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Development of a mreB-targeted real-time PCR method for the quantitative detection of Vibrio harveyi in seawater and biofilm from aquaculture systems ArchiMer
Mougin, Julia; Roquigny, Roxane; Travers, Marie-agnes; Grard, Thierry; Bonnin-jusserand, Maryse; Le Bris, Cédric.
Vibrio harveyi is a particularly problematic Gram-negative bacterium because it can form biofilms on aquaculture facility surfaces, leading to resistance of bacteria against antibiotics and water sanitizers. A SYBR Green I quantitative real-time PCR method was developed to detect V. harveyi directly from environmental samples, including seawater and biofilm. Specific primers targeting the mreB gene were designed. The exclusivity and inclusivity of the newly designed primers were evaluated using a panel of 85 bacteria: 58 V. harveyi from multiples origins and 27 non-V. harveyi isolates, and compared with two pairs of primers targeting the topA and toxR genes that were designed previously. All sets of primers were able to distinguish V. harveyi from closely...
Tipo: Text Palavras-chave: Vibrio harveyi; Quantitative detection; MreB gene; Seawater; Biofilm; Aquaculture.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00622/73365/72542.pdf
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The oceans are changing: impact of ocean warming and acidification on biofouling communities ArchiMer
Dobretsov, Sergey; Coutinho, Ricardo; Rittschof, Daniel; Salta, Maria; Ragazzola, Federica; Hellio, Claire.
Climate change (CC) is driving modification of the chemical and physical properties of estuaries and oceans with profound consequences for species and ecosystems. Numerous studies investigate CC effects from species to ecosystem levels, but little is known of the impacts on biofilm communities and on bioactive molecules such as cues, adhesives and enzymes. CC is induced by anthropogenic activity increasing greenhouse emissions leading to rises in air and water temperatures, ocean acidification, sea level rise and changes in ocean gyres and rainfall patterns. These environmental changes are resulting in alterations within marine communities and changes in species ranges and composition. This review provides insights and synthesis of knowledge about the...
Tipo: Text Palavras-chave: Biofilm; Biofouling; Bioactive compounds; Climate change.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61686/65641.pdf
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Sponge-Inspired Dibromohemibastadin Prevents and Disrupts Bacterial Biofilms without Toxicity ArchiMer
Le Norcy, Tiffany; Niemann, Hendrik; Proksch, Peter; Tait, Karen; Linossier, Isabelle; Rehel, Karine; Hellio, Claire; Fay, Fabienne.
Since the banning of several families of compounds in antifouling (AF) coatings, the search for environmentally friendly AF compounds has intensified. Natural sources of AF compounds have been identified in marine organisms and can be used to create analogues in laboratory. In a previous study, we identified that dibromohemibastadin-1 (DBHB) is a promising AF molecule, leading to the inhibition of the activity of phenoloxidase, an enzyme involved in the attachment of mussels to surfaces. This paper describes the activity of the DBHB on biofilm formation and its detachment and on bacterial adhesion and communication: quorum sensing. DBHB has an anti-biofilm activity without affecting adhesion of marine and terrestrial bacteria at a dose of 10 mu M....
Tipo: Text Palavras-chave: Biofouling; Biofilm; Bastadin derivative; Microfouling; Quorum sensing; Sponge.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00616/72813/72581.pdf
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Does the size of the microphytobenthic biofilm on intertidal muflats depend on the available photosynthetic biomass? ArchiMer
Herlory, Olivier; Blanchard, G; Planche, S; Huet, V; Richard, Pierre.
Many scientists consider that the top centimetre of the sediment on intertidal mudflats contains the photosynthetic competent biomass (PCB). Part of this biomass migrates upward to the surface of the sediment during diurnal emersion periods to form a temporary biofilm: the photosynthetic active biomass (PAB). The present study tests the hypothesis that the size of the biofilm (PAB) is functionally dependent on PCB. Therefore, we have plotted PAB as a function of PCB for a range of different environmental conditions: 3 seasons and 5 tidal situations, representing a total of 600 cores. This investigation points out that, in spring and autumn, there was a simple linear relationship between PAB and PCB, thus indicating that the maximum size of the biofilm...
