergillus flavus; Polymnia sonchifolia; Cytotoxicity. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200011 Inhibitory effect of sodium metabisulphite and chlorine on growth of Aspergillus spp. and Penicillium spp. strains isolated from marine shrimp Ciência Rural Santos,Ygor Flávio de Moraes; Veloso,Átyla Peeter Batista; Calvet,Rodrigo Maciel; Gomes Pereira,Maria Marlúcia; Pereyra,Carina Maricel; Dalcero,Ana Maria; Torres,Adriana Mabel; Muratori,Maria Christina Sanches. The sodium metabisulphite (SMB) is used in shrimp farming to prevent melanosis and the 5.0 ppm chlorine (CL) concentration used in the shrimp processing is efficient as a bactericide, but there is no evidence of the effectiveness of these chemical compounds as fungicides. Therefore, the aim of this study was to evaluate the in vitro effect of sodium metabisulphite (SMB) and chlorine (CL) on the growth of Aspergillus and Penicillium species isolated from marine shrimp in different stages of processing. The samples were collected from a frozen shrimp processing industry, located in Piauí State, Brazil. The total fungi and occurrence of Aspergillus and Penicillium species were evaluated. For in vitro sensibility test using the diffusion disk in agar method,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; Litopenaeus vannamei; Penicillium citrinum; Shrimp culture; Conservation. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000900029 Occurrence and exposure assessment to aflatoxins in peanuts commercialized in the northwest of Parana, Brazil Ciência Rural Santos,Alini Cristini dos; Souza,Aline Amenencia de; Silva,Milena Veronezi; Nerilo,Samuel Botião; Souza,Alexandra Perdigão Maia de; Bando,Érika; Machinski Junior,Miguel. ABSTRACT: Peanuts are a nutritious food consumed worldwide. Some species of the genus Aspergillus, producers of aflatoxins, colonize peanuts. Human exposure to aflatoxins occurs by ingesting contaminated foods. The objective of this paper was to assess the occurrence and dietary exposure of the population to aflatoxins in peanuts commercially available in Maringa, Brazil, from May 2013 to April 2017. A total of 104 peanut samples were evaluated by thin-layer chromatography and confirmed by derivatization with trifluoroacetic acid. The contamination rate detected was 24.0% in average concentration of 13.4µg/kg. Twenty positive samples (19.2%) exceeded the maximum level permitted in Brazil for the sum of four aflatoxins. Estimated probable daily intake was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxins; Aspergillus flavus; Peanuts; Risk assessment; Public health. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600750 Diferentes pré-inóculos, temperaturas e tempos de incubação na produção aflatoxina B1 em arroz Ciência Rural Ritter,Ana Carolina; Noll,Isa Beatriz. Estudos indicam que um dos problemas mais sérios que afetam os manejos pré e pós-colheita do arroz é a presença de fungos das espécies Aspergillus ou Penicillium, potencialmente produtores de micotoxinas. Os objetivos deste trabalho foram avaliar a capacidade produtora de aflatoxina B1 de cepas isoladas do arroz e observar o efeito do pré-inóculo nas mesmas. Foram utilizados três isolados de Aspergillus flavus, conhecidamente já produtores, que foram testados nas temperaturas de 20 e 25°C, combinadas com tempos de incubação 11, 14 e 21 dias. Os pré-inóculos utilizados foram Yeast Extrat Sucrose (YES) e Czapeck Yeast Extrat (CYA). Todas as cepas retiradas do pré-inóculo em meio YES e inoculadas no arroz, em temperatura de 25°C/18 dias e 20°C/14 dias,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus flavus; CYA; YES; Tempo de incubação; Temperatura; Arroz. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000900022 Evaluation of antifungal activity of seaweed extracts Ciência e Agrotecnologia Peres,Julio Cesar Fernandes; Carvalho,Luciana Retz de; Gonçalez,Edlayne; Berian,Luis Otávio Saggion; Felicio,Joana D´arc. Seaweeds are subject to numerous biological interactions and sometimes to extreme abiotic conditions, so they have developed among other defense mechanisms, the ability to produce biologically active substances. Thus, these organisms produce mainly terpenes and phenols. Among others, the antifungal activity, due to its importance in human and animal health and the production of agricultural products, has been the subject of several studies. In the present work, this activity was investigated in ten seaweeds extracts, by direct bioautography assays, compared to Colletotrichum lagenarium and disk diffusion assay, compared to Aspergillus flavus. The organisms studied were: Stypopodium zonale, Laurencia dendroidea, Ascophyllum nodosum, Sargassum muticum,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Etanolic extract; Colletotrichum lagenarium; Aspergillus flavus. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542012000300004 Avaliação da micoflora e ocorrência de micotoxinas em cascas de amendoim em diferentes estágios de maturação da vagem Ciência e Agrotecnologia Gonçalez,Edlayne; Souza,Tiago Noel de; Rossi,Maria Helena; Felicio,Joana D'arc; Corrêa,Benedito. As cascas de amendoim (Arachis hypogaea L.) são de grande importância para confecção de cama de frangos, de gado de leite e como fonte de fibras para ruminantes, portanto a elucidação dos mecanismos de contaminação por fungos toxigênicos e por micotoxinas em amendoim é imprescindível, especialmente para que medidas preventivas possam ser tomadas. Realizou-se, este trabalho, em Junqueirópolis, Estado de São Paulo, Brasil. Os principais fungos isolados nas cascas de amendoim foram Fusarium ssp. (78,75 %), Rhizopus ssp. (14,1 %) e A. flavus (11,75 %). No solo foram isolados Penicillium spp., Fusarium spp. e Aspergillus flavus, entre outros. Aflatoxinas foram detectadas em amostras de cascas de amendoim a partir do estágio de granação em concentrações que... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxinas; Ácido ciclopiazônico; Fumonisinas; Aspergillus flavus; Amendoim; Arachis hypogaea L. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542008000500004 Registros recuperados: 38 Primeira ... 1 2 ... Última

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