Tipo: Text Palavras-chave: Functional relationship; Biofilm; Biomass; Intertidal mudflat; Microphytobenthos.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-3622.pdf
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Exploring Antifouling Activity of Biosurfactants Producing Marine Bacteria Isolated from Gulf of California ArchiMer
Alemán-vega, Monserrat; Sánchez-lozano, Ilse; Hernández-guerrero, Claudia J.; Hellio, Claire; Quintana, Erika T..
Biofouling causes major problems and economic losses to marine and shipping industries. In the search for new antifouling agents, marine bacteria with biosurfactants production capability can be an excellent option, due to the amphipathic surface-active characteristic that confers antimicrobial and antibiofilm activities. The aim of this study was to evaluate the antifouling activity of biosurfactants producing marine bacteria from the Gulf of California. The cell free culture supernatant (CFCS) of Bacillus niabensis (S-69), Ralstonia sp. (S-74) (isolated from marine sediment) and of B. niabensis (My-30) (bacteria associated to the sponge Mycale ramulosa) were screened for production of biosurfactants (using hemolysis and drop collapse test, oil...
Tipo: Text Palavras-chave: Adhesion; Biofilm; Biosurfactants; Environmentally friendly antifouling; Field assays; Bacillus niabensis; Ralstoniasp.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00646/75804/76733.pdf
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Determination of stocking density limits for Crassostrea gigas larvae reared in flow-through and recirculating aquaculture systems and interaction between larval density and biofilm formation ArchiMer
Asmani, Katia; Petton, Bruno; Le Grand, Jacqueline; Mounier, Jerome; Robert, Rene; Nicolas, Jean-louis.
The first aim of this study was to determine the stocking density limits for Pacific oyster Crassostrea gigas larvae reared in flow-through system (FTS) and recirculating aquaculture systems (RAS). The second aim was to examine biofilm formation on the larval tank wall and its interaction with larvae growth. Three larvae concentrations were tested: 50, 150, and 300 mL−1. Chemical parameters and larvae performance were measured. The biofilm was observed by scanning electron microscopy, and its bacterial composition was investigated by pyrosequencing analysis of part of the 16S rRNA gene. The highest growth (13 µm day–1), survival (87%) and metamorphosis (50%) rates were observed in FTS at 50 larvae mL–1, while lower and similar performances occurred at...
Tipo: Text Palavras-chave: Pacific oyster; Larval culture; High stocking density; Biofilm; Filamentous bacteria.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00395/50611/51324.pdf
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Microstructure of microphytobenthic biofilm and its spatio-temporal dynamics in an intertidal mudflat (Aiguillon Bay, France) ArchiMer
Herlory, Olivier; Guarini, Jean-marc; Richard, Pierre; Blanchard, Gerard.
The spatio-temporal dynamics of the microphytobenthic biofilm was analysed at microscale over a complete day-time emersion period in an intertidal mudflat. The structures of the biofilm were monitored by analysing the variability of the epipelic diatom cover in the horizontal dimension and the kinetics of biomass dispersion in the vertical dimension. Colonisation of the sediment surface by diatoms was rapid (within 15 min); 75% of the biomass contained in the top 1 mm was concentrated in the upper 200 mum, but the cover never reached 100%. This biomass had a higher chlorophyll a/pheopigment ratio than deeper in the sediment, suggesting a better physiological state and a higher photosynthetic potential. The dynamics of the biomass in the upper 200 mum...
Tipo: Text Palavras-chave: Microphytobenthos; Biofilm; Intertidal mudflat; Spatio temporal dynamics; Microscale.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00000/10905/7580.pdf
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Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers ArchiMer
Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Compere, Chantal; Combes, D.
Aims: The nature of exopolymers involved in the adhesion of a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand the antifouling potential of subtilisin. Methods and Results: The exopolymers of D41 produced by fermentation were analysed by FTIR and SDS-PAGE showing the presence of polysaccharides, glycoproteins and proteins. A high content of proteins was detected both in soluble and capsular fractions. The microscopic observations of fluorescamine and calcofluor stained adhered D41 indicated mainly the presence of proteins in exopolymers produced during adhesion. Subtilisin, the broad spectrum protease, tested in natural sea water and in polystyrene microplates showed that antifouling activity was...
Tipo: Text Palavras-chave: Subtilisin; Pseudoalteromonas; Exopolymer; Capsular protein; Biofilm; Antifouling.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4624.pdf
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Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods ArchiMer
Takahashi, Eri; Ledauphin, Jerome; Goux, Didier; Orvain, Francis.
There is no universal method that can be applied to extract bound extracellular polymeric substances (EPS) from benthic diatoms of intertidal sediments without causing cell lysis. Six extraction methods were tested on a diatom culture of Navicula jeffreyi to establish the best compromise between high yields of carbohydrate extraction and minimum cell lysis. Extraction with distilled water provoked cell lysis (as already known). The five other extraction methods (dowex resin, artificial seawater of half salinity and extractions after pretreatment with gluteraldehyde by three methods: water, dowex water and dowex buffer) did not provoke cell lysis as shown by transmission electronic microscopy. This result was confirmed by the minimum release of internal...
Tipo: Text Palavras-chave: Transmission electron microscopy; Protein; Navicula jeffreyi; Intertidal sediments; Immuno staining; Immunostaining; GCMS analysis; Dowex; Cell lysis; Biofilm.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7418.pdf
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Novel uncultured Epsilonproteobacteria dominate a filamentous sulphur mat from the 13 degrees N hydrothermal vent field, East Pacific Rise ArchiMer
Moussard, Hélène; Corre, Erwan; Cambon-bonavita, Marie-anne; Fouquet, Yves; Jeanthon, Christian.
Rapid growth of microbial sulphur mats have repeatedly been observed during oceanographic cruises to various deep-sea hydrothermal vent sites. The microorganisms involved in the mat formation have not been phylogenetically characterized, although the production of morphologically similar sulphur filaments by a Arcobacter strain coastal marine has been documented. An in situ collector deployed for 5 days at the 13 degrees N deep-sea hydrothermal vent site on the East Pacific Rise (EPR) was rapidly colonized by a filamentous microbial mat. Microscopic and chemical analyses revealed that the mat consisted of a network of microorganisms embedded in a mucous sulphur-rich matrix. Molecular surveys based on 16S rRNA gene and aclB genes placed all the...
Tipo: Text Palavras-chave: FISH; Arcobacter; Epsilonproteobacteria; 16S rRNA gene; Biofilm; Deep sea hydrothermal vent.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2110.pdf
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Kinetics of conditioning layer formation on stainless steel ArchiMer
Compere, Chantal; Bellon Fontaine, M.-n.; Bertrand, P; Costa, D; Marcus, P; Poleunis, C; Pradier, C.m.; Rondot, B; Walls, M.g..
Adhesion of micro-organisms to surfaces in marine environments leads to biofouling. The deleterious effects of biofilm growth in the marine environment are numerous and include : energy losses due to increased fluid frictional resistance or to increased heat transfer resistance, risk of corrosion induced by micro-organisms, loss of optical properties, quality control and safety problems. Antifouling agents are generally used to protect surfaces from being affected by such a biofilm. These agents are toxic and can be persistent, causing harmful environmental and ecological effects. Moreover, the use of biocides and regular cleaning considerably increase the maintenance costs of marine industries. An improved knowledge of the biofilm adhesion mechanisms is...
Tipo: Text Palavras-chave: Biofilm; Surface characterisation; Carbohydrates; Protein; Seawater; Conditioning layer.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-1701.pdf
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Vibrio tapetis, the Causative Agent of Brown Ring Disease, Forms Biofilms with Spherical Components ArchiMer
Rodrigues, Sophie; Paillard, Christine; Le Pennec, Gael; Dufour, Alain; Bazire, Alexis.
Vibrio tapetis is a marine bacterium causing Brown Ring Disease (BRD) in the Manila clam Ruditapes philippinarum. V. tapetis biofilm formation remains unexplored depite the fact that it might be linked to pathogenicity. Our objectives were to characterize the in vitro biofilm formation of V tapetis and evaluate the effects of culture conditions. Biofilm structure and its matrix composition were examined by confocal laser scanning microscopy and scanning electron microscopy. V tapetis was able to form biofilms on a glass substratum within 24 h. Polysaccharides and extracellular DNA of the biofilm matrixes were differently distributed depending on the V tapetis strains. Spherical components of about 1-2 mu m diameter were found at the biofilm surface. They...
Tipo: Text Palavras-chave: Vibrio tapetis; Biofilm; Brown Ring Disease; Ruditapes philippinarum; Spherical components; Temperature effect; Salinity effect.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00307/41783/42796.pdf
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First steps of biofilm formation on stainless steels in natural seawater ArchiMer
Bellon-fontaine, M.-n.; Bertrand, P; Compere, Chantal; Costa, D; Guezennec, Jean; Marcus, P; Poleunis, C.; Pradier, C.-m.; Rondot, B; Walls, M.g..
Numerous works are reported in literature concerning the biofilm growth on surfaces in seawater but the understanding of the adhesion mechanisms in the first steps of immersion is still subject to investigations. A knowledge of the chemical composition and of the kinetics of conditioning layer formation is a central requirement for a sound scientific understanding of biofilm growth. Due to its complexity and to the interdisciplinary nature of the subject, this research asks for the collaboration between the fields of material science, surface analyses, chemistry, marine microbiology and biochemistry. The first steps of biofilm formation on 316L stainless steels immersed in natural sea water have been studied, using a broad range of surface characterisation...
Tipo: Text Palavras-chave: Sea water; Substrates; Biofilm.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/acte-1699.pdf
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Increasing Reliability: Smart Biofouling Prevention Systems ArchiMer
Delauney, Laurent; Ralston, Emilie; Zargiel-hunsucker, Kelli.
Tipo: Text Palavras-chave: Biofilm; Biofouling; Camera; In situ; Lights; Long term; Marine; Monitoring; Optical; Protection; Sensors..
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00630/74233/73867.pdf
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Biomimetic Approaches for the Development of New Antifouling Solutions: Study of Incorporation of Macroalgae and Sponge Extracts for the Development of New Environmentally-Friendly Coatings ArchiMer
Sánchez-lozano, Ilse; Hernández-guerrero, Claudia Judith; Muñoz-ochoa, Mauricio; Hellio, Claire.
Biofouling causes major economic losses in the maritime industry. In our site study, the Bay of La Paz (Gulf of California), biofouling on immersed structures is a major problem and is treated mostly with copper-based antifouling paints. Due to the known environmental effect of such treatments, the search for environmentally friendly alternatives in this zone of high biodiversity is a priority to ensure the conservation and protection of species. The aim of this work was to link chemical ecology to marine biotechnology: indeed, the natural defense of macroalgae and sponge was evaluated against biofoulers (biofilm and macrofoulers) from the same geographical zone, and some coatings formulation was done for field assays. Our approach combines in vitro and...
Tipo: Text Palavras-chave: Adhesion; Algae; Antifouling; Biofilm; Extracts; Field assays; Sponges; Haliclona caerulea; Laurencia gardneri; Sargassum horridum; Ircinia sp.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00589/70074/68047.pdf
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Bacterial biofilms with emphasis on coagulase-negative staphylococci J. Venom. Anim. Toxins incl. Trop. Dis.
Oliveira,A; Cunha,MLRS.
In addition to their capacity to attach to surfaces, various groups of microorganisms also produce an extracellular polymeric substance known as "slime". This slime forms a thin layer around cells known as biofilm. Thus, biofilm structure comprises bacterial cells and an extracellular polymeric substance. It also presents a defined architecture, providing the microorganisms with an excellent protective environment and favoring the exchange of genetic material between cells as well as intercellular communication. The ability to produce biofilm is observed in a large group of bacteria, including coagulase-negative staphylococci (CNS) which are the predominant microorganisms of normal skin flora and have been implicated as the causative agents of hospital...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coagulase-negative staphylococci; Biofilm; Slime; Infection; Microbiology.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400003
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RESPUESTA DE LA CLOROFILA Y EL METABOLISMO DE UN ARROYO ANDINO AL AUMENTO DE TEMPERATURA EN UN EXPERIMENTO ex situ Acta biol.Colomb.
INFANTE CANGREJO,Viviana; DONATO RONDÓN,Jhon Charles.
RESUMEN En los trópicos, el cambio climático puede significar un incremento en la temperatura que puede afectar la diversidad biológica de los ecosistemas fluviales, favoreciendo la llegada de especies propias de aguas más cálidas. La variación de temperatura puede también afectar las relaciones tróficas y el metabolismo del sistema. En el presente estudio, se analizó el efecto de 2-4 °C de aumento de la temperatura del agua sobre el ensamble de diatomeas y el metabolismo del río Tota (alta montaña andina). Para este propósito, se utilizaron canales artificiales de recirculación, en los que diariamente se midieron variables fisicoquímicas (pH, conductividad, temperatura, oxígeno disuelto y velocidad del agua), se recolectaron e identificaron diatomeas, se...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arroyo Neotropical; Biofilm; Cambio climático; Canales recirculantes.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000200008
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Distribución de genes de adhesión y regulación de biofilm en Staphylococcus aureus ABCL
Cruz Martínez,Sofía Francisca; Tapia Pastrana,Gabriela; Castañón Sánchez,Carlos Alberto.
La formación de biofilms es un importante factor de virulencia que contribuye en la cronicidad de los procesos infecciosos producidos por Staphylococcus aureus. Las proteínas presentes en su superficie pueden promover la formación de biofilm. En este estudio se comparó la distribución de genes que codifican para proteínas de adhesión asociadas con la formación de biofilm en cepas resistentes (MRSA) y sensibles a meticilina (MSSA). Al analizar un total de 106 aislados obtenidos de muestras sólidas y líquidas recuperados de un hospital de México, se determinó que la formación de biofilm está asociada de manera significativa a cepas MRSA (83%). Mediante la reacción en cadena de la polimerasa (PCR), se buscó la presencia de nueve genes de adhesinas (eno, ebps,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Biofilm; Resistencia a meticilina; Gen cna; Adhesión.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572016000400020
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Histatin 5 and human lactoferrin inhibit biofilm formation of a fluconazole resistant Candida albicans clinical isolate Anais da ABC (AABC)
CURVELO,JOSÉ A.R.; MORAES,DANIEL C. DE; ANJOS,CAMILA A. DOS; PORTELA,MARISTELA B.; SOARES,ROSANGELA M.A..
Abstract:Candida albicans is the most important fungal pathogen that causes infections in humans. Biofilms are hard-to-treat structures due to their high antifungal resistance. Saliva is a fluid that contains antimicrobial substances acting as the first-line of defense against pathogens, and its immune components may be potential tools for the discovery of new treatments against candidiasis. To evaluate the activity of histatin 5 and human lactoferrin against biofilm formation. A fluconazole-resistant Candida albicans clinical isolate was used as the model microorganism. Morphogenesis was evaluated by differential counting. Biofilm quantification was performed by XTT reduction assay. Thickness and topography of biofilms were assessed through confocal laser...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Candida albicans; Fluconazole resistance; Histatin 5; Lactoferrin.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100622
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Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis Anais da ABC (AABC)
COSTA,GÉSSICA A.; ROSSATTO,FERNANDA C.P.; MEDEIROS,ALINE W.; CORREA,ANA PAULA F.; BRANDELLI,ADRIANO; FRAZZON,ANA PAULA G.; MOTTA,AMANDA DE S. DA.
ABSTRACT The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biofilm; Crystal violet; Food industry; MTT assay; Peptide P34.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100073
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